Does oligonucleotide treatment cause flax β-glucanase up-regulation via changes in gene-body methylation?
To our best knowledge, this is the first report on plant gene activation by treatment with oligodeoxynucleotides homologous to the coding region of the gene. Apart from the evident effectiveness, the most important issue is that the EMO method allows generation of favourably altered plants, whose cultivation makes the plant producer independent from the complicated procedure of obtaining an agreement on GMO release into the environment and whose products might be more easily introduced to the global market.
This results indicate that pre-operative blood testing in children undergoing minor surgical procedures has very limited value in patient management. It may be unpleasant for the patient and parents. A careful history and physical examination are of greater importance than routine laboratory test in determining a child's fitness for surgery.