Kinetic Isotope Effects of RuBisCO carbon, oxygen, and/or hydrogen, though currently only a significant isotope effect is seen for carbon isotope substitution. Isotopes are atoms that have the same number of protons but varying numbers of neutrons. "Lighter" isotopes (like the stable Carbon-12 isotope) have a smaller overall mass, and "heavier" isotopes (like the stable Carbon-13 isotope or radioactive Carbon-14 isotope) have a larger overall mass. Stable isotope geochemistry is concerned with how varying chemical and physical processes preferentially enrich or deplete stable isotopes. Enzymes like RuBisCO cause isotopic fractionation because molecules containing lighter isotopes have higher zero-point energies (ZPE), the lowest possible quantum energy
Hydrogen isotope biogeochemistry spectroscopic lines for publishable data, Murphy and Urey paired with Ferdinand Brickwedde and distilled a more concentrated pool of heavy hydrogen, known today as deuterium. This work on hydrogen isotopes won Urey the 1934 Nobel Prize in Chemistry. Also in 1934, scientists Ernest Rutherford, Mark Oliphant, and Paul Harteck, produced the radioactive isotope tritium by hitting deuterium with high energy nuclei. The deuterium used in the experiment was a generous gift of heavy water from the Berkeley physicist Gilbert N Lewis. Bombarding deuterium produced two previously undetected isotopes, helium-3 and hydrogen-3. Rutherford and his colleagues successfully created tritium, but incorrectly