Bt brinjal from non-transgenic, and "aad" is used to identify the transformed bacteria used during the development of the construct. "Aad" contains a bacterial promoter and is not expressed in the Bt brinjal. The completed construct was inserted into young cotyledons from the brinjal plants using an "Agrobacterium"-mediated technique. "Agrobacterium" naturally inserts DNA into plants from its Ti plasmid, and scientists use this to insert genes of interest into various plants. The transformed plants were regenerated and analyzed for the presence of the gene through Southern blotting. The plants' progeny were also analyzed to identify lines segregating in a Mendelian fashion. When
Vegetable box scheme Vegetable box scheme A vegetable box scheme is an operation that delivers fresh fruit and vegetables, often locally grown and organic, either directly to the customer or to a local collection point. Typically the produce is sold as an ongoing weekly subscription and the offering may vary week to week depending on what is in season. The first organic box scheme was created by Guy Watson in 1987, who delivered fresh produce locally from Riverford farm. These schemes are usually operated by the grower or a small co-operative. There are approximately 600 such schemes operating in the UK and by