Chlorophyll the last step, the conversion of protochlorophyllide to chlorophyll, is light-dependent and such plants are pale (etiolated) if grown in darkness. Non-vascular plants and green algae have an additional light-independent enzyme and grow green even in darkness. Chlorophyll itself is bound to proteins and can transfer the absorbed energy in the required direction. Protochlorophyllide occurs mostly in the free form and, under light conditions, acts as a photosensitizer, forming highly toxic free radicals. Hence, plants need an efficient mechanism of regulating the amount of chlorophyll precursor. In angiosperms, this is done at the step of aminolevulinic acid (ALA), one of
Light-harvesting complexes of green plants pigment molecules and the energy they absorb is shuttled by resonance energy transfer to a specialized chlorophyll-protein complex known as the reaction center of each photosystem. The reaction center initiates a complex series of chemical reactions that capture energy in the form of chemical bonds. For photosystem II, when either of the two chlorophyll "a" molecules at the reaction center absorb energy, an electron is excited and transferred to an electron acceptor molecule, pheophytin, leaving the chlorophyll "a" in an oxidized state. The oxidised chlorophyll "a" replaces the electrons by photolysis that involves the oxidation of water molecules to oxygen,