Calcofluor-white Calcofluor-white Calcofluor-white is a special fluorescent stain that binds strongly to structures containing cellulose and chitin. In plant cell biology research, it is used for the staining of cell walls of both algae and higher plants. It is also useful in medicine and animal biology for the identification of fungi in the tissue, (it binds to the chitin in the cell walls of the fungi). In research, calcofluor-white is also used to stain bud scars of yeast cells because the bud scars have a higher content of chitin, which stains them more than the rest of the cell membrane. Due
Epiblast-derived stem cell EpiLC was shown to cause a reversion back to an ESC-like state (with levels of Alkaline Phosphatase staining recovered to that observed in ESCs as well as more ESC-like cell morphology), with Klf2 being required for the mechanism to occur. It has been proposed that PRDM14 induces this state by activating Klf2 via active demethylation recruitment of Oct-4; such technique has yet to be demonstrated in actual epiblast-derived EpiSCs. Epiblast-derived stem cell After the blastocyst stage, once an embryo implanted in endometrium (in case of rodent), the inner cell mass (ICM) of a fertilized embryo segregates into two layers: hypoblast