Bt brinjal from non-transgenic, and "aad" is used to identify the transformed bacteria used during the development of the construct. "Aad" contains a bacterial promoter and is not expressed in the Bt brinjal. The completed construct was inserted into young cotyledons from the brinjal plants using an "Agrobacterium"-mediated technique. "Agrobacterium" naturally inserts DNA into plants from its Ti plasmid, and scientists use this to insert genes of interest into various plants. The transformed plants were regenerated and analyzed for the presence of the gene through Southern blotting. The plants' progeny were also analyzed to identify lines segregating in a Mendelian fashion. When
Vegetable Lamb of Tartary of the Irish Sea with gourd-like fruits that fell into the water and became birds called Bernacles. He is referring to the legendary plant-animal, the barnacle tree, which was believed to drop its ripened fruit into the sea near the Orkney Islands. The ripened fruit would then release "barnacle geese" that would live in the water, growing to mature geese. The alleged existence of this fellow plant-animal was accepted as an explanation for migrating geese from the North. In his work "The Shui-yang or Watersheep and The Agnus Scythicus or Vegetable Lamb" (1892), Gustav Schlegel points to Chinese legends of