Environmental impact of hydraulic fracturing in the United States tuberculosis, kidney disease, autoimmune disorders and airway disease such as asthma and bronchitis. Most of these debilitating and potentially fatal diseases are preventable with occupational control measures regarding respirable crystalline exposure. Hydraulic fracturing uses abundant amounts of sand in the process of fracturing as part of the hydraulic fluid. The fracturing fluid consists of a base fluid, proppant and chemical additives. The majority of proppant used in fracturing are made of silica (sand). Truck loads of sand are delivered to the sites, then loaded to sand movers that are then transferred to a blender that mixes the hydraulic fluid. The
Polyacrylamide gel electrophoresis gel. To ensure that the sample sinks to the bottom of the gel, sample buffer is supplemented with additives that increase the density of the sample. These additives should be non-ionic and non-reactive towards proteins to avoid interfering with electrophoresis. Common additives are glycerol and sucrose. Coomassie Brilliant Blue R-250 (CBB)(CHNNaOS; mW: 825.97) is the most popular protein stain. It is an anionic dye, which non-specifically binds to proteins. The structure of CBB is predominantly non-polar, and it is usually used in methanolic solution acidified with acetic acid. Proteins in the gel are fixed by acetic acid and simultaneously stained.