Two-hybrid screening developed in a silkworm cell line from the larva or caterpillar of the domesticated silk moth, "Bombyx mori" (BmN4 cells). This system uses the GAL4 BD and the activation domain of mouse NF-κB P65. Both are under the control of the OpIE2 promoter. By changing specific amino acids by mutating the corresponding DNA base-pairs in the plasmids used, the importance of those amino acid residues in maintaining the interaction can be determined. After using bacterial cell-based method to select DNA-binding proteins, it is necessary to check the specificity of these domains as there is a limit to the extent to
Memento Mori (The X-Files) Memento Mori (The X-Files) "Memento Mori" is the fourteenth episode of the fourth season of the American science fiction television series "The X-Files". It premiered on the Fox network on . It was directed by Rob Bowman, and written by series creator Chris Carter, Vince Gilligan, John Shiban and Frank Spotnitz. "Memento Mori" featured guest appearances by Sheila Larken, David Lovgren and Morris Panych. The episode helped to explore the overarching mythology, or fictional history of "The X-Files". "Memento Mori" earned a Nielsen household rating of 15.5, being watched by 19.1 million people in its initial broadcast. The title translates