Tilling (Sussex) and the spring of 1931, in which Emmeline Lucas ("Lucia") and Elizabeth Mapp clashed for the first time, "Lucia's Progress" (1935) and "Trouble for Lucia" (1939). The novelist Susan Leg, the subject of "Secret Lives" (1932), re-appeared in "Trouble for Lucia". The first Lucia book, "Queen Lucia", was published in 1920. It was followed in 1927 by "Lucia in London". Benson's "The Freaks of Mayfair" (1916) provided the genesis of some of the characters of Tilling. Specifically, "Aunt Georgie", a bachelor with a penchant for embroidery, provided the model for George Pillson, who, as with Lucia, with whom he entered
TILLING (molecular biology) TILLING (molecular biology) TILLING (Targeting Induced Local Lesions in Genomes) is a method in molecular biology that allows directed identification of mutations in a specific gene. TILLING was introduced in 2000, using the model plant "Arabidopsis thaliana". TILLING has since been used as a reverse genetics method in other organisms such as zebrafish, corn, wheat, rice, soybean, tomato and lettuce. The method combines a standard and efficient technique of mutagenesis using a chemical mutagen such as ethyl methanesulfonate (EMS) with a sensitive DNA screening-technique that identifies single base mutations (also called point mutations) in a target gene. The TILLING method