Guar the use of guar gum in the hydraulic fracturing (fracking) extraction of oil and shale gas, the demand has increased substantially. Only 10% of the Indian production stays within the country and the remaining 90% is exported for shale gas and oil industries. Consequently, many former cotton or wheat fields are converted into guar fields as production costs are also lower. But the increase of guar gum prices also has other reasons. Guar The Guar or cluster bean, with the botanical name Cyamopsis tetragonoloba, is an annual legume and the source of guar gum. It is also known as Gavar,
Polyacrylamide gel electrophoresis gel. To ensure that the sample sinks to the bottom of the gel, sample buffer is supplemented with additives that increase the density of the sample. These additives should be non-ionic and non-reactive towards proteins to avoid interfering with electrophoresis. Common additives are glycerol and sucrose. Coomassie Brilliant Blue R-250 (CBB)(CHNNaOS; mW: 825.97) is the most popular protein stain. It is an anionic dye, which non-specifically binds to proteins. The structure of CBB is predominantly non-polar, and it is usually used in methanolic solution acidified with acetic acid. Proteins in the gel are fixed by acetic acid and simultaneously stained.