Digitalis purpurea detect DNA or RNA. It can easily be attached to nucleotides by chemical modifications. DIG molecules are often linked to uridine nucleotides; DIG-labeled uridine (DIG-U) can then be incorporated into RNA probes via "in vitro" transcription. Once hybridisation occurs "in situ", RNA probes with the incorporated DIG-U can be detected with anti-DIG antibodies conjugated to alkaline phosphatase. To reveal the hybridised transcripts, alkaline phosphatase can be reacted with a chromogen to produce a coloured precipitate. The colours of the petals of the "Digitalis purpurea" are known to be determined by at least three genes that interact with each other. The
Mountaineer Power Plant that coal now comes via barge on the Ohio River to an elaborate coal unloading and storage facility.The other portion of coal comes from Big River Mining. An underground coal mine right across the street from the plant. It is mined, cleaned, processed, and shipped by a belt line across the street to the power plant. This single boiler, dual turbine plant produces enough energy to power a city approximately the size of Columbus, Ohio. The plant is connected to the grid by 765 kilovolt transmission lines (the highest rated voltage used in the United States). The location of the