Environmental impact of hydraulic fracturing in the United States tuberculosis, kidney disease, autoimmune disorders and airway disease such as asthma and bronchitis. Most of these debilitating and potentially fatal diseases are preventable with occupational control measures regarding respirable crystalline exposure. Hydraulic fracturing uses abundant amounts of sand in the process of fracturing as part of the hydraulic fluid. The fracturing fluid consists of a base fluid, proppant and chemical additives. The majority of proppant used in fracturing are made of silica (sand). Truck loads of sand are delivered to the sites, then loaded to sand movers that are then transferred to a blender that mixes the hydraulic fluid. The
Polyacrylamide gel electrophoresis for protein band detection post gel electrophoresis, uses radioactive isotopes to label proteins, which are then detected by using X-ray film. Western blotting is a process by which proteins separated in the acrylamide gel are electrophoretically transferred to a stable, manipulable membrane such as a nitrocellulose, nylon, or PVDF membrane. It is then possible to apply immunochemical techniques to visualise the transferred proteins, as well as accurately identify relative increases or decreases of the protein of interest. Polyacrylamide gel electrophoresis Polyacrylamide gel electrophoresis (PAGE) is a technique widely used in biochemistry, forensic chemistry, genetics, molecular biology and biotechnology to separate