Is a threshold of 10 % for myeloperoxidase by flow cytometry valid to classify acute leukemia of ambiguous and myeloid origin?
We conclude that a 10% cut-off is a secure lower limit for cMPO expression and can be used independently from SBB expression.
Prolonged sedation may affect the transcription of fast-responding genes in tissues of fish. Two hours of sedation with isoeugenol resulted in downregulation of the Na+-K+-ATPase alpha1b gene and upregulation of the HIF1 gene in gills of Atlantic salmon. The quality of RNA extracted from tissue specimens, however, was not affected by sedation treatment. Flash-freezing of tissue specimens seems to be the preferred preservation technique, when sampling fish tissue specimens for RNA extraction.