Isotope-ratio mass spectrometry isotopic make up of the sample is obtained. For example, carbon isotope ratios are measured relative to the international standard for C. The C standard is produced from a fossil belemnite found in the Peedee Formation, which is a limestone formed in the Cretaceous period in South Carolina, U.S.A. The fossil is referred to as VPDB (Vienna Pee Dee Belemnite) and has C:C ratio of 0.0112372. Oxygen isotope ratios are measured relative the standard, V-SMOW (Vienna Standard Mean Ocean Water). It is critical that the sample be processed before entering the mass spectrometer so that only a single chemical species
Isotope neutron number to atomic number varies the most between isotopes it usually has only a small effect, although it does matter in some circumstances (for hydrogen, the lightest element, the isotope effect is large enough to strongly affect biology). The term "isotopes" (originally also "isotopic elements", now sometimes "isotopic nuclides") is intended to imply comparison (like "synonyms" or "isomers"), for example: the nuclides , , are isotopes (nuclides with the same atomic number but different mass numbers), but , , are isobars (nuclides with the same mass number). However, because "isotope" is the older term, it is better known than