The L, V, , J DNA fragments were isolated using PCR and cloned into {TAIL ~ CHO cell expression vectors}, pTCSLCκDHFR and {HEAD ~ pTCSLCg1NeoAp}, which carried human kappa and IgG1 constant region cONA, respectively, for mouse V/human C chimeric antibody expression 
The L , V , , J DNA fragments were isolated using PCR and cloned into { TAIL ~ CHO cell expression vectors } , pTCSLCκDHFR and { HEAD ~ pTCSLCg1NeoAp } , which carried human kappa and IgG1 constant region cONA , respectively , for mouse V / human C chimeric antibody expression
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