Two-hybrid screening developed in a silkworm cell line from the larva or caterpillar of the domesticated silk moth, "Bombyx mori" (BmN4 cells). This system uses the GAL4 BD and the activation domain of mouse NF-κB P65. Both are under the control of the OpIE2 promoter. By changing specific amino acids by mutating the corresponding DNA base-pairs in the plasmids used, the importance of those amino acid residues in maintaining the interaction can be determined. After using bacterial cell-based method to select DNA-binding proteins, it is necessary to check the specificity of these domains as there is a limit to the extent to
Bombyx mandarina Bombyx mandarina Bombyx mandarina, the wild silkmoth, is an insect from the moth family Bombycidae. It is the closest relative of "Bombyx mori" the domesticated silkmoth or "silkworm" (properly, this refers to the caterpillars only). Unlike the domesticated relative which is unable to fly or indeed persist outside human care, the wild silkmoth is a fairly ordinary lepidopteran. Its main difference from the domesticated taxon is the more slender body with well-developed wings in males, and the dull greyish-brown colour. "Bombyx mandarina" and the domesticated "Bombyx mori" constitute two of the currently identified eight species of the genus "Bombyx", the