Crossover experiment (chemistry) or phosphine. The mechanisms of enzyme-catalyzed reactions can also be studied using crossover experiments. Examples of the application of this technique in biochemistry include the study of reactions catalyzed by nucleoside diphosphohexose-4,6-dehydratases, the aconitase-catalyzed elimination of water from citrate, and various reactions catalyzed by coenzyme B-dependent enzymes, among others. Unlike isotope labeling studies in organic and organometallic chemistry, which typically use deuterium when an isotope of hydrogen is desired, biochemical crossover experiments frequently employ tritium. This is due to the fact that tritium is radioactive and can be tracked using the autoradiographs of gels in gel electrophoresis. Isotope labeling experiments
Tritium 65 nuclear sites in the US. In one case, leaking water contained of tritium per litre, which is 375 times the EPA limit for drinking water. The US Nuclear Regulatory Commission states that in normal operation in 2003, 56 pressurized water reactors released of tritium (maximum: 2,080 Ci; minimum: 0.1 Ci; average: 725 Ci) and 24 boiling water reactors released (maximum: 174 Ci; minimum: 0 Ci; average: 27.7 Ci), in liquid effluents. According to the U.S. Environmental Protection Agency, self-illuminating exit signs improperly disposed in municipal landfills have been recently found out to contaminate waterways. The legal limits for tritium