Bt brinjal from non-transgenic, and "aad" is used to identify the transformed bacteria used during the development of the construct. "Aad" contains a bacterial promoter and is not expressed in the Bt brinjal. The completed construct was inserted into young cotyledons from the brinjal plants using an "Agrobacterium"-mediated technique. "Agrobacterium" naturally inserts DNA into plants from its Ti plasmid, and scientists use this to insert genes of interest into various plants. The transformed plants were regenerated and analyzed for the presence of the gene through Southern blotting. The plants' progeny were also analyzed to identify lines segregating in a Mendelian fashion. When
Indian name use initials besides their name except for when caste names are given more preference by certain families rather than the family name itself. According to the Chicago Manual of Style, Indian names are usually indexed by the family name, with the family name separated from the other names by a comma, but indexing may differ according to the local usage and the preferences of the individual. Indian name Indian names are based on a variety of systems and naming conventions, which vary from region to region. Names are also influenced by religion and caste and may come from epics. India's