Biltong relatively mild concentration of coriander oil (1.6%). In the two strains that were not effectively killed, "Bacillus cereus" and "Enterococcus faecalis", the coriander oil reduced their growth significantly. The need for food preservation in the new colony was pressing. Building up herds of livestock took a long time, but with game in abundance in South Africa, traditional methods were called upon to preserve the meat of large African animals such as the eland in a warm climate. Iceboxes and refrigerators had not been invented yet. Biltong as it is today evolved from the dried meat carried by the wagon-travelling Voortrekkers,
Biltong in acidic conditions (pH less than 4.6), therefore the toxin will not be formed in acidic foods. The antimicrobial properties of certain spices have also been drawn upon since ancient times. The spices introduced to biltong by the Dutch include pepper, coriander, and cloves. In January 2017, a research group at the University of Beira Interior in Portugal published a study on the antimicrobial properties of coriander oil (coriander being one of the main spices in the most basic of biltong recipes) against 12 bacterial strains, and found that 10 of the 12 strains of bacteria were killed with a