Tilling (Sussex) and the spring of 1931, in which Emmeline Lucas ("Lucia") and Elizabeth Mapp clashed for the first time, "Lucia's Progress" (1935) and "Trouble for Lucia" (1939). The novelist Susan Leg, the subject of "Secret Lives" (1932), re-appeared in "Trouble for Lucia". The first Lucia book, "Queen Lucia", was published in 1920. It was followed in 1927 by "Lucia in London". Benson's "The Freaks of Mayfair" (1916) provided the genesis of some of the characters of Tilling. Specifically, "Aunt Georgie", a bachelor with a penchant for embroidery, provided the model for George Pillson, who, as with Lucia, with whom he entered
TILLING (molecular biology) market including Frontier Genomics’ SNiPerase enzymes, which have been optimized for use on platforms that use labeled and unlabeled PCR products (see next section). Transgenomic isolated the single strand nuclease protein and sells it as a recombinant form. The advantage of the recombinant form is that unlike the enzyme mixtures, it does not contain non-specific nuclease activity, which can degrade the dyes on the PCR primers. The disadvantage is a substantially higher cost. The first paper describing TILLING used HPLC to identify mutations (McCallum et al., 2000a). The method was made more high throughput by using the restriction enzyme Cel-I