Bt brinjal from non-transgenic, and "aad" is used to identify the transformed bacteria used during the development of the construct. "Aad" contains a bacterial promoter and is not expressed in the Bt brinjal. The completed construct was inserted into young cotyledons from the brinjal plants using an "Agrobacterium"-mediated technique. "Agrobacterium" naturally inserts DNA into plants from its Ti plasmid, and scientists use this to insert genes of interest into various plants. The transformed plants were regenerated and analyzed for the presence of the gene through Southern blotting. The plants' progeny were also analyzed to identify lines segregating in a Mendelian fashion. When
International Fruit and Vegetable Juice Association International Fruit and Vegetable Juice Association The International Fruit and Vegetable Juice Association (IFU) represents the worldwide juice sector interests as the registered non-governmental organization (NGO) at Codex Alimentarius. It also represents the sector with World Health Organization (WHO), World Trade Organization (WTO), World Customs Organization (WCO) and any other relevant governmental or intergovernmental organisation. The IFU was founded in 1949 as the International Federation of Fruit Juice Producers named International Fruit Juice Union. The name of the organisation was changed in 2015 in International Fruit an Vegetable Juice Association to reflect the diversity of the membership base. Today it