Does data recovery and integration from public databases uncover transformation-specific transcriptional downregulation of cAMP-PKA pathway-encoding genes?
Genome-wide, large-scale "omics" experimental technologies give different, complementary perspectives on the structure and regulatory properties of complex systems. Even the relatively simple, integrated workflow presented here offers opportunities not only for filtering data noise intrinsic in high throughput data, but also to progressively extract novel information that would have remained hidden otherwise. In fact we have been able to detect a strong transcriptional repression of genes encoding proteins of cAMP/PKA pathway in cancer cells of different genetic origins. The basic workflow presented herein may be easily extended by incorporating other tools and can be applied even by researchers with poor bioinformatics skills.
This retrospective analysis suggests that the HLA B27 phenotype promotes recurrence of herpetic eye disease following penetrating keratoplasty. HLA B27-positive patients should be closely monitored, and the indications for prophylactic antiviral therapy should be viewed liberally in this group.