Nitric acid concentrated acid and nitric oxide for dilute acid. or Concentrated nitric acid oxidizes I, P, and S into HIO, HPO, and HSO, respectively. Nitric acid reacts with proteins to form yellow nitrated products. This reaction is known as the xanthoproteic reaction. This test is carried out by adding concentrated nitric acid to the substance being tested, and then heating the mixture. If proteins that contain amino acids with aromatic rings are present, the mixture turns yellow. Upon adding a base such as ammonia, the color turns orange. These color changes are caused by nitrated aromatic rings in the protein. Xanthoproteic
Acid test (gold) or definitive tests to other materials, became popular during and after the California Gold Rush, but was current before then, as shown by this quote from the Wisconsin paper "The Columbia Reporter", November 1845: "Twenty-four years of service demonstrates his ability "to stand the acid test", as Gibson’s Soap Polish has done for over thirty years." Other examples of the figurative use of the phrase are the web sites Acid1, Acid2 and Acid3, which are designed to test web browsers for compliance with current web standards. Another example is the quick ratio method, nicknamed "acid test", used by financial analysts