Crossover experiment (chemistry) or phosphine. The mechanisms of enzyme-catalyzed reactions can also be studied using crossover experiments. Examples of the application of this technique in biochemistry include the study of reactions catalyzed by nucleoside diphosphohexose-4,6-dehydratases, the aconitase-catalyzed elimination of water from citrate, and various reactions catalyzed by coenzyme B-dependent enzymes, among others. Unlike isotope labeling studies in organic and organometallic chemistry, which typically use deuterium when an isotope of hydrogen is desired, biochemical crossover experiments frequently employ tritium. This is due to the fact that tritium is radioactive and can be tracked using the autoradiographs of gels in gel electrophoresis. Isotope labeling experiments
Tritium radioluminescence for night use and small lights (which need to be more reliable than battery powered lights yet not interfere with night vision or be bright enough to easily give away one's location) used mostly by military personnel fall under the latter application. Tritium lighting is made using glass tubes with a phosphor layer in them and tritium gas inside the tube. Such a tube is known as a "gaseous tritium light source" (GTLS), or "beta light" (since the tritium undergoes beta decay). The tritium in a gaseous tritium light source undergoes beta decay, releasing electrons that cause the phosphor layer