International Day for the Preservation of the Ozone Layer of the hole in the ozone layer was observed 30 years after the protocol was signed. Due to the nature of the gases responsible for ozone depletion their chemical effects are expected to continue for between 50 to 100 years. International Day for the Preservation of the Ozone Layer September 16 was designated by the United Nations General Assembly as the International Day for the Preservation of the Ozone Layer. This designation had been made on December 19, 2000, in commemoration of the date, in 1987, on which nations signed the Montreal Protocol on Substances that Deplete the Ozone Layer.
Wiley protocol at separate times throughout the period. The protocol is promoted as differing from conventional hormone replacement therapy in several ways: The protocol also includes a follow-up program to monitor the serum of the bioidentical hormones. Wiley has stated that in her opinion, the most important aspect of the protocol is the specific schedule and levels of the hormone doses rather than their bioidentical nature. A patent was granted for the protocol in 2011 for the specific blend of hormones and dosing method. Concerns have been raised that serum levels may not be an acceptable marker for transdermally administered hormones, that