Environmental impact of hydraulic fracturing in the United States tuberculosis, kidney disease, autoimmune disorders and airway disease such as asthma and bronchitis. Most of these debilitating and potentially fatal diseases are preventable with occupational control measures regarding respirable crystalline exposure. Hydraulic fracturing uses abundant amounts of sand in the process of fracturing as part of the hydraulic fluid. The fracturing fluid consists of a base fluid, proppant and chemical additives. The majority of proppant used in fracturing are made of silica (sand). Truck loads of sand are delivered to the sites, then loaded to sand movers that are then transferred to a blender that mixes the hydraulic fluid. The
Polyacrylamide gel electrophoresis The excess dye incorporated into the gel can be removed by destaining with the same solution without the dye. The proteins are detected as blue bands on a clear background. As SDS is also anionic, it may interfere with staining process. Therefore, large volume of staining solution is recommended, at least ten times the volume of the gel. Ethidium bromide (EtBr) is a popular nucleic acid stain. EtBr allows one to easily visualize DNA or RNA on a gel as EtBr fluoresces an orange color under UV light. Ethidium bromide binds nucleic acid chains through the process of Intercalation. While