Bt brinjal from non-transgenic, and "aad" is used to identify the transformed bacteria used during the development of the construct. "Aad" contains a bacterial promoter and is not expressed in the Bt brinjal. The completed construct was inserted into young cotyledons from the brinjal plants using an "Agrobacterium"-mediated technique. "Agrobacterium" naturally inserts DNA into plants from its Ti plasmid, and scientists use this to insert genes of interest into various plants. The transformed plants were regenerated and analyzed for the presence of the gene through Southern blotting. The plants' progeny were also analyzed to identify lines segregating in a Mendelian fashion. When
Fresh Fruit and Vegetable Program the FFVP have been positive, with a slight increase in participating students' average fruit and vegetable consumption without a corresponding increase in daily calories. Students also had a more positive view towards fruits and vegetables during the program, and 91% teachers and other school administrators have also reported favorable opinions towards the program. Teachers observed that students are more able to focus in the evenings and aren't as hungry at the end of the day. The FFVP also helps reduce childhood obesity and is a more cost-effective method than other efforts to reduce childhood obesity. Although the FFVP has been