Water pollution Oxygen-depleting substances may be natural materials such as plant matter (e.g. leaves and grass) as well as man-made chemicals. Other natural and anthropogenic substances may cause turbidity (cloudiness) which blocks light and disrupts plant growth, and clogs the gills of some fish species. Alteration of water's physical chemistry includes acidity (change in pH), electrical conductivity, temperature, and eutrophication. Eutrophication is an increase in the concentration of chemical nutrients in an ecosystem to an extent that increases the primary productivity of the ecosystem. Depending on the degree of eutrophication, subsequent negative environmental effects such as anoxia (oxygen depletion) and severe reductions
Plant tolerance to herbivory Using simulated herbivory allows for the control of environmental variables, but replicating natural herbivory is difficult, causing plants to respond differently from imposed and natural herbivory (Tiffin and Inouye 2000). Growing plants in the control environment of the greenhouse may also affect their response as it is still a novel environment to the plants. Even if the plots are grown in natural settings, the methods of excluding or including herbivores, such as using cages or pesticides, may also affect plant tolerance (Tiffin and Inouye 2000). Lastly, models have predicted that manipulated herbivory may actually result in less precise estimates of