Tilling (Sussex) for television, McConnell was introduced to Elizabeth Mapp by her intoxicated husband, Major Mapp-Flint, as "a veritable pilling of Tillar".) The custom in Tilling of saying ""au reservoir"" as a valediction (in place of the French" au revoir") was a feature of "Miss Mapp", although it became apparent in "Mapp and Lucia" that it had originated with Lucia in Riseholme. It was transported to Tilling by Elizabeth Mapp who had stayed one summer at the Ambermere Arms in Riseholme. The lexicographer Eric Partridge suggested that in fact the term had originated in America in the 1880s. Lucia's celebrated recipe Lobster
TILLING (molecular biology) EcoTILLING which uses an inexpensive method to identify fragments. Since the advent of NGS sequencing technologies, TILLING-by-sequencing has been developed based on Illumina sequencing of target genes amplified from multidimensionally pooled templates to identify possible single-nucleotide changes. There are several sources for single strand nucleases. The first widely used enzyme was mung bean nuclease, but this nuclease has been shown to have high non-specific activity, and only works at low pH, which can degrade PCR products and dye-labeled primers. The original source for single strand nuclease was from CEL1, or CJE (celery juice extract), but other products have entered the