Natural stress plant growth. When there is no water in the soil there are not very many nutrients to support plant growth. Drought also enhances the effects of wind. When drought occurs the soil becomes very dry and light. The wind picks up this dry dirt and carries it away. This action severely degrades the soil and creates a poor condition for growing plants. Plants have been exposed to the elements for thousands of years. During this time they have evolved in order to lessen the effects of abiotic stress. Signal transduction is the mechanism in plants that is responsible for the
Plant tissue culture can often be used to generate a new plant on culture media given the required nutrients and plant hormones. Preparation of plant tissue for tissue culture is performed under aseptic conditions under HEPA filtered air provided by a laminar flow cabinet. Thereafter, the tissue is grown in sterile containers, such as petri dishes or flasks in a growth room with controlled temperature and light intensity. Living plant materials from the environment are naturally contaminated on their surfaces (and sometimes interiors) with microorganisms, so their surfaces are sterilized in chemical solutions (usually alcohol and sodium or calcium hypochlorite) before suitable samples