Cell encapsulation unit) and N-acetyl-D-glucosamine (acetylated unit). It is derived from the N-deacetylation of chitin and has been used for several applications such as drug delivery, space-filling implants and in wound dressings. However, one drawback of this polymer is its weak mechanical properties and is thus often combined with other polymers such collagen to form a polymer with stronger mechanical properties for cell encapsulation applications. Agarose is a polysaccharide derived from seaweed used for nanoencapsulation of cells and the cell/agarose suspension can be modified to form microbeads by reducing the temperature during preparation. However, one drawback with the microbeads so obtained is
Expression vector as the expression vector in this system. Insect cell lines derived from Lepidopterans (moths and butterflies), such as "Spodoptera frugiperda", are used as host. A cell line derived from the cabbage looper is of particular interest, as it has been developed to grow fast and without the expensive serum normally needed to boost cell growth. The shuttle vector is called bacmid, and gene expression is under the control of a strong promoter pPolh. Baculovirus has also been used with mammalian cell lines in the BacMam system. Baculovirus is normally used for production of glycoproteins, although the glycosylations may be different