Calcofluor-white Calcofluor-white Calcofluor-white is a special fluorescent stain that binds strongly to structures containing cellulose and chitin. In plant cell biology research, it is used for the staining of cell walls of both algae and higher plants. It is also useful in medicine and animal biology for the identification of fungi in the tissue, (it binds to the chitin in the cell walls of the fungi). In research, calcofluor-white is also used to stain bud scars of yeast cells because the bud scars have a higher content of chitin, which stains them more than the rest of the cell membrane. Due
Insect cell culture Insect cell culture The use of insect cell lines as production hosts is an emerging technology for the production of bio pharmaceuticals. There are currently more than 100 insect cell lines available for recombinant protein production with lines derived from "Bombyx mori", "Mamestra brassicae", "Spodoptera frugiperda", "Trichoplusia ni", and "Drosophila melanogaster" being of particular interest. Insects cell lines are commonly used in place of prokaryotic ones because post-translational modifications of proteins are possible in insect cells whereas this mechanism is not present in prokaryotic systems. The Sf9 cell line is one of the most commonly used lines in insect cell