Bt brinjal from non-transgenic, and "aad" is used to identify the transformed bacteria used during the development of the construct. "Aad" contains a bacterial promoter and is not expressed in the Bt brinjal. The completed construct was inserted into young cotyledons from the brinjal plants using an "Agrobacterium"-mediated technique. "Agrobacterium" naturally inserts DNA into plants from its Ti plasmid, and scientists use this to insert genes of interest into various plants. The transformed plants were regenerated and analyzed for the presence of the gene through Southern blotting. The plants' progeny were also analyzed to identify lines segregating in a Mendelian fashion. When
Marrow (vegetable) Marrow (vegetable) A marrow is a vegetable, the mature fruit of certain "Cucurbita pepo" cultivars. The immature fruit of the same or similar cultivars is called "courgette" (in Britain, Ireland, France, the Netherlands, Singapore, Malaysia and New Zealand) or "zucchini" (in North America, Australia, Italy, Germany and Austria). Like courgettes, marrows are oblong, green squash, but marrows have a firm rind and a neutral flavour ("overgrown when picked and insipid when cooked"), making them useful as edible casings for mincemeat and other stuffings. They can be stored for several weeks after harvest (like pumpkins and other winter squash), to be