The presence of the intermediate layer allows the cutting tool to safely and completely cut a spot and remove the cut spot from the gel or membrane without scratching or otherwise damaging the surface, and with {HEAD ~ at most a temporary depression} in {TAIL ~ the intermediate layer} 
The presence of the intermediate layer allows the cutting tool to safely and completely cut a spot and remove the cut spot from the gel or membrane without scratching or otherwise damaging the surface , and with { HEAD ~ at most a temporary depression } in { TAIL ~ the intermediate layer }
OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH HEAD HEAD HEAD HEAD HEAD HEAD HEAD HEAD HEAD REL TAIL TAIL TAIL TAIL TAIL TAIL TAIL