Groundwater-dependent ecosystems face a greater threat of pollution. In many cases groundwater can become polluted through toxins, or even just excessive amounts of certain nutrients seeping down to the water table. This polluting of the groundwater can have many different effects on the related ecosystems in the case of an estuary in Cape Cod it was noted that an influx of new nitrogen had come from septic tank fields in the groundwater’s flow path. Increased levels of nitrogen in aquatic ecosystems can cause eutrophication which is the process of excessive introduction of nutrients causing an abundance of plant growth which can result
Plant tissue culture can often be used to generate a new plant on culture media given the required nutrients and plant hormones. Preparation of plant tissue for tissue culture is performed under aseptic conditions under HEPA filtered air provided by a laminar flow cabinet. Thereafter, the tissue is grown in sterile containers, such as petri dishes or flasks in a growth room with controlled temperature and light intensity. Living plant materials from the environment are naturally contaminated on their surfaces (and sometimes interiors) with microorganisms, so their surfaces are sterilized in chemical solutions (usually alcohol and sodium or calcium hypochlorite) before suitable samples