Does a HA2-Fusion tag limit the endosomal release of its protein cargo despite causing endosomal lysis?
These results show that E5 causes the retention of its fused protein inside endosomes even after lysis takes place.
We present the first structure of a PH domain with a strong negative overall electrostatic potential. The presence of a deep pocket lined with negative charges suggests that the domain binds to ligands other than acidic phospholipids. The abundance of this class of PH domain in the C. elegans genome suggests a prominent role in mediating protein-protein interactions.