Tilling (Sussex) for television, McConnell was introduced to Elizabeth Mapp by her intoxicated husband, Major Mapp-Flint, as "a veritable pilling of Tillar".) The custom in Tilling of saying ""au reservoir"" as a valediction (in place of the French" au revoir") was a feature of "Miss Mapp", although it became apparent in "Mapp and Lucia" that it had originated with Lucia in Riseholme. It was transported to Tilling by Elizabeth Mapp who had stayed one summer at the Ambermere Arms in Riseholme. The lexicographer Eric Partridge suggested that in fact the term had originated in America in the 1880s. Lucia's celebrated recipe Lobster
TILLING (molecular biology) market including Frontier Genomics’ SNiPerase enzymes, which have been optimized for use on platforms that use labeled and unlabeled PCR products (see next section). Transgenomic isolated the single strand nuclease protein and sells it as a recombinant form. The advantage of the recombinant form is that unlike the enzyme mixtures, it does not contain non-specific nuclease activity, which can degrade the dyes on the PCR primers. The disadvantage is a substantially higher cost. The first paper describing TILLING used HPLC to identify mutations (McCallum et al., 2000a). The method was made more high throughput by using the restriction enzyme Cel-I