Dhansak consumed for three days after the death of a near one. And dhansak is used to break this abstinence on the fourth day. Hence, dhansak is never prepared on auspicious occasions like festivals and weddings. Dhansak is made by cooking mutton cubes with a mixture of various lentils and vegetables. Traditionally, four lentils (arhar dal, Bengal gram or chana dal, red masoor dal and brown masoor dal) are used, but one or more of the lentils may be omitted or substituted. The vegetables include potato, tomato, brinjal, pumpkin and fenugreek leaves: again, substitutions, such as squash for pumpkin, and sweet
Pulse-chase analysis the pulse period could still be tracked within the cell. This method is useful for determining the activity of certain cells over a prolonged period of time. The method has been used to study protein kinase C, ubiquitin, and many other proteins. The method was also used to prove the existence and function of Okazaki fragments. George Palade used pulse-chase of radioactive amino acids to elucidate the secretory pathway. Pulse-chase analysis In biochemistry and molecular biology, a pulse-chase analysis is a method for examining a cellular process occurring over time by successively exposing the cells to a labeled compound (pulse)