Nucleic acid hybridization Nucleic acid hybridization In molecular biology, hybridization (or hybridisation) is a phenomenon in which single-stranded deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) molecules anneal to complementary DNA or RNA. Though a double-stranded DNA sequence is generally stable under physiological conditions, changing these conditions in the laboratory (generally by raising the surrounding temperature) will cause the molecules to separate into single strands. These strands are complementary to each other but may also be complementary to other sequences present in their surroundings. Lowering the surrounding temperature allows the single-stranded molecules to anneal or “hybridize” to each other. DNA replication and transcription of
Mesoxalic acid and reacts with water to form a product commonly called "mesoxalic acid (mono)hydrate", more properly dihydroxymalonic acid, HO-(C=O)-C(OH)-(C=O)-OH. In product catalogs and other contexts, the terms "mesoxalic acid", "oxomalonic acid", etc. often refer to this "hydrated" compound. In particular, the product traded as "sodium mesoxalate monohydrate" is almost always sodium dihydroxymalonate. Mesoxalic acid can be obtained synthetically by hydrolysis of alloxan with baryta water, by warming caffuric acid with lead acetate solution, or from glycerin diacetate and concentrated nitric acid in the cold. The product can be obtained also by oxidation of tartronic acid It is also prepared by the