Two-hybrid screening developed in a silkworm cell line from the larva or caterpillar of the domesticated silk moth, "Bombyx mori" (BmN4 cells). This system uses the GAL4 BD and the activation domain of mouse NF-κB P65. Both are under the control of the OpIE2 promoter. By changing specific amino acids by mutating the corresponding DNA base-pairs in the plasmids used, the importance of those amino acid residues in maintaining the interaction can be determined. After using bacterial cell-based method to select DNA-binding proteins, it is necessary to check the specificity of these domains as there is a limit to the extent to
Ocular larva migrans Ocular larva migrans Ocular larva migrans (OLM), also known as ocular toxocariasis, is the ocular form of the larva migrans syndrome that occurs when "Toxocara canis" (dog roundworm) larvae invade the eye. They may be associated with visceral larva migrans. Unilateral visual disturbances, strabismus, and eye pain are the most common presenting symptoms. The disease presents with an eosinophilic granulomatous mass, most commonly in the posterior pole of the retina. The granulmatous mass develops around the entrapped larva, in an attempt to contain the spread of the larva. ELISA testing of intraocular fluids has been demonstrated to be of great