PH of neutrality is not exactly 7 (25 °C), although this is a good approximation in most cases. Neutrality is defined as the condition where [H] = [OH] (or the activities are equal). Since self-ionization of water holds the product of these concentration [H]×[OH] = K, it can be seen that at neutrality [H] = [OH] = , or pH = pK/2. pK is approximately 14 but depends on ionic strength and temperature, and so the pH of neutrality does also. Pure water and a solution of NaCl in pure water are both neutral, since dissociation of water produces equal numbers
Amino acid acids is about 9.4). Because all amino acids contain amine and carboxylic acid functional groups, they share amphiprotic properties. Below pH 2.2, the predominant form will have a neutral carboxylic acid group and a positive α-ammonium ion (net charge +1), and above pH 9.4, a negative carboxylate and neutral α-amino group (net charge −1). But at pH between 2.2 and 9.4, an amino acid usually contains both a negative carboxylate and a positive α-ammonium group, as shown in structure (2) on the right, so has net zero charge. This molecular state is known as a zwitterion, from the German Zwitter