Orange (fruit) orange juice between the São Paulo and mid-south Florida areas makes up roughly 85% of the world market. Brazil exports 99% of its production, while 90% of Florida's production is consumed in the United States. Orange juice is traded internationally in the form of frozen, concentrated orange juice to reduce the volume used so that storage and transportation costs are lower. Oranges, whose flavor may vary from sweet to sour, are commonly peeled and eaten fresh or squeezed for juice. The thick bitter rind is usually discarded, but can be processed into animal feed by desiccation, using pressure and heat.
Orange juice d-galacturonic acid unit along with blocks of L-rhamnose regions that have side chains of arabinose, galactose, and xylose. Pectin methyl-esterase is the enzyme responsible for hydrolyzing carboxymethyl esters and liberating free carboxyl groups and methyl alcohols. The free carboxyl groups interact with cations to form insoluble pectic acid divalent metal ion complexes. These metal ion complexes precipitate in the juice and carry all the colloids in orange juice with it. The enzyme would flocculate the cloud and clarify the orange juice. Thus, in order to keep the orange juice cloud intact, it is vital to inactivate pectinesterase. Pectinesterase is inactivated