Do two structurally discrete GH7-cellobiohydrolases compete for the same cellulosic substrate fiber?
Cellobiohydrolases both with and without a CBD occur in most fungal genomes where both enzymes are secreted, and likely participate in cellulose degradation. The fact that only Cbh1 binds to the substrate and in combination with CelD exhibits strong synergy only when Cbh1 is present in excess, suggests that Cbh1 unties enough chains from cellulose fibers, thus enabling processive access of CelD.
Treatment of the SSV by endovenous laser ablation using a 1470 nm laser and a radial fibre is safe and effective. Puncturing the vein at mid-calf level causes less post-operative nerve injury without affecting the recanalisation rates.