Bt brinjal from non-transgenic, and "aad" is used to identify the transformed bacteria used during the development of the construct. "Aad" contains a bacterial promoter and is not expressed in the Bt brinjal. The completed construct was inserted into young cotyledons from the brinjal plants using an "Agrobacterium"-mediated technique. "Agrobacterium" naturally inserts DNA into plants from its Ti plasmid, and scientists use this to insert genes of interest into various plants. The transformed plants were regenerated and analyzed for the presence of the gene through Southern blotting. The plants' progeny were also analyzed to identify lines segregating in a Mendelian fashion. When
Fruit preserves can be easily verified by searching the listings under "fruit spread" on common web sites, such as those of Amazon or Walmart, or to look at the ingredient list and nutritional information on specific fruit spread products. Jam typically contains both the juice and flesh of a fruit or vegetable, although one cookbook defines it as a cooked and jelled puree. The term "jam" refers to a product made of whole fruit cut into pieces or crushed, then heated with water and sugar to activate its pectin before being put into containers: Pectin is mainly D-galacturonic acid connected by α