Stewart of Darnley Archibald Douglas, 6th Earl of Angus. Born in England and raised at the court of her uncle Henry VIII, Margaret was Henry's officially recognized heir between the disgrace of Anne Boleyn and the birth of the future Edward VI. In 1565 Henry Stuart, Lord Darnley married his cousin Mary Queen of Scots, like him a descendant of Margaret Tudor and Henry VII. Mary's descent from Margaret Tudor's first marriage to James IV of Scots also placed her in line for the English throne, but many argued that Margaret Douglas's claim was superior as Margaret and her sons Henry Stuart, Lord
Stewart of Darnley east of Barrhead, present day Darnley, within the city of Glasgow. In 1356, Robert Stewart, High Steward of Scotland granted the barony to Sir John Stewart. Like the Royal Stewarts, the Stewarts of Darnley used both the Stuart and the Stewart spelling of their surname. Sir John Stewart of Darnley (d.1429) fought in the Hundred Years' War under his namesake and distant cousin John Stewart, 2nd Earl of Buchan in the army of Charles VII of France. For his services he was rewarded with the lands of Aubigny-sur-Nere and Concressault which were given to the second son in the family