Kinetic Isotope Effects of RuBisCO carbon, oxygen, and/or hydrogen, though currently only a significant isotope effect is seen for carbon isotope substitution. Isotopes are atoms that have the same number of protons but varying numbers of neutrons. "Lighter" isotopes (like the stable Carbon-12 isotope) have a smaller overall mass, and "heavier" isotopes (like the stable Carbon-13 isotope or radioactive Carbon-14 isotope) have a larger overall mass. Stable isotope geochemistry is concerned with how varying chemical and physical processes preferentially enrich or deplete stable isotopes. Enzymes like RuBisCO cause isotopic fractionation because molecules containing lighter isotopes have higher zero-point energies (ZPE), the lowest possible quantum energy
Stable isotope ratio isotope fractionation in nature. This field is termed stable isotope geochemistry. Measurement of the ratios of naturally occurring stable isotopes (isotope analysis) plays an important role in isotope geochemistry, but stable isotopes (mostly carbon, nitrogen, oxygen and sulfur) are also finding uses in ecological and biological studies. Other workers have used oxygen isotope ratios to reconstruct historical atmospheric temperatures, making them important tools for paleoclimatology. These isotope systems for lighter elements that exhibit more than one primordial isotope for each element, have been under investigation for many years in order to study processes of isotope fractionation in natural systems. The