The method 1, further comprising {HEAD ~ the step}, during or after step , of quantifying the number of {TAIL ~ microcolonies} 
The method 1 , further comprising { HEAD ~ the step } , during or after step , of quantifying the number of { TAIL ~ microcolonies }
OTH OTH OTH OTH OTH OTH HEAD HEAD HEAD HEAD HEAD HEAD OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH TAIL TAIL TAIL TAIL TAIL