Andy Capp entitled "Andy Capp: The Game", was released for the Commodore 64 and ZX Spectrum in Europe and North America. Players had to borrow money in order to replenish Andy's alcohol supply while avoiding fights with either his wife Flo or the police. When the children's comic "Buster" was launched in 1960, its masthead character was entitled "Buster: Son of Andy Capp". Buster wore a cloth cap similar to Andy's until 1992, but the connection was not recognised in the parent strip and had limited development in the children's comic. Buster often referred to his father, and Andy was seen in
CAPP-Seq to quantify the previously identified mutations in the patient. CAPP-Seq can be applied to ctDNA from multiple blood samples at different time points in order to follow tumor evolution. A series of steps are involved in analysis of CAPP-Seq data from mutation detection to validation and open source software can do most of the analysis. After the first step of variant calling, germline and loss of heterozygosity (LOH) mutations are removed in CAPP-seq to reduce the background biases. Several statistical significance tests can be performed against background to all type of variant calling. For example, statistical significance of tumor-derived SNVs