Cloud Nine (Kottonmouth Kings album) by band member Pakelika. It is meant to show that it is their ninth official album and also referred to the so-called "greatness of being high with the heavens". (From informal English: "to be on cloud nine" - to be extremely happy) As of the week of September 15, 2007 the album debuted at number 44 on the U.S. "Billboard" 200 chart, selling about 14,000 copies in its first week, #10 on the "Billboard"s Top Rap Albums, and #3 on the Top Independent Albums chart. The albums also made it to #45 on the Billboard Comprehensive Albums, #44 on the
Heterologous expression expression, on the other hand, refers to the overexpression of a gene in a system from where it originates. Genes are subjected to heterologous expression often to study specific protein interactions. "E. coli", yeast ("S. cerevisiae, P. pastoris"), immortalized mammalian cells, and amphibian oocytes (i.e. unfertilized eggs) are commonly for studies that require heterologous expression. Heterologous expression Heterologous expression refers to the expression of a gene or part of a gene in a host organism, which does not naturally have this gene or gene fragment. Insertion of the gene in the heterologous host is performed by recombinant DNA technology. After