Guar the use of guar gum in the hydraulic fracturing (fracking) extraction of oil and shale gas, the demand has increased substantially. Only 10% of the Indian production stays within the country and the remaining 90% is exported for shale gas and oil industries. Consequently, many former cotton or wheat fields are converted into guar fields as production costs are also lower. But the increase of guar gum prices also has other reasons. Guar The Guar or cluster bean, with the botanical name Cyamopsis tetragonoloba, is an annual legume and the source of guar gum. It is also known as Gavar,
Polyacrylamide gel electrophoresis for protein band detection post gel electrophoresis, uses radioactive isotopes to label proteins, which are then detected by using X-ray film. Western blotting is a process by which proteins separated in the acrylamide gel are electrophoretically transferred to a stable, manipulable membrane such as a nitrocellulose, nylon, or PVDF membrane. It is then possible to apply immunochemical techniques to visualise the transferred proteins, as well as accurately identify relative increases or decreases of the protein of interest. Polyacrylamide gel electrophoresis Polyacrylamide gel electrophoresis (PAGE) is a technique widely used in biochemistry, forensic chemistry, genetics, molecular biology and biotechnology to separate