Nucleic acid hybridization natural positions within a chromosome). In 1969, the two scientists published a paper demonstrating that radioactive copies of a ribosomal DNA sequence could be used to detect complementary DNA sequences in the nucleus of a frog egg. Since those original observations, many refinements have increased the versatility and sensitivity of the procedure to the extent that in situ hybridization is now considered an essential tool in cytogenetics. Nucleic acid hybridization In molecular biology, hybridization (or hybridisation) is a phenomenon in which single-stranded deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) molecules anneal to complementary DNA or RNA. Though a double-stranded DNA
Performic acid Performic acid Performic acid (PFA) is an organic compound with the formula CHO. It is an unstable colorless liquid which can be produced by mixing formic acid with hydrogen peroxide. Owing to its oxidizing and disinfecting action, it is used in the chemical, medical and food industries. Performic acid is a colorless liquid soluble in water, alcohols, ether, benzene, chloroform and other organic solvents. Its strong oxidizing properties are used for cleaving disulfide bonds in protein mapping, as well as for epoxidation, hydroxylation and oxidation reactions in organic synthesis. In the medical and food industries, performic acid is commonly used