Nucleic acid hybridization Nucleic acid hybridization In molecular biology, hybridization (or hybridisation) is a phenomenon in which single-stranded deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) molecules anneal to complementary DNA or RNA. Though a double-stranded DNA sequence is generally stable under physiological conditions, changing these conditions in the laboratory (generally by raising the surrounding temperature) will cause the molecules to separate into single strands. These strands are complementary to each other but may also be complementary to other sequences present in their surroundings. Lowering the surrounding temperature allows the single-stranded molecules to anneal or “hybridize” to each other. DNA replication and transcription of
Glyoxylic acid Glyoxylic acid Glyoxylic acid or oxoacetic acid is an organic compound. Together with acetic acid, glycolic acid, and oxalic acid, glyoxylic acid is one of the C carboxylic acids. It is a colourless solid that occurs naturally and is useful industrially. Glyoxylic acid is usually described with the chemical formula OCHCOH, i.e. containing an aldehyde functional group (see image in upper right). The aldehyde in fact is not observed in solution or as a solid. As seen for many other aldehydes, it exists most commonly as the hydrate. Thus, the formula for glyoxylic acid is really (HO)CHCOH, described as the