Tilling (Sussex) Tilling Green, in the fictional county of Ledshire, appeared in one of Patricia Wentworth's Miss Silver stories, "Poison in the Pen" (1955). Tilling (Sussex) Tilling is a fictional coastal town, based on Rye, East Sussex, in the "Mapp and Lucia" novels of Edward Frederic Benson (1867–1940). Tilling takes its name from the River Tillingham which flows through Rye. Benson himself moved to Rye in 1918, where he lived in Lamb House, former home of the novelist Henry James. Benson was mayor of Rye 1934-7 and was elected Speaker of the Cinque Ports in 1936. Tilling first appeared in "Miss Mapp"
TILLING (molecular biology) EcoTILLING which uses an inexpensive method to identify fragments. Since the advent of NGS sequencing technologies, TILLING-by-sequencing has been developed based on Illumina sequencing of target genes amplified from multidimensionally pooled templates to identify possible single-nucleotide changes. There are several sources for single strand nucleases. The first widely used enzyme was mung bean nuclease, but this nuclease has been shown to have high non-specific activity, and only works at low pH, which can degrade PCR products and dye-labeled primers. The original source for single strand nuclease was from CEL1, or CJE (celery juice extract), but other products have entered the