Bt brinjal from non-transgenic, and "aad" is used to identify the transformed bacteria used during the development of the construct. "Aad" contains a bacterial promoter and is not expressed in the Bt brinjal. The completed construct was inserted into young cotyledons from the brinjal plants using an "Agrobacterium"-mediated technique. "Agrobacterium" naturally inserts DNA into plants from its Ti plasmid, and scientists use this to insert genes of interest into various plants. The transformed plants were regenerated and analyzed for the presence of the gene through Southern blotting. The plants' progeny were also analyzed to identify lines segregating in a Mendelian fashion. When
Cherie Berry studies there in 1967. She also studied at Gaston College in 1969 and Oakland Community College in 1977. She married Norman H. Berry Jr. and took his last name, becoming Cherie Berry. In 1985 Berry and her husband founded LGM Ltd., a company based in a former billiard hall that manufactured spark-plug wires for cars. After initial financial uncertainty, the venture became very profitable. Norman H. Berry Jr. died in 2006. Berry has received a small following among younger North Carolinians due to her catchy name (when mispronounced as CHAIR-ree Berry, like the fruit) and her picture inside elevators in