Tilling (Sussex) of Elizabeth Mapp and subsequently of Lucia, who renamed it Mallards House. Cynthia & Tony Reavell (1984) "E F Benson: Mr Benson remembered in Rye, and the World of Tilling" contained a map of Tilling that drew on references in the books and the layout of Rye itself. A similar plan was reproduced in Holt's novels. In some instances, the street names of Tiling and Rye coincided – for example, High Street (the location of Godiva Plaistow's house, "Wasters") and West Street ("Quaint" Irene Coles' "Taormina") – but there were some variations: Mermaid Street became, in Tilling, Porpoise Street (where
TILLING (molecular biology) relies on the formation of DNA heteroduplexes that are formed when multiple alleles are amplified by PCR and are then heated and slowly cooled. A “bubble” forms at the mismatch of the two DNA strands, which is then cleaved by a single stranded nucleases. The products are then separated by size on several different platforms (see below). Mismatches may be due to induced mutation, heterozygosity within an individual, or natural variation between individuals. EcoTILLING is a method that uses TILLING techniques to look for natural mutations in individuals, usually for population genetics analysis. DEcoTILLING is a modification of TILLING and