Acid test (gold) mark is tested by applying aqua fortis (nitric acid), which dissolves the mark of any item that is not gold. If the mark remains, it is tested by applying aqua regia (nitric acid and hydrochloric acid). If the mark is removed, then this test dissolves the gold, proving the item to be genuine gold. More accurate testing of the item for its fineness or purity can be done through the use of differing strengths of aqua regia and comparative testing of gold items of known fineness. The figurative meaning of the expression, where it is applied to tests of character,
Chromotropic acid uses chromotropic acid. Chromotropic acid Chromotropic acid has the formula is (HO)CH(SOH). It can be used for as a reagent in the quantitative determination of the systemic herbicide, 2,4-dichlorophenoxyacetic acid. Chromotropic acid is also used for testing of presence of formaldehyde. The usefulness of this reagent in quantitative determination is the formation of a red colouration (peaking at 580 nm wavelength) when chromotropic acid in 50% sulfuric acid reacts with formaldehyde. The colouration is specific to this aldehyde and is not produced from other organic species such as ketones and carboxylic acids. The NIOSH Formaldehyde method #3500 is the reference