Hinny is easier to obtain when the lower chromosome count, the donkey, is in the male. Therefore breeding for hinnies is more hit-and-miss than breeding for mules." The male hinny or mule can and will mate, but the emission is not fertile. Male hinnies and mules are usually castrated to help control their behavior by eliminating their interest in females. Female hinnies and mules are not customarily spayed, and may or may not go through estrus. Female mules have been known, on rare occasions, to produce offspring when mated to a horse or donkey, although this is extremely uncommon. Since 1527,
Domestication of the horse less than expected based on the overall genetic variation in the remaining genetic material. This indicates that a relatively few stallions were domesticated and that it is unlikely that many male offspring originating from unions between wild stallions and domestic mares were included in early domesticated breeding stock. Genes located in the mitochondrial DNA are passed on along the maternal line from the mother to her offspring. Multiple analyses of the mitochondrial DNA obtained from modern horses as well as from horse bones and teeth from archaeological and palaeological finds consistently shows an increased genetic diversity in the mitochondrial DNA