{TAIL ~ The introduction} of the unsaturated R group in the dye molecule is preferably carried out by a reaction between a Rhodamine having a free carboxyl group in position 2′, and an unsaturated halogenated derivative, performing the reaction in the presence of {HEAD ~ a base}, such as anhydrid solid carbonate 
{ TAIL ~ The introduction } of the unsaturated R group in the dye molecule is preferably carried out by a reaction between a Rhodamine having a free carboxyl group in position 2′ , and an unsaturated halogenated derivative , performing the reaction in the presence of { HEAD ~ a base } , such as anhydrid solid carbonate
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