Isotope geochemistry this reason, enrichments are typically reported in "per mil" (‰, parts per thousand). These enrichments (δ) represent the ratio of heavy isotope to light isotope in the sample over the ratio of a standard. That is, Hydrogen isotope biogeochemistry Carbon has two stable isotopes, C and C, and one radioactive isotope, C. The stable carbon isotope ratio, "δ"C, is measured against Vienna Pee Dee Belemnite (VPDB). The stable carbon isotopes are fractionated primarily by photosynthesis (Faure, 2004). The C/C ratio is also an indicator of paleoclimate: a change in the ratio in the remains of plants indicates a change in
Isotope dilution employed with mass spectrometry in applications where high-accuracy is demanded. For example, all National Metrology Institutes rely significantly on isotope dilution when producing certified reference materials. In addition to high-precision analysis, isotope dilution is applied when low recovery of the analyte is encountered. In addition to the use of stable isotopes, radioactive isotopes can be employed in isotope dilution which is often encountered in biomedical applications, for example, in estimating the volume of blood. Consider a natural analyte rich in isotope A (denoted as A), and the same analyte, enriched in isotope A (denoted as B). Then, the obtained mixture