Bt brinjal from non-transgenic, and "aad" is used to identify the transformed bacteria used during the development of the construct. "Aad" contains a bacterial promoter and is not expressed in the Bt brinjal. The completed construct was inserted into young cotyledons from the brinjal plants using an "Agrobacterium"-mediated technique. "Agrobacterium" naturally inserts DNA into plants from its Ti plasmid, and scientists use this to insert genes of interest into various plants. The transformed plants were regenerated and analyzed for the presence of the gene through Southern blotting. The plants' progeny were also analyzed to identify lines segregating in a Mendelian fashion. When
Vegetable oils as alternative energy bio-diesel is that it can tolerate low rainfall (as little as 250 ml per year), far below what most food-crops require, thus reducing yet more their potential to compete. Some species of algae contain as much as 50% vegetable oil. Algae have very high growth rates compared to plants normally used to produce vegetable oil. Potentially algae could produce much more oil per area of land than current farming methods. So producing vegetable oil this way should result in less deforestation and less competition for food production land. One expert wrote: "As demonstrated here, microalgal biodiesel is technically feasible. It