Two-hybrid screening developed in a silkworm cell line from the larva or caterpillar of the domesticated silk moth, "Bombyx mori" (BmN4 cells). This system uses the GAL4 BD and the activation domain of mouse NF-κB P65. Both are under the control of the OpIE2 promoter. By changing specific amino acids by mutating the corresponding DNA base-pairs in the plasmids used, the importance of those amino acid residues in maintaining the interaction can be determined. After using bacterial cell-based method to select DNA-binding proteins, it is necessary to check the specificity of these domains as there is a limit to the extent to
Mori Point Native Americans and, later, by the Spanish. Francisco Sanchez received "Rancho San Pedro" in 1839 during the Rancho period, which encompassed much of present-day Pacifica, including the land that would later be called Mori Point. The land now occupied by Mori Point was purchased by Stefano Mori and his family in 1888, who operated a farm and ranch at the point. The kitchen, built to feed employees, eventually became the Mori Point Inn, which remained in operation until 1965. The exact date of establishment is in question, but newspaper reports from 1910 cite Steve Mori as manager of the Inn