Can keratin 8 and 17 immunohistochemistry be of diagnostic value in cervical cytology?
The use of the keratin 8 antibody in normal cervical smears enabled the detection of endocervical cells in cases where they were thought to be absent, particularly in cases with severe inflammation. Staining with keratin 17 enabled the identification of reserve cells or immature metaplastic cells, which were often misinterpreted as parabasal cells. The application of antibodies to these subtypes of keratins in cervical cytology can to a certain extent help in the identification of CIN and may in future be tested in automated screening.
The resistance of freshly adherent endothelial cells to detachment by shear stress is matrix- (fibronectin approximately equal to vitronectin > laminin approximately equal to COL) and time-dependent. Fibronectin provided the greatest cell retention in the least amount of time.