Tilling (Sussex) Tilling Green, in the fictional county of Ledshire, appeared in one of Patricia Wentworth's Miss Silver stories, "Poison in the Pen" (1955). Tilling (Sussex) Tilling is a fictional coastal town, based on Rye, East Sussex, in the "Mapp and Lucia" novels of Edward Frederic Benson (1867–1940). Tilling takes its name from the River Tillingham which flows through Rye. Benson himself moved to Rye in 1918, where he lived in Lamb House, former home of the novelist Henry James. Benson was mayor of Rye 1934-7 and was elected Speaker of the Cinque Ports in 1936. Tilling first appeared in "Miss Mapp"
TILLING (molecular biology) TILLING (molecular biology) TILLING (Targeting Induced Local Lesions in Genomes) is a method in molecular biology that allows directed identification of mutations in a specific gene. TILLING was introduced in 2000, using the model plant "Arabidopsis thaliana". TILLING has since been used as a reverse genetics method in other organisms such as zebrafish, corn, wheat, rice, soybean, tomato and lettuce. The method combines a standard and efficient technique of mutagenesis using a chemical mutagen such as ethyl methanesulfonate (EMS) with a sensitive DNA screening-technique that identifies single base mutations (also called point mutations) in a target gene. The TILLING method