Cold War tensions and the polio vaccine was the first polio vaccine to receive approval of the U.S. government, and was used until 1961 in America, when the Sabin vaccine was recommended to replace it. Albert Sabin, a virologist who publicly disagreed with Salk and his killed vaccine, worked on creating a vaccine with live, attenuated vaccines. Despite Cold War tensions, in January, 1956 Mikhail Chumakov, the director of Moscow's Polio Research Institute, his wife and colleague Marina Voroshilova, and Anatoli Smorodentsev, traveled to the U.S. in order to study the Salk vaccine and visited the laboratory of Albert Sabin. With the clearance of the FBI, Sabin
Smallpox vaccine sheep were used. In the late 1940s and early 1950s, Leslie Collier, an English microbiologist working at the Lister Institute of Preventive Medicine, developed a method for producing a heat-stable freeze-dried vaccine in powdered form. Collier added 0.5% phenol to the vaccine to reduce the number of bacterial contaminants but the key stage was to add 5% peptone to the liquid vaccine before it was dispensed into ampoules. This protected the virus during the freeze drying process. After drying the ampoules were sealed under nitrogen. Like other vaccines, once reconstituted it became ineffective after 1–2 days at ambient temperatures. However,