Phospholipid-derived fatty acids microbial classes when in fact those PLFAs are present in a broad range of life forms. Phospholipids can occur in many biological classes (such as in plant roots, fungi, as well as in soil bacteria), so care has to be taken in over-assigning PLFA biomarkers to the wrong class. Even though phospholipids occur in many different life forms, the fatty acid side chains between differing life forms can be quite unique. Polyunsaturated fatty acids ("e.g." 18:3 ω3c) are found in plants, algae and cyanobacteria, but are often not present in bacteria. Monounsaturated fatty acids (particularly at the omega-7 position), odd-chain
Cavalier-Smith's system of classification distinguishes chromists from plants. Cavalier-Smith's eighth kingdom, Archezoa is now defunct. He now assigns former members of the kingdom Archezoa to the phylum Amoebozoa. Cavalier-Smith referred to what remained of the protist kingdom, after he removed the kingdoms Archezoa and Chromista, as the "kingdom Protozoa". In 1993, this kingdom contained 18 phyla as summarized in the following table: The phylum Opalozoa was established by Cavalier-Smith in 1991. By 1998, Cavalier-Smith had reduced the total number of kingdoms from eight to six: Animalia, Protozoa, Fungi, Plantae (including red and green algae), Chromista and Bacteria. Five of Cavalier-Smith's kingdoms are classified as