Tilling (Sussex) and the spring of 1931, in which Emmeline Lucas ("Lucia") and Elizabeth Mapp clashed for the first time, "Lucia's Progress" (1935) and "Trouble for Lucia" (1939). The novelist Susan Leg, the subject of "Secret Lives" (1932), re-appeared in "Trouble for Lucia". The first Lucia book, "Queen Lucia", was published in 1920. It was followed in 1927 by "Lucia in London". Benson's "The Freaks of Mayfair" (1916) provided the genesis of some of the characters of Tilling. Specifically, "Aunt Georgie", a bachelor with a penchant for embroidery, provided the model for George Pillson, who, as with Lucia, with whom he entered
TILLING (molecular biology) relies on the formation of DNA heteroduplexes that are formed when multiple alleles are amplified by PCR and are then heated and slowly cooled. A “bubble” forms at the mismatch of the two DNA strands, which is then cleaved by a single stranded nucleases. The products are then separated by size on several different platforms (see below). Mismatches may be due to induced mutation, heterozygosity within an individual, or natural variation between individuals. EcoTILLING is a method that uses TILLING techniques to look for natural mutations in individuals, usually for population genetics analysis. DEcoTILLING is a modification of TILLING and