Calcofluor-white Calcofluor-white Calcofluor-white is a special fluorescent stain that binds strongly to structures containing cellulose and chitin. In plant cell biology research, it is used for the staining of cell walls of both algae and higher plants. It is also useful in medicine and animal biology for the identification of fungi in the tissue, (it binds to the chitin in the cell walls of the fungi). In research, calcofluor-white is also used to stain bud scars of yeast cells because the bud scars have a higher content of chitin, which stains them more than the rest of the cell membrane. Due
Glucose 6-phosphate precursors of DNA for growth and synthesis, G6P will also be dehydrogenated and enter the pentose phosphate pathway. If the cell needs energy or carbon skeletons for synthesis, then glucose 6-phosphate is targeted for glycolysis. Glucose 6-phosphate is first isomerized to fructose 6-phosphate by phosphoglucose isomerase. This reaction converts glucose 6-phosphate to fructose 6-phosphate in preparation for phosphorylation to fructose-1,6-bisphosphate. The addition of the 2nd phosphoryl group to produce fructose-1,6-bisphosphate is an irreversible step, and so is used to irreversibly target the glucose 6-phosphate breakdown to provide energy for ATP production via glycolysis. If blood glucose levels are high, the