Bt brinjal from non-transgenic, and "aad" is used to identify the transformed bacteria used during the development of the construct. "Aad" contains a bacterial promoter and is not expressed in the Bt brinjal. The completed construct was inserted into young cotyledons from the brinjal plants using an "Agrobacterium"-mediated technique. "Agrobacterium" naturally inserts DNA into plants from its Ti plasmid, and scientists use this to insert genes of interest into various plants. The transformed plants were regenerated and analyzed for the presence of the gene through Southern blotting. The plants' progeny were also analyzed to identify lines segregating in a Mendelian fashion. When
Fruit preserves spread made from sweetened fruit (or vegetable) juice—thus differing from jam by excluding the fruit's flesh—and is set by using its naturally occurring pectin, whereas outside North America jelly more often refers to a gelatin-based dessert, though the term is also used to refer to clear jams such as blackcurrant and apple. In the United Kingdom, redcurrant jelly is a condiment often served with lamb, game meat including venison, turkey and goose in a festive or Sunday roast. It is a clear jam, set with pectin from the fruit, and is made in the same way, by adding the redcurrants