Nicolaes Tulp depicted in the painting can be dated to 16 January 1632; the Amsterdam Guild of Surgeons, of which Tulp was official City Anatomist, permitted only one public dissection a year and the body would have to be that of an executed criminal. The criminal is identified as the robber Aris Kindt. Rembrandt would later make a painting of Tulp's successor in 1656 "The Anatomy Lesson of Dr Jan Deijman". Since the painting of Tulp's predecessor in 1619, "The Osteology Lesson of Dr Sebastiaen Egbertsz" was a group portrait around a skeleton, it is clear that the subject of a dead
Nicolaes Tulp of his books, Tulp became Mayor of Amsterdam in 1654, a position he held for four terms. His son Dirck married Anna Burgh, the daughter of Albert Burgh, another Mayor of Amsterdam who had, like Tulp, studied medicine in Leiden in 1614. In 1655 Tulp's daughter Margaretha married Jan Six, whom he helped become a Magistrate of family affairs in Amsterdam. Years later, Six would also become Mayor of Amsterdam. Tulp, impressed by his behaviour, invited Paulus Potter to come to Amsterdam, after a quarrel in the Hague. In 1673 Tulp was admitted to the Governing Committee of the Republic