Phospholipid-derived fatty acids microbial classes when in fact those PLFAs are present in a broad range of life forms. Phospholipids can occur in many biological classes (such as in plant roots, fungi, as well as in soil bacteria), so care has to be taken in over-assigning PLFA biomarkers to the wrong class. Even though phospholipids occur in many different life forms, the fatty acid side chains between differing life forms can be quite unique. Polyunsaturated fatty acids ("e.g." 18:3 ω3c) are found in plants, algae and cyanobacteria, but are often not present in bacteria. Monounsaturated fatty acids (particularly at the omega-7 position), odd-chain
Green algae were acquired from ingested green algae, and in the latter retain a nucleomorph (vestigial nucleus). Green algae are also found symbiotically in the ciliate "Paramecium", and in "Hydra viridissima" and in flatworms. Some species of green algae, particularly of genera "Trebouxia" of the class "Trebouxiophyceae" and "Trentepohlia" (class Ulvophyceae), can be found in symbiotic associations with fungi to form lichens. In general the fungal species that partner in lichens cannot live on their own, while the algal species is often found living in nature without the fungus. "Trentepohlia" is a filamentous green alga that can live independently on humid soil,