Does constitutive homologous expression of phosphoglucomutase and transaldolase increase the metabolic flux of Fusarium oxysporum?
Homologous overexpression of phosphoglucomutase and transaldolase in F. oxysporum was shown to enhance the growth characteristics of the strain in both xylose and glucose in aerobic conditions. The intracellular metabolites profile indicated how the changes in the metabolome could have resulted in the observed growth characteristics.
The results suggest that beta1 integrin is tyrosine phosphorylated and links with actin via alpha-actinin in HBE cells but prevented from linking with actin in MCF-7 cells by phosphorylation at both tyrosine and serine/threonine of beta1 integrin which forms a complex with alpha-actinin and CaMKII. Thus the linkage formation of beta1 integrin with actin may be differentially regulated by its tyrosine and serine/threonine phosphorylation in normal HBE cells and breast cancer MCF-7 cells.