Glucose are termed glucans. Glucose is produced by plants through the photosynthesis using sunlight, water and carbon dioxide and can be used by all living organisms as an energy and carbon source. However, most glucose does not occur in its free form, but in the form of its polymers, i.e. lactose, sucrose, starch and others which are energy reserve substances, and cellulose and chitin, which are components of the cell wall in plants or fungi and arthropods, respectively. These polymers are degraded to glucose during food intake by animals, fungi and bacteria using enzymes. All animals are also able to produce
Fluorescent glucose biosensor addition to the fluorophore(s), a molecule that confers glucose specificity, in general, a protein. A variety of proteins have been used for this purpose, often with different labs concentrating on one particular protein. The first glucose biosensor reported in the literature was made in 1982 by Schultz’group using a competition assay between glucose and a labelled glucose polymer for the binding site of Concanavalin A entrapped in a hollow dialysis fibre. As a result, Con A was widely used in subsequent sensors in several labs, however Con A suffers from the downside of high toxicity and low reversibility. As a