Does comparative RNA-Seq analysis reveal pervasive tissue-specific alternative polyadenylation in Caenorhabditis elegans intestine and muscles?
For the first time, PAT-Seq allowed us to directly study tissue specific gene expression changes in an in vivo setting and compare these changes between three somatic tissues from the same organism at single-base resolution within the same experiment. We pinpoint precise tissue-specific transcriptome rearrangements and for the first time link tissue-specific alternative polyadenylation to miRNA regulation, suggesting novel and unexplored tissue-specific post-transcriptional regulatory networks in somatic cells.
The total alkaloid fraction was able to normalize the biochemical levels which were altered due to CCl4 intoxication.