Nucleic acid hybridization natural positions within a chromosome). In 1969, the two scientists published a paper demonstrating that radioactive copies of a ribosomal DNA sequence could be used to detect complementary DNA sequences in the nucleus of a frog egg. Since those original observations, many refinements have increased the versatility and sensitivity of the procedure to the extent that in situ hybridization is now considered an essential tool in cytogenetics. Nucleic acid hybridization In molecular biology, hybridization (or hybridisation) is a phenomenon in which single-stranded deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) molecules anneal to complementary DNA or RNA. Though a double-stranded DNA
Carboxylic acid the electron-withdrawing effect of the carbonyl bond, which makes the terminal oxygen-hydrogen bond weaker and thus makes acid dissociation more favorable (lowers pKa). Carboxylic acids can be seen as reduced or alkylated forms of the Lewis acid carbon dioxide; under some circumstances they can be decarboxylated to yield carbon dioxide. Carboxylic acids are commonly identified using their trivial names, and usually have the suffix "-ic acid". IUPAC-recommended names also exist; in this system, carboxylic acids have an "-oic acid" suffix. For example, butyric acid (CHCOH) is butanoic acid by IUPAC guidelines. The "-oic acid" nomenclature detail is based on the