PH the Hammett acidity function, "H", has been developed in connection with superacids. The concept of "unified pH scale" has been developed on the basis of the absolute chemical potential of the proton. This model uses the Lewis acid–base definition. This scale applies to liquids, gases and even solids. In 2010, a new "unified absolute pH scale" has been proposed that would allow various pH ranges across different solutions to use a common proton reference standard. Pure water is neutral. When an acid is dissolved in water, the pH will be less than 7 (25 °C). When a base, or alkali,
Gel electrophoresis of proteins the dye. The proteins are detected as blue bands on a clear background. When more sensitive method than staining by Coomassie is needed silver staining is usually used. Silver staining is a sensitive procedure to detect trace amounts of proteins in gels, but can also visualize nucleic acid or polysaccharides. Similarly as in nucleic acid gel electrophoresis, tracking dye is often used. Anionic dyes of a known electrophoretic mobility are usually included in the sample buffer. A very common tracking dye is Bromophenol blue. This dye is coloured at alkali and neutral pH and is a small negatively charged molecule