Guar gum needs to be thin enough to make it easier to pump. Then as it flows down the pipe, the fluid needs to gel up to support the proppant and flush it deep into the fractures. After that process, the gel has to break down so we can flow back and recover the fracking fluid, but leave the proppant behind. This requires a chemical process which produces then breaks the gel cross-linking at a predictable rate. Guar+boron+proprietary chemicals can accomplish both of these goals at once. Guar gum retards ice crystal growth nonspecifically by slowing mass transfer across the solid/liquid interface.
Polyacrylamide gel electrophoresis The excess dye incorporated into the gel can be removed by destaining with the same solution without the dye. The proteins are detected as blue bands on a clear background. As SDS is also anionic, it may interfere with staining process. Therefore, large volume of staining solution is recommended, at least ten times the volume of the gel. Ethidium bromide (EtBr) is a popular nucleic acid stain. EtBr allows one to easily visualize DNA or RNA on a gel as EtBr fluoresces an orange color under UV light. Ethidium bromide binds nucleic acid chains through the process of Intercalation. While