Are substrate affinity and catalytic efficiency improved by decreasing glycosylation sites in Trichoderma reesei cellobiohydrolase I expressed in Pichia pastoris?
Post-translational glycan-modification of CBH I in P. pastoris has different impacts on the properties of the secreted enzymes. Substrate affinity and catalytic efficiency were improved significantly while the activity and high temperature stability were negatively affected.
The geographical location of the department under study and the changes in administrative units created a "natural experiment" that showed major changes in paediatric hospital-seeking behaviour. Within the RSD, the free choice of hospitals seemed to work, whereas the new boundary between the CDR and the RSD meant that paediatric patients were admitted at hospitals situated in the CDR even though the distances to these hospitals were considerably longer than those to other hospitals. One could question whether patients really have a free choice across administrative borders as political and economic concerns seemed to outweigh free patient choice.