Tilling (Sussex) for television, McConnell was introduced to Elizabeth Mapp by her intoxicated husband, Major Mapp-Flint, as "a veritable pilling of Tillar".) The custom in Tilling of saying ""au reservoir"" as a valediction (in place of the French" au revoir") was a feature of "Miss Mapp", although it became apparent in "Mapp and Lucia" that it had originated with Lucia in Riseholme. It was transported to Tilling by Elizabeth Mapp who had stayed one summer at the Ambermere Arms in Riseholme. The lexicographer Eric Partridge suggested that in fact the term had originated in America in the 1880s. Lucia's celebrated recipe Lobster
TILLING (molecular biology) TILLING (molecular biology) TILLING (Targeting Induced Local Lesions in Genomes) is a method in molecular biology that allows directed identification of mutations in a specific gene. TILLING was introduced in 2000, using the model plant "Arabidopsis thaliana". TILLING has since been used as a reverse genetics method in other organisms such as zebrafish, corn, wheat, rice, soybean, tomato and lettuce. The method combines a standard and efficient technique of mutagenesis using a chemical mutagen such as ethyl methanesulfonate (EMS) with a sensitive DNA screening-technique that identifies single base mutations (also called point mutations) in a target gene. The TILLING method