Crossover experiment (chemistry) or phosphine. The mechanisms of enzyme-catalyzed reactions can also be studied using crossover experiments. Examples of the application of this technique in biochemistry include the study of reactions catalyzed by nucleoside diphosphohexose-4,6-dehydratases, the aconitase-catalyzed elimination of water from citrate, and various reactions catalyzed by coenzyme B-dependent enzymes, among others. Unlike isotope labeling studies in organic and organometallic chemistry, which typically use deuterium when an isotope of hydrogen is desired, biochemical crossover experiments frequently employ tritium. This is due to the fact that tritium is radioactive and can be tracked using the autoradiographs of gels in gel electrophoresis. Isotope labeling experiments
Hydrogen isotope biogeochemistry as a relatively weak beta reaction, so the radioactivity cannot permeate skin. Tritium is thus only hazardous if directly ingested or inhaled. Protium is a spin 1/2 subatomic particle and is therefore a fermion. Other fermions include neutrons, electrons, and the radioactive isotope tritium. Fermions are governed by Pauli's exclusion principle, where no two particles can have the same quantum number. However, bosons like deuterium and photons, are not bound by exclusion and multiple particles can occupy the same energy state. This fundamental difference in H and H manifests in many physical properties. Integer spin particles like deuterium follow Bose-Einstein