Y'All Want a Single industry and major label companies flash across the screen. One caption announces the video itself cost $150,000 to make, a budget smaller than many major label releases at the time. They state opinions based on the idea that the corporate nature of the music industry is questionable. Y'All Want a Single "Y'All Want a Single" is a song written and recorded by American nu metal band Korn for their sixth studio album, "Take a Look in the Mirror". It was released as the album's third single in March 2004, chosen by fans through a poll on the band's official forum.
Single-use bioreactor be influenced by a higher energy input (increasing the stirrer speed or the rocking frequency). However, since single-use bioreactors are mainly used for cell culturing, the energy input is limited by the delicate nature of cells. Higher energy input leads to higher shear forces causing the risk of cell damages. Single-use bioreactors are currently available with up to a volume of about 1000 L; that’s why scale up is limited compared to conventional bioreactors. However, a handful of suppliers are now delivering units at the 2,000 liter scale and some suppliers (Sartorius, Xcellerex, Thermo Scientific HyClone and PBS Biotech) are