Plant tolerance to herbivory affect plant growth and also tolerance are water, light, carbon dioxide and soil nutrients. Water and light levels are generally assumed to be positively associated with tolerance (Strauss and Agrawal 1999). However, there are exceptions such as evidence of decreased tolerance in "Madia sativa" with increased water availability (Wise and Abrahamson 2007, Gonzales "et al." 2008). Many studies have found CO levels to decrease tolerance in plants (Lau and Tiffin 2009). Increased nutrient levels are also commonly found to be negatively associated with tolerance (Wise and Abrahamson 2007). There are currently three prominent models that predict how resource levels may
Plant tissue culture can often be used to generate a new plant on culture media given the required nutrients and plant hormones. Preparation of plant tissue for tissue culture is performed under aseptic conditions under HEPA filtered air provided by a laminar flow cabinet. Thereafter, the tissue is grown in sterile containers, such as petri dishes or flasks in a growth room with controlled temperature and light intensity. Living plant materials from the environment are naturally contaminated on their surfaces (and sometimes interiors) with microorganisms, so their surfaces are sterilized in chemical solutions (usually alcohol and sodium or calcium hypochlorite) before suitable samples