Two-hybrid screening developed in a silkworm cell line from the larva or caterpillar of the domesticated silk moth, "Bombyx mori" (BmN4 cells). This system uses the GAL4 BD and the activation domain of mouse NF-κB P65. Both are under the control of the OpIE2 promoter. By changing specific amino acids by mutating the corresponding DNA base-pairs in the plasmids used, the importance of those amino acid residues in maintaining the interaction can be determined. After using bacterial cell-based method to select DNA-binding proteins, it is necessary to check the specificity of these domains as there is a limit to the extent to
Müller's larva described larval forms of many phyla. Müller's larva Müller's larva or Mulleria is a larva of some Polycladida. It has 8-fold symmetry and is somewhat like a ctenophore. Müller’s larva is ciliated and has several paired and unpaired lobes. The cilia on the lobes are longer than cilia on the rest of the body. At the anterior and posterior ends of the larva are tufts of longer cilia (apical and caudal). The apical tuft originates from the apical organ, a sensory structure associated with the central nervous system. It is named after Johannes Peter Müller (1801-1858), a German physiologist who