Fetal bovine serum specific cell type. When changing from batch to batch it is usual to adapt the cells to the new batch of material, for example, by mixing 50% of the old serum with 50% of the new serum and allowing the cells to acclimatise to the new material. Serum is stored frozen to preserve the stability of components such as growth factors. When serum is thawed, some precipitation may be seen. This is a normal phenomenon and it does not compromise the quality of serum in any way. The precipitate may be removed by transferring the serum to sterile tubes and
Blood, toil, tears, and sweat Blood, toil, tears, and sweat The phrase "blood, toil, tears and sweat" became famous in a speech given by Winston Churchill to the House of Commons of the Parliament of the United Kingdom on 13 May 1940. This was Churchill's first speech on 13 May 1940 to the House of Commons after having been offered the King's commission the previous Friday, to become Prime Minister of the United Kingdom in the first year of World War II. Churchill had replaced Neville Chamberlain on 10 May, and in this speech he asked the House to declare its confidence in his Government.