Kyoto Protocol European Union-15, made up of 15 states at the time of the Kyoto negotiations) commit themselves to binding targets for GHG emissions. The targets apply to the four greenhouse gases carbon dioxide (), methane (), nitrous oxide (), sulphur hexafluoride (), and two groups of gases, hydrofluorocarbons (HFCs) and perfluorocarbons (PFCs). The six GHG are translated into CO equivalents in determining reductions in emissions. These reduction targets are in addition to the industrial gases, chlorofluorocarbons, or CFCs, which are dealt with under the 1987 Montreal Protocol on Substances that Deplete the Ozone Layer. Under the Protocol, only the Annex I
Nitrous-oxide reductase competent unit. The Cu center can undergo a one-electron redox change and hence has a function similar to that in the well-known aa-type cytochrome "c" oxidases () where it serves to receive an electron from soluble cytochromes "c". Acetylene is the most specific inhibitor of nitrous-oxide reductase. Other inhibitors include azide anion, thiocyanate, carbon monoxide, iodide, and cyanide. Nitrous-oxide reductase In enzymology, a nitrous oxide reductase also known as nitrogen:acceptor oxidoreductase (NO-forming) is an enzyme that catalyzes the final step in bacterial denitrification, the reduction of nitrous oxide to dinitrogen. It plays a critical role in preventing release of a