Eutrophication phosphate into water is slow, hence the difficulty of reversing the effects of eutrophication. However, numerous literature report that nitrogen is the primary limiting nutrient for the accumulation of algal biomass. The sources of these excess phosphates are phosphates in detergent, industrial/domestic run-offs, and fertilizers. With the phasing out of phosphate-containing detergents in the 1970s, industrial/domestic run-off and agriculture have emerged as the dominant contributors to eutrophication. Cultural eutrophication is the process that speeds up natural eutrophication because of human activity. Due to clearing of land and building of towns and cities, land runoff is accelerated and more nutrients such
Plant tissue culture can often be used to generate a new plant on culture media given the required nutrients and plant hormones. Preparation of plant tissue for tissue culture is performed under aseptic conditions under HEPA filtered air provided by a laminar flow cabinet. Thereafter, the tissue is grown in sterile containers, such as petri dishes or flasks in a growth room with controlled temperature and light intensity. Living plant materials from the environment are naturally contaminated on their surfaces (and sometimes interiors) with microorganisms, so their surfaces are sterilized in chemical solutions (usually alcohol and sodium or calcium hypochlorite) before suitable samples