Kinetic Isotope Effects of RuBisCO carbon, oxygen, and/or hydrogen, though currently only a significant isotope effect is seen for carbon isotope substitution. Isotopes are atoms that have the same number of protons but varying numbers of neutrons. "Lighter" isotopes (like the stable Carbon-12 isotope) have a smaller overall mass, and "heavier" isotopes (like the stable Carbon-13 isotope or radioactive Carbon-14 isotope) have a larger overall mass. Stable isotope geochemistry is concerned with how varying chemical and physical processes preferentially enrich or deplete stable isotopes. Enzymes like RuBisCO cause isotopic fractionation because molecules containing lighter isotopes have higher zero-point energies (ZPE), the lowest possible quantum energy
Isotope geochemistry and origins of Earth systems. They are particularly useful to understand mixing processes between different components, because (heavy) radiogenic isotope ratios are not usually fractionated by chemical processes. Radiogenic isotope tracers are most powerful when used together with other tracers: The more tracers used, the more control on mixing processes. An example of this application is to the evolution of the Earth's crust and Earth's mantle through geological time. Lead has four stable isotopes - Pb, Pb, Pb, Pb and one common radioactive isotope Pb with a half-life of ~53,000 years. Lead is created in the Earth via decay of