Fractionation of carbon isotopes in oxygenic photosynthesis characterization of food chains. Oxygenic photosynthesis is a metabolic pathway facilitated by autotrophs, including plants, algae, and cyanobacteria. This pathway converts inorganic carbon dioxide from the atmosphere or aquatic environment into carbohydrates, using water and energy from light, then releases molecular oxygen as a product. Organic carbon contains less of the stable isotope Carbon-13, or C, relative to the initial inorganic carbon from the atmosphere or water because photosynthetic carbon fixation involves several fractionating reactions with kinetic isotope effects. These reactions undergo a kinetic isotope effect because they are limited by overcoming an activation energy barrier. The lighter isotope has
Carbon dioxide stretching mode at 1388 cm which is only observed in the Raman spectrum. Carbon dioxide is soluble in water, in which it reversibly forms (carbonic acid), which is a weak acid since its ionization in water is incomplete. The hydration equilibrium constant of carbonic acid is formula_1 (at 25 °C). Hence, the majority of the carbon dioxide is not converted into carbonic acid, but remains as molecules, not affecting the pH. The relative concentrations of , and the deprotonated forms (bicarbonate) and (carbonate) depend on the pH. As shown in a Bjerrum plot, in neutral or slightly alkaline water (pH