Riseholme (fictional village) Poppy, Duchess of Sheffield, at nearby Sheffield Castle (which Benson located at "Sheffield Bottom"—unconnected to the Yorkshire Sheffield). It was revealed in "Mapp and Lucia" that Lucia's great rival in Tilling, Elizabeth Mapp ("Miss Mapp", 1922) had once visited Riseholme and stayed at the Ambermere Arms. She also hijacked Lucia's "au reservoir" (for "au revoir") and presented it to Tilling society as her own. Cynthia and Tony Revell (1984) "Mr Benson remembered in Rye, and the world of Tilling" cited Sir Steven Runciman (1903–2000), a Cambridge historian who knew Benson well, and Benson's manservant Charlie Tomalin (d.1981) for the assertion
TILLING (molecular biology) compared to polyacrylamide systems. Elchrom Scientific sells Spreadex gels which are precast, can be high throughput and are more sensitive than standard polyacrylamide gels. Advanced Analytical Technologies Inc sells the AdvanCE FS96 dsDNA Fluorescent System which is a 96 capillary electrophoresis system that has several advantages over traditional methods; including ability to separate large fragments (up to 40kb), no desalting or precipitation step required, short run times (~30 minutes), sensitivity to 5pg/ul and no need for fluorescent labeled primers. Several TILLING centers exist over the world that focus on agriculturally important species: TILLING (molecular biology) TILLING (Targeting Induced Local Lesions