Two-hybrid screening developed in a silkworm cell line from the larva or caterpillar of the domesticated silk moth, "Bombyx mori" (BmN4 cells). This system uses the GAL4 BD and the activation domain of mouse NF-κB P65. Both are under the control of the OpIE2 promoter. By changing specific amino acids by mutating the corresponding DNA base-pairs in the plasmids used, the importance of those amino acid residues in maintaining the interaction can be determined. After using bacterial cell-based method to select DNA-binding proteins, it is necessary to check the specificity of these domains as there is a limit to the extent to
Centro Mori Centro Mori The Centro Mori or Centro Cultural Mori ("Mori Cultural Center") is a cultural project founded in Santiago, Chile by actors Gonzalo Valenzuela and Benjamín Vicuña, together with their partner Cristóbal Vial. The center is located in Barrio Bellavista - a neighborhood of Santiago with a strong cultural heritage - in an old house at the foot of San Cristóbal Hill (street address Constitución 183), next to the Plaza Camilo Mori and near "La Chascona" (the museum and former house of Pablo Neruda). The property was restored in 2005 by the actors and a group of professionals to turn