Properties of water water isotopes. Naturally occurring water is almost completely composed of the neutron-less hydrogen isotope protium. Only 155 ppm include deuterium ( or D), a hydrogen isotope with one neutron, and fewer than 20 parts per quintillion include tritium ( or T), which has two neutrons. Oxygen also has three stable isotopes, with present in 99.76%, in 0.04%, and in 0.2% of water molecules. Deuterium oxide, , is also known as heavy water because of its higher density. It is used in nuclear reactors as a neutron moderator. Tritium is radioactive, decaying with a half-life of 4500 days; exists in nature
Isotope dilution employed with mass spectrometry in applications where high-accuracy is demanded. For example, all National Metrology Institutes rely significantly on isotope dilution when producing certified reference materials. In addition to high-precision analysis, isotope dilution is applied when low recovery of the analyte is encountered. In addition to the use of stable isotopes, radioactive isotopes can be employed in isotope dilution which is often encountered in biomedical applications, for example, in estimating the volume of blood. Consider a natural analyte rich in isotope A (denoted as A), and the same analyte, enriched in isotope A (denoted as B). Then, the obtained mixture