Digitalis purpurea detect DNA or RNA. It can easily be attached to nucleotides by chemical modifications. DIG molecules are often linked to uridine nucleotides; DIG-labeled uridine (DIG-U) can then be incorporated into RNA probes via "in vitro" transcription. Once hybridisation occurs "in situ", RNA probes with the incorporated DIG-U can be detected with anti-DIG antibodies conjugated to alkaline phosphatase. To reveal the hybridised transcripts, alkaline phosphatase can be reacted with a chromogen to produce a coloured precipitate. The colours of the petals of the "Digitalis purpurea" are known to be determined by at least three genes that interact with each other. The
Orazio Comes Orazio Comes Orazio Comes (11 November 1848 – 13 October 1917) was an Italian botanist. From 1906 to 1917 he directed the Agricultural Higher Institute of Portici, in which, thanks to Vincenzo de Cesati, he begun teaching since 1877 as assistant of Nicola Pedicino for the chair of botany, to whom he succeeded in 1880. In his works he studied the physiology and plant pathology of Neapolitan mushrooms and agricultural plants, such as beans and tobacco. From 1881, Comes began to deal with the cultivation of tobacco to improve industrial production and, starting from 1891, he emphasized the study and