Two-hybrid screening developed in a silkworm cell line from the larva or caterpillar of the domesticated silk moth, "Bombyx mori" (BmN4 cells). This system uses the GAL4 BD and the activation domain of mouse NF-κB P65. Both are under the control of the OpIE2 promoter. By changing specific amino acids by mutating the corresponding DNA base-pairs in the plasmids used, the importance of those amino acid residues in maintaining the interaction can be determined. After using bacterial cell-based method to select DNA-binding proteins, it is necessary to check the specificity of these domains as there is a limit to the extent to
Dōbutsu no Mori (film) of approximately ¥1.7 billion ($16,216,731) by the end of its theatrical run in 2007, making it the 17th highest-grossing film of that year when combining it with its December 2006 box office total. "Dōbutsu no Mori" was released on region 2 DVD in Japan on July 25, 2007 by VAP. First-print copies also included an "Animal Crossing"-themed carrying pouch. Dōbutsu no Mori (film) , also known as Animal Crossing: The Movie, is a 2006 Japanese animated film directed by Jōji Shimura and based on the "Animal Crossing" video game series. It was produced by Nintendo, OLM, Inc. and VAP and