An aliquot of {HEAD ~ the sample solution} containing {TAIL ~ the liberated nucleic acids} in the lysis, permeabilization or transport medium is added to the second solution, and mixed vigorously 
An aliquot of { HEAD ~ the sample solution } containing { TAIL ~ the liberated nucleic acids } in the lysis , permeabilization or transport medium is added to the second solution , and mixed vigorously
OTH OTH OTH HEAD HEAD HEAD HEAD HEAD HEAD HEAD REL TAIL TAIL TAIL TAIL TAIL TAIL TAIL TAIL OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH