Two-hybrid screening developed in a silkworm cell line from the larva or caterpillar of the domesticated silk moth, "Bombyx mori" (BmN4 cells). This system uses the GAL4 BD and the activation domain of mouse NF-κB P65. Both are under the control of the OpIE2 promoter. By changing specific amino acids by mutating the corresponding DNA base-pairs in the plasmids used, the importance of those amino acid residues in maintaining the interaction can be determined. After using bacterial cell-based method to select DNA-binding proteins, it is necessary to check the specificity of these domains as there is a limit to the extent to
Mori (restaurant) survived the Prohibition era and the worst years of the Great Depression, when it was temporarily padlocked. Mori closed in 1937, and Placido Mori filed a petition for bankruptcy in early January 1938, stating that the corporation had no assets and liabilities totaling $70,000. The building formerly occupied by Mori was sold by Caroline Bussing through A.Q. Orza, broker, in October 1943. Mori's gravesite in Woodlawn Cemetery in the Bronx is marked with a sculpted memorial designed by Hood and sculptor Charles Keck. Mori (restaurant) Mori (1883 or 1884 – 1937 or 1938) was a Greenwich Village eating establishment that