Plant nutrition of nutrient-rich water is pumped through the system to provide nutrients and water to the plant. Plant nutrition Plant nutrition is the study of the chemical elements and compounds necessary for plant growth, plant metabolism and their external supply. In 1972, Emanuel Epstein defined two criteria for an element to be essential for plant growth: This is in accordance with Justus von Liebig's law of the minimum. The essential plant nutrients include carbon, oxygen and hydrogen which are absorbed from the air, whereas other nutrients including nitrogen are typically obtained from the soil (exceptions include some parasitic or carnivorous plants).
Plant tissue culture can often be used to generate a new plant on culture media given the required nutrients and plant hormones. Preparation of plant tissue for tissue culture is performed under aseptic conditions under HEPA filtered air provided by a laminar flow cabinet. Thereafter, the tissue is grown in sterile containers, such as petri dishes or flasks in a growth room with controlled temperature and light intensity. Living plant materials from the environment are naturally contaminated on their surfaces (and sometimes interiors) with microorganisms, so their surfaces are sterilized in chemical solutions (usually alcohol and sodium or calcium hypochlorite) before suitable samples