Nucleic acid thermodynamics of base incompatibility by quantifying the temperature at which two strands anneal can provide information as to the similarity in base sequence between the two strands being annealed. The complexes may be dissociated by thermal denaturation, also referred to as melting. In the absence of external negative factors, the processes of hybridization and melting may be repeated in succession indefinitely, which lays the ground for polymerase chain reaction. Most commonly, the pairs of nucleic bases A=T and G≡C are formed, of which the latter is more stable. DNA denaturation, also called DNA melting, is the process by which double-stranded deoxyribonucleic
Sulfuric acid "fuming sulfuric acid", "Disulfuric acid" or "oleum" or, less commonly, "Nordhausen acid". Concentrations of oleum are either expressed in terms of % (called % oleum) or as % (the amount made if were added); common concentrations are 40% oleum (109% ) and 65% oleum (114.6% ). Pure is a solid with melting point of 36 °C. Pure sulfuric acid has a vapor pressure of <0.001 mmHg at 25 °C and 1 mmHg at 145.8 °C, and 98% sulfuric acid has a <1 mmHg vapor pressure at 40 °C. Pure sulfuric acid is a viscous clear liquid, like oil, and this