Cultural eutrophication activity. Polar regions have less human activity and subsequently less cultural eutrophication. One response to added amounts of nutrients in the aquatic ecosystem is the rapid growth of microscopic algae, also known as an algal bloom. In freshwater systems, the formation of floating algal blooms are commonly nitrogen-fixing cyanobacteria (blue-green algae). This outcome is favoured when nitrogen inputs are reduced and phosphorus inputs are increased. Large amounts of algae reduce the amount of dissolved oxygen available in the water for other organisms, which increases fish mortality rates. These areas affected by algal blooms are known as dead zones, and are
Plant tissue culture can often be used to generate a new plant on culture media given the required nutrients and plant hormones. Preparation of plant tissue for tissue culture is performed under aseptic conditions under HEPA filtered air provided by a laminar flow cabinet. Thereafter, the tissue is grown in sterile containers, such as petri dishes or flasks in a growth room with controlled temperature and light intensity. Living plant materials from the environment are naturally contaminated on their surfaces (and sometimes interiors) with microorganisms, so their surfaces are sterilized in chemical solutions (usually alcohol and sodium or calcium hypochlorite) before suitable samples