Minor third augmented thirds span the same number of staff positions, but consist of a different number of semitones (two and five). The minor third is a skip melodically. Notable examples of ascending minor thirds include the opening two notes of "Greensleeves" and of "Light My Fire". The minor third may be derived from the harmonic series as the interval between the fifth and sixth harmonics, or from the 19th harmonic. The minor third is commonly used to express sadness in music, and research shows that this mirrors its use in speech, as a tone similar to a minor third is produced
Major thirds tuning improvisation. In contrast, chords cannot be shifted around the fretboard in standard tuning, which requires four chord-shapes for the major chords: There are separate fingerings for chords having root notes on one of the four strings three–six. The repetition of the major-thirds tuning enables notes and chords to be raised one octave by being "vertically" shifted by three strings. Notes and chords may be shifted "diagonally" in major-thirds tuning, by combining a vertical shift of one string with a horizontal shift of four frets: "Like all regular tunings, chords in the major third tuning can be moved across the fretboard