Bt brinjal from non-transgenic, and "aad" is used to identify the transformed bacteria used during the development of the construct. "Aad" contains a bacterial promoter and is not expressed in the Bt brinjal. The completed construct was inserted into young cotyledons from the brinjal plants using an "Agrobacterium"-mediated technique. "Agrobacterium" naturally inserts DNA into plants from its Ti plasmid, and scientists use this to insert genes of interest into various plants. The transformed plants were regenerated and analyzed for the presence of the gene through Southern blotting. The plants' progeny were also analyzed to identify lines segregating in a Mendelian fashion. When
Unusually shaped vegetable Unusually shaped vegetable An unusually shaped vegetable is a vegetable or fruit that has grown into a shape not in line with its normal body plan. While some examples are just oddly shaped, others are heralded for their amusing appearance, often because they resemble a body part such as the buttocks or genitalia. Pareidolia can be common in vegetables, with some people reporting the appearance of religious imagery. Vegetables usually grow into an unusual shape due to environmental conditions. Damage to one part of the vegetable can cause the growth to slow in that area while the rest grows at