PH is dissolved in water, the pH will be greater than 7. A solution of a strong acid, such as hydrochloric acid, at concentration 1 mol dm has a pH of 0. A solution of a strong alkali, such as sodium hydroxide, at concentration 1 mol dm, has a pH of 14. Thus, measured pH values will lie mostly in the range 0 to 14, though negative pH values and values above 14 are entirely possible. Since pH is a logarithmic scale, a difference of one pH unit is equivalent to a tenfold difference in hydrogen ion concentration. <br> The pH
Gel electrophoresis of proteins the dye. The proteins are detected as blue bands on a clear background. When more sensitive method than staining by Coomassie is needed silver staining is usually used. Silver staining is a sensitive procedure to detect trace amounts of proteins in gels, but can also visualize nucleic acid or polysaccharides. Similarly as in nucleic acid gel electrophoresis, tracking dye is often used. Anionic dyes of a known electrophoretic mobility are usually included in the sample buffer. A very common tracking dye is Bromophenol blue. This dye is coloured at alkali and neutral pH and is a small negatively charged molecule