Acid test (gold) mark is tested by applying aqua fortis (nitric acid), which dissolves the mark of any item that is not gold. If the mark remains, it is tested by applying aqua regia (nitric acid and hydrochloric acid). If the mark is removed, then this test dissolves the gold, proving the item to be genuine gold. More accurate testing of the item for its fineness or purity can be done through the use of differing strengths of aqua regia and comparative testing of gold items of known fineness. The figurative meaning of the expression, where it is applied to tests of character,
Methylmalonic acid Methylmalonic acid Methylmalonic acid (MMA) (conjugate base methylmalonate) is a dicarboxylic acid that is a "C"-methylated derivative of malonate. The coenzyme A linked form of methylmalonic acid, methylmalonyl-CoA, is converted into succinyl-CoA by methylmalonyl-CoA mutase, in a reaction that requires vitamin B as a cofactor. In this way, it enters the Krebs cycle, and is thus part of one of the anaplerotic reactions. Increased methylmalonic acid levels may indicate a vitamin B deficiency. However, it is sensitive (those with the disease almost always test positive) but not specific (those that do not have the disease do not always test negative).