Calcium looping example, adding Ca-looping to all coal-fired plants in the UK would generate enough waste for 33 Mtonnes/yr of cement production, whereas current cement production in the UK is only 12.5 Mtonnes/yr. Hence, if the technology is applied on a large scale, the purge rate of CaO should be optimized to minimize waste. To fully gauge the viability of calcium looping as a capture process, it is necessary to consider the political, environmental, and health effects of the process as well. Though many recent scientific reports (e.g.: the seven-wedge stabilization plan by Pacala and Socolow) convey an urgent need to deploy
Calcium imaging dye in all cells or selectively in certain cellular subtypes. GECIs have been used in the studies of neuron, T-cell, cardiomyocyte, etc. Regardless of the type of indicator used the imaging procedure is generally very similar. Isolated cells loaded with an indicator or expressing it in the case of GECI, can be viewed using a fluorescence microscope and captured by a Scientific CMOS (sCMOS) camera or CCD camera. Confocal and two-photon microscopes provide improved sectioning ability so that calcium signals can be resolved in micro domains or (for example) synaptic boutons. Images are analyzed by measuring fluorescence intensity changes for