Calcium imaging Calcium imaging Calcium imaging is a scientific technique usually carried out in research which is designed to show the calcium (Ca) status of an isolated cell, tissue or medium. Calcium imaging techniques take advantage of so-called calcium indicators, fluorescent molecules that can respond to the binding of Ca ions by changing their fluorescence properties. Two main classes of calcium indicators exist: chemical indicators and genetically encoded calcium indicators (GECI). Calcium imaging can be used to optically probe intracellular calcium in living animals. This technique has allowed studies of calcium signalling in a wide variety of cell types and neuronal activity
Answering the Question: What is Enlightenment? II of Prussia for creating these preconditions. Kant focused on religious issues, saying that "our rulers" had less interest in telling citizens what to think in regard to artistic and scientific issues. Kant answers the question in the first sentence of the essay: "Enlightenment is man's emergence from his self-incurred immaturity (Unmündigkeit)." He argues that the immaturity is self-inflicted not from a lack of understanding, but from the lack of courage to use one's reason, intellect, and wisdom without the guidance of another. He exclaims that the motto of enlightenment is "Sapere aude"! – Dare to be wise! In the