Bt brinjal from non-transgenic, and "aad" is used to identify the transformed bacteria used during the development of the construct. "Aad" contains a bacterial promoter and is not expressed in the Bt brinjal. The completed construct was inserted into young cotyledons from the brinjal plants using an "Agrobacterium"-mediated technique. "Agrobacterium" naturally inserts DNA into plants from its Ti plasmid, and scientists use this to insert genes of interest into various plants. The transformed plants were regenerated and analyzed for the presence of the gene through Southern blotting. The plants' progeny were also analyzed to identify lines segregating in a Mendelian fashion. When
International Fruit and Vegetable Juice Association as an NGO at government and international organisations. It acts as a regulatory information centre and supports harmonisation of international regulatory standards and practices across the juice sector. The Methods of Analysis Commission, chaired by Mikko Hofsommer of GfL in Berlin, has as its primary mission to create and standardise methods of analysis, specifically for processed fruit and vegetable products. Most of the published IFU methods are listed as official methods in codex. The Microbiology working group, chaired by Andreas Politzer and Rudolph Wild is a subgroup of the methods of analysis commission, focuses on similar issues within their field.