Bt brinjal from non-transgenic, and "aad" is used to identify the transformed bacteria used during the development of the construct. "Aad" contains a bacterial promoter and is not expressed in the Bt brinjal. The completed construct was inserted into young cotyledons from the brinjal plants using an "Agrobacterium"-mediated technique. "Agrobacterium" naturally inserts DNA into plants from its Ti plasmid, and scientists use this to insert genes of interest into various plants. The transformed plants were regenerated and analyzed for the presence of the gene through Southern blotting. The plants' progeny were also analyzed to identify lines segregating in a Mendelian fashion. When
Gamco, Inc. v. Providence Fruit & Produce Building, Inc. along the railroad tracks in Providence, R. I., which served as a produce market where the city's wholesale fruit and vegetable trade was concentrated. It was designed to provide selling, storage, and shipping facilities to the fresh fruit and vegetable dealers of Providence who had found themselves forced from the downtown city streets by accumulating traffic congestion. The facilities are particularly advantageous to local wholesalers. The shipping facilities are the best in Providence. The wholesaler tenants of the building were stockholders of PF&PB. All the leases contained a clause under which the tenants agreed not to "transfer or permit to