Megabat evolved early in the lineage of chiropterans. Although echolocation was later lost in family Pteropodidae, bats in the genus "Rousettus" are capable of primitive echolocation through clicking their tongues, and some species have been shown to create clicks similar to those of echolocating bats using their wings. Both echolocation and flight are energetically expensive processes. The nature of the flight and echolocation mechanism of bats allows for creation of echolocation pulses with minimal energy use. Energetic coupling of these two processes is thought to have allowed for both energetically expensive processes to evolve in bats. The loss of echolocation may
Lazzaro Spallanzani with frogs, and an artificial insemination, using a dog. Spallanzani showed that some animals, especially newts, can regenerate some parts of their body if injured or surgically removed. Spallanzani is also credited with the classification of tardigrades, which are one of the most durable extremophiles still to this day. (See Binomial Nomenclature) Spallanzani is also famous for extensive experiments on the navigation in complete darkness by bats, where he concluded that bats do not use their eyes for navigation in total darkness, but some other sense (see animal echolocation). He was the pioneer of the original study of echolocation, though