Is 677TT genotype associated with elevated risk of methotrexate ( MTX ) toxicity in juvenile idiopathic arthritis : treatment outcome , erythrocyte concentrations of MTX and folates , and MTHFR polymorphisms?
MTHFR genotyping may have a predictive value for the risk of MTX-associated toxicity in patients with JIA. Despite the lack of therapeutic effect, nonresponders accumulated adequate concentrations of EMTX.
Major advantages of this assay over conventional methods include high-throughput format, good sensitivity, and reduced sample consumption. This method can be useful for creating comprehensive inflammatory profiles, and biomarkers can be used in the clinic to assess injury severity and to objectively grade response to therapy.