Nucleic acid hybridization Nucleic acid hybridization In molecular biology, hybridization (or hybridisation) is a phenomenon in which single-stranded deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) molecules anneal to complementary DNA or RNA. Though a double-stranded DNA sequence is generally stable under physiological conditions, changing these conditions in the laboratory (generally by raising the surrounding temperature) will cause the molecules to separate into single strands. These strands are complementary to each other but may also be complementary to other sequences present in their surroundings. Lowering the surrounding temperature allows the single-stranded molecules to anneal or “hybridize” to each other. DNA replication and transcription of
Prephenic acid higher priority (according to CIP rules) on the two pseudoasymmetric carbons, "i.e." the carboxyl and the hydroxyl groups, in the "cis" configuration, or (1"s", 4"s") according to the new IUPAC stereochemistry rules (2013). The other stereoisomer, "i.e. trans" or, better, (1"r", 4"r"), is called epiprephenic. Prephenic acid Prephenic acid, commonly also known by its anionic form prephenate, is an intermediate in the biosynthesis of the aromatic amino acids phenylalanine and tyrosine, as well as of a large number of secondary metabolites of the shikimate pathway. It is biosynthesized by a [3,3]-sigmatropic Claisen rearrangement of chorismate. Prephenic acid is an example