Do glomerular filtration rate slopes have significantly improved among renal transplants in the United States?
GFR slopes and 1-year survival rates have improved in the United States independent of donor quality and immunosuppressive regimen. Findings may reflect increased skill in medical management and need further evaluation to determine whether short-term improvements translate to improved long-term survival.
Herein, we provide evidence formally establishing that gplH is essential for GPL production in M. smegmatis. Inactivation of gplH also leads to a pleiotropic phenotype likely to arise from alterations in the cell envelope due to the lack of GPLs. While genes encoding MbtH-like proteins have been shown to be needed for production of siderophores and antibiotics, our study presents the first case of one such gene proven to be required for production of a cell wall component. Furthermore, our results provide the first example of a mbtH-like gene with confirmed functional role in a member of the Mycobacterium genus. Altogether, our findings demonstrate a critical role of gplH in mycobacterial biology and advance our understanding of the genetic requirements for the biosynthesis of an important group of constituents of the mycobacterial outer membrane.