Guar gum an oil or natural gas reservoir at a high pressure and flow rate. This cracks the reservoir rock and then props the cracks open. Water alone is too thin to be effective at carrying proppant sand, so guar gum is one of the ingredients added to thicken the slurry mixture and improve its ability to carry proppant. There are several properties which are important 1. Thixotropic: the fluid should be thixotropic, meaning it should gel within few hours. 2. Gelling and degelling: The desired viscosity changes over the course of a few hours. When the fracking slurry is mixed, it
Polyacrylamide gel electrophoresis gel. To ensure that the sample sinks to the bottom of the gel, sample buffer is supplemented with additives that increase the density of the sample. These additives should be non-ionic and non-reactive towards proteins to avoid interfering with electrophoresis. Common additives are glycerol and sucrose. Coomassie Brilliant Blue R-250 (CBB)(CHNNaOS; mW: 825.97) is the most popular protein stain. It is an anionic dye, which non-specifically binds to proteins. The structure of CBB is predominantly non-polar, and it is usually used in methanolic solution acidified with acetic acid. Proteins in the gel are fixed by acetic acid and simultaneously stained.