Isotope fractionation become enriched in the liquid phase while the lighter isotopes (O and H) tend toward the vapor phase. Isotope fractionation Isotope fractionation describes processes that affect the relative abundance of isotopes, often used in isotope geochemistry. Normally, the focus is on stable isotopes of the same element. Isotopic fractionation in the natural environment can be measured by isotope analysis, using isotope-ratio mass spectrometry, to separate different element isotopes on the basis of their mass-to-charge ratio, an important tool to understand natural systems. For example, in biochemistry processes cause a fluctuation in the amount of isotopes of carbon ratios incorporated into
Atomic mass molecular masses by comparing the vapor density of a collection of gases with molecules containing one or more of the chemical element in question. In the 20th century, until the 1960s chemists and physicists used two different atomic-mass scales. The chemists used a "atomic mass unit" (amu) scale such that the natural mixture of oxygen isotopes had an atomic mass 16, while the physicists assigned the same number 16 to only the atomic mass of the most common oxygen isotope (O, containing eight protons and eight neutrons). However, because oxygen-17 and oxygen-18 are also present in natural oxygen this led