Two-hybrid screening developed in a silkworm cell line from the larva or caterpillar of the domesticated silk moth, "Bombyx mori" (BmN4 cells). This system uses the GAL4 BD and the activation domain of mouse NF-κB P65. Both are under the control of the OpIE2 promoter. By changing specific amino acids by mutating the corresponding DNA base-pairs in the plasmids used, the importance of those amino acid residues in maintaining the interaction can be determined. After using bacterial cell-based method to select DNA-binding proteins, it is necessary to check the specificity of these domains as there is a limit to the extent to
Nicolas Mori programmes were headed by the grim device of a death's head and the legend Memento Mori. As a performer 'Mori's attitude had the grace of manly confidence. His bow arm was bold, free, and commanding, and the tone he produced was eminently firm, full, and impressive. His execution was alike marked by abundant force and fire, by extraordinary precision and prodigious facility, but lacked niceties of finish and the graces and delicacies of expression' (Quarterly Mag. Music, iii. 323). He left behind him a son, Francis Mori (1820-1873), the composer of a cantata, entitled 'Fridolin;' an operetta, with words by