PH temperature increases. Pure water is neutral, pH 7 at (25 °C), being neither an acid nor a base. Contrary to popular belief, the pH value can be less than 0 or greater than 14 for very strong acids and bases respectively. Measurements of pH are important in agronomy, medicine, chemistry, water treatment, and many other applications. The pH scale is traceable to a set of standard solutions whose pH is established by international agreement. Primary pH standard values are determined using a concentration cell with transference, by measuring the potential difference between a hydrogen electrode and a standard electrode such
Acid dissociation constant the added complication that the neutral molecule is subject to an internal acid–base equilibrium in which the basic amino group attracts and binds the proton from the acidic carboxyl group, forming a zwitterion. At pH less than about 5 both the carboxylate group and the amino group are protonated. As pH increases the acid dissociates according to At high pH a second dissociation may take place. Thus the zwitterion, , is amphoteric because it may either be protonated or deprotonated. The equilibrium constant "K" for a base is usually defined as the "association" constant for protonation of the base, B,