Fetal bovine serum and virus, endotoxin, haemoglobin, IgG and total protein assays. Ethical questions have been raised regarding the blood collection process due to the potential suffering caused to the fetus. Although anoxia or active slaughter could be used to induce unconsciousness or death prior to serum harvesting, exposure of live unborn calves to oxygen can cause them to gain awareness before being killed, resulting in active debate about the ethics of harvesting serum. Fetal bovine serum is commercially available from many manufacturers, and because cells grown in vitro are highly sensitive, customers usually test specific batches to check for suitability for their
Blood, toil, tears, and sweat The motion passed unanimously. This was the first of three speeches which he gave during the period of the Battle of France, which commenced on 10 May. Churchill had used similar phrases earlier, such as "Their sweat, their tears, their blood" in 1931, and "new structures of national life erected upon blood, sweat, and tears". Churchill's sentence, "I have nothing to offer but blood, toil, tears, and sweat," has been called a paraphrase of one uttered on 2 July 1849 by Giuseppe Garibaldi when rallying his revolutionary forces in Rome: "I offer hunger, thirst, forced marches, battle, and death." As