Tilling (Sussex) "à la Riseholme" was first served in Tilling in "Mapp and Lucia". A railway station opened in Rye in 1851 and there are various references in the Benson books to a local station. In the BBC's 2014 dramatisation, townsfolk gather at a station depicted as "Tilling Town" in the hope of catching a glimpse of the Prince of Wales. In John van Druten's play, "Make Way for Lucia" (1948), based on Benson's novels, Lucia refers to Georgie Pillson and Major Benjamin Flint having breakfasted together in East Tilling. This appears to be the next station on the line between Tilling
TILLING (molecular biology) relies on the formation of DNA heteroduplexes that are formed when multiple alleles are amplified by PCR and are then heated and slowly cooled. A “bubble” forms at the mismatch of the two DNA strands, which is then cleaved by a single stranded nucleases. The products are then separated by size on several different platforms (see below). Mismatches may be due to induced mutation, heterozygosity within an individual, or natural variation between individuals. EcoTILLING is a method that uses TILLING techniques to look for natural mutations in individuals, usually for population genetics analysis. DEcoTILLING is a modification of TILLING and