Hydraulic fracturing of chemicals are used per fracturing. A typical fracture treatment uses between 3 and 12 additive chemicals. Although there may be unconventional fracturing fluids, typical chemical additives can include one or more of the following: The most common chemical used for hydraulic fracturing in the United States in 2005–2009 was methanol, while some other most widely used chemicals were isopropyl alcohol, 2-butoxyethanol, and ethylene glycol. Typical fluid types are: For slickwater fluids the use of sweeps is common. Sweeps are temporary reductions in the proppant concentration, which help ensure that the well is not overwhelmed with proppant. As the fracturing
Polyacrylamide gel electrophoresis for protein band detection post gel electrophoresis, uses radioactive isotopes to label proteins, which are then detected by using X-ray film. Western blotting is a process by which proteins separated in the acrylamide gel are electrophoretically transferred to a stable, manipulable membrane such as a nitrocellulose, nylon, or PVDF membrane. It is then possible to apply immunochemical techniques to visualise the transferred proteins, as well as accurately identify relative increases or decreases of the protein of interest. Polyacrylamide gel electrophoresis Polyacrylamide gel electrophoresis (PAGE) is a technique widely used in biochemistry, forensic chemistry, genetics, molecular biology and biotechnology to separate