{TAIL ~ The invention} involves immersing a mammalian tissue or cultured tissue equivalent in a cryoprotectant solution, agitating the cryoprotectant solution and the immersed tissue to achieve effective penetration of the cryoprotectant solution into the tissue, and then freezing the tissue at a very slow freezing rate at 0.3° C. or {HEAD ~ less/min} 
{ TAIL ~ The invention } involves immersing a mammalian tissue or cultured tissue equivalent in a cryoprotectant solution , agitating the cryoprotectant solution and the immersed tissue to achieve effective penetration of the cryoprotectant solution into the tissue , and then freezing the tissue at a very slow freezing rate at 0.3 ° C. or { HEAD ~ less / min }
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