Bt brinjal from non-transgenic, and "aad" is used to identify the transformed bacteria used during the development of the construct. "Aad" contains a bacterial promoter and is not expressed in the Bt brinjal. The completed construct was inserted into young cotyledons from the brinjal plants using an "Agrobacterium"-mediated technique. "Agrobacterium" naturally inserts DNA into plants from its Ti plasmid, and scientists use this to insert genes of interest into various plants. The transformed plants were regenerated and analyzed for the presence of the gene through Southern blotting. The plants' progeny were also analyzed to identify lines segregating in a Mendelian fashion. When
The Fruit is Ripe with whom she tours the Aegean. Tom leaves her after a fight, but eventually comes back to her. The Fruit is Ripe The Fruit Is Ripe ( or "Greek Figs") is a 1977 German softcore erotic comedy film directed by Sigi Rothemund. Patricia (Betty Vergès) is a young woman from a wealthy family in Greece who is bound to Munich for college. At the airport, she gives away her ticket and instead begins to hitchhike around Greece by automobile and boat in a journey of self-discovery. In the course of her travels, she becomes an object of desire. She's pursued