Does generation of Platelet Microparticles After Cryopreservation of Apheresis Platelet concentrate Contribute to the Hemostatic Activity?
Our results showed that, after cryopreservation, while levels of PMPs were increasing, significantly higher and earlier thrombin formation was occurring in the samples analysed, despite the significant decrease in the viability. Considering the damage caused by the freezing process and the scarcity of evidence for their in vivo superiority, frozen platelets shall be considered for use in austere environments, reserving fresh platelets for prophylactic use in blood banks.
These data suggest that vibratory load may play an important role in extracellular matrix metabolism of intervertebral disc cells, especially in the gene expression pathway of proteoglycans. It has been proposed that vibratory loading increases production of matrix-degrading, proteolytic enzymes. We have demonstrated that gene expression for key matrix messages and matrix metalloproteinase is decreased by vibration. In conclusion, we believe that study of the roles between extracellular matrix gene suppression and mechanical stress may clarify the pathomechanism of disc degeneration, such as disc herniation or degeneration.