Acid-free paper Acid-free paper Acid-free paper is paper that if infused in water yields a neutral or basic pH (7 or slightly greater). It can be made from any cellulose fiber as long as the active acid pulp is eliminated during processing. It is also lignin- and sulfur-free. Acid-free paper addresses the problem of preserving documents and artwork for long periods. Paper made from wood-based pulp that has not had its lignin removed turns yellow, becomes brittle, and deteriorates over time. When exposed to light and/or heat, the molecules in the acidic paper will break down even faster. Acidic wood-pulp paper became
Gel electrophoresis of proteins the dye. The proteins are detected as blue bands on a clear background. When more sensitive method than staining by Coomassie is needed silver staining is usually used. Silver staining is a sensitive procedure to detect trace amounts of proteins in gels, but can also visualize nucleic acid or polysaccharides. Similarly as in nucleic acid gel electrophoresis, tracking dye is often used. Anionic dyes of a known electrophoretic mobility are usually included in the sample buffer. A very common tracking dye is Bromophenol blue. This dye is coloured at alkali and neutral pH and is a small negatively charged molecule