Machine perfusion immediate contralateral nephrectomy. Their best dog had a peak post reimplantation creatinine of 50 mg/l (0.44 mmol/l). Liu used well hydrated dogs undergoing a mannitol diuresis and stored the kidneys at 9 °C – 10 °C using a perfusate derived from human PPF. The PPF was further fractionated by using a highly water-soluble polymer (Pluronic F-38), and sodium acetyl tryptophanate and sodium caprylate were added to the PPF as stabilisers to permit pasteurisation. To this solution were added human albumin, heparin, mannitol, glucose, magnesium sulphate, potassium chloride, insulin, methyl prednisolone, carbenicillin, and water to adjust the osmolality to 300-310 mosmol/kg.
U Should've Known Better song entered the "Billboard" Hot 100 chart, where it debuted at number 67 in the week of June 6, 2004, the second-highest debut of the week. "U Should've Known Better" remained twenty weeks on the chart, reaching its peak position of number 19 in its ninth week. It marked the second single from "After the Storm" to reach the top twenty on the Hot 100chart and, as the album's final single, would remain its second highest-charting offering behind leading single "So Gone." Although never released on a CD single or CD maxi single format, "U Should've Known Better" was also