Kinetic Isotope Effects of RuBisCO carbon, oxygen, and/or hydrogen, though currently only a significant isotope effect is seen for carbon isotope substitution. Isotopes are atoms that have the same number of protons but varying numbers of neutrons. "Lighter" isotopes (like the stable Carbon-12 isotope) have a smaller overall mass, and "heavier" isotopes (like the stable Carbon-13 isotope or radioactive Carbon-14 isotope) have a larger overall mass. Stable isotope geochemistry is concerned with how varying chemical and physical processes preferentially enrich or deplete stable isotopes. Enzymes like RuBisCO cause isotopic fractionation because molecules containing lighter isotopes have higher zero-point energies (ZPE), the lowest possible quantum energy
Atomic vapor laser isotope separation Atomic vapor laser isotope separation Atomic vapor laser isotope separation, or AVLIS, is a method by which specially tuned lasers are used to separate isotopes of uranium using selective ionization of hyperfine transitions. As compared to gas centrifuges the AVLIS process provides high energy efficiency, high separation factors, and a low volume of radioactive waste. A similar technology, using molecules instead of atoms, is molecular laser isotope separation (MLIS). The absorption lines of and U differ slightly due to hyperfine structure; for example, the U absorption peak of 502.74 nanometers shifts to 502.73 nm in U. AVLIS uses tunable dye