Hydraulic fracturing of chemicals are used per fracturing. A typical fracture treatment uses between 3 and 12 additive chemicals. Although there may be unconventional fracturing fluids, typical chemical additives can include one or more of the following: The most common chemical used for hydraulic fracturing in the United States in 2005–2009 was methanol, while some other most widely used chemicals were isopropyl alcohol, 2-butoxyethanol, and ethylene glycol. Typical fluid types are: For slickwater fluids the use of sweeps is common. Sweeps are temporary reductions in the proppant concentration, which help ensure that the well is not overwhelmed with proppant. As the fracturing
Polyacrylamide gel electrophoresis The excess dye incorporated into the gel can be removed by destaining with the same solution without the dye. The proteins are detected as blue bands on a clear background. As SDS is also anionic, it may interfere with staining process. Therefore, large volume of staining solution is recommended, at least ten times the volume of the gel. Ethidium bromide (EtBr) is a popular nucleic acid stain. EtBr allows one to easily visualize DNA or RNA on a gel as EtBr fluoresces an orange color under UV light. Ethidium bromide binds nucleic acid chains through the process of Intercalation. While