Two-hybrid screening developed in a silkworm cell line from the larva or caterpillar of the domesticated silk moth, "Bombyx mori" (BmN4 cells). This system uses the GAL4 BD and the activation domain of mouse NF-κB P65. Both are under the control of the OpIE2 promoter. By changing specific amino acids by mutating the corresponding DNA base-pairs in the plasmids used, the importance of those amino acid residues in maintaining the interaction can be determined. After using bacterial cell-based method to select DNA-binding proteins, it is necessary to check the specificity of these domains as there is a limit to the extent to
Mori Bawah language Mori Bawah language Mori Bawah, also known as Lower Mori or East Mori, is an Austronesian language of the Celebic branch. It is one of the principal languages of the Morowali Regency in Central Sulawesi. Mori Bawah is classified as a member of the Bungku-Tolaki group of languages, and shares its closest affinities with Bungku and other languages of the eastern seaboard of Sulawesi, such as Wawonii and Kulisusu. Together, Mori Bawah and the Mori Atas language are sometimes referred to collectively by the cover term ‘Mori’. Mori Bawah comprises several dialects. Following Esser, five dialects can be regarded as