Hood Canal blooms occur in part because of warm weather and the slow turnover of water in the southern end of the canal, causing the build-up of nutrients from fertilizers and leaky septic systems. Organic matter, brought in by ocean water and certain trees, could additionally be contributing to the high nitrogen levels in the basin. Excess nutrients and organic matter causes a body of water to lose oxygen, through a process called eutrophication. In Hood Canal, eutrophication has led to unwanted algae blooms. Nitrogen combined with sunlight triggers algal growth. A lack of sufficient consumers has resulted in a mass overgrowth
Plant tissue culture can often be used to generate a new plant on culture media given the required nutrients and plant hormones. Preparation of plant tissue for tissue culture is performed under aseptic conditions under HEPA filtered air provided by a laminar flow cabinet. Thereafter, the tissue is grown in sterile containers, such as petri dishes or flasks in a growth room with controlled temperature and light intensity. Living plant materials from the environment are naturally contaminated on their surfaces (and sometimes interiors) with microorganisms, so their surfaces are sterilized in chemical solutions (usually alcohol and sodium or calcium hypochlorite) before suitable samples