Serum (blood) form of immunotherapy. Blood serum and plasma are some of the largest sources of biomarkers, whether for diagnostics or therapeutics. Its vast dynamic range, further complicated by the presence of lipids, salts, and post-translational modifications, as well multiple mechanisms of degradation, presents challenges in analytical reproducibility, sensitivity, resolution, and potential efficacy. For analysis of biomarkers in blood serum samples, it is possible to do a pre-separation by free-flow electrophoresis that usually consists of a depletion of serum albumin protein. This method enables greater penetration of the proteome via separation of a wide variety of charged or chargeable analytes, ranging from
Blood, Sweat and Tears (album) Blood, Sweat and Tears (album) Blood, Sweat and Tears is the fifteenth album by singer Johnny Cash, released in 1963 (see 1963 in music). It is a collection of songs about the American working man. This includes "The Legend of John Henry's Hammer" and "Busted", the latter of which would become a single. Both would also be performed by Cash during his famous 1968 concerts at Folsom Prison and be included in the 1999 extended reissue of the album, "At Folsom Prison". The album was included on the Bear Family Records box set "Come Along and Ride This Train". Additional