Hydraulic fracturing in the United States with hydraulic fracturing activity versus those without, with each well addition increasing rates of accidents by 2% and risk of fatality by .6%. Rig-Up and Rig-Down are terms used to refer to the delivery, construction, dismantling and breakdown of equipment required throughout the hydraulic fracturing process. Incidents and fatalities during rig-up/rig-down operations may result if struck or crushed by the heavy equipment involved. Hazards during Mixing and Injection Workers may be exposed to dangerous chemicals while mixing and injecting fluids used in hydraulic fracturing. Potential chemicals include, hydrochloric and hydrofluoric acid, biocides, methanol, ethylene glycol, guar gum, polysaccharides and polyacrylamides,
Polyacrylamide gel electrophoresis for protein band detection post gel electrophoresis, uses radioactive isotopes to label proteins, which are then detected by using X-ray film. Western blotting is a process by which proteins separated in the acrylamide gel are electrophoretically transferred to a stable, manipulable membrane such as a nitrocellulose, nylon, or PVDF membrane. It is then possible to apply immunochemical techniques to visualise the transferred proteins, as well as accurately identify relative increases or decreases of the protein of interest. Polyacrylamide gel electrophoresis Polyacrylamide gel electrophoresis (PAGE) is a technique widely used in biochemistry, forensic chemistry, genetics, molecular biology and biotechnology to separate