Guar gum Ganganagar and Hanumangarh in Rajasthan are the major Guar trading markets. The United States has produced 4,600 to 14,000 tonnes of guar over the last 5 years. Texas acreage since 1999 has fluctuated from about 7,000 to 50,000 acres. The world production for guar gum and its derivatives is about 1.0 Million tonnes. Non-food guar gum accounts for about 40% of the total demand. Mainly it is used as a proppant transport / proppant suspending agent in hydraulic fracturing. At its peak the price was $8000 per ton. Chemically, guar gum is an exo-polysaccharide composed of the sugars galactose and
Polyacrylamide gel electrophoresis for protein band detection post gel electrophoresis, uses radioactive isotopes to label proteins, which are then detected by using X-ray film. Western blotting is a process by which proteins separated in the acrylamide gel are electrophoretically transferred to a stable, manipulable membrane such as a nitrocellulose, nylon, or PVDF membrane. It is then possible to apply immunochemical techniques to visualise the transferred proteins, as well as accurately identify relative increases or decreases of the protein of interest. Polyacrylamide gel electrophoresis Polyacrylamide gel electrophoresis (PAGE) is a technique widely used in biochemistry, forensic chemistry, genetics, molecular biology and biotechnology to separate