A Single Blade of Grass visions were a cheat. The camera would go to a coffee cup and Frank would say, 'The murderer used a coffee cup.' It drove me nuts. What we were trying to do this year was elevate Frank's visions to a dream-like state, so he would have to interpret what he's seeing. There would be more mystical, symbolic imagery that might give him more of a sense of what's going on. I had wanted to strip away the gift for a long time and see if the show really played without it. But we got back into that". "A Single Blade
Single-use bioreactor and other metrics needed to improve yields and product efficacy. New perfusion devices are also becoming popular for certain cell culture applications. Environmental aspects for single-use bioreactors are important to consider due to the amount of disposable material used compared with conventional bioreactors. A complete life cycle assessment comparing single-use bioreactors and conventional bioreactors does not exist, but many ecological reasons are supporting the concept of single-use bioreactors. For a complete life cycle assessment not only the manufacturing, but also the repeated use need to be considered. Even the main part of a single-use bioreactor is not a disposable, but