Fetal bovine serum and virus, endotoxin, haemoglobin, IgG and total protein assays. Ethical questions have been raised regarding the blood collection process due to the potential suffering caused to the fetus. Although anoxia or active slaughter could be used to induce unconsciousness or death prior to serum harvesting, exposure of live unborn calves to oxygen can cause them to gain awareness before being killed, resulting in active debate about the ethics of harvesting serum. Fetal bovine serum is commercially available from many manufacturers, and because cells grown in vitro are highly sensitive, customers usually test specific batches to check for suitability for their
Blood, Sweat & Tea Blood, Sweat & Tea Blood, Sweat & Tea is a book by ambulance technician Brian Kellett, writing under the pseudonym Tom Reynolds, about life in the London Ambulance Service. The book is compiled from posts and correspondence from the blog Random Acts of Reality, one of the earliest high-readership blogs in England. The book covers Reynolds's career from 2003–2006, and discusses his career as an emergency medical technician and a rapid responder. Although often extremely critical of NHS policies and management, the book was extremely well received within the European emergency services, and the book (with its associated blog) is