Stewart of Darnley Archibald Douglas, 6th Earl of Angus. Born in England and raised at the court of her uncle Henry VIII, Margaret was Henry's officially recognized heir between the disgrace of Anne Boleyn and the birth of the future Edward VI. In 1565 Henry Stuart, Lord Darnley married his cousin Mary Queen of Scots, like him a descendant of Margaret Tudor and Henry VII. Mary's descent from Margaret Tudor's first marriage to James IV of Scots also placed her in line for the English throne, but many argued that Margaret Douglas's claim was superior as Margaret and her sons Henry Stuart, Lord
Henry Stuart, Lord Darnley private secretary, David Rizzio, was stabbed 56 times on 9 March 1566 by Darnley and his confederates, Protestant Scottish nobles, in the presence of the seven-months-pregnant queen in her dining room. According to English diplomats Thomas Randolph and the Earl of Bedford, the murder of Rizzio (who was rumoured to be the father of Mary's unborn child) was part of Darnley's bid to force Mary to cede the Crown Matrimonial. Darnley also made a bargain with his allies to advance his claim to the Crown Matrimonial in the Parliament of Scotland in return for restoring their lands and titles. When