Bt brinjal from non-transgenic, and "aad" is used to identify the transformed bacteria used during the development of the construct. "Aad" contains a bacterial promoter and is not expressed in the Bt brinjal. The completed construct was inserted into young cotyledons from the brinjal plants using an "Agrobacterium"-mediated technique. "Agrobacterium" naturally inserts DNA into plants from its Ti plasmid, and scientists use this to insert genes of interest into various plants. The transformed plants were regenerated and analyzed for the presence of the gene through Southern blotting. The plants' progeny were also analyzed to identify lines segregating in a Mendelian fashion. When
Aggregate fruit make a larger fruit. Aggregate fruits may also be accessory fruits, in which parts of the flower other than the ovary become fleshy and form part of the fruit. The individual parts of an aggregate fruit come in many forms. Common examples are: The components of other aggregate fruit are more difficult to define. For example, sugar apple ("Annona" spp.) fruit are made up of individual berry-like pistils fused with the receptacle. Aggregate fruit An aggregate fruit or etaerio () is a fruit that develops from the merger of several ovaries that were separate in a single flower. In contrast,