The introduction of {HEAD ~ the unsaturated R group} in {TAIL ~ the dye molecule} is preferably carried out by a reaction between a Rhodamine having a free carboxyl group in position 2′, and an unsaturated halogenated derivative, performing the reaction in the presence of a base, such as anhydrid solid carbonate 
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The introduction of { HEAD ~ the unsaturated R group } in { TAIL ~ the dye molecule } is preferably carried out by a reaction between a Rhodamine having a free carboxyl group in position 2′ , and an unsaturated halogenated derivative , performing the reaction in the presence of a base , such as anhydrid solid carbonate
OTH OTH OTH HEAD HEAD HEAD HEAD HEAD HEAD HEAD HEAD REL TAIL TAIL TAIL TAIL TAIL TAIL TAIL OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH