Daniel Gabriel Fahrenheit Hague and was buried there at the Kloosterkerk (Cloister Church). According to Fahrenheit's 1724 article, he determined his scale by reference to three fixed points of temperature. The lowest temperature was achieved by preparing a frigorific mixture of ice, water, and ammonium chloride (a salt), and waiting for it to reach equilibrium. The thermometer then was placed into the mixture and the liquid in the thermometer allowed to descend to its lowest point. The thermometer's reading there was taken as 0 °F. The second reference point was selected as the reading of the thermometer when it was placed in still
IbpB thermometer IbpB thermometer The IbpB thermometer is an RNA thermometer element found in the "ibpAB" operon. The operon contains two heat-shock genes, encoding inclusion body binding proteins A and B (IbpA/B), and is the most drastically upregulated operon under heat-shock in "Escherichia coli". "IbpA" is regulated by a ROSE element found in its 5' UTR, while "IbpB" has its own heat-sensitive cis-regulatory element. The activity of this thermoregulator was confirmed "in vitro" but was not found "in vivo", suggesting more complicated operon regulation exists in bacterial cells. The IbpB protein, whose expression is regulated by the IbpB thermometer, is 48% identical