Nicolaes Tulp depicted in the painting can be dated to 16 January 1632; the Amsterdam Guild of Surgeons, of which Tulp was official City Anatomist, permitted only one public dissection a year and the body would have to be that of an executed criminal. The criminal is identified as the robber Aris Kindt. Rembrandt would later make a painting of Tulp's successor in 1656 "The Anatomy Lesson of Dr Jan Deijman". Since the painting of Tulp's predecessor in 1619, "The Osteology Lesson of Dr Sebastiaen Egbertsz" was a group portrait around a skeleton, it is clear that the subject of a dead
The Anatomy Lesson of Dr. Nicolaes Tulp referenced the painting at the bottom of page 10, where the characters observe the disembowelment of a fish. The 2011 video game "" references the painting in both in-game portraits that can be found on the wall and in a certain cinematic trailer, featuring the main protagonist Adam Jensen as the cadaver as Dr. Nicolaes and his students study his charred and ruined arms, which in the actual story become amputated and replaced with mechanical limbs. The painting is discussed by the narrator (a young man) and his mother during a visit to the Met in "The Goldfinch", a 2013