In the Sweet Pie and Pie by covering him in pie from head to toe. "In the Sweet Pie and Pie" was filmed between April 30 and May 3, 1941. The film makes references to several popular songs/series' of the era: Footage was borrowed from earlier shorts and reused for "In the Sweet Pie and Pie: "In the Sweet Pie and Pie" marked the final appearance of supporting actor Richard Fiske. A perfect foil for the Stooges, Fiske's promising career was cut short when he was killed in action during World War II. Larry Fine recalled that the most grueling scenes in "In the Sweet Pie
Cap analysis gene expression al." to cleave tags with EcoP15I and sequence them on the Illumina-Solexa platform. In 2013, Batut "et al." combined CAP trapper, template switching, and 5′-phosphate-dependent exonuclease digestion in "RAMPAGE" to maximize promoter specificity. In 2014, Murata "et al." published the "nAnTi-CAGE" protocol, where capped 5′ ends are sequenced on the Illumina platform with no PCR amplification and no tag cleavage. In 2017, Poulain "et al." updated the "nanoCAGE" protocol to use the "tagmentation" method (based on Tn5 transposition) for multiplexing. In 2018, Cvetesic " et al." increased the sensitivity of CAP-trapped CAGE by introducing selectively degradable carrier RNA (SLIC-CAGE, "Super-Low