Bt brinjal from non-transgenic, and "aad" is used to identify the transformed bacteria used during the development of the construct. "Aad" contains a bacterial promoter and is not expressed in the Bt brinjal. The completed construct was inserted into young cotyledons from the brinjal plants using an "Agrobacterium"-mediated technique. "Agrobacterium" naturally inserts DNA into plants from its Ti plasmid, and scientists use this to insert genes of interest into various plants. The transformed plants were regenerated and analyzed for the presence of the gene through Southern blotting. The plants' progeny were also analyzed to identify lines segregating in a Mendelian fashion. When
Marrow (vegetable) which is not destroyed by cooking. In August 2015, a 79-year-old German man and his wife ate a marrow grown by a neighbour. The couple noted the unusually bitter taste. Shortly afterwards they were both admitted to Heidenheim hospital, apparently with symptoms of a gastrointestinal infection. The woman, who had eaten a smaller portion, survived, while the man died. Toxicological analysis of the meal confirmed the presence of cucurbitacin. Marrow (vegetable) A marrow is a vegetable, the mature fruit of certain "Cucurbita pepo" cultivars. The immature fruit of the same or similar cultivars is called "courgette" (in Britain, Ireland, France,