Two-hybrid screening developed in a silkworm cell line from the larva or caterpillar of the domesticated silk moth, "Bombyx mori" (BmN4 cells). This system uses the GAL4 BD and the activation domain of mouse NF-κB P65. Both are under the control of the OpIE2 promoter. By changing specific amino acids by mutating the corresponding DNA base-pairs in the plasmids used, the importance of those amino acid residues in maintaining the interaction can be determined. After using bacterial cell-based method to select DNA-binding proteins, it is necessary to check the specificity of these domains as there is a limit to the extent to
Mori language Mori language In its broad sense, Mori is a cover term used to refer collectively to two languages of Central Sulawesi: Mori Bawah and Mori Atas. Sometimes a third language, Padoe, is also included. Originally the term 'Mori' referred only to certain clans living on the upper course of the Laa River in Central Sulawesi, that is, today's Mori Atas or 'Upper Mori' people. After the imposition of Dutch colonial rule in the early twentieth century, the name was extended to include peoples living eastward along the lower course of the Laa and in the Tambalako watershed (today's Mori Bawah