Bt brinjal from non-transgenic, and "aad" is used to identify the transformed bacteria used during the development of the construct. "Aad" contains a bacterial promoter and is not expressed in the Bt brinjal. The completed construct was inserted into young cotyledons from the brinjal plants using an "Agrobacterium"-mediated technique. "Agrobacterium" naturally inserts DNA into plants from its Ti plasmid, and scientists use this to insert genes of interest into various plants. The transformed plants were regenerated and analyzed for the presence of the gene through Southern blotting. The plants' progeny were also analyzed to identify lines segregating in a Mendelian fashion. When
Ilama (fruit) Ilama (fruit) The ilama or ilama tree ("Annona diversifolia") is a tropical fruit tree found in Central America. The name is derived from the Nahuatl "ilamatzapotl", of which the rough translation is "old woman's "sapote"". The name is also applied to a similar fruit, "soncoya" or "cabeza de negro" ("Annona purpurea"). The ilama fruit is eaten halved, by scooping the flesh out of the rind, and usually chilled when served. It is sometimes served with cream and sugar to intensify the flavor, or with a drop of lime or lemon juice to highlight a tart and bitter note. The ilama