Nucleic acid thermodynamics of base incompatibility by quantifying the temperature at which two strands anneal can provide information as to the similarity in base sequence between the two strands being annealed. The complexes may be dissociated by thermal denaturation, also referred to as melting. In the absence of external negative factors, the processes of hybridization and melting may be repeated in succession indefinitely, which lays the ground for polymerase chain reaction. Most commonly, the pairs of nucleic bases A=T and G≡C are formed, of which the latter is more stable. DNA denaturation, also called DNA melting, is the process by which double-stranded deoxyribonucleic
Prephenic acid higher priority (according to CIP rules) on the two pseudoasymmetric carbons, "i.e." the carboxyl and the hydroxyl groups, in the "cis" configuration, or (1"s", 4"s") according to the new IUPAC stereochemistry rules (2013). The other stereoisomer, "i.e. trans" or, better, (1"r", 4"r"), is called epiprephenic. Prephenic acid Prephenic acid, commonly also known by its anionic form prephenate, is an intermediate in the biosynthesis of the aromatic amino acids phenylalanine and tyrosine, as well as of a large number of secondary metabolites of the shikimate pathway. It is biosynthesized by a [3,3]-sigmatropic Claisen rearrangement of chorismate. Prephenic acid is an example