Two-hybrid screening developed in a silkworm cell line from the larva or caterpillar of the domesticated silk moth, "Bombyx mori" (BmN4 cells). This system uses the GAL4 BD and the activation domain of mouse NF-κB P65. Both are under the control of the OpIE2 promoter. By changing specific amino acids by mutating the corresponding DNA base-pairs in the plasmids used, the importance of those amino acid residues in maintaining the interaction can be determined. After using bacterial cell-based method to select DNA-binding proteins, it is necessary to check the specificity of these domains as there is a limit to the extent to
Maru Maru Mori Mori! the record of the longest streak on the Oricon charts. The previous record set by the 2007 song "Gake no Ue no Ponyo", the theme song of the Studio Ghibli film "Ponyo", which remained on the charts for 11 consecutive weeks in 2008. "Gake no Ue no Ponyo" featured the then 8-year old actress Nozomi Ōhashi. The song "Maru Maru Mori Mori" received the Special Award at the 53rd Japan Record Awards. Maru Maru Mori Mori! On 4 April 2011, it was announced that the theme song of the Fuji Television television drama "Marumo no Okite" will be sung by