Two-hybrid screening developed in a silkworm cell line from the larva or caterpillar of the domesticated silk moth, "Bombyx mori" (BmN4 cells). This system uses the GAL4 BD and the activation domain of mouse NF-κB P65. Both are under the control of the OpIE2 promoter. By changing specific amino acids by mutating the corresponding DNA base-pairs in the plasmids used, the importance of those amino acid residues in maintaining the interaction can be determined. After using bacterial cell-based method to select DNA-binding proteins, it is necessary to check the specificity of these domains as there is a limit to the extent to
Toshia Mori film is presumed lost.) Finally, she entered the sound era as Toshia Mori. Mori played Miss Ling, in "The Hatchet Man" (1932). In the same year, she played another Chinese character, "Butterfly", in "Roar of the Dragon", an action-melodrama produced by David O. Selznick. The storyline consisted of a group of Occidentals turning to an alcoholic riverboat captain Chauncey Carson (Richard Dix) for help when they are trapped at a hotel in a Mandarin town under siege. In 1932, Toshia became the only Asian and non-Caucasian actress to be selected as a WAMPAS Baby Star, an annual list of young