Calcium imaging a single wavelength or two wavelengths expressed as a ratio (ratiometric indicators). The derived fluorescence intensities and ratios are plotted against calibrated values for known Ca levels to learn Ca concentration. Light field microscopy methods extend functional readout of neural activity capabilities in 3D volumes. Calcium imaging Calcium imaging is a scientific technique usually carried out in research which is designed to show the calcium (Ca) status of an isolated cell, tissue or medium. Calcium imaging techniques take advantage of so-called calcium indicators, fluorescent molecules that can respond to the binding of Ca ions by changing their fluorescence properties. Two
Calcium citrate Calcium citrate Calcium citrate is the calcium salt of citric acid. It is commonly used as a food additive (E333), usually as a preservative, but sometimes for flavor. In this sense, it is similar to sodium citrate. Calcium citrate is also found in some dietary calcium supplements (e.g. Citracal). Calcium makes up 24.1% of calcium citrate (anhydrous) and 21.1% of calcium citrate (tetrahydrate) by mass. The tetrahydrate occurs in nature as the mineral Earlandite. Calcium citrate is sparingly soluble in water. Needle-shaped crystals of tricalcium dicitrate tetrahydrate [Ca(CHO)(HO)]·2HO were obtained by hydrothermal synthesis. The crystal structure comprises a three-dimensional network