Bt brinjal from non-transgenic, and "aad" is used to identify the transformed bacteria used during the development of the construct. "Aad" contains a bacterial promoter and is not expressed in the Bt brinjal. The completed construct was inserted into young cotyledons from the brinjal plants using an "Agrobacterium"-mediated technique. "Agrobacterium" naturally inserts DNA into plants from its Ti plasmid, and scientists use this to insert genes of interest into various plants. The transformed plants were regenerated and analyzed for the presence of the gene through Southern blotting. The plants' progeny were also analyzed to identify lines segregating in a Mendelian fashion. When
Passover Seder the father of the house, but all Seder participants may participate by reciting the Kiddush and drinking at least a majority of the first cup of wine. Technically, according to Jewish law, whenever one partakes of fruits or vegetables dipped in liquid while remaining wet, one must wash one's hands if the fruit or vegetable remains wet. However, at other times of the year, one has either already washed their hands before eating bread, or dry the fruit or vegetable, in which case one need not wash their hands before eating the fruit or vegetable. According to most traditions, no