Liver function tests protein synthesized in the liver. It is the carrier of the copper ion. Its levels is increased in infections, rheumatoid arthritis, pregnancy, non Wilson liver disease and obstructive jaundice. In Wilson disease, the ceruloplasmin level is depressed which lead to copper accumulation in body tissues. AFP is significantly expressed in foetal liver. However, the mechanism that led to the suppression of AFP synthesis in adults is not fully known. Exposure of the liver to cancer-causing agents and arrest of liver maturation in childhood can lead to the rise in AFP. AFP can reach until 400–500 μg/L in hepatocellular carcinoma. AFP
Lead and Copper Rule necessarily representative of the lead corrosion potential in a particular water/pipe system, nor did they measure the amount of lead to which customers were exposed. In the second half of the twentieth century, concern was growing about what constituted a safe level of lead. The 1986 Safe Drinking Water Act amendments defined "lead-free" plumbing and prohibited the use of plumbing for public water supply that did not meet the new definition. The amendments also required EPA to set standards limiting the concentration of lead in public water systems. EPA first issued the Lead and Copper Rule on June 7, 1991.