Theobroma cacao as culinary, purposes. Some mixtures included maize, chili, vanilla ("Vanilla planifolia"), and honey. Archaeological evidence for use of cacao, while relatively sparse, has come from the recovery of whole cacao beans at Uaxactun, Guatemala and from the preservation of wood fragments of the cacao tree at Belize sites including Cuello and Pulltrouser Swamp. In addition, analysis of residues from ceramic vessels has found traces of theobromine and caffeine in early formative vessels from Puerto Escondido, Honduras (1100–900 BC) and in middle formative vessels from Colha, Belize (600–400 BC) using similar techniques to those used to extract chocolate residues from four
Cacao necrosis virus virus is not infective in sap after heating to 65 °C for 10 minutes, dilution to 0.0001 virions, or storage for 7 days at room temperature. Cacao necrosis virus "Cacao necrosis virus" (CNV) is a plant pathogenic virus of the genus "nepovirus" that infects "Theobroma cacao" en natura causing cacao necrosis disease. CNV is considered synonymous with Strain S of cacao swollen shoot virus. Unlike cacao swollen-shoot virus, it is not transmitted by mealybugs nor vectored by aphids, beetles or leafhoppers that also commonly infest cacao. It is serologically, distantly related to Tomato black ring virus and very distantly related