Sex and the Single Girl Sex and the Single Girl Sex and the Single Girl is a 1962 non-fiction book by American writer Helen Gurley Brown, written as an advice book that encouraged women to become financially independent and experience sexual relationships before or without marriage. The book sold two million copies in three weeks, was sold in 35 countries and has made the "Los Angeles Times", "The New York Times" and "Time" bestseller lists. In 1960 Brown's husband, David Brown, suggested she write a book that discusses "how a single girl goes about having an affair". The book was rejected by several publishing houses
Single-use bioreactor validation procedures can be made easier, and will finally lead to significant cost reductions. The application of single-use bioreactors reduces the risk of cross contamination and enhances the biological and process safety. Single-use applications are especially suitable for any kind of biopharmaceutical product. Single-use bioreactors contain fewer parts compared with conventional bioreactors, so the initial and maintenance costs are reduced. Limiting factor for the use of "some" single-use bioreactors is the achievable oxygen transfer, represented by the specific mass transfer coefficient (k) for the specific phase area (a), resulting in the volumetric oxygen mass transfer coefficient (ka). Theoretically this can