Sex and the Single Girl Sex and the Single Girl Sex and the Single Girl is a 1962 non-fiction book by American writer Helen Gurley Brown, written as an advice book that encouraged women to become financially independent and experience sexual relationships before or without marriage. The book sold two million copies in three weeks, was sold in 35 countries and has made the "Los Angeles Times", "The New York Times" and "Time" bestseller lists. In 1960 Brown's husband, David Brown, suggested she write a book that discusses "how a single girl goes about having an affair". The book was rejected by several publishing houses
Single-use bioreactor be influenced by a higher energy input (increasing the stirrer speed or the rocking frequency). However, since single-use bioreactors are mainly used for cell culturing, the energy input is limited by the delicate nature of cells. Higher energy input leads to higher shear forces causing the risk of cell damages. Single-use bioreactors are currently available with up to a volume of about 1000 L; that’s why scale up is limited compared to conventional bioreactors. However, a handful of suppliers are now delivering units at the 2,000 liter scale and some suppliers (Sartorius, Xcellerex, Thermo Scientific HyClone and PBS Biotech) are