Age determination in fish fish. Johannes Reibisch, working for the Commission of Scientific Investigation of German Seas at Kiel, attempted to use Hoffbauer’s techniques to age plaice ("Plueronectes platessa") but found it difficult to accurately discern annuli. He decided to study a different structure and in 1899 he published the first procedures using otoliths as an aging structure. A fellow scientist also with the German Commission at Kiel, Friedriche Heincke, also frustrated with difficult scale annuli, further studied other structures to age fish. He discovered annuli in the vertebrae, opercula, and pectoral girdle and published his findings in Heicke 1905. The works of Hoffbauer,
Flatfish be a valid species in its own right. Flatfishes are found in oceans worldwide, ranging from the Arctic, through the tropics, to Antarctica. Most species are found in depths between 0 and , but a few have been recorded from depths in excess of . None have been confirmed from the abyssal or hadal zones. An observation of a flatfish from the Bathyscaphe Trieste at the bottom of the Mariana Trench at a depth of almost has been questioned by fish experts, and recent authorities do not recognize it as valid. Among the deepwater species, "Symphurus thermophilus" lives in congregating