Bt brinjal from non-transgenic, and "aad" is used to identify the transformed bacteria used during the development of the construct. "Aad" contains a bacterial promoter and is not expressed in the Bt brinjal. The completed construct was inserted into young cotyledons from the brinjal plants using an "Agrobacterium"-mediated technique. "Agrobacterium" naturally inserts DNA into plants from its Ti plasmid, and scientists use this to insert genes of interest into various plants. The transformed plants were regenerated and analyzed for the presence of the gene through Southern blotting. The plants' progeny were also analyzed to identify lines segregating in a Mendelian fashion. When
Fruit and vegetable wash Fruit and vegetable wash A vegetable wash is a cleaning product designed to aid in the removal process of dirt, wax and pesticides from fruit and vegetables before they are consumed. All fresh produce, even organic, can harbor residual pesticides, dirt or harmful microorganisms on the surface. Vegetable washes may either be a number specially-marketed commercial brands, or they may be home recipes. Commercial vegetable washes generally contain surfactants, along with chelating agents, antioxidants, and other agents. Home recipes are generally dilutions of hydrogen peroxide or vinegar, the former of which may be dangerous at high concentrations. Neither the U.S.