Calcium imaging a single wavelength or two wavelengths expressed as a ratio (ratiometric indicators). The derived fluorescence intensities and ratios are plotted against calibrated values for known Ca levels to learn Ca concentration. Light field microscopy methods extend functional readout of neural activity capabilities in 3D volumes. Calcium imaging Calcium imaging is a scientific technique usually carried out in research which is designed to show the calcium (Ca) status of an isolated cell, tissue or medium. Calcium imaging techniques take advantage of so-called calcium indicators, fluorescent molecules that can respond to the binding of Ca ions by changing their fluorescence properties. Two
Answering the Question: What is Enlightenment? II of Prussia for creating these preconditions. Kant focused on religious issues, saying that "our rulers" had less interest in telling citizens what to think in regard to artistic and scientific issues. Kant answers the question in the first sentence of the essay: "Enlightenment is man's emergence from his self-incurred immaturity (Unmündigkeit)." He argues that the immaturity is self-inflicted not from a lack of understanding, but from the lack of courage to use one's reason, intellect, and wisdom without the guidance of another. He exclaims that the motto of enlightenment is "Sapere aude"! – Dare to be wise! In the