Riseholme (fictional village) Poppy, Duchess of Sheffield, at nearby Sheffield Castle (which Benson located at "Sheffield Bottom"—unconnected to the Yorkshire Sheffield). It was revealed in "Mapp and Lucia" that Lucia's great rival in Tilling, Elizabeth Mapp ("Miss Mapp", 1922) had once visited Riseholme and stayed at the Ambermere Arms. She also hijacked Lucia's "au reservoir" (for "au revoir") and presented it to Tilling society as her own. Cynthia and Tony Revell (1984) "Mr Benson remembered in Rye, and the world of Tilling" cited Sir Steven Runciman (1903–2000), a Cambridge historian who knew Benson well, and Benson's manservant Charlie Tomalin (d.1981) for the assertion
TILLING (molecular biology) EcoTILLING which uses an inexpensive method to identify fragments. Since the advent of NGS sequencing technologies, TILLING-by-sequencing has been developed based on Illumina sequencing of target genes amplified from multidimensionally pooled templates to identify possible single-nucleotide changes. There are several sources for single strand nucleases. The first widely used enzyme was mung bean nuclease, but this nuclease has been shown to have high non-specific activity, and only works at low pH, which can degrade PCR products and dye-labeled primers. The original source for single strand nuclease was from CEL1, or CJE (celery juice extract), but other products have entered the