Two-hybrid screening developed in a silkworm cell line from the larva or caterpillar of the domesticated silk moth, "Bombyx mori" (BmN4 cells). This system uses the GAL4 BD and the activation domain of mouse NF-κB P65. Both are under the control of the OpIE2 promoter. By changing specific amino acids by mutating the corresponding DNA base-pairs in the plasmids used, the importance of those amino acid residues in maintaining the interaction can be determined. After using bacterial cell-based method to select DNA-binding proteins, it is necessary to check the specificity of these domains as there is a limit to the extent to
Hiroshi Mori (writer) In fact, he finished his first novel in just a week by sparing a mere three hours at night after a day's work at the university. Although he writes very fast, he admits that he is not a good reader, and curiously, the bulk of his time is spent reading proof sheets rather than actually writing. At the same time, Mori is extremely punctual, and his editor Misa Inako affirms that he has never missed a deadline. Mori has so far produced over thirty mystery novels; moreover, he has worked on a wide variety of genres in the past few