Is intracellular IFNgamma production in CD3 negative cells exposed to allo-antigens an indicator of prior sensitization?
The CFC assay measures IFNgamma production in CD3- cells that may indicate a memory response to allo-Ags. This response is limited to HS patients and normal females with previous PG. Patients undergoing AMR show significantly higher reactivity. This assay may represent a novel approach to measurement of allo-sensitization with clinical utility in predicting those at risk for AMR.
Our results indicate that chitosan could be a promising agent for further evaluations in antitumor treatments targeting melanoma.