Ambrose Rookwood the House of Lords, Guy Fawkes, was discovered there and arrested. Rookwood fled the city, and informed Catesby and the others of the plan's failure. Together the remaining conspirators rode to Holbeche House in Staffordshire, where on 8 November they were attacked by the pursuing Sheriff of Worcester and his men. Catesby was killed, but Rookwood survived, and was imprisoned in the Tower of London. Rookwood and the survivors were arraigned on 27 January 1606 in Westminster Hall. Pleading not guilty, he claimed to have loved Catesby "above any worldly man". His subsequent request for mercy was ignored, however, and
Ambrose Rookwood daughter of William Tyrwhitt, of Kettleby in Lincolnshire, and his wife Elizabeth, daughter of Sir Peter Frescheville, of Staveley in Derbyshire. The Tyrwhitts were another prominent Roman Catholic family and his wife was possibly a first cousin to his future fellow conspirator Robert Keyes. The couple had two sons, Robert and Henry. On his father's death in 1600, he inherited Coldham Hall, which subsequently became a refuge for priests. The following year he joined the Earl of Essex's abortive rebellion against the government, for which he was captured and held at Newgate Prison. In August 1605 Rookwood joined the Jesuits