Two-hybrid screening developed in a silkworm cell line from the larva or caterpillar of the domesticated silk moth, "Bombyx mori" (BmN4 cells). This system uses the GAL4 BD and the activation domain of mouse NF-κB P65. Both are under the control of the OpIE2 promoter. By changing specific amino acids by mutating the corresponding DNA base-pairs in the plasmids used, the importance of those amino acid residues in maintaining the interaction can be determined. After using bacterial cell-based method to select DNA-binding proteins, it is necessary to check the specificity of these domains as there is a limit to the extent to
Mori Chack Mori Chack Mori Chack (森チャック "Mori Chakku", born on March 23, 1973) is the artist name of a Japanese graphic designer, who was born in Sakai, Osaka, Japan. He is famous for his Chax product line, and especially the character Gloomy Bear, a 2 m tall, violent, pink bear that eats humans. The design is an antithesis to the excessively cute products produced by Disney, Sanrio's Hello Kitty, and other companies. Mori Chack believes that humans and animals are incompatible, and an animal is wild by nature. This is expressed most pronouncedly by the "Gloomy Bear", which is often shown