Bt brinjal from non-transgenic, and "aad" is used to identify the transformed bacteria used during the development of the construct. "Aad" contains a bacterial promoter and is not expressed in the Bt brinjal. The completed construct was inserted into young cotyledons from the brinjal plants using an "Agrobacterium"-mediated technique. "Agrobacterium" naturally inserts DNA into plants from its Ti plasmid, and scientists use this to insert genes of interest into various plants. The transformed plants were regenerated and analyzed for the presence of the gene through Southern blotting. The plants' progeny were also analyzed to identify lines segregating in a Mendelian fashion. When
Berry (botany) systematic botany. He used eight different terms for fruits, one of which was ' or berry, distinguished from other types of fruit such as ' (drupe) and ' (pome). A ' was defined as "'", meaning "unvalved solid pericarp, containing otherwise naked seeds". The adjective "'" here has the sense of "solid with tissue softer than the outside; stuffed". A berry or ' was distinguished from a drupe and a pome, both of which also had an unvalved solid pericarp; a drupe also contained a nut (') and a pome a capsule ('), rather than the berry's naked seeds. Linnaeus'