John Rylands manufacturers, and about the same time they erected a new spinning mill. The Ainsworth mills, near Bolton, and other factories were subsequently acquired. Joseph and Richard retired around 1839 and the death of their father in July 1847 made John Rylands sole proprietor. The business continued to expand and in 1849 a warehouse was opened in Wood Street, London. A great fire occurred at the Manchester warehouse in 1854 but the loss, although very large, was speedily repaired. By 1864 the warehouses were seven storeys high and extended all the way along New High Street (now High Street) in Manchester:
John Rylands other benefactions he established and maintained orphanages, homes for aged gentlewomen, a home of rest for ministers of slender means, and he provided a town hall, public baths, library and a coffeehouse in the town of Stretford, where he lived. He also built an institute for the benefit of the villagers of Havenstreet on the Isle of Wight, where Rylands passed some of his later years from 1882, having built a house named Longford there after his mainland estate. His donations to the poor of Rome were so generous as to induce the king to decorate him in 1880 with