Calcofluor-white Calcofluor-white Calcofluor-white is a special fluorescent stain that binds strongly to structures containing cellulose and chitin. In plant cell biology research, it is used for the staining of cell walls of both algae and higher plants. It is also useful in medicine and animal biology for the identification of fungi in the tissue, (it binds to the chitin in the cell walls of the fungi). In research, calcofluor-white is also used to stain bud scars of yeast cells because the bud scars have a higher content of chitin, which stains them more than the rest of the cell membrane. Due
Cell encapsulation and agarose have also been employed. Several groups have extensively studied several natural and synthetic polymers with the goal of developing the most suitable biomaterial for cell microencapsulation. Extensive work has been done using alginates which are regarded as the most suitable biomaterials for cell microencapsulation due to their abundance, excellent biocompatibility and biodegradability properties. Alginate is a natural polymer which can be extracted from seaweed and bacteria with numerous compositions based on the isolation source. Alginate is not free from all criticism. Some researchers believe that alginates with high-M content could produce an inflammatory response and an abnormal cell