Pyrolysis oil are affected by temperature, reaction time, algae species, algae concentration, reaction atmosphere, and catalysts, in subcritical water reaction conditions. Bio-oil typically requires significant additional treatment to render it suitable as a refinery feedstock to replace crude oil derived from petroleum, coal-oil, or coal-tar. Tar is a black mixture of hydrocarbons and free carbon obtained from a wide variety of organic materials through destructive distillation. Tar can be produced from coal, wood, petroleum, or peat. Wood-tar creosote is a colourless to yellowish greasy liquid with a smoky odor, produces a sooty flame when burned, and has a burned taste. It is
Coal tar skin can repair itself after short-term exposure to PAHs, but not after long-term exposure. Coal tar was one of the first chemical substances proven to cause cancer from occupational exposure, during research in 1775 on the cause of chimney sweeps' carcinoma. Modern studies have shown that working with coal tar pitch, such as during the paving of roads or when working on roofs, increases the risk of cancer. Coal tar contains many polycyclic aromatic hydrocarbons, and it is believed that their metabolites bind to DNA, damaging it. Long-term skin exposure to these compounds can produce "tar warts", which can progress