Serum (blood) form of immunotherapy. Blood serum and plasma are some of the largest sources of biomarkers, whether for diagnostics or therapeutics. Its vast dynamic range, further complicated by the presence of lipids, salts, and post-translational modifications, as well multiple mechanisms of degradation, presents challenges in analytical reproducibility, sensitivity, resolution, and potential efficacy. For analysis of biomarkers in blood serum samples, it is possible to do a pre-separation by free-flow electrophoresis that usually consists of a depletion of serum albumin protein. This method enables greater penetration of the proteome via separation of a wide variety of charged or chargeable analytes, ranging from
Blood, toil, tears, and sweat are "It [poetry] is forged slowly and painfully, link by link, with blood and sweat and tears" (Lord Alfred Douglas, 1919), "Blood, sweat, and tear-wrung millions" (Lord Byron, 1823), and "...mollifie/ It with thy teares, or sweat, or blood" (John Donne, 1611). In Latin, Cicero and Livy had used the phrase "sweat and blood". "We are in the preliminary stage of one of the greatest battles in history... That we are in action at many points—in Norway and in Holland—, that we have to be prepared in the Mediterranean. That the air battle is continuous, and that many preparations have