Two-hybrid screening developed in a silkworm cell line from the larva or caterpillar of the domesticated silk moth, "Bombyx mori" (BmN4 cells). This system uses the GAL4 BD and the activation domain of mouse NF-κB P65. Both are under the control of the OpIE2 promoter. By changing specific amino acids by mutating the corresponding DNA base-pairs in the plasmids used, the importance of those amino acid residues in maintaining the interaction can be determined. After using bacterial cell-based method to select DNA-binding proteins, it is necessary to check the specificity of these domains as there is a limit to the extent to
Bombyx hybrid have to be kept in the same container. Since "Bombyx" hybrids are much more common than the other variation more is known about them. The domesticated silkworm ("B. mori") was domesticated from wild silkworm ("B. mandarina") more than 5,000 years ago. Bombyx hybrid The "Bombyx" hybrid is a hybrid between a female "Bombyx mori" moth and a male "Bombyx mandarina" moth. They produce silkworm larvae like all species of "Bombyx". The larvae look a lot like the other variations. They are brown in the first half and gray at the bottom half, but they get larger black spots than other