Two-hybrid screening developed in a silkworm cell line from the larva or caterpillar of the domesticated silk moth, "Bombyx mori" (BmN4 cells). This system uses the GAL4 BD and the activation domain of mouse NF-κB P65. Both are under the control of the OpIE2 promoter. By changing specific amino acids by mutating the corresponding DNA base-pairs in the plasmids used, the importance of those amino acid residues in maintaining the interaction can be determined. After using bacterial cell-based method to select DNA-binding proteins, it is necessary to check the specificity of these domains as there is a limit to the extent to
Bombyx mandarina directions in a domesticated environment. Consequently, the two silkmoths have been united as subspecies of a single species; in this case the name "Bombyx mori" which was published first applies for both. However, today it is usually recognized that the domesticated silkmoth is entirely dependent on human care for its survival and thus has a level of reproductive isolation from its wild relatives. Bombyx mandarina Bombyx mandarina, the wild silkmoth, is an insect from the moth family Bombycidae. It is the closest relative of "Bombyx mori" the domesticated silkmoth or "silkworm" (properly, this refers to the caterpillars only). Unlike the