Bt brinjal from non-transgenic, and "aad" is used to identify the transformed bacteria used during the development of the construct. "Aad" contains a bacterial promoter and is not expressed in the Bt brinjal. The completed construct was inserted into young cotyledons from the brinjal plants using an "Agrobacterium"-mediated technique. "Agrobacterium" naturally inserts DNA into plants from its Ti plasmid, and scientists use this to insert genes of interest into various plants. The transformed plants were regenerated and analyzed for the presence of the gene through Southern blotting. The plants' progeny were also analyzed to identify lines segregating in a Mendelian fashion. When
The Fruit & Veggie Prescription Program The Fruit & Veggie Prescription Program The Fruit & Vegetable Prescription Program, also known as FVRx, is one of three of Wholesome Wave’s programs. The program aims to provide under-served communities with fresh fruits and vegetables by allowing consumers to exchange healthcare provider-generated “prescriptions” for local fresh fruit and vegetables at participating, local farmers' markets. In 2010, Wholesome Wave implemented a feasibility study where a total of 246 participants were served in five New England cities through work with three healthcare partners and a statewide network of farmers markets during the 2010 farmers' market season. The CAVU foundation, Ceiling and