Tilling (Sussex) "à la Riseholme" was first served in Tilling in "Mapp and Lucia". A railway station opened in Rye in 1851 and there are various references in the Benson books to a local station. In the BBC's 2014 dramatisation, townsfolk gather at a station depicted as "Tilling Town" in the hope of catching a glimpse of the Prince of Wales. In John van Druten's play, "Make Way for Lucia" (1948), based on Benson's novels, Lucia refers to Georgie Pillson and Major Benjamin Flint having breakfasted together in East Tilling. This appears to be the next station on the line between Tilling
TILLING (molecular biology) market including Frontier Genomics’ SNiPerase enzymes, which have been optimized for use on platforms that use labeled and unlabeled PCR products (see next section). Transgenomic isolated the single strand nuclease protein and sells it as a recombinant form. The advantage of the recombinant form is that unlike the enzyme mixtures, it does not contain non-specific nuclease activity, which can degrade the dyes on the PCR primers. The disadvantage is a substantially higher cost. The first paper describing TILLING used HPLC to identify mutations (McCallum et al., 2000a). The method was made more high throughput by using the restriction enzyme Cel-I