Bt brinjal from non-transgenic, and "aad" is used to identify the transformed bacteria used during the development of the construct. "Aad" contains a bacterial promoter and is not expressed in the Bt brinjal. The completed construct was inserted into young cotyledons from the brinjal plants using an "Agrobacterium"-mediated technique. "Agrobacterium" naturally inserts DNA into plants from its Ti plasmid, and scientists use this to insert genes of interest into various plants. The transformed plants were regenerated and analyzed for the presence of the gene through Southern blotting. The plants' progeny were also analyzed to identify lines segregating in a Mendelian fashion. When
Fruit anatomy like the berry most of the pericarp is fleshy but the endocarp is cartilaginous, an apple is an example of a pome. Lastly, drupes are known for being one seeded with a fleshy mesocarp, an example of this would be the peach. However, there are fruits were the fleshy portion is developed from tissues that are not the ovary, such as in the strawberry. The edible part of the strawberry is formed from the receptacle of the flower. Due, to this difference the strawberry is known as a false fruit or an accessory fruit. There is a shared method of