Stewart of Darnley Archibald Douglas, 6th Earl of Angus. Born in England and raised at the court of her uncle Henry VIII, Margaret was Henry's officially recognized heir between the disgrace of Anne Boleyn and the birth of the future Edward VI. In 1565 Henry Stuart, Lord Darnley married his cousin Mary Queen of Scots, like him a descendant of Margaret Tudor and Henry VII. Mary's descent from Margaret Tudor's first marriage to James IV of Scots also placed her in line for the English throne, but many argued that Margaret Douglas's claim was superior as Margaret and her sons Henry Stuart, Lord
James VI and I Stuart, Lord Darnley. Both Mary and Darnley were great-grandchildren of Henry VII of England through Margaret Tudor, the older sister of Henry VIII. Mary's rule over Scotland was insecure, and she and her husband, being Roman Catholics, faced a rebellion by Protestant noblemen. During Mary's and Darnley's difficult marriage, Darnley secretly allied himself with the rebels and conspired in the murder of the Queen's private secretary, David Rizzio, just three months before James's birth. James was born on 19 June 1566 at Edinburgh Castle, and as the eldest son and heir apparent of the monarch automatically became Duke of Rothesay