Guar gum Ganganagar and Hanumangarh in Rajasthan are the major Guar trading markets. The United States has produced 4,600 to 14,000 tonnes of guar over the last 5 years. Texas acreage since 1999 has fluctuated from about 7,000 to 50,000 acres. The world production for guar gum and its derivatives is about 1.0 Million tonnes. Non-food guar gum accounts for about 40% of the total demand. Mainly it is used as a proppant transport / proppant suspending agent in hydraulic fracturing. At its peak the price was $8000 per ton. Chemically, guar gum is an exo-polysaccharide composed of the sugars galactose and
Polyacrylamide gel electrophoresis The excess dye incorporated into the gel can be removed by destaining with the same solution without the dye. The proteins are detected as blue bands on a clear background. As SDS is also anionic, it may interfere with staining process. Therefore, large volume of staining solution is recommended, at least ten times the volume of the gel. Ethidium bromide (EtBr) is a popular nucleic acid stain. EtBr allows one to easily visualize DNA or RNA on a gel as EtBr fluoresces an orange color under UV light. Ethidium bromide binds nucleic acid chains through the process of Intercalation. While