Animal echolocation in the external ear. There are two hypotheses about the evolution of echolocation in bats. The first suggests that laryngeal echolocation evolved twice in Chiroptera, once in the Yangochiroptera and once in the horseshoe bats (Rhinolophidae). The second proposes that laryngeal echolocation had a single origin in Chiroptera, was subsequently lost in the family Pteropodidae (all megabats), and later evolved as a system of tongue-clicking in the genus "Rousettus". Individual bat species echolocate within specific frequency ranges that suit their environment and prey types. This has sometimes been used by researchers to identify bats flying in an area simply by
Virginia big-eared bat make sounds that then echo off of objects around them, enabling them to find their prey while flying in the dark. Typically they prey on insects like moths, beetles, termites, mosquitoes and flies. Without echolocation, the bats would not be able to locate food. When analyzed, 97% of Virginia big-eared bat fecal pellets showed that the bat had consumed and digested Lepidoptera. Lepidoptera is a classification of insects that contains butterflies and moths. In Kentucky a similar study was done, examining the fecal pellets and stomach contents of the bats in and around the caves. The processed food showed that