Portrait painting benefitted greatly from such commissions and from the general appreciation of art by bourgeois clients, who supported portraiture as well as still-life and landscapes painting. In addition, the first significant art and dealer markets flourished in Holland at that time. With plenty of demand, Rembrandt was able to experiment with unconventional composition and technique, such as chiaroscuro. He demonstrated these innovations, pioneered by Italian masters such as Caravaggio, most notably in his famous "Night Watch" (1642). "The Anatomy Lesson of Dr. Tulp" (1632) is another fine example of Rembrandt's mastery of the group painting, in which he bathes the corpse
Nicolaes Tulp Surgeons. His wife died in the same year, leaving him with five young children. In 1630 he married his second wife, the daughter of the mayor of Outshoorn and she bore him three children. It was Dr Tulp who examined and signed the fitness reports for the first Dutch settlers on the island of Manhattan, and his signature was found on these in the long-lost archives of the Dutch settlement uncovered in the 1980s in the basement of the New York public library. In his job, Tulp was responsible for inspections of apothecary shops. Chemists in Amsterdam had access to