Buster (comics) title character, whose strip usually appeared on the front cover, was Buster himself. He was originally billed as "Buster: Son of Andy Capp"; Andy Capp is the lead character of the eponymous "Daily Mirror" newspaper strip, and Buster wore a similar flat cap to reinforce the connection. In early issues Buster often referred to his father, and Andy was seen in the comic (attempting to find a gas leak in three frames of the 18 June 1960 issue; shown in two drawn photographs in the 2 July issue that same year, the first of which was displayed by Buster's mum
CAPP-Seq to quantify the previously identified mutations in the patient. CAPP-Seq can be applied to ctDNA from multiple blood samples at different time points in order to follow tumor evolution. A series of steps are involved in analysis of CAPP-Seq data from mutation detection to validation and open source software can do most of the analysis. After the first step of variant calling, germline and loss of heterozygosity (LOH) mutations are removed in CAPP-seq to reduce the background biases. Several statistical significance tests can be performed against background to all type of variant calling. For example, statistical significance of tumor-derived SNVs