Bt brinjal from non-transgenic, and "aad" is used to identify the transformed bacteria used during the development of the construct. "Aad" contains a bacterial promoter and is not expressed in the Bt brinjal. The completed construct was inserted into young cotyledons from the brinjal plants using an "Agrobacterium"-mediated technique. "Agrobacterium" naturally inserts DNA into plants from its Ti plasmid, and scientists use this to insert genes of interest into various plants. The transformed plants were regenerated and analyzed for the presence of the gene through Southern blotting. The plants' progeny were also analyzed to identify lines segregating in a Mendelian fashion. When
Berry (botany) ') was originally used for "any small round fruit". Andrea Caesalpinus (1519–1603) classified plants into trees and herbs, further dividing them by properties of their flowers and fruit. He did not make the modern distinction between "fruits" and "seeds", calling hard structures like nuts ' or seeds. A fleshy fruit was called a '. For Caesalpinus, a true ' or berry was a ' derived from a flower with a superior ovary; one derived from a flower with an inferior ovary was called a '. In 1751, Carl Linnaeus wrote "Philosophia Botanica", considered to be the first textbook of descriptive