Columbian exchange As the demand in the New World grew, so did the knowledge on how to cultivate it. The two primary species used were oryza glaberrima and oryza sativa; originating from West Africa and Southeast Asia respectively. Slave holders in the New World relied upon the skills of enslaved Africans to further cultivate both species. . North and South Carolina were key places where rice was grown during the slave trade, and islands of the Caribbean like Puerto Rico and Cuba were equally great centers of production. Enslaved Africans brought their knowledge of water control, milling, winnowing, and other general agrarian
Plant genome assembly products were used to clone remaining gaps, and YACs allowed the characterization of telomere sequences. The resulting sequenced regions were 115.4 Mb of the 125 Mb predicted size of the genome and a total of 25,498 of protein-coding genes. To sequence and assemble the genome of Oryza Sativa (japonica), the same strategy was used. For Oryza Sativa a total of 3,401 mapped clones in a minimum tiling path were selected from the physical map and assembled. One of the most important crops in the world, maize (Zea mays), is the last plant genome project primarily based on Sanger BAC-by-BAC strategy.