Bt brinjal from non-transgenic, and "aad" is used to identify the transformed bacteria used during the development of the construct. "Aad" contains a bacterial promoter and is not expressed in the Bt brinjal. The completed construct was inserted into young cotyledons from the brinjal plants using an "Agrobacterium"-mediated technique. "Agrobacterium" naturally inserts DNA into plants from its Ti plasmid, and scientists use this to insert genes of interest into various plants. The transformed plants were regenerated and analyzed for the presence of the gene through Southern blotting. The plants' progeny were also analyzed to identify lines segregating in a Mendelian fashion. When
Fresh Fruit and Vegetable Program largely lauded, there are a few criticisms and controversies. The biggest one is a criticism of the program's dedication to fresh produce, instead of also permitting other forms of produce – such as canned, frozen, or dried – which are frequently cheaper. There is another program named the Fresh Fruit and Vegetable program that is funded by the United States Department of Defense. The DoD's FFVP was created in 1996 to help schools procure more fresh fruits and vegetables for their students. The program serves as a go-between for schools and fresh produce vendors, allowing schools to order food directly