Bt brinjal from non-transgenic, and "aad" is used to identify the transformed bacteria used during the development of the construct. "Aad" contains a bacterial promoter and is not expressed in the Bt brinjal. The completed construct was inserted into young cotyledons from the brinjal plants using an "Agrobacterium"-mediated technique. "Agrobacterium" naturally inserts DNA into plants from its Ti plasmid, and scientists use this to insert genes of interest into various plants. The transformed plants were regenerated and analyzed for the presence of the gene through Southern blotting. The plants' progeny were also analyzed to identify lines segregating in a Mendelian fashion. When
Forbidden fruit Although commonly confused with a seed, in the study of botany a wheat berry is technically a simple fruit known as a caryopsis, which has the same structure as an apple. Just as an apple is a fleshy fruit that contains seeds, a grain is a dry fruit that absorbs water and contains a seed. The confusion comes from the fact that the fruit of a grass happens to have a form similar to some seeds. A fresco in the 13th-century Plaincourault Abbey in France depicts Adam and Eve in the Garden of Eden, flanking a Tree of Knowledge that