Pulmonary edema The development of pulmonary edema may be associated with symptoms and signs of "fluid overload"; this is a non-specific term to describe the manifestations of right ventricular failure on the rest of the body and includes peripheral edema (swelling of the legs, in general, of the "pitting" variety, wherein the skin is slow to return to normal when pressed upon), raised jugular venous pressure and hepatomegaly, where the liver is enlarged and may be tender or even pulsatile. Other signs include end-inspiratory crackles (sounds heard at the end of a deep breath) on auscultation and the presence of a third
Tissue culture growth of cells from a tissue from a multicellular organism "in vitro". These cells may be cells isolated from a donor organism, "primary cells", or an immortalised cell line. The cells are bathed in a culture medium, which contains essential nutrients and energy sources necessary for the cells' survival. The term tissue culture is often used interchangeably with cell culture The literal meaning of tissue culture refers to the culturing of tissue pieces, i.e. explant culture. Tissue culture is an important tool for the study of the biology of cells from multicellular organisms. It provides an "in vitro" model of