Sex and the Single Girl Sex and the Single Girl Sex and the Single Girl is a 1962 non-fiction book by American writer Helen Gurley Brown, written as an advice book that encouraged women to become financially independent and experience sexual relationships before or without marriage. The book sold two million copies in three weeks, was sold in 35 countries and has made the "Los Angeles Times", "The New York Times" and "Time" bestseller lists. In 1960 Brown's husband, David Brown, suggested she write a book that discusses "how a single girl goes about having an affair". The book was rejected by several publishing houses
Single-use bioreactor conventional bioreactors. Instead of a culture vessel made from stainless steel or glass, a single-use bioreactor is equipped with a disposable bag. The disposable bag is usually made of a three-layer plastic foil. One layer is made from Polyethylene terephthalate or LDPE to provide mechanical stability. A second layer made using PVA or PVC acts as a gas barrier. Finally, a contact layer is made from PVA or PP. For medical applications the single-use materials that contact the product must be certified by the European Medicines Agency or similar authorities responsible for other regions. In general there are two different