Two-hybrid screening developed in a silkworm cell line from the larva or caterpillar of the domesticated silk moth, "Bombyx mori" (BmN4 cells). This system uses the GAL4 BD and the activation domain of mouse NF-κB P65. Both are under the control of the OpIE2 promoter. By changing specific amino acids by mutating the corresponding DNA base-pairs in the plasmids used, the importance of those amino acid residues in maintaining the interaction can be determined. After using bacterial cell-based method to select DNA-binding proteins, it is necessary to check the specificity of these domains as there is a limit to the extent to
Mark Mori Mark Mori Mark Mori is an American documentary filmmaker, television producer and screenwriter of documentary and reality television series and specials. He produced and directed, "Bettie Page Reveals All", the authorized documentary film on life of pin-up model, Bettie Page, released in 2012. Previously, Mori produced and directed "Building Bombs", Academy Award nominee for Best Feature Documentary for 1990 (DVD release by Docurama/New Video in 2005), Executive Produced, "", documentary short Academy Award nominee for 1993; received an Emmy Award for the television documentary special, "Kent State, the Day the War Came" Home for 2000; co-produced "The Fire This Time",