Sudhir Kumar Sopory Polymerase chain reaction-based protocol for manipulating differentially-expressed genes and their promoters. Working on rice (Oryza sativa) and Pennisetum, he evolved new methodologies for producing stress tolerant transgenic plants. His researches on glyoxalase system in plants explained how it affected the stress environment and he propounded a process how transgenic plants capable of growing in conditions of high salinity could be developed by manipulating glyoxalase I and glyoxalase II, two enzymes related to the detoxification of methylglyoxal, the organic compound known to increase the level of stress response of plants. Sopory's researches have been documented in over 250 articles published in
Plant genome assembly products were used to clone remaining gaps, and YACs allowed the characterization of telomere sequences. The resulting sequenced regions were 115.4 Mb of the 125 Mb predicted size of the genome and a total of 25,498 of protein-coding genes. To sequence and assemble the genome of Oryza Sativa (japonica), the same strategy was used. For Oryza Sativa a total of 3,401 mapped clones in a minimum tiling path were selected from the physical map and assembled. One of the most important crops in the world, maize (Zea mays), is the last plant genome project primarily based on Sanger BAC-by-BAC strategy.