Digitalis thapsi than those found in "D. purpurea". According to Dutch botanist Herman Boerhaave, "Digitalis" species are highly poisonous if directly ingested. All parts are poisonous. Besides normal cultivation, "D. thapsi" has been propagated using explant culture, a technique employed for the isolation of cells from piece(s) of tissue for harvesting. Auxins NAA, 2,4-D and IAA alone or combined with BA produced a callus. NAA caused root formation and BA shoot formation. NAA and BA combined induced organ generation much effectively. Plantlets obtained this way had a survival rate of 70%. Joseph Gottlieb Kölreuter, a German botanist, observed that "D. thapsi" had
Orazio Comes Orazio Comes Orazio Comes (11 November 1848 – 13 October 1917) was an Italian botanist. From 1906 to 1917 he directed the Agricultural Higher Institute of Portici, in which, thanks to Vincenzo de Cesati, he begun teaching since 1877 as assistant of Nicola Pedicino for the chair of botany, to whom he succeeded in 1880. In his works he studied the physiology and plant pathology of Neapolitan mushrooms and agricultural plants, such as beans and tobacco. From 1881, Comes began to deal with the cultivation of tobacco to improve industrial production and, starting from 1891, he emphasized the study and