Kinetic Isotope Effects of RuBisCO carbon, oxygen, and/or hydrogen, though currently only a significant isotope effect is seen for carbon isotope substitution. Isotopes are atoms that have the same number of protons but varying numbers of neutrons. "Lighter" isotopes (like the stable Carbon-12 isotope) have a smaller overall mass, and "heavier" isotopes (like the stable Carbon-13 isotope or radioactive Carbon-14 isotope) have a larger overall mass. Stable isotope geochemistry is concerned with how varying chemical and physical processes preferentially enrich or deplete stable isotopes. Enzymes like RuBisCO cause isotopic fractionation because molecules containing lighter isotopes have higher zero-point energies (ZPE), the lowest possible quantum energy
Hydrogen isotope biogeochemistry constants would allow extrapolation to original isotopic compositions. While this solution holds promise, there is too much disagreement in the literature for robust calibrations. Vapor isotope effects occur for protium, deuterium, and tritium, because each isotope has different thermodynamic properties in the liquid and gaseous phases. For water molecules, the condensed phase is more enriched while the vapor is more depleted. For example, rain condensing from a cloud will be heavier than the vapor starting point. Generally, the large variations in deuterium concentrations of water are from the fractionations between liquid, vapor, and solid reservoirs. In contrast to the fractionation