A Single Blade of Grass visions were a cheat. The camera would go to a coffee cup and Frank would say, 'The murderer used a coffee cup.' It drove me nuts. What we were trying to do this year was elevate Frank's visions to a dream-like state, so he would have to interpret what he's seeing. There would be more mystical, symbolic imagery that might give him more of a sense of what's going on. I had wanted to strip away the gift for a long time and see if the show really played without it. But we got back into that". "A Single Blade
Single-use bioreactor conventional bioreactors. Instead of a culture vessel made from stainless steel or glass, a single-use bioreactor is equipped with a disposable bag. The disposable bag is usually made of a three-layer plastic foil. One layer is made from Polyethylene terephthalate or LDPE to provide mechanical stability. A second layer made using PVA or PVC acts as a gas barrier. Finally, a contact layer is made from PVA or PP. For medical applications the single-use materials that contact the product must be certified by the European Medicines Agency or similar authorities responsible for other regions. In general there are two different