Riseholme (fictional village) Poppy, Duchess of Sheffield, at nearby Sheffield Castle (which Benson located at "Sheffield Bottom"—unconnected to the Yorkshire Sheffield). It was revealed in "Mapp and Lucia" that Lucia's great rival in Tilling, Elizabeth Mapp ("Miss Mapp", 1922) had once visited Riseholme and stayed at the Ambermere Arms. She also hijacked Lucia's "au reservoir" (for "au revoir") and presented it to Tilling society as her own. Cynthia and Tony Revell (1984) "Mr Benson remembered in Rye, and the world of Tilling" cited Sir Steven Runciman (1903–2000), a Cambridge historian who knew Benson well, and Benson's manservant Charlie Tomalin (d.1981) for the assertion
TILLING (molecular biology) relies on the formation of DNA heteroduplexes that are formed when multiple alleles are amplified by PCR and are then heated and slowly cooled. A “bubble” forms at the mismatch of the two DNA strands, which is then cleaved by a single stranded nucleases. The products are then separated by size on several different platforms (see below). Mismatches may be due to induced mutation, heterozygosity within an individual, or natural variation between individuals. EcoTILLING is a method that uses TILLING techniques to look for natural mutations in individuals, usually for population genetics analysis. DEcoTILLING is a modification of TILLING and