Basketball the basketball is also regulated. For men, the official ball is in circumference (size 7, or a "295 ball") and weighs 22 oz (623.69 grams). If women are playing, the official basketball size is in circumference (size 6, or a "285 ball") with a weight of 20 oz (567 grams). In 3x3, a formalized version of the halfcourt 3-on-3 game, a dedicated ball with the circumference of a size 6 ball but the weight of a size 7 ball is used in all competitions (men's, women's, and mixed teams). The ball may be advanced toward the basket by being shot,
Molecular-weight size marker separating based on size, the ideal method is SDS-PAGE or polyacrylamide gel electrophoresis and molecular-weight size markers are the appropriate standards to use. Gels can vary in size. The number of samples to be run will determine the appropriate gel size. All gels are divided into lanes that run parallel through the gel. Each lane will contain a specific sample. Typically, molecular-weight size standards are placed in an outer lane. If a gel has a particularly high number of lanes, then multiple ladders may be placed across the gel for higher clarity. Proteins and standards are pipetted on the gel