Appaloosa occurs in several overlay patterns on one of several recognized base coat colors. There are three other distinctive, "core" characteristics: mottled skin, striped hooves, and eyes with a white sclera. Skin mottling is usually seen around the muzzle, eyes, anus, and genitalia. Striped hooves are a common trait, quite noticeable on Appaloosas, but not unique to the breed. The sclera is the part of the eye surrounding the iris; although all horses show white around the eye if the eye is rolled back, to have a readily visible white sclera with the eye in a normal position is a distinctive
Eye color therefore should protect their eyes from direct exposure to sunlight. Wilson's disease involves a mutation of the gene coding for the enzyme ATPase7B, which prevents copper within the liver from entering the Golgi apparatus in cells. Instead, the copper accumulates in the liver and in other tissues, including the iris of the eye. This results in the formation of Kayser–Fleischer rings, which are dark rings that encircle the periphery of the iris. Eye color outside of the iris may also be symptomatic of disease. Yellowing of the sclera (the "whites of the eyes") is associated with jaundice, and may be