Bt brinjal from non-transgenic, and "aad" is used to identify the transformed bacteria used during the development of the construct. "Aad" contains a bacterial promoter and is not expressed in the Bt brinjal. The completed construct was inserted into young cotyledons from the brinjal plants using an "Agrobacterium"-mediated technique. "Agrobacterium" naturally inserts DNA into plants from its Ti plasmid, and scientists use this to insert genes of interest into various plants. The transformed plants were regenerated and analyzed for the presence of the gene through Southern blotting. The plants' progeny were also analyzed to identify lines segregating in a Mendelian fashion. When
Fruit by the Foot and flavors are used to synthesize the various flavors that Fruit by the Foot offers; for example, the strawberry variety contains no actual strawberries. Meanwhile, the British equivalent, Kellogg's Fruit Winders, contains real strawberry purée and uses plant-based colorants. Fruit by the Foot contains no protein, fiber, iron, calcium, or Vitamin A. Since the 1990s, the paper backing has been printed with games, jokes, or trivia facts – though not all flavors have it, such as 'Rippin Berry Berry', and 'Berry Tie-dye'. In early 1999, Nintendo and General Mills worked together on a promotional television advertising campaign costing $5 million.