In the Sweet Pie and Pie by covering him in pie from head to toe. "In the Sweet Pie and Pie" was filmed between April 30 and May 3, 1941. The film makes references to several popular songs/series' of the era: Footage was borrowed from earlier shorts and reused for "In the Sweet Pie and Pie: "In the Sweet Pie and Pie" marked the final appearance of supporting actor Richard Fiske. A perfect foil for the Stooges, Fiske's promising career was cut short when he was killed in action during World War II. Larry Fine recalled that the most grueling scenes in "In the Sweet Pie
Cap analysis gene expression Input Carrier-CAGE"). Cap analysis gene expression Cap analysis gene expression (CAGE) is a gene expression technique used in molecular biology to produce a snapshot of the 5′ end of the messenger RNA population in a biological sample (the transcriptome). The small fragments (historically 27 nucleotides long, but now limited only by sequencing technologies) from the very beginnings of mRNAs (5' ends of capped transcripts) are extracted, reverse-transcribed to DNA, PCR amplified and sequenced. CAGE was first published by Hayashizaki, Carninci and co-workers in 2003. CAGE has been extensively used within the FANTOM research projects. The output of CAGE is a