Kinetic Isotope Effects of RuBisCO carbon, oxygen, and/or hydrogen, though currently only a significant isotope effect is seen for carbon isotope substitution. Isotopes are atoms that have the same number of protons but varying numbers of neutrons. "Lighter" isotopes (like the stable Carbon-12 isotope) have a smaller overall mass, and "heavier" isotopes (like the stable Carbon-13 isotope or radioactive Carbon-14 isotope) have a larger overall mass. Stable isotope geochemistry is concerned with how varying chemical and physical processes preferentially enrich or deplete stable isotopes. Enzymes like RuBisCO cause isotopic fractionation because molecules containing lighter isotopes have higher zero-point energies (ZPE), the lowest possible quantum energy
Stable isotope ratio long history of study of these elements is in part because the proportions of stable isotopes in these light and volatile elements is relatively easy to measure. However, recent advances in isotope ratio mass spectrometry (i.e. multiple-collector inductively coupled plasma mass spectrometry) now enable the measurement of isotope ratios in heavier stable elements, such as iron, copper, zinc, molybdenum, etc. The variations in oxygen and hydrogen isotope ratios have applications in hydrology since most samples will lie between two extremes, ocean water and Arctic/Antarctic snow. Given a sample of water from an aquifer, and a sufficiently sensitive tool to measure