Tilling (Sussex) "à la Riseholme" was first served in Tilling in "Mapp and Lucia". A railway station opened in Rye in 1851 and there are various references in the Benson books to a local station. In the BBC's 2014 dramatisation, townsfolk gather at a station depicted as "Tilling Town" in the hope of catching a glimpse of the Prince of Wales. In John van Druten's play, "Make Way for Lucia" (1948), based on Benson's novels, Lucia refers to Georgie Pillson and Major Benjamin Flint having breakfasted together in East Tilling. This appears to be the next station on the line between Tilling
TILLING (molecular biology) EcoTILLING which uses an inexpensive method to identify fragments. Since the advent of NGS sequencing technologies, TILLING-by-sequencing has been developed based on Illumina sequencing of target genes amplified from multidimensionally pooled templates to identify possible single-nucleotide changes. There are several sources for single strand nucleases. The first widely used enzyme was mung bean nuclease, but this nuclease has been shown to have high non-specific activity, and only works at low pH, which can degrade PCR products and dye-labeled primers. The original source for single strand nuclease was from CEL1, or CJE (celery juice extract), but other products have entered the