Riseholme (fictional village) in "Lucia in London" (1927), in which Lucia launched herself on London society; "Mapp and Lucia" (1931), in which, following Peppino's death, both Lucia and Georgie (who entered into a companionable marriage in "Lucia's Progress", 1935), took holiday lets in the Sussex town of Tilling (based on Rye) where, at the end of the summer of 1930, they decided to settle. At Tilling Lucia unveiled her celebrated dish, Lobster "à la Riseholme". Lucia and Georgie visited Riseholme again in "Trouble for Lucia" (1939) where they stayed at the home of the operatic "prima donna" Olga Bracely and Lucia called on
TILLING (molecular biology) EcoTILLING which uses an inexpensive method to identify fragments. Since the advent of NGS sequencing technologies, TILLING-by-sequencing has been developed based on Illumina sequencing of target genes amplified from multidimensionally pooled templates to identify possible single-nucleotide changes. There are several sources for single strand nucleases. The first widely used enzyme was mung bean nuclease, but this nuclease has been shown to have high non-specific activity, and only works at low pH, which can degrade PCR products and dye-labeled primers. The original source for single strand nuclease was from CEL1, or CJE (celery juice extract), but other products have entered the