Calcium imaging a single wavelength or two wavelengths expressed as a ratio (ratiometric indicators). The derived fluorescence intensities and ratios are plotted against calibrated values for known Ca levels to learn Ca concentration. Light field microscopy methods extend functional readout of neural activity capabilities in 3D volumes. Calcium imaging Calcium imaging is a scientific technique usually carried out in research which is designed to show the calcium (Ca) status of an isolated cell, tissue or medium. Calcium imaging techniques take advantage of so-called calcium indicators, fluorescent molecules that can respond to the binding of Ca ions by changing their fluorescence properties. Two
Calcium carbonate Calcium carbonate shares the typical properties of other carbonates. Notably, Calcium carbonate will react with water that is saturated with carbon dioxide to form the soluble calcium bicarbonate. This reaction is important in the erosion of carbonate rock, forming caverns, and leads to hard water in many regions. An unusual form of calcium carbonate is the hexahydrate, ikaite, CaCO·6HO. Ikaite is stable only below 8 °C. The vast majority of calcium carbonate used in industry is extracted by mining or quarrying. Pure calcium carbonate (e.g. for food or pharmaceutical use), can be produced from a pure quarried source (usually marble).