Nucleic acid hybridization Nucleic acid hybridization In molecular biology, hybridization (or hybridisation) is a phenomenon in which single-stranded deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) molecules anneal to complementary DNA or RNA. Though a double-stranded DNA sequence is generally stable under physiological conditions, changing these conditions in the laboratory (generally by raising the surrounding temperature) will cause the molecules to separate into single strands. These strands are complementary to each other but may also be complementary to other sequences present in their surroundings. Lowering the surrounding temperature allows the single-stranded molecules to anneal or “hybridize” to each other. DNA replication and transcription of
Performic acid Performic acid Performic acid (PFA) is an organic compound with the formula CHO. It is an unstable colorless liquid which can be produced by mixing formic acid with hydrogen peroxide. Owing to its oxidizing and disinfecting action, it is used in the chemical, medical and food industries. Performic acid is a colorless liquid soluble in water, alcohols, ether, benzene, chloroform and other organic solvents. Its strong oxidizing properties are used for cleaving disulfide bonds in protein mapping, as well as for epoxidation, hydroxylation and oxidation reactions in organic synthesis. In the medical and food industries, performic acid is commonly used