Bt brinjal from non-transgenic, and "aad" is used to identify the transformed bacteria used during the development of the construct. "Aad" contains a bacterial promoter and is not expressed in the Bt brinjal. The completed construct was inserted into young cotyledons from the brinjal plants using an "Agrobacterium"-mediated technique. "Agrobacterium" naturally inserts DNA into plants from its Ti plasmid, and scientists use this to insert genes of interest into various plants. The transformed plants were regenerated and analyzed for the presence of the gene through Southern blotting. The plants' progeny were also analyzed to identify lines segregating in a Mendelian fashion. When
Fresh Fruit and Vegetable Program from local growers, and allows schools to allocate some of their Food Distribution Program funds to fresh produce. Schools involved in the DoA's FFVP are allowed to use funds from the DoD's FFVP in order to purchase fresh produce. Fresh Fruit and Vegetable Program The Fresh Fruit and Vegetable Program (FFVP) is a federally assisted program created by the United States Department of Agriculture (USDA) to help subsidize the purchase of fresh fruits and vegetables during non-meal times during school. The FFVP was created to improve children's diets both at school and at home, increase the variety of foods available