Hydrogen isotope biogeochemistry reactors in 1988, but nuclear weapons testing in the 1950s added large spikes of radioactive elements to the atmosphere, especially radiocarbon and tritium. This complicated measurements for geologists using radiometric dating of carbon. However, some oceanographers benefited from the tritium increase, utilizing the signal in the water to trace physical mixing of water masses. In biogeochemistry, scientists focused primarily on the stable isotope of deuterium as a tracer for environmental processes, especially the water cycle. The American geochemist Harmon Craig, once a graduate student of Urey's, discovered the relationship between rainwater's hydrogen and oxygen isotope ratios. The linear correlation between
Stable isotope labeling by amino acids in cell culture the stable isotopes. These small mass differences however need to be resolved on high resolution mass spectrometers. Stable isotope labeling by amino acids in cell culture Stable Isotope Labeling by/with Amino acids in Cell culture (SILAC) is a technique based on mass spectrometry that detects differences in protein abundance among samples using non-radioactive isotopic labeling. It is a popular method for quantitative proteomics. Two populations of cells are cultivated in cell culture. One of the cell populations is fed with growth medium containing normal amino acids. In contrast, the second population is fed with growth medium containing amino acids labeled