Age determination in fish fish. Johannes Reibisch, working for the Commission of Scientific Investigation of German Seas at Kiel, attempted to use Hoffbauer’s techniques to age plaice ("Plueronectes platessa") but found it difficult to accurately discern annuli. He decided to study a different structure and in 1899 he published the first procedures using otoliths as an aging structure. A fellow scientist also with the German Commission at Kiel, Friedriche Heincke, also frustrated with difficult scale annuli, further studied other structures to age fish. He discovered annuli in the vertebrae, opercula, and pectoral girdle and published his findings in Heicke 1905. The works of Hoffbauer,
Flatfish around "ponds" of sulphur at hydrothermal vents on the seafloor. No other flatfish is known from hydrothermal vents. Many species will enter brackish or fresh water, and a smaller number of soles (families Achiridae and Soleidae) and tonguefish (Cynoglossidae) are entirely restricted to fresh water. The most obvious characteristic of the flatfish is its asymmetry, with both eyes lying on the same side of the head in the adult fish. In some families, the eyes are usually on the right side of the body (dextral or right-eyed flatfish), and in others, they are usually on the left (sinistral or left-eyed