Two-hybrid screening developed in a silkworm cell line from the larva or caterpillar of the domesticated silk moth, "Bombyx mori" (BmN4 cells). This system uses the GAL4 BD and the activation domain of mouse NF-κB P65. Both are under the control of the OpIE2 promoter. By changing specific amino acids by mutating the corresponding DNA base-pairs in the plasmids used, the importance of those amino acid residues in maintaining the interaction can be determined. After using bacterial cell-based method to select DNA-binding proteins, it is necessary to check the specificity of these domains as there is a limit to the extent to
Bárbara Mori Bárbara Mori Bárbara Mori Ochoa (; born 2 February 1978) is an Uruguayan-born Mexican actress, model, producer and writer who started her career in 1992 as a fashion model at the age of 14, later she became an actress when she co-starred in 1997 on the smash TV-hit "Mirada De Mujer" with TV Azteca; then, she starred in the soap opera Azul Tequila. Her big break came in 2004, with the telenovela "Rubí" (a remake of the 1968 series) by rival network Televisa, in which she played the main character. She also has starred in several self-produced films and music