Kinetic isotope effect both results are large enough to be indicative of weakening of the C-N bond in the transition state that would occur in a concerted process. Since kinetic isotope effects arise from differences in isotopic masses, the largest observable kinetic isotope effects are associated with isotopic substitutions of hydrogen with deuterium (100% increase in mass) or tritium (200% increase in mass). Kinetic isotope effects from isotopic mass ratios can be as large as 36.4 using muons. They have produced the lightest hydrogen atom, H (0.113 amu), in which an electron orbits around a positive muon (μ) "nucleus" that has a mass
Hydrogen isotope biogeochemistry this, NMR experiments involved constructing massive projects, locating large magnets, and hand wiring miles of copper coil. Proton NMR remained the most popular technique throughout advancements in following decades, but deuterium and tritium were used in other flavors of NMR spectroscopy. Deuterium has a different magnetic moment and spin than protium, but generally a much smaller signal. Historically, deuterium NMR is a poor alternative to proton NMR, but has been used to study the behavior of lipids on membranes. Recently, a variation of deuterium NMR called H-SNIF has shown potential for understating position specific isotope compositions and comprehending biosynthetic pathways.