Bt brinjal from non-transgenic, and "aad" is used to identify the transformed bacteria used during the development of the construct. "Aad" contains a bacterial promoter and is not expressed in the Bt brinjal. The completed construct was inserted into young cotyledons from the brinjal plants using an "Agrobacterium"-mediated technique. "Agrobacterium" naturally inserts DNA into plants from its Ti plasmid, and scientists use this to insert genes of interest into various plants. The transformed plants were regenerated and analyzed for the presence of the gene through Southern blotting. The plants' progeny were also analyzed to identify lines segregating in a Mendelian fashion. When
Vegetable Lamb of Tartary that sleeps by day And wakes at night, though rooted in the ground, To feed on grass within its reach around.</poem> Vegetable Lamb of Tartary The Vegetable Lamb of Tartary (Latin: "Agnus scythicus" or "Planta Tartarica Barometz") is a legendary zoophyte of Central Asia, once believed to grow sheep as its fruit. It was believed the sheep were connected to the plant by an umbilical cord and grazed the land around the plant. When all accessible foliage was gone, both the plant and sheep died. Underlying the legend is the cotton plant, which was unknown in Northern Europe before the