Riseholme (fictional village) in "Lucia in London" (1927), in which Lucia launched herself on London society; "Mapp and Lucia" (1931), in which, following Peppino's death, both Lucia and Georgie (who entered into a companionable marriage in "Lucia's Progress", 1935), took holiday lets in the Sussex town of Tilling (based on Rye) where, at the end of the summer of 1930, they decided to settle. At Tilling Lucia unveiled her celebrated dish, Lobster "à la Riseholme". Lucia and Georgie visited Riseholme again in "Trouble for Lucia" (1939) where they stayed at the home of the operatic "prima donna" Olga Bracely and Lucia called on
TILLING (molecular biology) TILLING (molecular biology) TILLING (Targeting Induced Local Lesions in Genomes) is a method in molecular biology that allows directed identification of mutations in a specific gene. TILLING was introduced in 2000, using the model plant "Arabidopsis thaliana". TILLING has since been used as a reverse genetics method in other organisms such as zebrafish, corn, wheat, rice, soybean, tomato and lettuce. The method combines a standard and efficient technique of mutagenesis using a chemical mutagen such as ethyl methanesulfonate (EMS) with a sensitive DNA screening-technique that identifies single base mutations (also called point mutations) in a target gene. The TILLING method