Salicylaldehyde be prepared from the corresponding phenol by the Duff reaction, or by treatment with paraformaldehyde in the presence of magnesium chloride and a base. Salicylaldehyde was identified as a characteristic aroma component of buckwheat. It is also one of the components of castoreum, the exudate from the castor sacs of the mature North American beaver ("Castor canadensis") and the European beaver ("Castor fiber"), used in perfumery. Furthermore, salicylaldehyde occurs in the larval defensive secretions of several leaf beetle species that belong the subtribe Chrysomelina. An example for a leaf beetle species that produces salicylaldehyde is the red poplar leaf beetle
Ground substance as hyaluronic acid, heparan sulfate, dermatan sulfate, and chondroitin sulfate, proteoglycans which GAGs are bound to, and glycoproteins. Components of the ground substance are secreted by fibroblasts. Usually it is not visible on slides, because it is lost during staining in the preparation process. Link proteins such as vinculin, spectrin and actomyosin stabilize the proteoglycans and organize elastic fibers in the ECM. Changes in the density of ground substance can allow collagen fibers to form aberrant cross-links. Loose connective tissue is characterized by few fibers and cells, and a relatively large amount of ground substance. Dense connective tissue has a