Sewage treatment to the environment can lead to a buildup of nutrients, called eutrophication, which can in turn encourage the overgrowth of weeds, algae, and cyanobacteria (blue-green algae). This may cause an algal bloom, a rapid growth in the population of algae. The algae numbers are unsustainable and eventually most of them die. The decomposition of the algae by bacteria uses up so much of the oxygen in the water that most or all of the animals die, which creates more organic matter for the bacteria to decompose. In addition to causing deoxygenation, some algal species produce toxins that contaminate drinking water
Plant tolerance to herbivory Using simulated herbivory allows for the control of environmental variables, but replicating natural herbivory is difficult, causing plants to respond differently from imposed and natural herbivory (Tiffin and Inouye 2000). Growing plants in the control environment of the greenhouse may also affect their response as it is still a novel environment to the plants. Even if the plots are grown in natural settings, the methods of excluding or including herbivores, such as using cages or pesticides, may also affect plant tolerance (Tiffin and Inouye 2000). Lastly, models have predicted that manipulated herbivory may actually result in less precise estimates of