Rice ragged stunt virus Rice ragged stunt virus Rice ragged stunt virus (RRSV) is a plant pathogenic virus of the family Reoviridae. RRSV, vectored by the brown planthopper (BPH) (Delphacidae: "Nilaparvata lugens"), causes ragged stunt disease of rice ("Oryza" spp., especially "Oryza sativa"). The virus, first described by Hibino, Ling and Shikata, is also less commonly known as rice infectious gall virus. RRSV has polyhedral particles about 50 nm in diameter possessing spikes (and perhaps a complete outer shell) to a diameter of 65 nm. The particles contain double-stranded RNA in eight segments. RRSV can only infect plants in the family Gramineae, i.e. grasses
Plant genome assembly products were used to clone remaining gaps, and YACs allowed the characterization of telomere sequences. The resulting sequenced regions were 115.4 Mb of the 125 Mb predicted size of the genome and a total of 25,498 of protein-coding genes. To sequence and assemble the genome of Oryza Sativa (japonica), the same strategy was used. For Oryza Sativa a total of 3,401 mapped clones in a minimum tiling path were selected from the physical map and assembled. One of the most important crops in the world, maize (Zea mays), is the last plant genome project primarily based on Sanger BAC-by-BAC strategy.