Two-hybrid screening developed in a silkworm cell line from the larva or caterpillar of the domesticated silk moth, "Bombyx mori" (BmN4 cells). This system uses the GAL4 BD and the activation domain of mouse NF-κB P65. Both are under the control of the OpIE2 promoter. By changing specific amino acids by mutating the corresponding DNA base-pairs in the plasmids used, the importance of those amino acid residues in maintaining the interaction can be determined. After using bacterial cell-based method to select DNA-binding proteins, it is necessary to check the specificity of these domains as there is a limit to the extent to
Crustacean larva (lecithotrophy). In species with normal development, eggs are roughly 1% of the size of the adult; in species with abbreviated development, and therefore more yolk in the eggs, the eggs may reach 1/9 of the adult's size. The post-larva of shrimp is called "parva", after the species "Acanthephyra parva" described by Henri Coutière, but which was later recognised as the larva of "Acanthephyra purpurea". In the marine lobsters, there are three larval stages, all similar in appearance. Freshwater crayfish embryos differ from those of other crustaceans in having 40 ectoteloblast cells, rather than around 19. The larvae show abbreviated development,