The method 1, further comprising {TAIL ~ the step}, during or after step , of quantifying {HEAD ~ the number} of microcolonies 
The method 1 , further comprising { TAIL ~ the step } , during or after step , of quantifying { HEAD ~ the number } of microcolonies
OTH OTH OTH OTH OTH OTH TAIL TAIL TAIL TAIL TAIL TAIL OTH OTH OTH OTH OTH OTH OTH OTH HEAD HEAD HEAD HEAD HEAD HEAD OTH OTH