PH the Hammett acidity function, "H", has been developed in connection with superacids. The concept of "unified pH scale" has been developed on the basis of the absolute chemical potential of the proton. This model uses the Lewis acid–base definition. This scale applies to liquids, gases and even solids. In 2010, a new "unified absolute pH scale" has been proposed that would allow various pH ranges across different solutions to use a common proton reference standard. Pure water is neutral. When an acid is dissolved in water, the pH will be less than 7 (25 °C). When a base, or alkali,
Immobilized pH gradient gradient instability and poor sample loading capacity associated with carrier ampholyte pH gradient. Commercial precast IPG gels are available. Immobilized pH gradient Immobilized pH gradient (IPG) gels are the acrylamide gel matrix co-polymerized with the pH gradient, which result in completely stable gradients except the most alkaline (>12) pH values. The immobilized pH gradient is obtained by the continuous change in the ratio of "Immobilines". An "Immobiline" is a weak acid or base defined by its pK value. Immobilized pH gradients (IPG) are made by mixing two kinds of acrylamide mixture, one with Immobiline having acidic buffering property and other