The Ambassadors (Holbein) been hypothesized that the painting is meant to hang in a stairwell, so that persons walking up the stairs and passing the painting on their left would be startled by the appearance of the skull. A further possibility is that Holbein simply wished to show off his ability with the technique in order to secure future commissions. Artists often incorporated skulls as a reminder of mortality. Holbein may have intended the skulls (one as a gray slash and the other as a medallion on Jean de Dinteville's hat) and the crucifix in the upper left corner to encourage contemplation of
Hans Holbein the Younger including an anamorphic (distorted) skull. According to scholars, these are enigmatic references to learning, religion, mortality, and illusion in the tradition of the Northern Renaissance. Art historians Oskar Bätschmann and Pascal Griener suggest that, in "The Ambassadors", "Sciences and arts, objects of luxury and glory, are measured against the grandeur of Death". No certain portraits survive of Anne Boleyn by Holbein, perhaps because her memory was purged following her execution for treason, incest, and adultery in 1536. It is clear, however, that Holbein worked directly for Anne and her circle. He designed a cup engraved with her device of a