Two-hybrid screening developed in a silkworm cell line from the larva or caterpillar of the domesticated silk moth, "Bombyx mori" (BmN4 cells). This system uses the GAL4 BD and the activation domain of mouse NF-κB P65. Both are under the control of the OpIE2 promoter. By changing specific amino acids by mutating the corresponding DNA base-pairs in the plasmids used, the importance of those amino acid residues in maintaining the interaction can be determined. After using bacterial cell-based method to select DNA-binding proteins, it is necessary to check the specificity of these domains as there is a limit to the extent to
Akira Mori Akira Mori He attended Keio University, graduating in 1960 and joining Yasuda Trust & Banking (now part of Mizuho Trust & Banking). He formally joined Mori Building as an employee in 1972. Following their father's death in 1993, he and his older brother Minoru Mori inherited the family business and in 1999 they split it after a falling-out. Akira headed Mori Trust, while his brother headed what was left of the Mori Building company until his death on March 8, 2012. Mori Trust owns and operates 67 buildings, manages 89 buildings, consisting of office and residential buildings, mainly in central