Nucleic acid thermodynamics of base incompatibility by quantifying the temperature at which two strands anneal can provide information as to the similarity in base sequence between the two strands being annealed. The complexes may be dissociated by thermal denaturation, also referred to as melting. In the absence of external negative factors, the processes of hybridization and melting may be repeated in succession indefinitely, which lays the ground for polymerase chain reaction. Most commonly, the pairs of nucleic bases A=T and G≡C are formed, of which the latter is more stable. DNA denaturation, also called DNA melting, is the process by which double-stranded deoxyribonucleic
Hexafluorosilicic acid Hexafluorosilicic acid Hexafluorosilicic acid is an inorganic compound with the chemical formula (also written as ). It is a colorless liquid rarely encountered undiluted. Hexafluorosilicic acid has a distinctive sour taste and pungent smell. It is produced naturally on a large scale in volcanoes. It is manufactured as a precursor to aluminum trifluoride and synthetic cryolite. It is commonly used as a source of fluoride for water fluoridation. Salts derived from hexafluorosilicic acid are called hexafluorosilicates. In aqueous solution, the hydronium cation (HO) is traditionally equated with a solvated proton, and as such, the formula for this compound is often