Are a ( H1N1 ) pdm09 hemagglutinin D222G and D222N variants frequently harbored by patients requiring extracorporeal membrane oxygenation and advanced respiratory assistance for severe A ( H1N1 ) pdm09 infection?
A(H1N1)pdm09 HA substitutions D222G and D222N were harbored in a significantly higher proportion by patients with acute respiratory distress for A(H1N1)pdm09 severe infection requiring ICU admission and ECMO. These data emphasize the importance of monitoring viral evolution for understanding virus-host adaptation aimed at the surveillance of strain circulation and the study of viral correlates of disease severity.
Human LMCs can substitute 3T3 cells in the expansion of LSCs using the 3-dimensional culture system.