Pungi fifty years ago. However, prior to this it derived from Indian folk music and was used for religious purposes for snake charming. The pungi is traditionally made from a dried bottle gourd. Often, the neck of the gourd is carved for aesthetic reasons. On the other end, two (sometimes one or three) reed or bamboo pipes are connected. These are known as the "jivala". One of the pipes has 5 - 9 holes and plays the melody; the other pipe is for drone. The jivala is fitted to the gourd with beeswax, which can be adjusted for pitch modification. The
Activity-regulated cytoskeleton-associated protein its activity regulation, localization, and utility as a marker for plastic changes in the brain. Dysfunctions in the production of Arc protein has been implicated as an important factor in understanding of various neurological conditions including amnesia, Alzheimer's disease, Autism spectrum disorders, and Fragile X syndrome. Along with other IEGs such as zif268 and Homer 1a, Arc is also a significant tool for systems neuroscience as illustrated by the development of the "cellular compartment analysis of temporal activity by fluorescence in situ hybridization", or catFISH technique (see fluorescent in situ hybridization). The Arc gene, located on chromosome 15 in the