Does asymmetric PCR increase efficiency of melting peak analysis on the LightCycler?
Asymmetric PCR resulting in the formation of higher amounts of target strands significantly increases the efficiency of LightCycler allelic-discrimination.
Because the model can produce a range of service recommendations, the assumptions that inform it should be clearly stated and justified. This method makes the assumptions on which services are planned explicit and allows for a rational approach to decision making.