Y'All Want a Single Y'All Want a Single "Y'All Want a Single" is a song written and recorded by American nu metal band Korn for their sixth studio album, "Take a Look in the Mirror". It was released as the album's third single in March 2004, chosen by fans through a poll on the band's official forum. At a running time of three minutes and seventeen seconds, the song contains 89 uses of the word "fuck" with the repeated line "Y'all want a single, say fuck that - fuck that fuck that", although the radio edit replaces the word "fuck" with "suck". "Y'All Want
Single-cell variability impact due to variability in the proteome. Cytometry, surface methods, and microfluidics technologies are the three classes of tools commonly used to profile the proteomes of individual cells. Cytometry allows researchers to isolate cells of interest, and stain 15-30 proteins to measure their location and/or relative abundance. For surface methods, researchers place a single cell on a surface coated with antibodies, which then bind to proteins secreted by the cell and allow them to be measured. Microfluidics methods for proteome analysis immobilize single cells on a microchip and use staining to measure the proteins of interest, or antibodies to bind