Calcium imaging a single wavelength or two wavelengths expressed as a ratio (ratiometric indicators). The derived fluorescence intensities and ratios are plotted against calibrated values for known Ca levels to learn Ca concentration. Light field microscopy methods extend functional readout of neural activity capabilities in 3D volumes. Calcium imaging Calcium imaging is a scientific technique usually carried out in research which is designed to show the calcium (Ca) status of an isolated cell, tissue or medium. Calcium imaging techniques take advantage of so-called calcium indicators, fluorescent molecules that can respond to the binding of Ca ions by changing their fluorescence properties. Two
Symbol cues, body language, sound, and other contextual clues. Semiotics is linked with both linguistics and psychology. Semioticians thus not only study what a symbol implies, but also how it got its meaning and how it functions to make meaning in society. Symbols allow the human brain continuously to create meaning using sensory input and decode symbols through both denotation and connotation. Swiss psychoanalyst Carl Jung, who studied archetypes, proposed an alternative definition of symbol, distinguishing it from the term "sign". In Jung's view, a sign stands for something known, as a word stands for its referent. He contrasted this with