Eutrophication die, the bacterial degradation of their biomass consumes the oxygen in the water, thereby creating the state of hypoxia. According to Ullmann's Encyclopedia, "the primary limiting factor for eutrophication is phosphate." The availability of phosphorus generally promotes excessive plant growth and decay, favouring simple algae and plankton over other more complicated plants, and causes a severe reduction in water quality. Phosphorus is a necessary nutrient for plants to live, and is the limiting factor for plant growth in many freshwater ecosystems. Phosphate adheres tightly to soil, so it is mainly transported by erosion. Once translocated to lakes, the extraction of
Plant tissue culture can often be used to generate a new plant on culture media given the required nutrients and plant hormones. Preparation of plant tissue for tissue culture is performed under aseptic conditions under HEPA filtered air provided by a laminar flow cabinet. Thereafter, the tissue is grown in sterile containers, such as petri dishes or flasks in a growth room with controlled temperature and light intensity. Living plant materials from the environment are naturally contaminated on their surfaces (and sometimes interiors) with microorganisms, so their surfaces are sterilized in chemical solutions (usually alcohol and sodium or calcium hypochlorite) before suitable samples