Nitric acid concentrated acid and nitric oxide for dilute acid. or Concentrated nitric acid oxidizes I, P, and S into HIO, HPO, and HSO, respectively. Nitric acid reacts with proteins to form yellow nitrated products. This reaction is known as the xanthoproteic reaction. This test is carried out by adding concentrated nitric acid to the substance being tested, and then heating the mixture. If proteins that contain amino acids with aromatic rings are present, the mixture turns yellow. Upon adding a base such as ammonia, the color turns orange. These color changes are caused by nitrated aromatic rings in the protein. Xanthoproteic
Simultaneously extracted metals and Acid-volatile sulfide be determined. In initial versions of the SEM-AVS approach, six metals were measured: nickel, zinc, cadmium, copper, lead, and mercury. More current methods call for the measurement of silver in addition to these metals. In applying the SEM-AVS approach, two concentrations are determined: the total concentrations of all toxic heavy metals of interest, represented as [SEM], and the acid-volatile sulfide concentration, represented as [AVS]. From these concentrations, the [SEM]/[AVS] ratio can be obtained, summarizing the results in a single value. Based on the extremely low K values for heavy metal sulfides, if [SEM]/[AVS] is less than 1 ([SEM < [AVS]),