Lake Erie eutrophication, in which additional nitrogen and phosphorus enter the lake, will cause plant life to "run wild and multiply like crazy". Since there are fewer wetlands, which are like "Nature's kidneys" by filtering nutrients, as well as greater "channelization of waterways", nutrients in water can cause algal blooms to sprout as well as "low-oxygen dead zones" in a complex interaction of natural forces. As of the 2010s much of the phosphorus in the lake comes from fertilizer applied to no-till soybean and corn fields but washed into streams by heavy rains. The algal blooms result from growth of Microcystis, a
Plant tissue culture can often be used to generate a new plant on culture media given the required nutrients and plant hormones. Preparation of plant tissue for tissue culture is performed under aseptic conditions under HEPA filtered air provided by a laminar flow cabinet. Thereafter, the tissue is grown in sterile containers, such as petri dishes or flasks in a growth room with controlled temperature and light intensity. Living plant materials from the environment are naturally contaminated on their surfaces (and sometimes interiors) with microorganisms, so their surfaces are sterilized in chemical solutions (usually alcohol and sodium or calcium hypochlorite) before suitable samples