Pollen DNA barcoding networks which are made up of all the interactions between plants and the animals that facilitate their pollination. Identifying the pollen carried on insects helps scientists understand what plants are being visited by which insects. Insects can also have homologous features making them difficult to identify and are themselves sometimes identified through genetic barcoding (usually of the CO1 region). Every insect that visits a flower isn’t necessarily a pollinator. Many lack features such as hairs allowing them to carry pollen while others avoid the pollen-laden anthers to steal nectar. Pollination networks are made more accurate by including what pollen is
Pollen DNA barcoding that has grown in popularity due to the decreased costs associated with "next generation sequencing" (NGS) techniques and is being continually improved in efficiency including through the use of a dual-indexing approach. Some of the other major advantages include the savings in time and resources compared to microscopic identification. Identifying pollen is time consuming involving spreading pollen on a slide, staining the pollen to improve visibility, then focusing in on individual pollen grains and identifying them based on size, shape, as well as the shape and number of pores. If a pollen reference library is not available, then pollen has