Polyculture chemicals into the environment such as eutrophication of fresh water or the presence of excess atmospheric nitrogen are greatly reduced in polycultures. Other negative impacts of modern agriculture are similarly reduced. Excessive tillage occurs in most modern agricultural practices, but removes essential microbes and nutrients from the soil that are conserved in polycultures, especially permacultures. Agricultural machinery not only uses fossil fuels and creates waste, but is more costly to farmers. Because polyculture relies on natural systems of crop maintenance these costs can be eliminated as well. Modern agriculture is also dependent on a very limited number of plant species.
Plant tolerance to herbivory Using simulated herbivory allows for the control of environmental variables, but replicating natural herbivory is difficult, causing plants to respond differently from imposed and natural herbivory (Tiffin and Inouye 2000). Growing plants in the control environment of the greenhouse may also affect their response as it is still a novel environment to the plants. Even if the plots are grown in natural settings, the methods of excluding or including herbivores, such as using cages or pesticides, may also affect plant tolerance (Tiffin and Inouye 2000). Lastly, models have predicted that manipulated herbivory may actually result in less precise estimates of