Dutch Empire and the Netherlands; Albany Dutch spoken but in danger of extinction in the USA. Extinct Dutch-based creole languages include: Skepi (Guyana); Negerhollands (aka "Negro Dutch"), Jersey Dutch and Mohawk Dutch (USA) and Javindo (Java). "See also: List of Place Names of Dutch Origin" Some towns of New York and areas of New York City, once part of the colony of New Netherland have names of Dutch origin, such as Brooklyn (after Breukelen), Flushing (after Vlissingen), the Bowery (after Bouwerij, construction site), Harlem (after Haarlem), Coney Island (from Conyne Eylandt, modern Dutch spelling Konijneneiland: Rabbit island) and Staten Island (meaning "Island
Rabbit hybridoma contrast microscope. Selected subclones were further tested for their ability to produce a stable hybridoma and monoclonal antibody secretion. After multiple rounds of subcloning and selection processes, a new cell line named 240E-W, was identified and which expressed better fusion efficiency and stability. Cell line 240E-W has since been further developed and optimized for production of rabbit monoclonal antibodies for research and commercial applications. The process of hybridoma formation in a rabbit first entails obtaining B-cells from a rabbit that has been immunized. There are numerous immunization protocols for rabbit, notably for the generation of polyclonal antibodies. After immunization, B-cells