A Single Girl her a César Award nomination. A Single Girl A Single Girl () is a 1995 French drama film directed by Benoît Jacquot. It follows a day in the life of a young Parisian woman named Valérie (played by Virginie Ledoyen) who begins a new job at a four star hotel the same day she reveals to her boyfriend that she is pregnant. The 90 minute film is shot in real time, with a very mobile camera style, recalling the French New Wave. This was the breakthrough role for the 19-year-old Ledoyen (who later became known in America for the 2000
Single-use bioreactor conventional bioreactors. Instead of a culture vessel made from stainless steel or glass, a single-use bioreactor is equipped with a disposable bag. The disposable bag is usually made of a three-layer plastic foil. One layer is made from Polyethylene terephthalate or LDPE to provide mechanical stability. A second layer made using PVA or PVC acts as a gas barrier. Finally, a contact layer is made from PVA or PP. For medical applications the single-use materials that contact the product must be certified by the European Medicines Agency or similar authorities responsible for other regions. In general there are two different