Violin intonation on the harmonics), losing the desired tone, brilliance and intonation. String longevity depends on string quality and playing intensity. A violin is tuned in fifths, in the notes G, D, A, E. The lowest note of a violin, tuned normally, is G, or G below middle C. (On rare occasions, the lowest string may be tuned down by as much as a fourth, to D.) The highest note is less well defined: E, the E two octaves above the open string (which is tuned to E) may be considered a practical limit for orchestral violin parts, but it is
Concert pitch D-flat), and are often tuned to match B brass instruments when the two are required to play together. The Stuttgart Conference of 1834 recommended C264 (A440) as the standard pitch based on Scheibler's studies with his Tonometer. For this reason A440 has been referred to as Stuttgart pitch or Scheibler pitch. In 1939, an international conference recommended that the A above middle C be tuned to 440 Hz, now known as "concert pitch". As a technical standard this was taken up by the International Organization for Standardization in 1955 and reaffirmed by them in 1975 as ISO 16. The difference