Are sociologic factors major determinants of prolonged hospital stay after abdominal aneurysm repair?
Knowledge of the predictive factors that are associated with prolonged LOS should identify those patients who may require prompt and efficient discharge planning, early consultation with a home care nurse, or transfer to a convalescent facility. This approach may significantly improve the utilization of hospital resources.
Our finding that cleavage at the B site is insensitive to homology shows that effective synapsis between partners does not depend on sequence matching. Cleavage at the B' site provides the earliest positive signal for a homology-dependent switch in the lambda recombination machinery. Because this switch can occur in the absence of strand joining, the results argue against models that invoke strand ligation as the critical element of homology-sensing. Alternative mechanisms are presented that involve varieties of non-covalent strand swapping. A synthesis of the present results and other recent experiments highlights the importance of the disannealing of complementary strands and their reannealing to new partners, a process traditionally described as branch migration. The reversibility of branch migration and its bias away from mismatched combinations are proposed to be the major mechanisms of homology-sensing during lambda integration.