{HEAD ~ The invention} includes {TAIL ~ the use} of agitation to enhance the penetration of an effective amount of cryoprotectant 
includes
{ HEAD ~ The invention } includes { TAIL ~ the use } of agitation to enhance the penetration of an effective amount of cryoprotectant
HEAD HEAD HEAD HEAD HEAD HEAD REL TAIL TAIL TAIL TAIL TAIL TAIL OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH