Sex and the Single Girl Sex and the Single Girl Sex and the Single Girl is a 1962 non-fiction book by American writer Helen Gurley Brown, written as an advice book that encouraged women to become financially independent and experience sexual relationships before or without marriage. The book sold two million copies in three weeks, was sold in 35 countries and has made the "Los Angeles Times", "The New York Times" and "Time" bestseller lists. In 1960 Brown's husband, David Brown, suggested she write a book that discusses "how a single girl goes about having an affair". The book was rejected by several publishing houses
Single-use bioreactor and other metrics needed to improve yields and product efficacy. New perfusion devices are also becoming popular for certain cell culture applications. Environmental aspects for single-use bioreactors are important to consider due to the amount of disposable material used compared with conventional bioreactors. A complete life cycle assessment comparing single-use bioreactors and conventional bioreactors does not exist, but many ecological reasons are supporting the concept of single-use bioreactors. For a complete life cycle assessment not only the manufacturing, but also the repeated use need to be considered. Even the main part of a single-use bioreactor is not a disposable, but