Bt brinjal from non-transgenic, and "aad" is used to identify the transformed bacteria used during the development of the construct. "Aad" contains a bacterial promoter and is not expressed in the Bt brinjal. The completed construct was inserted into young cotyledons from the brinjal plants using an "Agrobacterium"-mediated technique. "Agrobacterium" naturally inserts DNA into plants from its Ti plasmid, and scientists use this to insert genes of interest into various plants. The transformed plants were regenerated and analyzed for the presence of the gene through Southern blotting. The plants' progeny were also analyzed to identify lines segregating in a Mendelian fashion. When
Vegetable carving Vegetable carving Vegetable carving is the art of carving vegetables to form beautiful objects, such as flowers or birds. The origins of vegetable carving are disputed: some believe it to have begun in Japan in ancient times, others believe it to have begun in Sukothai, Thailand 700 years ago, while still others believe that vegetable carving originated in the time of the Tang dynasty (AD 618-906) and the Song dynasty (AD 960-1279) in China. Japan has also been referred to as the root of the art of fruit and vegetable carving, called Mukimono in Japanese. According to the book "Japanese