Crossover experiment (chemistry) or phosphine. The mechanisms of enzyme-catalyzed reactions can also be studied using crossover experiments. Examples of the application of this technique in biochemistry include the study of reactions catalyzed by nucleoside diphosphohexose-4,6-dehydratases, the aconitase-catalyzed elimination of water from citrate, and various reactions catalyzed by coenzyme B-dependent enzymes, among others. Unlike isotope labeling studies in organic and organometallic chemistry, which typically use deuterium when an isotope of hydrogen is desired, biochemical crossover experiments frequently employ tritium. This is due to the fact that tritium is radioactive and can be tracked using the autoradiographs of gels in gel electrophoresis. Isotope labeling experiments
Radioactive contamination from the Rocky Flats Plant caused by normal operations rather than accidental releases. From September 1947 to April 1969 there were 5 or more accidental surface water releases of tritium. Tritium, a radioactive element which was found in scrap material from Rocky Flats, was therefore directed in to the Great Western Reservoir. This was uncovered in 1973 and following this, urine samples were taken from people living or working near Broomfield who could have drunk water from the reservoir. The findings of the samples showed that those who were exposed to contaminated water had tritium concentrations near seven times higher than normal (4,300 picocuries per