Two-hybrid screening developed in a silkworm cell line from the larva or caterpillar of the domesticated silk moth, "Bombyx mori" (BmN4 cells). This system uses the GAL4 BD and the activation domain of mouse NF-κB P65. Both are under the control of the OpIE2 promoter. By changing specific amino acids by mutating the corresponding DNA base-pairs in the plasmids used, the importance of those amino acid residues in maintaining the interaction can be determined. After using bacterial cell-based method to select DNA-binding proteins, it is necessary to check the specificity of these domains as there is a limit to the extent to
Bárbara Mori allegedly saw in her nightmares. The crime-drama was filmed in Mexico city and is produced by Mori. The film earned mixed reviews from critics and viewers. In 2011, she got the main role in the action-drama ""Viento En Contra"", alongside actors Héctor Arredondo and Fernando Luján, in which she played a successful and promising CIO, who is unjustly involved in a police chase due to a scandal of a financial fraud. The film was distributed by Warner Bros and received mixed reviews by critics. The drama was filmed on locations of Mexico city and Valle de Bravo. Mori was the