Riseholme (fictional village) Poppy, Duchess of Sheffield, at nearby Sheffield Castle (which Benson located at "Sheffield Bottom"—unconnected to the Yorkshire Sheffield). It was revealed in "Mapp and Lucia" that Lucia's great rival in Tilling, Elizabeth Mapp ("Miss Mapp", 1922) had once visited Riseholme and stayed at the Ambermere Arms. She also hijacked Lucia's "au reservoir" (for "au revoir") and presented it to Tilling society as her own. Cynthia and Tony Revell (1984) "Mr Benson remembered in Rye, and the world of Tilling" cited Sir Steven Runciman (1903–2000), a Cambridge historian who knew Benson well, and Benson's manservant Charlie Tomalin (d.1981) for the assertion
TILLING (molecular biology) TILLING (molecular biology) TILLING (Targeting Induced Local Lesions in Genomes) is a method in molecular biology that allows directed identification of mutations in a specific gene. TILLING was introduced in 2000, using the model plant "Arabidopsis thaliana". TILLING has since been used as a reverse genetics method in other organisms such as zebrafish, corn, wheat, rice, soybean, tomato and lettuce. The method combines a standard and efficient technique of mutagenesis using a chemical mutagen such as ethyl methanesulfonate (EMS) with a sensitive DNA screening-technique that identifies single base mutations (also called point mutations) in a target gene. The TILLING method