Does electroporation facilitate introduction of reporter transgenes and virions into schistosome eggs?
Square wave electroporation facilitates introduction of transgenes into the schistosome egg. Electroporation was more effective for the transduction of eggs with pseudotyped MLV than simply soaking the eggs in virions. These findings underscore the potential of targeting the schistosome egg for germ line transgenesis.
The prolonged latencies suggest a defect in the afferent pathway from the rectum in children with chronic constipation and encopresis.