A Single Blade of Grass visions were a cheat. The camera would go to a coffee cup and Frank would say, 'The murderer used a coffee cup.' It drove me nuts. What we were trying to do this year was elevate Frank's visions to a dream-like state, so he would have to interpret what he's seeing. There would be more mystical, symbolic imagery that might give him more of a sense of what's going on. I had wanted to strip away the gift for a long time and see if the show really played without it. But we got back into that". "A Single Blade
Single-use bioreactor be influenced by a higher energy input (increasing the stirrer speed or the rocking frequency). However, since single-use bioreactors are mainly used for cell culturing, the energy input is limited by the delicate nature of cells. Higher energy input leads to higher shear forces causing the risk of cell damages. Single-use bioreactors are currently available with up to a volume of about 1000 L; that’s why scale up is limited compared to conventional bioreactors. However, a handful of suppliers are now delivering units at the 2,000 liter scale and some suppliers (Sartorius, Xcellerex, Thermo Scientific HyClone and PBS Biotech) are