Tilling (Sussex) for television, McConnell was introduced to Elizabeth Mapp by her intoxicated husband, Major Mapp-Flint, as "a veritable pilling of Tillar".) The custom in Tilling of saying ""au reservoir"" as a valediction (in place of the French" au revoir") was a feature of "Miss Mapp", although it became apparent in "Mapp and Lucia" that it had originated with Lucia in Riseholme. It was transported to Tilling by Elizabeth Mapp who had stayed one summer at the Ambermere Arms in Riseholme. The lexicographer Eric Partridge suggested that in fact the term had originated in America in the 1880s. Lucia's celebrated recipe Lobster
TILLING (molecular biology) relies on the formation of DNA heteroduplexes that are formed when multiple alleles are amplified by PCR and are then heated and slowly cooled. A “bubble” forms at the mismatch of the two DNA strands, which is then cleaved by a single stranded nucleases. The products are then separated by size on several different platforms (see below). Mismatches may be due to induced mutation, heterozygosity within an individual, or natural variation between individuals. EcoTILLING is a method that uses TILLING techniques to look for natural mutations in individuals, usually for population genetics analysis. DEcoTILLING is a modification of TILLING and