Albert Sabin conducted at Johns Hopkins Hospital in 1992 when Sabin was 86. A year later, Sabin died in Washington, D.C., from heart failure. Sabin refused to patent his vaccine, waiving every commercial exploitation by pharmaceutical industries, so that the low price would guarantee a more extensive spread of the treatment. From the development of his vaccine Sabin did not gain a single dollar, and continued to live on his salary as a professor. Albert Sabin Albert Bruce Sabin (born Abram Saperstein; August 26, 1906 – March 3, 1993) was a Polish American medical researcher, best known for developing the oral polio
Smallpox vaccine sheep were used. In the late 1940s and early 1950s, Leslie Collier, an English microbiologist working at the Lister Institute of Preventive Medicine, developed a method for producing a heat-stable freeze-dried vaccine in powdered form. Collier added 0.5% phenol to the vaccine to reduce the number of bacterial contaminants but the key stage was to add 5% peptone to the liquid vaccine before it was dispensed into ampoules. This protected the virus during the freeze drying process. After drying the ampoules were sealed under nitrogen. Like other vaccines, once reconstituted it became ineffective after 1–2 days at ambient temperatures. However,