Sodium triphosphate (due to hydrolysis), no studies have been carried out to date concerning the chronic effects of STPP on these aquatic organisms. Predicted no-effect concentrations were therefore calculated for the aquatic environment and sediments on the basis of the acute aquatic ecotoxicity results. Detergents containing phosphorus contribute, together with other sources of phosphorus, to the eutrophication of many fresh waters. Eutrophication is an increase in chemical nutrients—typically compounds containing nitrogen or phosphorus—in an ecosystem. It may occur on land or in water. The term is, however, often used to mean the resultant increase in the ecosystem's primary productivity (excessive plant growth
Plant tissue culture can often be used to generate a new plant on culture media given the required nutrients and plant hormones. Preparation of plant tissue for tissue culture is performed under aseptic conditions under HEPA filtered air provided by a laminar flow cabinet. Thereafter, the tissue is grown in sterile containers, such as petri dishes or flasks in a growth room with controlled temperature and light intensity. Living plant materials from the environment are naturally contaminated on their surfaces (and sometimes interiors) with microorganisms, so their surfaces are sterilized in chemical solutions (usually alcohol and sodium or calcium hypochlorite) before suitable samples