Chlorophyll a chlorophyll biosynthesis. Chlorophyll "a" absorbs light within the violet, blue and red wavelengths while mainly reflecting green. This reflectance gives chlorophyll its green appearance. Accessory photosynthetic pigments broaden the spectrum of light absorbed, increasing the range of wavelengths that can be used in photosynthesis. The addition of chlorophyll "b" next to chlorophyll "a" extends the absorption spectrum. In low light conditions, plants produce a greater ratio of chlorophyll "b" to chlorophyll "a" molecules, increasing photosynthetic yield. Absorption of light by photosynthetic pigments converts photons into chemical energy. Light energy radiating onto the chloroplast strikes the pigments in the thylakoid membrane
Chlorophyll fluorescence Chlorophyll fluorescence Chlorophyll fluorescence is light re-emitted by chlorophyll molecules during return from excited to non-excited states. It is used as an indicator of photosynthetic energy conversion in higher plants, algae and bacteria. Excited chlorophyll dissipates the absorbed light energy by driving photosynthesis (photochemical energy conversion), as heat in non-photochemical quenching or by emission as fluorescence radiation. As these processes are complementary processes, the analysis of chlorophyll fluorescence is an important tool in plant research with a wide spectra of applications. Upon illumination of a dark-adapted leaf, there is a rapid rise in fluorescence from Photosystem II (PSII), followed by