Acid test (gold) mark is tested by applying aqua fortis (nitric acid), which dissolves the mark of any item that is not gold. If the mark remains, it is tested by applying aqua regia (nitric acid and hydrochloric acid). If the mark is removed, then this test dissolves the gold, proving the item to be genuine gold. More accurate testing of the item for its fineness or purity can be done through the use of differing strengths of aqua regia and comparative testing of gold items of known fineness. The figurative meaning of the expression, where it is applied to tests of character,
Acetoacetic acid widely used in the production of arylide yellows and diarylide dyes. Although the esters can be used in this reaction, diketene also reacts with alcohols and amines to the corresponding acetoacetic acid derivatives in a process called acetoacetylation. An example is the reaction with 2-aminoindane: Acetoacetic acid is measured in the urine of people with diabetes to test for ketoacidosis and for monitoring people on a ketogenic or low-carbohydrate diet, This is done using dipsticks coated in nitroprusside or similar reagents. Nitroprusside changes from pink to purple in the presence of acetoacetate, the conjugate base of acetoacetic acid, and the