Tilling (Sussex) Tilling Green, in the fictional county of Ledshire, appeared in one of Patricia Wentworth's Miss Silver stories, "Poison in the Pen" (1955). Tilling (Sussex) Tilling is a fictional coastal town, based on Rye, East Sussex, in the "Mapp and Lucia" novels of Edward Frederic Benson (1867–1940). Tilling takes its name from the River Tillingham which flows through Rye. Benson himself moved to Rye in 1918, where he lived in Lamb House, former home of the novelist Henry James. Benson was mayor of Rye 1934-7 and was elected Speaker of the Cinque Ports in 1936. Tilling first appeared in "Miss Mapp"
TILLING (molecular biology) relies on the formation of DNA heteroduplexes that are formed when multiple alleles are amplified by PCR and are then heated and slowly cooled. A “bubble” forms at the mismatch of the two DNA strands, which is then cleaved by a single stranded nucleases. The products are then separated by size on several different platforms (see below). Mismatches may be due to induced mutation, heterozygosity within an individual, or natural variation between individuals. EcoTILLING is a method that uses TILLING techniques to look for natural mutations in individuals, usually for population genetics analysis. DEcoTILLING is a modification of TILLING and