Single-handed sailing circumnavigation, as it was contained within the southern hemisphere; however, he made the first single-handed passage of the three great capes, and indeed the first successful single-handed passage of Cape Horn. With only three landfalls, Vito Dumas described the legs of his trip as the longest that had been made by a single-hander, and in the most ferocious oceans on the Earth. Organised single-handed yacht racing was pioneered by Britons "Blondie" Hasler and Francis Chichester, who conceived the idea of a single-handed race across the Atlantic Ocean. This was a revolutionary concept at the time, as the idea was thought
Single-cell variability impact due to variability in the proteome. Cytometry, surface methods, and microfluidics technologies are the three classes of tools commonly used to profile the proteomes of individual cells. Cytometry allows researchers to isolate cells of interest, and stain 15-30 proteins to measure their location and/or relative abundance. For surface methods, researchers place a single cell on a surface coated with antibodies, which then bind to proteins secreted by the cell and allow them to be measured. Microfluidics methods for proteome analysis immobilize single cells on a microchip and use staining to measure the proteins of interest, or antibodies to bind