Bt brinjal from non-transgenic, and "aad" is used to identify the transformed bacteria used during the development of the construct. "Aad" contains a bacterial promoter and is not expressed in the Bt brinjal. The completed construct was inserted into young cotyledons from the brinjal plants using an "Agrobacterium"-mediated technique. "Agrobacterium" naturally inserts DNA into plants from its Ti plasmid, and scientists use this to insert genes of interest into various plants. The transformed plants were regenerated and analyzed for the presence of the gene through Southern blotting. The plants' progeny were also analyzed to identify lines segregating in a Mendelian fashion. When
Berry care is often taken so as to not burst the berries. Frozen or dried berries may be preferable for some baked berry products. Fresh berries are also often incorporated into baked berry desserts, sometimes with cream, either as a filling to the dessert or as a topping. Berries are often added to water and/or juiced, as in cranberry juice, which accounts for 95% of cranberry crop usage, blueberry juice, raspberry juice, goji berry juice, acai juice, aronia berry juice, and strawberry juice. Wine is the principal fermented beverage made from berries (grapes). Fruit wines are commonly made out of other