Calcium imaging a single wavelength or two wavelengths expressed as a ratio (ratiometric indicators). The derived fluorescence intensities and ratios are plotted against calibrated values for known Ca levels to learn Ca concentration. Light field microscopy methods extend functional readout of neural activity capabilities in 3D volumes. Calcium imaging Calcium imaging is a scientific technique usually carried out in research which is designed to show the calcium (Ca) status of an isolated cell, tissue or medium. Calcium imaging techniques take advantage of so-called calcium indicators, fluorescent molecules that can respond to the binding of Ca ions by changing their fluorescence properties. Two
International breastfeeding symbol public places. These signs need to be designed carefully because they need to be understood at a glance by most people without written descriptions explaining what they mean. The International Breastfeeding Symbol was created specifically to address the perceived problem of not having a universally accepted and understood symbol for breastfeeding available in public places. The modern iconography representing infancy usually involves artificial feeding or soothing objects, like a nurser bottle icon or pacifier symbol. Nursing rooms traditionally use a baby bottle symbol to designate what they are instead of a symbol of a mother nursing a child. The International