Bt brinjal from non-transgenic, and "aad" is used to identify the transformed bacteria used during the development of the construct. "Aad" contains a bacterial promoter and is not expressed in the Bt brinjal. The completed construct was inserted into young cotyledons from the brinjal plants using an "Agrobacterium"-mediated technique. "Agrobacterium" naturally inserts DNA into plants from its Ti plasmid, and scientists use this to insert genes of interest into various plants. The transformed plants were regenerated and analyzed for the presence of the gene through Southern blotting. The plants' progeny were also analyzed to identify lines segregating in a Mendelian fashion. When
Berry 1.8 million acres of land worldwide cultivating berries, with 6.3 million tons produced. According to figures from Global Berry Congress 2015 in the US, over 6 million dollars of soft fruit are sold, accounting for 19% of total supermarket revenue, more than bananas (a botanical berry) at 11%, and apples (14%), with continued rapid growth in the market expected. In certain regions, berrypicking can be a large part of the economy, and it is becoming increasingly common for western European countries such as Sweden and Finland to import cheap labor from Thailand or Bulgaria to do the berry picking. This