Guar gum Ganganagar and Hanumangarh in Rajasthan are the major Guar trading markets. The United States has produced 4,600 to 14,000 tonnes of guar over the last 5 years. Texas acreage since 1999 has fluctuated from about 7,000 to 50,000 acres. The world production for guar gum and its derivatives is about 1.0 Million tonnes. Non-food guar gum accounts for about 40% of the total demand. Mainly it is used as a proppant transport / proppant suspending agent in hydraulic fracturing. At its peak the price was $8000 per ton. Chemically, guar gum is an exo-polysaccharide composed of the sugars galactose and
Polyacrylamide gel electrophoresis gel. To ensure that the sample sinks to the bottom of the gel, sample buffer is supplemented with additives that increase the density of the sample. These additives should be non-ionic and non-reactive towards proteins to avoid interfering with electrophoresis. Common additives are glycerol and sucrose. Coomassie Brilliant Blue R-250 (CBB)(CHNNaOS; mW: 825.97) is the most popular protein stain. It is an anionic dye, which non-specifically binds to proteins. The structure of CBB is predominantly non-polar, and it is usually used in methanolic solution acidified with acetic acid. Proteins in the gel are fixed by acetic acid and simultaneously stained.