A Single Girl her a César Award nomination. A Single Girl A Single Girl () is a 1995 French drama film directed by Benoît Jacquot. It follows a day in the life of a young Parisian woman named Valérie (played by Virginie Ledoyen) who begins a new job at a four star hotel the same day she reveals to her boyfriend that she is pregnant. The 90 minute film is shot in real time, with a very mobile camera style, recalling the French New Wave. This was the breakthrough role for the 19-year-old Ledoyen (who later became known in America for the 2000
Single-use bioreactor be influenced by a higher energy input (increasing the stirrer speed or the rocking frequency). However, since single-use bioreactors are mainly used for cell culturing, the energy input is limited by the delicate nature of cells. Higher energy input leads to higher shear forces causing the risk of cell damages. Single-use bioreactors are currently available with up to a volume of about 1000 L; that’s why scale up is limited compared to conventional bioreactors. However, a handful of suppliers are now delivering units at the 2,000 liter scale and some suppliers (Sartorius, Xcellerex, Thermo Scientific HyClone and PBS Biotech) are