Two-hybrid screening developed in a silkworm cell line from the larva or caterpillar of the domesticated silk moth, "Bombyx mori" (BmN4 cells). This system uses the GAL4 BD and the activation domain of mouse NF-κB P65. Both are under the control of the OpIE2 promoter. By changing specific amino acids by mutating the corresponding DNA base-pairs in the plasmids used, the importance of those amino acid residues in maintaining the interaction can be determined. After using bacterial cell-based method to select DNA-binding proteins, it is necessary to check the specificity of these domains as there is a limit to the extent to
Cormoran Strike without trace. Quine, once hailed as a literary rebel —has struggled for years to recreate the success of his original novel and has fallen out of public favour. Strike discovers that his disappearance coincides with the leak of a manuscript for his latest novel, "Bombyx Mori". The London literary community considers "Bombyx Mori" to be unpublishable; an unpleasant mix of rape, sadomasochism, torture, necrophilia and cannibalism, the hero is eventually tricked and eaten alive by various characters who are thinly-veiled caricatures of people in Quine's life whom he considers responsible for the destruction of his career. The investigation soon takes