Theobroma cacao coming from Chuao were awarded an appellation of origin under the title "Cacao de Chuao" (from Spanish-"cacao of Chuao"). The cacao bean in 80% of chocolate is made using beans of the Forastero group, the main and most ubiquitous variety being the Amenolado variety, while the arriba variety (such as the Nacional variety) are less commonly found in Forastero produce. Forastero trees are significantly hardier and more disease-resistant than Criollo trees, resulting in cheaper cacao beans. Major cocoa bean processors include Hershey's, Nestlé and Mars, all of which purchase cocoa beans via various sources. Chocolate can be made from "T.
Theobroma cacao seed germination of "T. cacao," inhibiting growth of various types of microorganisms (such as different oomycetes, fungi, and bacteria), and preventing rotting by "Phytophthora megakarya". Various plant pests and diseases can cause serious problems for cacao production. The genome of "T. cacao" is diploid, its size is 430 Mbp, and it comprises 10 chromosome pairs (2n=2x=20). In September 2010, a team of scientists announced a draft sequence of the cacao genome (Matina1-6 genotype). In a second, unrelated project, the International Cocoa Genome Sequencing Consortium-ICGS, co-ordinated by CIRAD, first published in December 2010 (online, paper publication in January 2011), the sequence