Acid growth protons (H ions) to be pumped out of the cell into the cell wall. As a result, the cell wall solution becomes more acidic. This activates expansin activity, causing the wall to become more extensible and to undergo wall stress relaxation, which enables the cell to take up water and to expand. Acid growth Acid growth refers to the ability of plant cells and plant cell walls to elongate or expand quickly at low (acidic) pH. This form of growth does not involve an increase in cell number. During acid growth, plant cells enlarge rapidly because the cell walls are
Plant tissue culture can often be used to generate a new plant on culture media given the required nutrients and plant hormones. Preparation of plant tissue for tissue culture is performed under aseptic conditions under HEPA filtered air provided by a laminar flow cabinet. Thereafter, the tissue is grown in sterile containers, such as petri dishes or flasks in a growth room with controlled temperature and light intensity. Living plant materials from the environment are naturally contaminated on their surfaces (and sometimes interiors) with microorganisms, so their surfaces are sterilized in chemical solutions (usually alcohol and sodium or calcium hypochlorite) before suitable samples