Fetal bovine serum and virus, endotoxin, haemoglobin, IgG and total protein assays. Ethical questions have been raised regarding the blood collection process due to the potential suffering caused to the fetus. Although anoxia or active slaughter could be used to induce unconsciousness or death prior to serum harvesting, exposure of live unborn calves to oxygen can cause them to gain awareness before being killed, resulting in active debate about the ethics of harvesting serum. Fetal bovine serum is commercially available from many manufacturers, and because cells grown in vitro are highly sensitive, customers usually test specific batches to check for suitability for their
Blood, Sweat & Heels March 23, 2014, with a 90-minute reunion show. Brie Bythewood was dismissed from the series after the first season, and was replaced by two newcomers, Chantelle Fraser and Arzo Anwar. In April 2014, Bravo renewed "Blood, Sweat and Heels" for a second season, which premiered on March 29, 2015. On April 8, 2016, series star Daisy Lewellyn died after battling bile duct cancer. She was 36. She learned of the disease in 2015, after makeup artists noticed an unusual coloring in her eye. In the season two finale, she celebrated completing her radiation treatments. Blood, Sweat & Heels Blood, Sweat