The concentrations are based on use of {TAIL ~ Mediunm 199 liquid}, {HEAD ~ Earles Balanced Salts} and Hepes 
The concentrations are based on use of { TAIL ~ Mediunm 199 liquid } , { HEAD ~ Earles Balanced Salts } and Hepes
OTH OTH OTH OTH OTH OTH OTH TAIL TAIL TAIL TAIL TAIL TAIL TAIL OTH HEAD HEAD HEAD HEAD HEAD HEAD HEAD OTH OTH