Groundwater-dependent ecosystems face a greater threat of pollution. In many cases groundwater can become polluted through toxins, or even just excessive amounts of certain nutrients seeping down to the water table. This polluting of the groundwater can have many different effects on the related ecosystems in the case of an estuary in Cape Cod it was noted that an influx of new nitrogen had come from septic tank fields in the groundwater’s flow path. Increased levels of nitrogen in aquatic ecosystems can cause eutrophication which is the process of excessive introduction of nutrients causing an abundance of plant growth which can result
Plant tolerance to herbivory Using simulated herbivory allows for the control of environmental variables, but replicating natural herbivory is difficult, causing plants to respond differently from imposed and natural herbivory (Tiffin and Inouye 2000). Growing plants in the control environment of the greenhouse may also affect their response as it is still a novel environment to the plants. Even if the plots are grown in natural settings, the methods of excluding or including herbivores, such as using cages or pesticides, may also affect plant tolerance (Tiffin and Inouye 2000). Lastly, models have predicted that manipulated herbivory may actually result in less precise estimates of