Isotopes of hydrogen used for deuterium and tritium. The IUPAC accepts the D and T symbols, but recommends instead using standard isotopic symbols (H and H) to avoid confusion in the alphabetic sorting of chemical formulas. The ordinary isotope of hydrogen, with no neutrons, is sometimes called "protium". (During the early study of radioactivity, some other heavy radioactive isotopes were given names, H (atomic mass u) is the most common hydrogen isotope with an abundance of more than 99.98%. Because the nucleus of this isotope consists of only a single proton, it is given the formal name "protium". The proton has never been
Isotope dilution employed with mass spectrometry in applications where high-accuracy is demanded. For example, all National Metrology Institutes rely significantly on isotope dilution when producing certified reference materials. In addition to high-precision analysis, isotope dilution is applied when low recovery of the analyte is encountered. In addition to the use of stable isotopes, radioactive isotopes can be employed in isotope dilution which is often encountered in biomedical applications, for example, in estimating the volume of blood. Consider a natural analyte rich in isotope A (denoted as A), and the same analyte, enriched in isotope A (denoted as B). Then, the obtained mixture