Two-hybrid screening developed in a silkworm cell line from the larva or caterpillar of the domesticated silk moth, "Bombyx mori" (BmN4 cells). This system uses the GAL4 BD and the activation domain of mouse NF-κB P65. Both are under the control of the OpIE2 promoter. By changing specific amino acids by mutating the corresponding DNA base-pairs in the plasmids used, the importance of those amino acid residues in maintaining the interaction can be determined. After using bacterial cell-based method to select DNA-binding proteins, it is necessary to check the specificity of these domains as there is a limit to the extent to
Kambei Mori Kambei Mori, OCLC/WorldCat encompasses 2 works in 3 publications in 1 language and 5 library holdings. Kambei Mori Kambei Mori or , also known as Mōri Kambei Shigeyoshi , was a Japanese mathematician in the Edo period. Some 16th-century sources suggest that Mori studied in China, but such claims are inconclusive or rejected by historians. What is known with certainty is that he started a school in Kyoto and he wrote several influential and widely discussed books which dealt with arithmetic and the use of the abacus. One of his students was Yoshida Mitsuyoshi the author of "Jinkōki," which is