{HEAD ~ The method} 1, wherein the amplification is by {TAIL ~ quantitative reverse transcriptase polymerase chain reaction} 
{ HEAD ~ The method } 1 , wherein the amplification is by { TAIL ~ quantitative reverse transcriptase polymerase chain reaction }
HEAD HEAD HEAD HEAD HEAD HEAD OTH OTH OTH OTH OTH OTH OTH TAIL TAIL TAIL TAIL TAIL TAIL TAIL TAIL TAIL TAIL