The introduction of the unsaturated R group in the dye molecule is preferably carried out by {HEAD ~ a reaction} between a Rhodamine having a free carboxyl group in position 2′, and {TAIL ~ an unsaturated halogenated derivative}, performing the reaction in the presence of a base, such as anhydrid solid carbonate 
between
The introduction of the unsaturated R group in the dye molecule is preferably carried out by { HEAD ~ a reaction } between a Rhodamine having a free carboxyl group in position 2′ , and { TAIL ~ an unsaturated halogenated derivative } , performing the reaction in the presence of a base , such as anhydrid solid carbonate
OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH HEAD HEAD HEAD HEAD HEAD HEAD REL OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH TAIL TAIL TAIL TAIL TAIL TAIL TAIL TAIL OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH