Two-hybrid screening developed in a silkworm cell line from the larva or caterpillar of the domesticated silk moth, "Bombyx mori" (BmN4 cells). This system uses the GAL4 BD and the activation domain of mouse NF-κB P65. Both are under the control of the OpIE2 promoter. By changing specific amino acids by mutating the corresponding DNA base-pairs in the plasmids used, the importance of those amino acid residues in maintaining the interaction can be determined. After using bacterial cell-based method to select DNA-binding proteins, it is necessary to check the specificity of these domains as there is a limit to the extent to
Mori Trust Mori Trust Mori Trust was founded in 1970 as Mori Building Development, a subsidiary of the Mori Building group founded by Taikichiro Mori. Following his death in 1993, his two sons Minoru Mori and Akira Mori disagreed as to how to continue the business, with Minoru wishing to continue the company's focus on existing properties and new large-scale urban projects, and Akira wishing to expand the company's portfolio into more small-scale office and leisure properties. This led to a split of the Mori Building group, with Akira Mori taking over the Mori Building Development entity and renaming it "Mori Trust"