Nutrient cycling in the Columbia River Basin eutrophication in part due to the use of the river and its tributaries to irrigate over seven million acres of farmland, an extraction that comprises an average of 93 percent of daily water use in the basin. Natural run-off caused by precipitation and snowpack melt across agricultural lands further transports more of these nutrients into the river. In the late 1990s, the nitrogen load in the Columbia river was over twice the concentration historically observed. Forests are important terrestrial sinks for nutrients due to the ability of vegetation to sequester excess nutrients into their biomass. Additionally, plant roots stabilize soils
Plant defense against herbivory change their defenses in response to changes in nutrients. For example, if plants are grown in low-nitrogen conditions, then these plants will implement a defensive strategy composed of constitutive carbon-based defenses. If nutrient levels subsequently increase, by for example the addition of fertilizers, these carbon-based defenses will decrease. The growth rate hypothesis, also known as the "resource availability hypothesis", states that defense strategies are determined by the inherent growth rate of the plant, which is in turn determined by the resources available to the plant. A major assumption is that available resources are the limiting factor in determining the maximum