Bt brinjal from non-transgenic, and "aad" is used to identify the transformed bacteria used during the development of the construct. "Aad" contains a bacterial promoter and is not expressed in the Bt brinjal. The completed construct was inserted into young cotyledons from the brinjal plants using an "Agrobacterium"-mediated technique. "Agrobacterium" naturally inserts DNA into plants from its Ti plasmid, and scientists use this to insert genes of interest into various plants. The transformed plants were regenerated and analyzed for the presence of the gene through Southern blotting. The plants' progeny were also analyzed to identify lines segregating in a Mendelian fashion. When
Wheat berry Wheat berry A wheat berry, or wheatberry, is a whole wheat kernel (except for the hull/husk), composed of the bran, germ, and endosperm. Botanically, it is a type of fruit called a caryopsis. Wheat berries have a tan to reddish-brown color and are available as either a . They are often added to salads or baked into bread to add a crunchy texture. If wheat berries are milled, whole-wheat flour is produced. Wheat berries are the primary ingredient in an Eastern European Christmas porridge called "kutya". In France, cooked durum wheat berries are commonly eaten as a side dish instead