Bt brinjal from non-transgenic, and "aad" is used to identify the transformed bacteria used during the development of the construct. "Aad" contains a bacterial promoter and is not expressed in the Bt brinjal. The completed construct was inserted into young cotyledons from the brinjal plants using an "Agrobacterium"-mediated technique. "Agrobacterium" naturally inserts DNA into plants from its Ti plasmid, and scientists use this to insert genes of interest into various plants. The transformed plants were regenerated and analyzed for the presence of the gene through Southern blotting. The plants' progeny were also analyzed to identify lines segregating in a Mendelian fashion. When
Vegetable box scheme to week as selected by the box scheme provider on the basis of seasonality and availability. Some schemes offer the option of purchasing extra goods to be delivered along with the vegetable box, such as dairy produce and meat. Vegetable box scheme A vegetable box scheme is an operation that delivers fresh fruit and vegetables, often locally grown and organic, either directly to the customer or to a local collection point. Typically the produce is sold as an ongoing weekly subscription and the offering may vary week to week depending on what is in season. The first organic box scheme