{HEAD ~ The method} 1, further comprising {TAIL ~ the step}, during or after step , of quantifying the number of microcolonies 
comprising
{ HEAD ~ The method } 1 , further comprising { TAIL ~ the step } , during or after step , of quantifying the number of microcolonies
HEAD HEAD HEAD HEAD HEAD HEAD OTH OTH OTH REL TAIL TAIL TAIL TAIL TAIL TAIL OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH