Tilling (Sussex) "à la Riseholme" was first served in Tilling in "Mapp and Lucia". A railway station opened in Rye in 1851 and there are various references in the Benson books to a local station. In the BBC's 2014 dramatisation, townsfolk gather at a station depicted as "Tilling Town" in the hope of catching a glimpse of the Prince of Wales. In John van Druten's play, "Make Way for Lucia" (1948), based on Benson's novels, Lucia refers to Georgie Pillson and Major Benjamin Flint having breakfasted together in East Tilling. This appears to be the next station on the line between Tilling
TILLING (molecular biology) TILLING (molecular biology) TILLING (Targeting Induced Local Lesions in Genomes) is a method in molecular biology that allows directed identification of mutations in a specific gene. TILLING was introduced in 2000, using the model plant "Arabidopsis thaliana". TILLING has since been used as a reverse genetics method in other organisms such as zebrafish, corn, wheat, rice, soybean, tomato and lettuce. The method combines a standard and efficient technique of mutagenesis using a chemical mutagen such as ethyl methanesulfonate (EMS) with a sensitive DNA screening-technique that identifies single base mutations (also called point mutations) in a target gene. The TILLING method