Tritium consists of about 4.5 kilograms of plutonium-239. Since tritium undergoes radioactive decay, and is also difficult to confine physically, the much larger secondary charge of heavy hydrogen isotopes needed in a true hydrogen bomb uses solid lithium deuteride as its source of deuterium and tritium, producing the tritium "in situ" during secondary ignition. During the detonation of the primary fission bomb stage in a thermonuclear weapon (Teller-Ullam staging), the sparkplug, a cylinder of U-235/Pu-239 at the center of the fusion stage(s), begins to fission in a chain reaction, from excess neutrons channeled from the primary. The neutrons released from the
Isotope dilution employed with mass spectrometry in applications where high-accuracy is demanded. For example, all National Metrology Institutes rely significantly on isotope dilution when producing certified reference materials. In addition to high-precision analysis, isotope dilution is applied when low recovery of the analyte is encountered. In addition to the use of stable isotopes, radioactive isotopes can be employed in isotope dilution which is often encountered in biomedical applications, for example, in estimating the volume of blood. Consider a natural analyte rich in isotope A (denoted as A), and the same analyte, enriched in isotope A (denoted as B). Then, the obtained mixture