Dutch Americans to Dutch places (for example Brooklyn which stems from Breukelen) - can still be seen. There are about 35 Dutch restaurants and bakeries in the United States, most of them founded in the 20th century. New York City for example has many originally Dutch street and place names which range from Coney Island and Brooklyn to Wall Street and Broadway. And up the river in New York State Piermont, Orangeburg, Blauvelt and Haverstraw, just to name a few places. In the Hudson Valley region there are many places and waterways whose names incorporate the word "-kill", Dutch for "stream" or
Rabbit hybridoma contrast microscope. Selected subclones were further tested for their ability to produce a stable hybridoma and monoclonal antibody secretion. After multiple rounds of subcloning and selection processes, a new cell line named 240E-W, was identified and which expressed better fusion efficiency and stability. Cell line 240E-W has since been further developed and optimized for production of rabbit monoclonal antibodies for research and commercial applications. The process of hybridoma formation in a rabbit first entails obtaining B-cells from a rabbit that has been immunized. There are numerous immunization protocols for rabbit, notably for the generation of polyclonal antibodies. After immunization, B-cells