Nitric acid concentrated acid and nitric oxide for dilute acid. or Concentrated nitric acid oxidizes I, P, and S into HIO, HPO, and HSO, respectively. Nitric acid reacts with proteins to form yellow nitrated products. This reaction is known as the xanthoproteic reaction. This test is carried out by adding concentrated nitric acid to the substance being tested, and then heating the mixture. If proteins that contain amino acids with aromatic rings are present, the mixture turns yellow. Upon adding a base such as ammonia, the color turns orange. These color changes are caused by nitrated aromatic rings in the protein. Xanthoproteic
Acid–base titration as the equiligraph, have long been used to account for the interaction of coupled equilibria. These graphical solution methods are simple to implement, however they are infrequently used. Simple analytical formulas for the titration of N-protic acids. Valid for any number of N: 1-monoprotic, 2-diprotic until N=6 (e.g. EDTA). Acid–base titration An acid–base titration is a method of quantitative analysis for determining the concentration of an acid or base by exactly neutralizing it with a standard solution of base or acid having known concentration. A pH indicator is used to monitor the progress of the acid–base reaction. If the acid