Cultural eutrophication found where rivers empty into oceans. Nutrient pollution is a major cause of algal blooming however, the excess nutrients also facilitate the growth of other aquatic plants. Following this, overcrowding occurs and plants compete for sunlight, space, and oxygen. Overgrowth of water plants also blocks sunlight and oxygen for aquatic life in the water, which threatens their survival. Increased competition for the added nutrients can cause potential disruption to entire ecosystems and food webs, as well as a loss of habitat, and biodiversity of species. The Experimental Lakes Area (ELA), Ontario, Canada is a fully equipped, year-round, permanent field station
Plant tissue culture can often be used to generate a new plant on culture media given the required nutrients and plant hormones. Preparation of plant tissue for tissue culture is performed under aseptic conditions under HEPA filtered air provided by a laminar flow cabinet. Thereafter, the tissue is grown in sterile containers, such as petri dishes or flasks in a growth room with controlled temperature and light intensity. Living plant materials from the environment are naturally contaminated on their surfaces (and sometimes interiors) with microorganisms, so their surfaces are sterilized in chemical solutions (usually alcohol and sodium or calcium hypochlorite) before suitable samples