Bt brinjal from non-transgenic, and "aad" is used to identify the transformed bacteria used during the development of the construct. "Aad" contains a bacterial promoter and is not expressed in the Bt brinjal. The completed construct was inserted into young cotyledons from the brinjal plants using an "Agrobacterium"-mediated technique. "Agrobacterium" naturally inserts DNA into plants from its Ti plasmid, and scientists use this to insert genes of interest into various plants. The transformed plants were regenerated and analyzed for the presence of the gene through Southern blotting. The plants' progeny were also analyzed to identify lines segregating in a Mendelian fashion. When
Vegetable juice be carefully considered. Some vegetables such as beets also contain large amounts of sugar, so care must be taken when adding these to juices. Although the actual nutritional benefits of vegetable juice are contested, a recent UC Davis study found that drinking vegetable juice daily significantly increased drinkers' chances of meeting the daily recommended number of vegetable servings. Having an easy source of vegetables encouraged drinkers to incorporate more vegetables into their diets. Children who refuse whole vegetables may find vegetable juice, when blended with fruit juice, a palatable alternative. Vegetable juice Vegetable juice is a juice drink made primarily