Nitric acid concentrated acid and nitric oxide for dilute acid. or Concentrated nitric acid oxidizes I, P, and S into HIO, HPO, and HSO, respectively. Nitric acid reacts with proteins to form yellow nitrated products. This reaction is known as the xanthoproteic reaction. This test is carried out by adding concentrated nitric acid to the substance being tested, and then heating the mixture. If proteins that contain amino acids with aromatic rings are present, the mixture turns yellow. Upon adding a base such as ammonia, the color turns orange. These color changes are caused by nitrated aromatic rings in the protein. Xanthoproteic
Acid-base extraction pH range. The order of the step is not important and the process can be repeated to increase the separation. However, it is often convenient to have the compound dissolved in the organic phase after the last step, so that the evaporation of the solvent yields the product. The procedure works only for acids and bases with a large difference in solubility between their charged and their uncharged form. The procedure does not work for: Alternatives to acid-base extraction including: Acid-base extraction Acid-base extraction is a procedure using sequential liquid–liquid extractions to purify acids and bases from mixtures based on