The invention involves immersing a mammalian tissue or cultured tissue equivalent in a cryoprotectant solution, agitating the cryoprotectant solution and the immersed tissue to achieve effective penetration of the cryoprotectant solution into the tissue, and then freezing the tissue at {HEAD ~ a very slow freezing rate} at {TAIL ~ 0.3° C.} or less/min 
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The invention involves immersing a mammalian tissue or cultured tissue equivalent in a cryoprotectant solution , agitating the cryoprotectant solution and the immersed tissue to achieve effective penetration of the cryoprotectant solution into the tissue , and then freezing the tissue at { HEAD ~ a very slow freezing rate } at { TAIL ~ 0.3 ° C. } or less / min
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