Tilling (Sussex) and the spring of 1931, in which Emmeline Lucas ("Lucia") and Elizabeth Mapp clashed for the first time, "Lucia's Progress" (1935) and "Trouble for Lucia" (1939). The novelist Susan Leg, the subject of "Secret Lives" (1932), re-appeared in "Trouble for Lucia". The first Lucia book, "Queen Lucia", was published in 1920. It was followed in 1927 by "Lucia in London". Benson's "The Freaks of Mayfair" (1916) provided the genesis of some of the characters of Tilling. Specifically, "Aunt Georgie", a bachelor with a penchant for embroidery, provided the model for George Pillson, who, as with Lucia, with whom he entered
TILLING (molecular biology) EcoTILLING which uses an inexpensive method to identify fragments. Since the advent of NGS sequencing technologies, TILLING-by-sequencing has been developed based on Illumina sequencing of target genes amplified from multidimensionally pooled templates to identify possible single-nucleotide changes. There are several sources for single strand nucleases. The first widely used enzyme was mung bean nuclease, but this nuclease has been shown to have high non-specific activity, and only works at low pH, which can degrade PCR products and dye-labeled primers. The original source for single strand nuclease was from CEL1, or CJE (celery juice extract), but other products have entered the