Y'All Want a Single Y'All Want a Single "Y'All Want a Single" is a song written and recorded by American nu metal band Korn for their sixth studio album, "Take a Look in the Mirror". It was released as the album's third single in March 2004, chosen by fans through a poll on the band's official forum. At a running time of three minutes and seventeen seconds, the song contains 89 uses of the word "fuck" with the repeated line "Y'all want a single, say fuck that - fuck that fuck that", although the radio edit replaces the word "fuck" with "suck". "Y'All Want
Single-use bioreactor be influenced by a higher energy input (increasing the stirrer speed or the rocking frequency). However, since single-use bioreactors are mainly used for cell culturing, the energy input is limited by the delicate nature of cells. Higher energy input leads to higher shear forces causing the risk of cell damages. Single-use bioreactors are currently available with up to a volume of about 1000 L; that’s why scale up is limited compared to conventional bioreactors. However, a handful of suppliers are now delivering units at the 2,000 liter scale and some suppliers (Sartorius, Xcellerex, Thermo Scientific HyClone and PBS Biotech) are