Nitric acid concentrated acid and nitric oxide for dilute acid. or Concentrated nitric acid oxidizes I, P, and S into HIO, HPO, and HSO, respectively. Nitric acid reacts with proteins to form yellow nitrated products. This reaction is known as the xanthoproteic reaction. This test is carried out by adding concentrated nitric acid to the substance being tested, and then heating the mixture. If proteins that contain amino acids with aromatic rings are present, the mixture turns yellow. Upon adding a base such as ammonia, the color turns orange. These color changes are caused by nitrated aromatic rings in the protein. Xanthoproteic
Phosphorous acid concentrating the solution and precipitations with alcohols, the pure acid can be separated from the salt. Phosphorous acid is a strong acid with a p"K" in the range 1.26–1.3. It is a diprotic acid, the hydrogenphosphite ion, HP(O)(OH) is a moderately strong acid: The conjugate base HP(O)(OH) is called hydrogen phosphite, and the second conjugate base, , is the phosphite ion. (Note that the IUPAC recommendations are hydrogen phosphonate and phosphonate respectively). The hydrogen atom bonded directly to the phosphorus atom is not readily ionizable. Chemistry examinations often test students' appreciation of the fact that "not" all three hydrogen atoms