History of New York City (prehistory–1664) with the ethnic and racial balance of the community. The economic activity brought in a wide variety of ethnic groups to the fledgling city during the 17th century, including Spanish, Jews, and Africans, some of them as slaves. The Dutch origins can still be seen in many names in New York City, such as Coney Island (from "Konijnen Eiland" – Dutch for "Rabbit Island"), Bowery from "bouwerij" (modern Dutch "boerderij" = "farm"), Brooklyn (from Breukelen), Harlem from Haarlem (formalized in 1658 as "Nieuw Haarlem"), Greenwich Village (from Greenwijck, meaning "pine wood quarter"), Flushing (from Vlissingen) and Staten Island (from "Staaten
Rabbit hybridoma contrast microscope. Selected subclones were further tested for their ability to produce a stable hybridoma and monoclonal antibody secretion. After multiple rounds of subcloning and selection processes, a new cell line named 240E-W, was identified and which expressed better fusion efficiency and stability. Cell line 240E-W has since been further developed and optimized for production of rabbit monoclonal antibodies for research and commercial applications. The process of hybridoma formation in a rabbit first entails obtaining B-cells from a rabbit that has been immunized. There are numerous immunization protocols for rabbit, notably for the generation of polyclonal antibodies. After immunization, B-cells