Tobacco methods. Some examples are: Smoking in public was, for a long time, reserved for men, and when done by women was sometimes associated with promiscuity; in Japan, during the Edo period, prostitutes and their clients often approached one another under the guise of offering a smoke. The same was true in 19th-century Europe. Following the American Civil War, the use of tobacco, primarily in cigars, became associated with masculinity and power. Today, tobacco use is often stigmatized; this has spawned quitting associations and antismoking campaigns. Bhutan is the only country in the world where tobacco sales are illegal. Due to
Tobacco smoke form a monoprotonated species or two protons to form the diprotonated species. Nicotine in tobacco smoke exists in either a protonated or unprotonated form, depending on the presence of natural acids and bases, the tobacco blend, tip ventilation, and the use of additives. Whereas protonated nicotine is positively charged and not volatile, unprotonated nicotine ("free-base nicotine") from tobacco smoke is volatile and readily passes into lipid cell membranes. More unprotonated nicotine in a puff delivers more nicotine to the brain. The same holds true for other (tobacco and non-tobacco) alkaloids. The approximate dividing line between dominance by protonated forms or