Pungi fifty years ago. However, prior to this it derived from Indian folk music and was used for religious purposes for snake charming. The pungi is traditionally made from a dried bottle gourd. Often, the neck of the gourd is carved for aesthetic reasons. On the other end, two (sometimes one or three) reed or bamboo pipes are connected. These are known as the "jivala". One of the pipes has 5 - 9 holes and plays the melody; the other pipe is for drone. The jivala is fitted to the gourd with beeswax, which can be adjusted for pitch modification. The
Activity-regulated cytoskeleton-associated protein immediate-early genes. Several transcription factors are known to be involved in regulating the Arc gene (see above), including serum response factor (SRF), CREB, MEF2, and zif268. Changes in Arc mRNA and/or protein are correlated with a number of behavioral changes including cued fear conditioning, contextual fear conditioning, spatial memory, operant conditioning, and inhibitory avoidance. The mRNA is notably upregulated following electrical stimulation in LTP-induction procedures such as high frequency stimulation (HFS), and is massively and globally induced by maximal electroconvulsive shock (MECS). Activity-regulated cytoskeleton-associated protein Activity-regulated cytoskeleton-associated protein is a plasticity protein that in humans is encoded by the ARC