Nonpoint source water pollution regulations in the United States The primary nutrients found in water due to nonpoint source pollution are nitrogen and phosphorus from fertilizers. An influx of these nutrients can lead to eutrophication within the water. This is when there is an excess of nutrients in water, thus leading to an explosion in the growth of algae. The algae can cover the lake and block sunlight from reaching the organisms below, typically killing them. NPS water pollution is regulated at the federal, state, and local level. Yet, the pollution persists and remains a significant problem. There are a number of reasons why current regulations have failed to
Plant tissue culture can often be used to generate a new plant on culture media given the required nutrients and plant hormones. Preparation of plant tissue for tissue culture is performed under aseptic conditions under HEPA filtered air provided by a laminar flow cabinet. Thereafter, the tissue is grown in sterile containers, such as petri dishes or flasks in a growth room with controlled temperature and light intensity. Living plant materials from the environment are naturally contaminated on their surfaces (and sometimes interiors) with microorganisms, so their surfaces are sterilized in chemical solutions (usually alcohol and sodium or calcium hypochlorite) before suitable samples