Does low-density lipoprotein-mediated delivery of docosahexaenoic acid selectively kill murine liver cancer cells?
The engineered LDL-DHA nanoparticles possessed enhanced physical and oxidative stabilities over native LDL and free DHA. Dose-response studies showed that therapeutic doses of LDL-DHA nanoparticles that completely killed TIB-75 were innocuous to TIB-73. The selective induction of lipid peroxidation and reactive oxygen species in the cancer cells was shown to play a central role in LDL-DHA nanoparticle-mediated cytotoxicity.
This study points to an essential role of medium composition for optimized growth and differentiation of primary organotypic cultures.