Pollution in the fashion industry impact on the environment, the natural crop growing fibers also have been contributing to pollution through the form of agriculture. Cotton production requires a large amount of pesticides and water use. Cotton is considered the world's dirtiest crop because it uses 16% of the world's pesticides. Two of the main ingredients in pesticides are nitrates and phosphates. When the pesticides leak into stream systems surrounding the cropland the nitrates and phosphates contribute to water eutrophication. Water eutrophication is an environmental phenomenon that causes a depletion of oxygen when the nutrient overload from pesticides causes a boom in plant growth and
Plant tissue culture can often be used to generate a new plant on culture media given the required nutrients and plant hormones. Preparation of plant tissue for tissue culture is performed under aseptic conditions under HEPA filtered air provided by a laminar flow cabinet. Thereafter, the tissue is grown in sterile containers, such as petri dishes or flasks in a growth room with controlled temperature and light intensity. Living plant materials from the environment are naturally contaminated on their surfaces (and sometimes interiors) with microorganisms, so their surfaces are sterilized in chemical solutions (usually alcohol and sodium or calcium hypochlorite) before suitable samples