Riseholme (fictional village) Poppy, Duchess of Sheffield, at nearby Sheffield Castle (which Benson located at "Sheffield Bottom"—unconnected to the Yorkshire Sheffield). It was revealed in "Mapp and Lucia" that Lucia's great rival in Tilling, Elizabeth Mapp ("Miss Mapp", 1922) had once visited Riseholme and stayed at the Ambermere Arms. She also hijacked Lucia's "au reservoir" (for "au revoir") and presented it to Tilling society as her own. Cynthia and Tony Revell (1984) "Mr Benson remembered in Rye, and the world of Tilling" cited Sir Steven Runciman (1903–2000), a Cambridge historian who knew Benson well, and Benson's manservant Charlie Tomalin (d.1981) for the assertion
TILLING (molecular biology) market including Frontier Genomics’ SNiPerase enzymes, which have been optimized for use on platforms that use labeled and unlabeled PCR products (see next section). Transgenomic isolated the single strand nuclease protein and sells it as a recombinant form. The advantage of the recombinant form is that unlike the enzyme mixtures, it does not contain non-specific nuclease activity, which can degrade the dyes on the PCR primers. The disadvantage is a substantially higher cost. The first paper describing TILLING used HPLC to identify mutations (McCallum et al., 2000a). The method was made more high throughput by using the restriction enzyme Cel-I