Bt brinjal from non-transgenic, and "aad" is used to identify the transformed bacteria used during the development of the construct. "Aad" contains a bacterial promoter and is not expressed in the Bt brinjal. The completed construct was inserted into young cotyledons from the brinjal plants using an "Agrobacterium"-mediated technique. "Agrobacterium" naturally inserts DNA into plants from its Ti plasmid, and scientists use this to insert genes of interest into various plants. The transformed plants were regenerated and analyzed for the presence of the gene through Southern blotting. The plants' progeny were also analyzed to identify lines segregating in a Mendelian fashion. When
Berry 1800s, English breeders of strawberry made varieties of "F. ananassa" which were important in strawberry breeding in Europe, and hundreds of cultivars have since been produced through the breeding of strawberries. A form of the word "berry" is found in all the Germanic languages; for example, Old English "berie" compares with Old Saxon and Old High German "beri", and Old Norse "ber". These forms point to the Proto Germanic "*bazją". In Old English, the word was mainly applied to grapes, but has since grown to its current definition. In botanical terminology, a berry is a simple fruit with seeds and