Is cryopreservation of rat precision-cut liver slices associated with major metabolic stress and ionic perturbations?
The lack of restoration of potassium/sodium exchange might be a primary event in the metabolic alterations occurring in cryopreserved PCLS after thawing, that contributes to further mitochondrial alterations, caspase activation and compromises ATP-dependent anabolic pathways.
One of the most frequent pathogens involved in respiratory tract infections are: Pseudomonas aeruginosa and Staphylococcus aureus. Most patients hospitalized at Intensive Care Units were in carrier state. These complications extend the rehabilitation process and generate high costs. It is necessary to create standards of care of patients with tracheostomy after head injury.