Tilling (Sussex) Tilling Green, in the fictional county of Ledshire, appeared in one of Patricia Wentworth's Miss Silver stories, "Poison in the Pen" (1955). Tilling (Sussex) Tilling is a fictional coastal town, based on Rye, East Sussex, in the "Mapp and Lucia" novels of Edward Frederic Benson (1867–1940). Tilling takes its name from the River Tillingham which flows through Rye. Benson himself moved to Rye in 1918, where he lived in Lamb House, former home of the novelist Henry James. Benson was mayor of Rye 1934-7 and was elected Speaker of the Cinque Ports in 1936. Tilling first appeared in "Miss Mapp"
TILLING (molecular biology) market including Frontier Genomics’ SNiPerase enzymes, which have been optimized for use on platforms that use labeled and unlabeled PCR products (see next section). Transgenomic isolated the single strand nuclease protein and sells it as a recombinant form. The advantage of the recombinant form is that unlike the enzyme mixtures, it does not contain non-specific nuclease activity, which can degrade the dyes on the PCR primers. The disadvantage is a substantially higher cost. The first paper describing TILLING used HPLC to identify mutations (McCallum et al., 2000a). The method was made more high throughput by using the restriction enzyme Cel-I