Two-hybrid screening developed in a silkworm cell line from the larva or caterpillar of the domesticated silk moth, "Bombyx mori" (BmN4 cells). This system uses the GAL4 BD and the activation domain of mouse NF-κB P65. Both are under the control of the OpIE2 promoter. By changing specific amino acids by mutating the corresponding DNA base-pairs in the plasmids used, the importance of those amino acid residues in maintaining the interaction can be determined. After using bacterial cell-based method to select DNA-binding proteins, it is necessary to check the specificity of these domains as there is a limit to the extent to
Maru Maru Mori Mori! full-length download to cellphones. Sankei Sports reported that "Maru Maru Mori Mori!" crossed the 250 thousand album sales mark as of 17 July 2011. This makes the group the first Japanese group under 10 years old to exceed 200 thousand sales mark in over 35 years, the last person to do so being Kawahashi Hiroshi with his single "Yamaguchi-sanchi no Tsutomu-kun". On 16 August 2011, Oricon reported that this singles managed to remain in the top 10 list for 12 weeks in a row. Amongst singles released by an artist under 10 years old, "Maru Maru Mori Mori!" now holds