Two-hybrid screening developed in a silkworm cell line from the larva or caterpillar of the domesticated silk moth, "Bombyx mori" (BmN4 cells). This system uses the GAL4 BD and the activation domain of mouse NF-κB P65. Both are under the control of the OpIE2 promoter. By changing specific amino acids by mutating the corresponding DNA base-pairs in the plasmids used, the importance of those amino acid residues in maintaining the interaction can be determined. After using bacterial cell-based method to select DNA-binding proteins, it is necessary to check the specificity of these domains as there is a limit to the extent to
Maru Maru Mori Mori! Maru Maru Mori Mori! On 4 April 2011, it was announced that the theme song of the Fuji Television television drama "Marumo no Okite" will be sung by actress Mana Ashida and Fuku Suzuki, both of whom are also starring in the television drama. They formed a temporary group called Kaoru to Tomoki, Tamani Mook, and the song that they will be singing is entitled "Maru Maru Mori Mori!" This singles album debuted on the Oricon charts at the third position with sales of around 35 thousand albums in the first week. This makes the group the youngest group ever