Isotope-ratio mass spectrometry isotopic make up of the sample is obtained. For example, carbon isotope ratios are measured relative to the international standard for C. The C standard is produced from a fossil belemnite found in the Peedee Formation, which is a limestone formed in the Cretaceous period in South Carolina, U.S.A. The fossil is referred to as VPDB (Vienna Pee Dee Belemnite) and has C:C ratio of 0.0112372. Oxygen isotope ratios are measured relative the standard, V-SMOW (Vienna Standard Mean Ocean Water). It is critical that the sample be processed before entering the mass spectrometer so that only a single chemical species
Atomic mass molecular masses by comparing the vapor density of a collection of gases with molecules containing one or more of the chemical element in question. In the 20th century, until the 1960s chemists and physicists used two different atomic-mass scales. The chemists used a "atomic mass unit" (amu) scale such that the natural mixture of oxygen isotopes had an atomic mass 16, while the physicists assigned the same number 16 to only the atomic mass of the most common oxygen isotope (O, containing eight protons and eight neutrons). However, because oxygen-17 and oxygen-18 are also present in natural oxygen this led