Hydrogen isotope biogeochemistry spectroscopic lines for publishable data, Murphy and Urey paired with Ferdinand Brickwedde and distilled a more concentrated pool of heavy hydrogen, known today as deuterium. This work on hydrogen isotopes won Urey the 1934 Nobel Prize in Chemistry. Also in 1934, scientists Ernest Rutherford, Mark Oliphant, and Paul Harteck, produced the radioactive isotope tritium by hitting deuterium with high energy nuclei. The deuterium used in the experiment was a generous gift of heavy water from the Berkeley physicist Gilbert N Lewis. Bombarding deuterium produced two previously undetected isotopes, helium-3 and hydrogen-3. Rutherford and his colleagues successfully created tritium, but incorrectly
Isotope dilution employed with mass spectrometry in applications where high-accuracy is demanded. For example, all National Metrology Institutes rely significantly on isotope dilution when producing certified reference materials. In addition to high-precision analysis, isotope dilution is applied when low recovery of the analyte is encountered. In addition to the use of stable isotopes, radioactive isotopes can be employed in isotope dilution which is often encountered in biomedical applications, for example, in estimating the volume of blood. Consider a natural analyte rich in isotope A (denoted as A), and the same analyte, enriched in isotope A (denoted as B). Then, the obtained mixture