Phytoene desaturase (lycopene-forming) Phytoene desaturase (lycopene-forming) Phytoene desaturase (lycopene-forming) (CrtI, "four-step phytoene desaturase") (, "15-cis-phytoene:acceptor oxidoreductase (lycopene-forming)") are enzymes found in archaea, bacteria and fungi that are involved in carotenoid biosynthesis. They catalyze the conversion of colorless 15-"cis"-phytoene into a bright red lycopene in a biochemical pathway called the poly-trans pathway. The same process in plants and cyanobacteria utilizes four separate enzymes in a poly-cis pathway. Bacterial phytoene desaturases were shown to require FAD as a cofactor for their function. During the chemical reaction in total four additional double bonds are introduced into phytoene: In 2000 it was discovered that the gene insertion
Pigment chemicals also have their own reflection and absorption patterns, which can affect the final spectrum. For example, in pigment/binder mixtures, individual rays of light may not encounter pigment molecules and may be reflected unchanged. These stray rays of source light make the mixture appear to have a less saturated color. Pure pigment allows very little white light to escape, producing a highly saturated color, while a small quantity of pigment mixed with a lot of white binder will appear unsaturated and pale due to incident white light escaping unchanged. Naturally occurring pigments such as ochres and iron oxides have been