Bt brinjal from non-transgenic, and "aad" is used to identify the transformed bacteria used during the development of the construct. "Aad" contains a bacterial promoter and is not expressed in the Bt brinjal. The completed construct was inserted into young cotyledons from the brinjal plants using an "Agrobacterium"-mediated technique. "Agrobacterium" naturally inserts DNA into plants from its Ti plasmid, and scientists use this to insert genes of interest into various plants. The transformed plants were regenerated and analyzed for the presence of the gene through Southern blotting. The plants' progeny were also analyzed to identify lines segregating in a Mendelian fashion. When
The Vegetable, or From President to Postman The Vegetable, or From President to Postman The Vegetable, or From President to Postman is a short story by F. Scott Fitzgerald that he developed into a play. In the original publication of "The Vegetable, or From President to Postman" (1923), F. Scott Fitzgerald included the following quotation on the title page: “Any man who doesn’t want to get on in the world, to make a million dollars, and maybe even park his toothbrush in the White House, hasn’t got as much to him as a good dog has—he’s nothing more or less than a vegetable.” Fitzgerald used this quotation,