Bt brinjal from non-transgenic, and "aad" is used to identify the transformed bacteria used during the development of the construct. "Aad" contains a bacterial promoter and is not expressed in the Bt brinjal. The completed construct was inserted into young cotyledons from the brinjal plants using an "Agrobacterium"-mediated technique. "Agrobacterium" naturally inserts DNA into plants from its Ti plasmid, and scientists use this to insert genes of interest into various plants. The transformed plants were regenerated and analyzed for the presence of the gene through Southern blotting. The plants' progeny were also analyzed to identify lines segregating in a Mendelian fashion. When
Marrow (vegetable) processed for food when required. They are a popular vegetable in Great Britain and areas with significant British influence, though their popularity is waning in favor of immature summer squash like courgette. Giant marrows are grown competitively in the United Kingdom where the term "marrow" is often restricted to the striped, thick-skinned cultivar. In a culinary context, marrows are treated as a vegetable; usually cooked and presented as a savory dish or accompaniment. Botanically, marrows are fruit, a type of botanical berry, being the swollen ovary of the marrow flower. Marrows, like all squash, have their ancestry in the Americas.