Riseholme (fictional village) in "Lucia in London" (1927), in which Lucia launched herself on London society; "Mapp and Lucia" (1931), in which, following Peppino's death, both Lucia and Georgie (who entered into a companionable marriage in "Lucia's Progress", 1935), took holiday lets in the Sussex town of Tilling (based on Rye) where, at the end of the summer of 1930, they decided to settle. At Tilling Lucia unveiled her celebrated dish, Lobster "à la Riseholme". Lucia and Georgie visited Riseholme again in "Trouble for Lucia" (1939) where they stayed at the home of the operatic "prima donna" Olga Bracely and Lucia called on
TILLING (molecular biology) relies on the formation of DNA heteroduplexes that are formed when multiple alleles are amplified by PCR and are then heated and slowly cooled. A “bubble” forms at the mismatch of the two DNA strands, which is then cleaved by a single stranded nucleases. The products are then separated by size on several different platforms (see below). Mismatches may be due to induced mutation, heterozygosity within an individual, or natural variation between individuals. EcoTILLING is a method that uses TILLING techniques to look for natural mutations in individuals, usually for population genetics analysis. DEcoTILLING is a modification of TILLING and