Physiological plant disorder diseases. Improving drainage will help to alleviate this problem. Hail can cause damage to soft skinned fruits, and may also allow brown rot or other fungi to penetrate the plant. Brown spot markings or lines on one side of a mature apple are indicative of a spring hailstorm. Plants affected by salt stress are able to take water from soil, due to an osmotic imbalance between soil and plant. Poor growth and a variety of disorders such as leaf discolouration (chlorosis) can be caused by a shortage of one or more plant nutrients. Poor plant uptake of a nutrient from
Plant tissue culture can often be used to generate a new plant on culture media given the required nutrients and plant hormones. Preparation of plant tissue for tissue culture is performed under aseptic conditions under HEPA filtered air provided by a laminar flow cabinet. Thereafter, the tissue is grown in sterile containers, such as petri dishes or flasks in a growth room with controlled temperature and light intensity. Living plant materials from the environment are naturally contaminated on their surfaces (and sometimes interiors) with microorganisms, so their surfaces are sterilized in chemical solutions (usually alcohol and sodium or calcium hypochlorite) before suitable samples