Tilling (Sussex) of Elizabeth Mapp and subsequently of Lucia, who renamed it Mallards House. Cynthia & Tony Reavell (1984) "E F Benson: Mr Benson remembered in Rye, and the World of Tilling" contained a map of Tilling that drew on references in the books and the layout of Rye itself. A similar plan was reproduced in Holt's novels. In some instances, the street names of Tiling and Rye coincided – for example, High Street (the location of Godiva Plaistow's house, "Wasters") and West Street ("Quaint" Irene Coles' "Taormina") – but there were some variations: Mermaid Street became, in Tilling, Porpoise Street (where
TILLING (molecular biology) market including Frontier Genomics’ SNiPerase enzymes, which have been optimized for use on platforms that use labeled and unlabeled PCR products (see next section). Transgenomic isolated the single strand nuclease protein and sells it as a recombinant form. The advantage of the recombinant form is that unlike the enzyme mixtures, it does not contain non-specific nuclease activity, which can degrade the dyes on the PCR primers. The disadvantage is a substantially higher cost. The first paper describing TILLING used HPLC to identify mutations (McCallum et al., 2000a). The method was made more high throughput by using the restriction enzyme Cel-I