Nucleic acid hybridization natural positions within a chromosome). In 1969, the two scientists published a paper demonstrating that radioactive copies of a ribosomal DNA sequence could be used to detect complementary DNA sequences in the nucleus of a frog egg. Since those original observations, many refinements have increased the versatility and sensitivity of the procedure to the extent that in situ hybridization is now considered an essential tool in cytogenetics. Nucleic acid hybridization In molecular biology, hybridization (or hybridisation) is a phenomenon in which single-stranded deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) molecules anneal to complementary DNA or RNA. Though a double-stranded DNA
Mesoxalic acid and reacts with water to form a product commonly called "mesoxalic acid (mono)hydrate", more properly dihydroxymalonic acid, HO-(C=O)-C(OH)-(C=O)-OH. In product catalogs and other contexts, the terms "mesoxalic acid", "oxomalonic acid", etc. often refer to this "hydrated" compound. In particular, the product traded as "sodium mesoxalate monohydrate" is almost always sodium dihydroxymalonate. Mesoxalic acid can be obtained synthetically by hydrolysis of alloxan with baryta water, by warming caffuric acid with lead acetate solution, or from glycerin diacetate and concentrated nitric acid in the cold. The product can be obtained also by oxidation of tartronic acid It is also prepared by the