The invention includes {HEAD ~ the use} of agitation to enhance the penetration of an effective amount of {TAIL ~ cryoprotectant} 
The invention includes { HEAD ~ the use } of agitation to enhance the penetration of an effective amount of { TAIL ~ cryoprotectant }
OTH OTH OTH HEAD HEAD HEAD HEAD HEAD HEAD OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH TAIL TAIL TAIL TAIL TAIL