PH is dissolved in water, the pH will be greater than 7. A solution of a strong acid, such as hydrochloric acid, at concentration 1 mol dm has a pH of 0. A solution of a strong alkali, such as sodium hydroxide, at concentration 1 mol dm, has a pH of 14. Thus, measured pH values will lie mostly in the range 0 to 14, though negative pH values and values above 14 are entirely possible. Since pH is a logarithmic scale, a difference of one pH unit is equivalent to a tenfold difference in hydrogen ion concentration. <br> The pH
Immobilized pH gradient gradient instability and poor sample loading capacity associated with carrier ampholyte pH gradient. Commercial precast IPG gels are available. Immobilized pH gradient Immobilized pH gradient (IPG) gels are the acrylamide gel matrix co-polymerized with the pH gradient, which result in completely stable gradients except the most alkaline (>12) pH values. The immobilized pH gradient is obtained by the continuous change in the ratio of "Immobilines". An "Immobiline" is a weak acid or base defined by its pK value. Immobilized pH gradients (IPG) are made by mixing two kinds of acrylamide mixture, one with Immobiline having acidic buffering property and other