Is human placental amnion a novel substrate for detecting autoantibodies in autoimmune bullous diseases by immunoblotting?
Harvesting proteins from amnion does not require epidermal-dermal separation, and a sufficient yield of desmosomal and hemidesmosomal proteins can be obtained. Therefore, amnion may be a more reliable source of substrate than skin samples for immunoblot analysis of AIBDs.
Our results elucidate the underlying mechanisms for disrupted circadian rhythm in AD.