Theobroma cacao by bees (Hymenoptera) or butterflies/moths (Lepidoptera), cacao flowers are pollinated by tiny flies, "Forcipomyia" midges in the subfamily Forcipomyiinae. Having the natural pollinator "Forcipomyia" midges for "Theobroma cacao" was shown to have more fruit production than using artificial pollinators. The fruit, called a cacao pod, is ovoid, long and wide, ripening yellow to orange, and weighs about when ripe. The pod contains 20 to 60 seeds, usually called "beans", embedded in a white pulp. The seeds are the main ingredient of chocolate, while the pulp is used in some countries to prepare refreshing juice, smoothies, jelly, and nata. Usually discarded
Theobroma cacao there were two foci for domestication, one in the Lacandon Jungle area of Mexico and another in lowland South America. More recent studies of patterns of DNA diversity, however, suggest that this is not the case. One study sampled 1241 trees and classified them into 10 distinct genetic clusters. This study also identified areas, for example around Iquitos in modern Peru and Ecuador, where representatives of several genetic clusters originated more than 5000 years ago, leading to development of the variety, Nacional cocoa bean. This result suggests that this is where "T. cacao" was originally domesticated, probably for the pulp