Tilling (Sussex) of Elizabeth Mapp and subsequently of Lucia, who renamed it Mallards House. Cynthia & Tony Reavell (1984) "E F Benson: Mr Benson remembered in Rye, and the World of Tilling" contained a map of Tilling that drew on references in the books and the layout of Rye itself. A similar plan was reproduced in Holt's novels. In some instances, the street names of Tiling and Rye coincided – for example, High Street (the location of Godiva Plaistow's house, "Wasters") and West Street ("Quaint" Irene Coles' "Taormina") – but there were some variations: Mermaid Street became, in Tilling, Porpoise Street (where
TILLING (molecular biology) EcoTILLING which uses an inexpensive method to identify fragments. Since the advent of NGS sequencing technologies, TILLING-by-sequencing has been developed based on Illumina sequencing of target genes amplified from multidimensionally pooled templates to identify possible single-nucleotide changes. There are several sources for single strand nucleases. The first widely used enzyme was mung bean nuclease, but this nuclease has been shown to have high non-specific activity, and only works at low pH, which can degrade PCR products and dye-labeled primers. The original source for single strand nuclease was from CEL1, or CJE (celery juice extract), but other products have entered the