Machine perfusion immediate contralateral nephrectomy. Their best dog had a peak post reimplantation creatinine of 50 mg/l (0.44 mmol/l). Liu used well hydrated dogs undergoing a mannitol diuresis and stored the kidneys at 9 °C – 10 °C using a perfusate derived from human PPF. The PPF was further fractionated by using a highly water-soluble polymer (Pluronic F-38), and sodium acetyl tryptophanate and sodium caprylate were added to the PPF as stabilisers to permit pasteurisation. To this solution were added human albumin, heparin, mannitol, glucose, magnesium sulphate, potassium chloride, insulin, methyl prednisolone, carbenicillin, and water to adjust the osmolality to 300-310 mosmol/kg.
U Should've Known Better producers, which included Dallas Austin, production team Soulshock & Karlin, Bryan Michael Cox, and Rodney Jerkins and his Darkchild crew. Though she "had never thought about writing much" by then, her producers encouraged the singer to intensify her work on the "All Eyez on Me" album and to write and contribute own lyrics and ideas to the songs, one of which was the ballad "U Should've Known Better." Monica penned the song along with Harold Lilly and longtime contributor Jermaine Dupri, while production on the track was helmed by Dupri and Bryan Michael Cox. "U Should've Known Better" was mixed