Is genetic variability in the ACE gene region surrounding the Alu I/D polymorphism maintained by balancing selection in human populations?
Our data imply the presence of a functional polymorphism in the Alu I/D region and illustrate the contribution of evolutionary models to classic single nucleotide polymorphism-phenotype association approaches by providing information about the localization of candidate functional variants.
Treatment with LPS or oxLDL leads to diverging changes in gene expression PBMC and THP-1 cells. With respect to CNP and its receptors, data gained from THP-1 cells should be further validated using naive human peripheral blood monocytes. However, THP-1 cells can serve as a negative control for e.g. future signalling pathway studies related to oxLDL effect on CNP system in monocytes/macrophages.