Digitalis by digitalis, therefore, the second-choice drug lidocaine is more commonly used. Digoxigenin (DIG) is a steroid found exclusively in the flowers and leaves of the plants "D. purpurea" and "D. lanata". It is used as a molecular probe to detect DNA or RNA. It can easily be attached to nucleotides by chemical modifications. DIG molecules are often linked to uridine nucleotides; DIG-labelled uridine (DIG-U) can then be incorporated into RNA probes via "in vitro" transcription. Once hybridisation occurs "in situ", RNA probes with the incorporated DIG-U can be detected with anti-DIG antibodies that are conjugated to alkaline phosphatase. To reveal
Nothing comes from nothing the song "Something Good", which contains the lyrics: "Nothing comes from nothing / nothing ever could". Although the song is devoid of intentional metaphysical connotations, Maria's time in Nonnberg Abbey would have familiarized her with the phrase, which is used in Catholic philosophy to explain God's gratuitous act of , namely, that only the source of all being can give creation its existence. Earlier in the film, in the well-known song "Maria", Maria's fellow nuns lament that there is "many a thing she ought to understand" and that she is "not an asset to the abbey". It is unclear if