Two-hybrid screening developed in a silkworm cell line from the larva or caterpillar of the domesticated silk moth, "Bombyx mori" (BmN4 cells). This system uses the GAL4 BD and the activation domain of mouse NF-κB P65. Both are under the control of the OpIE2 promoter. By changing specific amino acids by mutating the corresponding DNA base-pairs in the plasmids used, the importance of those amino acid residues in maintaining the interaction can be determined. After using bacterial cell-based method to select DNA-binding proteins, it is necessary to check the specificity of these domains as there is a limit to the extent to
Mori Ōgai case. Mori Ōgai discovered the cause of the beriberi disease and managed to create a foundation to build a remedy, but the problem was only resolved after his death. He was appointed director of the Imperial Museum when he retired in 1916. Mori Ōgai then died of renal failure and pulmonary tuberculosis six years later, aged 60. Although Mori did little writing from 1892 to 1902, he continued to edit a literary journal ("Mezamashi gusa", 1892–1909). He also produced translations of the works of Goethe, Schiller, Ibsen, Hans Christian Andersen, and Hauptmann. It was during the Russo-Japanese War (1904–05) that