Bt brinjal from non-transgenic, and "aad" is used to identify the transformed bacteria used during the development of the construct. "Aad" contains a bacterial promoter and is not expressed in the Bt brinjal. The completed construct was inserted into young cotyledons from the brinjal plants using an "Agrobacterium"-mediated technique. "Agrobacterium" naturally inserts DNA into plants from its Ti plasmid, and scientists use this to insert genes of interest into various plants. The transformed plants were regenerated and analyzed for the presence of the gene through Southern blotting. The plants' progeny were also analyzed to identify lines segregating in a Mendelian fashion. When
West Side Community Garden Mall Gardeners, Riverside park gardeners, the 111th Street garden and other neighborhood Gardens. In the gated vegetable garden area near West 90th Street, are 87 individual vegetable beds,6 school class plots, a berry patch and a Rose garden. There are also community compost bins by the "Compost Gate" where neighbors may deposit their coffee grounds and other vegetable waste. Along the west fence is a community herb bed, and espaliered fruit trees. There are more fruit trees planted along 90th Street and a large Persimmon tree showers down sweet golden fruit each fall. Outside the Vegetable fence is a walkway