Guar gum is needed for producing sufficient viscosity. Because less is required, costs are reduced. This would help explain its presence in so many things today. In addition to guar gum's effects on viscosity, its high ability to flow, or "deform", gives it very favorable rheological properties. It forms breakable gels when cross-linked with boron. It is used in various multi-phase formulations for hydraulic fracturing, in some as an emulsifier because it helps to prevent oil droplets from coalescing, and in others as a stabilizer to help prevent solid particles from settling and/or separating. Fracking entails the pumping of sand-laden fluids into
Polyacrylamide gel electrophoresis gel. To ensure that the sample sinks to the bottom of the gel, sample buffer is supplemented with additives that increase the density of the sample. These additives should be non-ionic and non-reactive towards proteins to avoid interfering with electrophoresis. Common additives are glycerol and sucrose. Coomassie Brilliant Blue R-250 (CBB)(CHNNaOS; mW: 825.97) is the most popular protein stain. It is an anionic dye, which non-specifically binds to proteins. The structure of CBB is predominantly non-polar, and it is usually used in methanolic solution acidified with acetic acid. Proteins in the gel are fixed by acetic acid and simultaneously stained.