Do deep sequencing and microarray hybridization identify conserved and species-specific microRNAs during somatic embryogenesis in hybrid yellow poplar?
Solexa sequencing and microarray hybridization were used to discover 232 candidate conserved miRNAs from 61 miRNA families and 273 candidate species-specific miRNAs in hybrid yellow poplar. In these predicted miRNAs, 64 conserved miRNAs and 177 species-specific miRNAs were detected by both sequencing and microarray hybridization. Our results suggest that miRNAs have wide-ranging characteristics and important roles during all stages of somatic embryogenesis in this economically important species.
The time is influenced by the transition to the operating room, the type of anesthesia and lack of information clearly stated to the team. The fetal prognosis is not limited to the "decision-to-delivery" interval but it remains essential in situations of emergency. The 15 or 30minutes interval is discussed in the literature. Obviously, the delay must be appreciated based on certain parameters (medical personnel, architecture) and each Alpha must adapt their practice to the physical working environment to meet the recommended objectives. However, the introduction of a protocol for extreme emergencies would allow for optimal responsiveness of all the teams involved and should result in a compliance period of 30minutes. Teamwork and adherence to procedures can improve these results.