Stewart of Darnley Archibald Douglas, 6th Earl of Angus. Born in England and raised at the court of her uncle Henry VIII, Margaret was Henry's officially recognized heir between the disgrace of Anne Boleyn and the birth of the future Edward VI. In 1565 Henry Stuart, Lord Darnley married his cousin Mary Queen of Scots, like him a descendant of Margaret Tudor and Henry VII. Mary's descent from Margaret Tudor's first marriage to James IV of Scots also placed her in line for the English throne, but many argued that Margaret Douglas's claim was superior as Margaret and her sons Henry Stuart, Lord
Henry Stuart, Lord Darnley the Spanish Ambassador in Paris heard this news, the headlines were that Darnley "had murdered his wife, admitted the exiled heretics, and seized the kingdom." However, on 20 March, Darnley posted a declaration denying all knowledge of or complicity in the Rizzio murder. Mary no longer trusted her husband, and he was disgraced by the kingdom. On 27 March, the Earl of Morton and Lord Ruthven, who were both present at Rizzio's murder and had fled to England, wrote to Cecil claiming that Darnley had initiated the murder plot and recruited them, because of his "heich quarrel" and "deadly hatred"