In order to determine precise T cell epitopes by, for example, fine mapping techniques, a peptide having {HEAD ~ Der p VII} or {TAIL ~ Der f VII T cell stimulating activity} and thus comprising at least one T cell epitope as determined by T cell biology techniques is modified by addition or deletion of amino acid residues at either the amino or carboxy terminus of the peptide and tested to determine a change in T cell reactivity to the modified peptide 
In order to determine precise T cell epitopes by , for example , fine mapping techniques , a peptide having { HEAD ~ Der p VII } or { TAIL ~ Der f VII T cell stimulating activity } and thus comprising at least one T cell epitope as determined by T cell biology techniques is modified by addition or deletion of amino acid residues at either the amino or carboxy terminus of the peptide and tested to determine a change in T cell reactivity to the modified peptide
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