Mannheim of 1848, the city was a centre for political and revolutionary activity. In 1865, Friedrich Engelhorn founded the "Badische Anilin- und Soda-Fabrik" (Baden Aniline and Soda Factory, BASF) in Mannheim, but the factory was constructed across the Rhine in Ludwigshafen because Mannheim residents feared air pollution from its operations. From this dye factory, BASF has developed into the largest chemical company in the world. After opening a workshop in Mannheim in 1871 and patenting engines from 1878, Karl Benz patented the first motor car in 1886. He was born in Mühlburg (now part of Karlsruhe). The Schütte-Lanz company, founded by
Aniline Blue WS the Mallory's connective tissue stain and Gömöri trichrome stain. It is used in differential staining. Aniline Blue WS Aniline Blue WS, also called aniline blue, China blue, or Soluble blue, is a mixture of methyl blue and water blue. It may also be either one of them. It is a soluble dye used as a biological dye, in fluorescence microscopy, appearing a yellow-green colour after excitation with violet light. It is a mixture of the trisulfonates of triphenyl rosaniline and of diphenyl rosaniline. Aniline blue or its constituents are used to stain collagen, as the fibre stain in Masson's trichrome,