Two-hybrid screening developed in a silkworm cell line from the larva or caterpillar of the domesticated silk moth, "Bombyx mori" (BmN4 cells). This system uses the GAL4 BD and the activation domain of mouse NF-κB P65. Both are under the control of the OpIE2 promoter. By changing specific amino acids by mutating the corresponding DNA base-pairs in the plasmids used, the importance of those amino acid residues in maintaining the interaction can be determined. After using bacterial cell-based method to select DNA-binding proteins, it is necessary to check the specificity of these domains as there is a limit to the extent to
Crustacean larva long rostrum, and a spine on either side of the carapace; the first post-larva closely resembles the adult. Porcelain crabs have two or three larval stages, in which the rostrum and the posterior spine on the carapace are "enormously long". Hermit crabs pass through around four larval stages. The post-larva is known as the "glaucothoe", after a genus named by Henri Milne-Edwards in 1830. The glaucothoe is long in "Pagurus longicarpus", but glaucothoe larvae up to are known, and were once thought to represent animals which had failed to develop correctly. Like the preceding stages, the glaucothoe is symmetrical, and