Riseholme (fictional village) in "Lucia in London" (1927), in which Lucia launched herself on London society; "Mapp and Lucia" (1931), in which, following Peppino's death, both Lucia and Georgie (who entered into a companionable marriage in "Lucia's Progress", 1935), took holiday lets in the Sussex town of Tilling (based on Rye) where, at the end of the summer of 1930, they decided to settle. At Tilling Lucia unveiled her celebrated dish, Lobster "à la Riseholme". Lucia and Georgie visited Riseholme again in "Trouble for Lucia" (1939) where they stayed at the home of the operatic "prima donna" Olga Bracely and Lucia called on
TILLING (molecular biology) market including Frontier Genomics’ SNiPerase enzymes, which have been optimized for use on platforms that use labeled and unlabeled PCR products (see next section). Transgenomic isolated the single strand nuclease protein and sells it as a recombinant form. The advantage of the recombinant form is that unlike the enzyme mixtures, it does not contain non-specific nuclease activity, which can degrade the dyes on the PCR primers. The disadvantage is a substantially higher cost. The first paper describing TILLING used HPLC to identify mutations (McCallum et al., 2000a). The method was made more high throughput by using the restriction enzyme Cel-I