Bt brinjal from non-transgenic, and "aad" is used to identify the transformed bacteria used during the development of the construct. "Aad" contains a bacterial promoter and is not expressed in the Bt brinjal. The completed construct was inserted into young cotyledons from the brinjal plants using an "Agrobacterium"-mediated technique. "Agrobacterium" naturally inserts DNA into plants from its Ti plasmid, and scientists use this to insert genes of interest into various plants. The transformed plants were regenerated and analyzed for the presence of the gene through Southern blotting. The plants' progeny were also analyzed to identify lines segregating in a Mendelian fashion. When
Fruit in which part or all of the "pericarp" (fruit wall) is fleshy at maturity are "simple fleshy fruits". Types of simple, fleshy, fruits (with examples) include: An aggregate fruit, or "etaerio", develops from a single flower with numerous simple pistils. The pome fruits of the family Rosaceae, (including apples, pears, rosehips, and saskatoon berry) are a syncarpous fleshy fruit, a simple fruit, developing from a half-inferior ovary. Schizocarp fruits form from a syncarpous ovary and do not really dehisce, but rather split into segments with one or more seeds; they include a number of different forms from a wide range