Digitalis purpurea detect DNA or RNA. It can easily be attached to nucleotides by chemical modifications. DIG molecules are often linked to uridine nucleotides; DIG-labeled uridine (DIG-U) can then be incorporated into RNA probes via "in vitro" transcription. Once hybridisation occurs "in situ", RNA probes with the incorporated DIG-U can be detected with anti-DIG antibodies conjugated to alkaline phosphatase. To reveal the hybridised transcripts, alkaline phosphatase can be reacted with a chromogen to produce a coloured precipitate. The colours of the petals of the "Digitalis purpurea" are known to be determined by at least three genes that interact with each other. The
Nothing comes from nothing Nothing comes from nothing Nothing comes from nothing () is a philosophical expression of a thesis first argued by Parmenides. It is associated with ancient Greek cosmology, such as is presented not just in the works of Homer and Hesiod, but also in virtually every internal system—there is no break in-between a world that did not exist and one that did, since it could not be created in the first place. The idea that "nothing comes from nothing", as articulated by Parmenides, first appears in his "Physics": The above, in a translation based on the John Burnet translation, appears as