Hydrogen isotope biogeochemistry assumed that helium-3 was the radioactive component. The work of Luis Walter Alvarez and Robert Cornog first isolated tritium and reversed Rutherford's incorrect notion. Alvarez reasoned that tritium was radioactive, but did not measure the half-life, although calculations at the time suggested over ten years. At the end of World War II, the physical chemist Willard Libby detected the residual radioactivity of a tritium sample with a Geiger counter, providing a more accurate understanding of the half-life, now accepted at 12.3 years. The discovery of hydrogen isotopes also impacted the field of physics in the 1940s, as Nuclear Magnetic Resonance
Hydrogen isotope biogeochemistry this, NMR experiments involved constructing massive projects, locating large magnets, and hand wiring miles of copper coil. Proton NMR remained the most popular technique throughout advancements in following decades, but deuterium and tritium were used in other flavors of NMR spectroscopy. Deuterium has a different magnetic moment and spin than protium, but generally a much smaller signal. Historically, deuterium NMR is a poor alternative to proton NMR, but has been used to study the behavior of lipids on membranes. Recently, a variation of deuterium NMR called H-SNIF has shown potential for understating position specific isotope compositions and comprehending biosynthetic pathways.