Bt brinjal from non-transgenic, and "aad" is used to identify the transformed bacteria used during the development of the construct. "Aad" contains a bacterial promoter and is not expressed in the Bt brinjal. The completed construct was inserted into young cotyledons from the brinjal plants using an "Agrobacterium"-mediated technique. "Agrobacterium" naturally inserts DNA into plants from its Ti plasmid, and scientists use this to insert genes of interest into various plants. The transformed plants were regenerated and analyzed for the presence of the gene through Southern blotting. The plants' progeny were also analyzed to identify lines segregating in a Mendelian fashion. When
Horticulture Netting or Vegetable Support Net Horticulture Netting or Vegetable Support Net Horticulture Netting or Vegetable Support Net Starting in the 1960s, in Europe a new rigid mesh and net product made out of polypropylene started being used. The most famous factories were located in England and Italy, given the fact that these two countries where the most advanced in machine tooling and extrusion, then with the passing of time this technology found its way into other parts of the world, and today it is very easy to find who manufactures this type of base product anywhere in the world Once the semi-finished extruded product is