Bt brinjal from non-transgenic, and "aad" is used to identify the transformed bacteria used during the development of the construct. "Aad" contains a bacterial promoter and is not expressed in the Bt brinjal. The completed construct was inserted into young cotyledons from the brinjal plants using an "Agrobacterium"-mediated technique. "Agrobacterium" naturally inserts DNA into plants from its Ti plasmid, and scientists use this to insert genes of interest into various plants. The transformed plants were regenerated and analyzed for the presence of the gene through Southern blotting. The plants' progeny were also analyzed to identify lines segregating in a Mendelian fashion. When
Berry Sarbadhikari Berry Sarbadhikari Bijoy Chandra "Berry" Sarbadhikari (died 19 December 1976 at the age of 72 in Bombay) was an Indian cricket commentator, journalist and author. Berry Sarbadhikari was an opening batsman and wicket-keeper for Calcutta University before taking up journalism. He covered 104 Test matches over about fifty years. He was a commentator in All India Radio till 1972. He got the nickname 'Berry' after, on finding a player short in a match, he entered the name "John Berry" (first names of Jack Hobbs) as the last man and eventually played in that place. He was the son of Sushil