Fetal bovine serum and virus, endotoxin, haemoglobin, IgG and total protein assays. Ethical questions have been raised regarding the blood collection process due to the potential suffering caused to the fetus. Although anoxia or active slaughter could be used to induce unconsciousness or death prior to serum harvesting, exposure of live unborn calves to oxygen can cause them to gain awareness before being killed, resulting in active debate about the ethics of harvesting serum. Fetal bovine serum is commercially available from many manufacturers, and because cells grown in vitro are highly sensitive, customers usually test specific batches to check for suitability for their
Blood, toil, tears, and sweat Blood, toil, tears, and sweat The phrase "blood, toil, tears and sweat" became famous in a speech given by Winston Churchill to the House of Commons of the Parliament of the United Kingdom on 13 May 1940. This was Churchill's first speech on 13 May 1940 to the House of Commons after having been offered the King's commission the previous Friday, to become Prime Minister of the United Kingdom in the first year of World War II. Churchill had replaced Neville Chamberlain on 10 May, and in this speech he asked the House to declare its confidence in his Government.