In the Sweet Pie and Pie by covering him in pie from head to toe. "In the Sweet Pie and Pie" was filmed between April 30 and May 3, 1941. The film makes references to several popular songs/series' of the era: Footage was borrowed from earlier shorts and reused for "In the Sweet Pie and Pie: "In the Sweet Pie and Pie" marked the final appearance of supporting actor Richard Fiske. A perfect foil for the Stooges, Fiske's promising career was cut short when he was killed in action during World War II. Larry Fine recalled that the most grueling scenes in "In the Sweet Pie
Cap analysis gene expression gene expression. However, the CAGE protocol has a known bias with a nonspecific guanine (G) at the most 5′ end of the CAGE tags, which is attributed to the template-free 5′-extension during the first-strand cDNA synthesis. This would induce erroneous mapping of CAGE tags, for instance to nontranscribed pseudogenes. On the other hand, this addition of Gs was also utilised as a signal to filter more reliable TSS peaks. The original CAGE method (Shiraki "et al.", 2003) was using CAP Trapper for capturing the 5′ ends, oligo-dT primers for synthesizing the cDNAs, the type IIs restriction enzyme MmeI for cleaving