Metamorphic rock Metamorphic rock Metamorphic rocks arise from the transformation of existing rock types, in a process called metamorphism, which means "change in form". The original rock (protolith) is subjected to heat (temperatures greater than 150 to 200 °C) and pressure ( or more), causing profound physical or chemical change. The protolith may be a sedimentary, igneous, or existing metamorphic rock. Metamorphic rocks make up a large part of the Earth's crust and form 12% of the Earth's land surface. They are classified by texture and by chemical and mineral assemblage (metamorphic facies). They may be formed simply by being deep beneath
Moist heat sterilization not achieved and the sterilization cycle may need revision or improvement. A more effective method is Tyndallization, which uses three successive steam treatments to achieve sterilization over the course of three days. This works by killing vegetative cells, allowing germination of surviving spores, and killing the resulting vegetative cells before they have time to form further spores. Any surviving spores from the first treatment, or incidentally formed spores during the first incubation period, are killed in a third steaming cycle. A more commonly used method when extended heat is not a concern is to use an autoclave or pressure cooker.