Sex and the Single Girl Sex and the Single Girl Sex and the Single Girl is a 1962 non-fiction book by American writer Helen Gurley Brown, written as an advice book that encouraged women to become financially independent and experience sexual relationships before or without marriage. The book sold two million copies in three weeks, was sold in 35 countries and has made the "Los Angeles Times", "The New York Times" and "Time" bestseller lists. In 1960 Brown's husband, David Brown, suggested she write a book that discusses "how a single girl goes about having an affair". The book was rejected by several publishing houses
Single-use bioreactor the pH, and the color, of the dye. The color change can be detected with a laser external to the bag. This and other methods of non-invasive measurement have been developed for single-use bioreactors. Single-use process steps available are: media and buffer preparation, cell harvesting, filtration, purification and virus inactivation. Other manufacturers include Cellexus with their CellMaker with an air lift system for bacterial production,PBS Biotech, Inc. with its novel Air-Wheel mixing mechanism and low shear and scalability from lab to cGMP (Good manufacturing practice) production and is fully integrated, easy to use www.pbsbiotech.com as well as Millipore, which is