Oryza sativa dating from 12,000–11,000 BP indicates that wild rice collection was part of the local means of subsistence. Changes in the morphology of Diaotonghuan phytoliths dating from 10,000–8,000 BP show that rice had by this time been domesticated. Analysis of Chinese rice residues from Pengtoushan, which were carbon 14 dated to 8200–7800 BC, show that rice had been domesticated by this time. In 1998, Crawford and Shen reported the earliest of 14 AMS or radiocarbon dates on rice from at least nine Early to Middle Neolithic sites are no older than 7000 BC, that rice from the Hemudu and Luojiajiao sites
Plant genome assembly products were used to clone remaining gaps, and YACs allowed the characterization of telomere sequences. The resulting sequenced regions were 115.4 Mb of the 125 Mb predicted size of the genome and a total of 25,498 of protein-coding genes. To sequence and assemble the genome of Oryza Sativa (japonica), the same strategy was used. For Oryza Sativa a total of 3,401 mapped clones in a minimum tiling path were selected from the physical map and assembled. One of the most important crops in the world, maize (Zea mays), is the last plant genome project primarily based on Sanger BAC-by-BAC strategy.