Bt brinjal from non-transgenic, and "aad" is used to identify the transformed bacteria used during the development of the construct. "Aad" contains a bacterial promoter and is not expressed in the Bt brinjal. The completed construct was inserted into young cotyledons from the brinjal plants using an "Agrobacterium"-mediated technique. "Agrobacterium" naturally inserts DNA into plants from its Ti plasmid, and scientists use this to insert genes of interest into various plants. The transformed plants were regenerated and analyzed for the presence of the gene through Southern blotting. The plants' progeny were also analyzed to identify lines segregating in a Mendelian fashion. When
Fruit preserves it imparts to the preserve. In America, marmalade is sweet. In general, jam is produced by taking mashed or chopped fruit or vegetable pulp and boiling it with sugar and water. The proportion of sugar and fruit varies according to the type of fruit and its ripeness, but a rough starting point is equal weights of each. When the mixture reaches a temperature of 104 °C (219 °F), the acid and the pectin in the fruit react with the sugar, and the jam will set on cooling. However, most cooks work by trial and error, bringing the mixture to a