Fetal bovine serum and virus, endotoxin, haemoglobin, IgG and total protein assays. Ethical questions have been raised regarding the blood collection process due to the potential suffering caused to the fetus. Although anoxia or active slaughter could be used to induce unconsciousness or death prior to serum harvesting, exposure of live unborn calves to oxygen can cause them to gain awareness before being killed, resulting in active debate about the ethics of harvesting serum. Fetal bovine serum is commercially available from many manufacturers, and because cells grown in vitro are highly sensitive, customers usually test specific batches to check for suitability for their
Blood, Sweat and Tears (album) Blood, Sweat and Tears (album) Blood, Sweat and Tears is the fifteenth album by singer Johnny Cash, released in 1963 (see 1963 in music). It is a collection of songs about the American working man. This includes "The Legend of John Henry's Hammer" and "Busted", the latter of which would become a single. Both would also be performed by Cash during his famous 1968 concerts at Folsom Prison and be included in the 1999 extended reissue of the album, "At Folsom Prison". The album was included on the Bear Family Records box set "Come Along and Ride This Train". Additional