The introduction of {HEAD ~ the unsaturated R group} in the dye molecule is preferably carried out by a reaction between a Rhodamine having a free carboxyl group in {TAIL ~ position} 2′, and an unsaturated halogenated derivative, performing the reaction in the presence of a base, such as anhydrid solid carbonate 
The introduction of { HEAD ~ the unsaturated R group } in the dye molecule is preferably carried out by a reaction between a Rhodamine having a free carboxyl group in { TAIL ~ position } 2′ , and an unsaturated halogenated derivative , performing the reaction in the presence of a base , such as anhydrid solid carbonate
OTH OTH OTH HEAD HEAD HEAD HEAD HEAD HEAD HEAD HEAD OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH TAIL TAIL TAIL TAIL TAIL OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH OTH