Nitric acid concentrated acid and nitric oxide for dilute acid. or Concentrated nitric acid oxidizes I, P, and S into HIO, HPO, and HSO, respectively. Nitric acid reacts with proteins to form yellow nitrated products. This reaction is known as the xanthoproteic reaction. This test is carried out by adding concentrated nitric acid to the substance being tested, and then heating the mixture. If proteins that contain amino acids with aromatic rings are present, the mixture turns yellow. Upon adding a base such as ammonia, the color turns orange. These color changes are caused by nitrated aromatic rings in the protein. Xanthoproteic
Acid–base reaction from an acid produces its "conjugate base", which is the acid with a hydrogen ion removed. The reception of a proton by a base produces its "conjugate acid", which is the base with a hydrogen ion added. Unlike the previous definitions, the Brønsted–Lowry definition does not refer to the formation of salt and solvent, but instead to the formation of "conjugate acids" and "conjugate bases", produced by the transfer of a proton from the acid to the base. In this approach, acids and bases are fundamentally different in behavior from salts, which are seen as electrolytes, subject to the theories