Digitalis purpurea detect DNA or RNA. It can easily be attached to nucleotides by chemical modifications. DIG molecules are often linked to uridine nucleotides; DIG-labeled uridine (DIG-U) can then be incorporated into RNA probes via "in vitro" transcription. Once hybridisation occurs "in situ", RNA probes with the incorporated DIG-U can be detected with anti-DIG antibodies conjugated to alkaline phosphatase. To reveal the hybridised transcripts, alkaline phosphatase can be reacted with a chromogen to produce a coloured precipitate. The colours of the petals of the "Digitalis purpurea" are known to be determined by at least three genes that interact with each other. The
Nothing comes from nothing a relationship between Maria's theological acumen and this song is accidental or intentional. In the web television series "13 Reasons Why", Ryan Shaver uses this Latin phrase to show Hannah Baker, the main character, that her attempts to write poetry will be useless if she's not willing to put her soul on them, as she is afraid of expressing her most intimate thoughts with strangers. The phrase also appears in H.P. Lovecraft's tale, "The Whisperer in Darkness". Nothing comes from nothing Nothing comes from nothing () is a philosophical expression of a thesis first argued by Parmenides. It is associated