A Single Girl her a César Award nomination. A Single Girl A Single Girl () is a 1995 French drama film directed by Benoît Jacquot. It follows a day in the life of a young Parisian woman named Valérie (played by Virginie Ledoyen) who begins a new job at a four star hotel the same day she reveals to her boyfriend that she is pregnant. The 90 minute film is shot in real time, with a very mobile camera style, recalling the French New Wave. This was the breakthrough role for the 19-year-old Ledoyen (who later became known in America for the 2000
Single-cell variability impact due to variability in the proteome. Cytometry, surface methods, and microfluidics technologies are the three classes of tools commonly used to profile the proteomes of individual cells. Cytometry allows researchers to isolate cells of interest, and stain 15-30 proteins to measure their location and/or relative abundance. For surface methods, researchers place a single cell on a surface coated with antibodies, which then bind to proteins secreted by the cell and allow them to be measured. Microfluidics methods for proteome analysis immobilize single cells on a microchip and use staining to measure the proteins of interest, or antibodies to bind