Bt brinjal from non-transgenic, and "aad" is used to identify the transformed bacteria used during the development of the construct. "Aad" contains a bacterial promoter and is not expressed in the Bt brinjal. The completed construct was inserted into young cotyledons from the brinjal plants using an "Agrobacterium"-mediated technique. "Agrobacterium" naturally inserts DNA into plants from its Ti plasmid, and scientists use this to insert genes of interest into various plants. The transformed plants were regenerated and analyzed for the presence of the gene through Southern blotting. The plants' progeny were also analyzed to identify lines segregating in a Mendelian fashion. When
Fruit carving give the artwork a different texture or help with its design. Some of the tools include: Before carving a fruit, the necessary materials must be gathered. The usual process is to have the tools handy for when they are needed in one's steps towards creating a carving. There are two types of carvings that can be done. The first is Skin Carving, this is when the outer skin of the fruit (or vegetable) is carved to reveal the fleshy center, where the color is different than the outer skin to create a design using the contrast between the outer skin