Bt brinjal from non-transgenic, and "aad" is used to identify the transformed bacteria used during the development of the construct. "Aad" contains a bacterial promoter and is not expressed in the Bt brinjal. The completed construct was inserted into young cotyledons from the brinjal plants using an "Agrobacterium"-mediated technique. "Agrobacterium" naturally inserts DNA into plants from its Ti plasmid, and scientists use this to insert genes of interest into various plants. The transformed plants were regenerated and analyzed for the presence of the gene through Southern blotting. The plants' progeny were also analyzed to identify lines segregating in a Mendelian fashion. When
Fresh Fruit and Vegetable Program snacks outside normal mealtime hours as dictated by the National School Lunch Program (NSLP) and School Breakfast Program. There are limitations on how produce can be prepared. Dip must be low- or non-fat, and cooked vegetables are to be limited to once a week. It is recommended to use pre-cut fruit and vegetable trays to ease storage and serving. , canned, frozen, and dried produce are not reimbursed under the FFVP, although there is a bill, Improving Child Nutrition and Education Act of 2016, with a provision that increases the FFVP's scope to include canned, frozen, and dried produce. The