Nucleic acid hybridization natural positions within a chromosome). In 1969, the two scientists published a paper demonstrating that radioactive copies of a ribosomal DNA sequence could be used to detect complementary DNA sequences in the nucleus of a frog egg. Since those original observations, many refinements have increased the versatility and sensitivity of the procedure to the extent that in situ hybridization is now considered an essential tool in cytogenetics. Nucleic acid hybridization In molecular biology, hybridization (or hybridisation) is a phenomenon in which single-stranded deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) molecules anneal to complementary DNA or RNA. Though a double-stranded DNA
2-Iodobenzoic acid is ejected, yielding a carbocation which is then attacked by highly nucleophilic I anion. The nitrous acid is usually generated "in situ" from sodium nitrite. 2-Iodobenzoic acid 2-Iodobenzoic acid, or "o"-iodobenzoic acid, is an organic compound with the formula ICHCOOH. The synthesis of 2-iodobenzoic acid "via" the diazotization of anthranilic acid is commonly performed in university organic chemistry labs. One of its most common uses is as a precursor for the preparation of IBX and Dess–Martin periodinane, both used as mild oxidants. 2-Iodobenzoic acid can be synthesized "via" a Sandmeyer reaction consisting of the diazotization of anthranilic acid followed by