Polyculture chemicals into the environment such as eutrophication of fresh water or the presence of excess atmospheric nitrogen are greatly reduced in polycultures. Other negative impacts of modern agriculture are similarly reduced. Excessive tillage occurs in most modern agricultural practices, but removes essential microbes and nutrients from the soil that are conserved in polycultures, especially permacultures. Agricultural machinery not only uses fossil fuels and creates waste, but is more costly to farmers. Because polyculture relies on natural systems of crop maintenance these costs can be eliminated as well. Modern agriculture is also dependent on a very limited number of plant species.
Plant tissue culture can often be used to generate a new plant on culture media given the required nutrients and plant hormones. Preparation of plant tissue for tissue culture is performed under aseptic conditions under HEPA filtered air provided by a laminar flow cabinet. Thereafter, the tissue is grown in sterile containers, such as petri dishes or flasks in a growth room with controlled temperature and light intensity. Living plant materials from the environment are naturally contaminated on their surfaces (and sometimes interiors) with microorganisms, so their surfaces are sterilized in chemical solutions (usually alcohol and sodium or calcium hypochlorite) before suitable samples