Two-hybrid screening developed in a silkworm cell line from the larva or caterpillar of the domesticated silk moth, "Bombyx mori" (BmN4 cells). This system uses the GAL4 BD and the activation domain of mouse NF-κB P65. Both are under the control of the OpIE2 promoter. By changing specific amino acids by mutating the corresponding DNA base-pairs in the plasmids used, the importance of those amino acid residues in maintaining the interaction can be determined. After using bacterial cell-based method to select DNA-binding proteins, it is necessary to check the specificity of these domains as there is a limit to the extent to
Crustacean larva post-larva is characterised by the use of abdominal appendages (pleopods) for propulsion. The post-larva is usually similar to the adult form, and so many names have been erected for the stage in different groups. William Elford Leach erected the genus "Megalopa" in 1813 for a post-larval crab; a shrimp post-larva is called a "parva"; hermit crab post-larva are called "glaucothoe". In the Branchiopoda, the most basal group of crustaceans, there is no metamorphosis; instead, the animal grows through a series of moults, with each moult adding various numbers of segments to the body, but without any dramatic changes in form.