Tobacco methods. Some examples are: Smoking in public was, for a long time, reserved for men, and when done by women was sometimes associated with promiscuity; in Japan, during the Edo period, prostitutes and their clients often approached one another under the guise of offering a smoke. The same was true in 19th-century Europe. Following the American Civil War, the use of tobacco, primarily in cigars, became associated with masculinity and power. Today, tobacco use is often stigmatized; this has spawned quitting associations and antismoking campaigns. Bhutan is the only country in the world where tobacco sales are illegal. Due to
Animals and tobacco smoke studies have also been conducted to determine the carcinogenicity of environmental tobacco to animals. These studies typically fall under the categories of simulated environmental tobacco, administering condensates of sidestream smoke, or observational studies of cancer among pets. To study the consequences of passive smoking, scientists expose animals to sidestream smoke, that which emanates from the cigarette's burning cone and through its paper, or a combination of mainstream and sidestream smoke. The IARC monographs conclude that mice with prolonged exposure to simulated second-hand smoke, that is six hours a day, five days a week, for five months with a subsequent four-month