The sonication duration is {HEAD ~ preferably less than 15 minutes}, {TAIL ~ more preferably less than 6 minutes}, most preferably 60 seconds or less 
The sonication duration is { HEAD ~ preferably less than 15 minutes } , { TAIL ~ more preferably less than 6 minutes } , most preferably 60 seconds or less
OTH OTH OTH OTH HEAD HEAD HEAD HEAD HEAD HEAD HEAD HEAD HEAD OTH TAIL TAIL TAIL TAIL TAIL TAIL TAIL TAIL TAIL TAIL OTH OTH OTH OTH OTH OTH OTH