Two-hybrid screening developed in a silkworm cell line from the larva or caterpillar of the domesticated silk moth, "Bombyx mori" (BmN4 cells). This system uses the GAL4 BD and the activation domain of mouse NF-κB P65. Both are under the control of the OpIE2 promoter. By changing specific amino acids by mutating the corresponding DNA base-pairs in the plasmids used, the importance of those amino acid residues in maintaining the interaction can be determined. After using bacterial cell-based method to select DNA-binding proteins, it is necessary to check the specificity of these domains as there is a limit to the extent to
Mori Shogunzuka Kofun Mori Shogunzuka Kofun The is a keyhole-shaped tumulus within the located in Chikuma, Nagano Prefecture Japan. The "kofun" has been restored to what modern archaeologists believe to represent its original form, with reproduction haniwa on top of the mound. Its burial chamber is closed and inaccessible to the public. There is also a museum on site, the Mori Shogunzuka Museum, which displays a reproduction of the burial chamber as well as artifacts unearthed during archaeological investigations. The cluster of four tombs has been designated a National Historic Site by the Japanese government. The Mori Shogunzuka Kofun measures approximately 100 meters