Basketball the basketball is also regulated. For men, the official ball is in circumference (size 7, or a "295 ball") and weighs 22 oz (623.69 grams). If women are playing, the official basketball size is in circumference (size 6, or a "285 ball") with a weight of 20 oz (567 grams). In 3x3, a formalized version of the halfcourt 3-on-3 game, a dedicated ball with the circumference of a size 6 ball but the weight of a size 7 ball is used in all competitions (men's, women's, and mixed teams). The ball may be advanced toward the basket by being shot,
Molecular-weight size marker Southern Blot analysis was developed in 1990, providing the first marker to combine target DNA and probe DNA. This technique took advantage of logarithmic spacing, and could be used to identify target bands ranging over a length of 20,000 nucleotides. There are two common methods in which to construct a DNA molecular-weight size marker. One such method employs the technique of partial ligation. DNA ligation is the process by which linear DNA pieces are connected to each other via covalent bonds; more specifically, these bonds are phosphodiester bonds. Here, a 100bp duplex DNA piece is partially ligated. The consequence of