Stewart of Darnley Archibald Douglas, 6th Earl of Angus. Born in England and raised at the court of her uncle Henry VIII, Margaret was Henry's officially recognized heir between the disgrace of Anne Boleyn and the birth of the future Edward VI. In 1565 Henry Stuart, Lord Darnley married his cousin Mary Queen of Scots, like him a descendant of Margaret Tudor and Henry VII. Mary's descent from Margaret Tudor's first marriage to James IV of Scots also placed her in line for the English throne, but many argued that Margaret Douglas's claim was superior as Margaret and her sons Henry Stuart, Lord
Henry Stuart, Lord Darnley of Albany and King James V. In 1668 the vault was opened by mobs and sometime later between 1776-8 the vault was raided and the skull of Lord Darnley stolen. In 1928 a paper was published by Karl Pearson, detailing his vast research into the skull of Lord Darnley. In his paper, Pearson discussed the possibility of Darnley’s skull residing in the Royal College of Surgeons’ museum. In 2016 at the request of the University of Edinburgh research was undertaken to identify whether a skull in the University's collection could be Darnley's stolen remains. Pearson's skull and the University's were