Bt brinjal from non-transgenic, and "aad" is used to identify the transformed bacteria used during the development of the construct. "Aad" contains a bacterial promoter and is not expressed in the Bt brinjal. The completed construct was inserted into young cotyledons from the brinjal plants using an "Agrobacterium"-mediated technique. "Agrobacterium" naturally inserts DNA into plants from its Ti plasmid, and scientists use this to insert genes of interest into various plants. The transformed plants were regenerated and analyzed for the presence of the gene through Southern blotting. The plants' progeny were also analyzed to identify lines segregating in a Mendelian fashion. When
Berry (botany) Some sources have attempted to quantify the difference, e.g. requiring the endocarp to be less than 2 mm thick in a berry. Examples of botanical berries include: "True berries", or "baccae", may also be required to have a thin outer skin, not self-supporting when removed from the berry. This distinguishes, for example, a "Vaccinium" or "Solanum" berry from an "Adansonia" (baobab) amphisarca, which has a dry, more rigid and self-supporting skin. The fruit of citrus, such as the orange, kumquat and lemon, is a berry with a thick rind and a very juicy interior divided into segments by septa, that