Patrick Edward McGovern ca. 7000-6600 B.C. It was a mixed fermented beverage of rice, honey, and grape and/or hawthorn ("Crataegus pinnatifida" and "C. cuneata") fruit. The laboratory and collaborators began studying some of the most ancient chocolate from ancient Mesoamerica (ca. 1400-1100 B.C.) in 2004 Jars from Puerto Escondido in Honduras were in the shape of the cacao ("Theobroma cacao") pod or fruit, as if to advertise the contents as being a chocolate beverage. The fruit's sweet pulp contains about 15% sugar, which naturally ferments to a 5 to 7% alcoholic beverage. This beverage might have led to the domestication of the tree.
Theobroma cacao seed germination of "T. cacao," inhibiting growth of various types of microorganisms (such as different oomycetes, fungi, and bacteria), and preventing rotting by "Phytophthora megakarya". Various plant pests and diseases can cause serious problems for cacao production. The genome of "T. cacao" is diploid, its size is 430 Mbp, and it comprises 10 chromosome pairs (2n=2x=20). In September 2010, a team of scientists announced a draft sequence of the cacao genome (Matina1-6 genotype). In a second, unrelated project, the International Cocoa Genome Sequencing Consortium-ICGS, co-ordinated by CIRAD, first published in December 2010 (online, paper publication in January 2011), the sequence