Nicolaes Tulp depicted in the painting can be dated to 16 January 1632; the Amsterdam Guild of Surgeons, of which Tulp was official City Anatomist, permitted only one public dissection a year and the body would have to be that of an executed criminal. The criminal is identified as the robber Aris Kindt. Rembrandt would later make a painting of Tulp's successor in 1656 "The Anatomy Lesson of Dr Jan Deijman". Since the painting of Tulp's predecessor in 1619, "The Osteology Lesson of Dr Sebastiaen Egbertsz" was a group portrait around a skeleton, it is clear that the subject of a dead
Nicolaes Tulp but also because of the fantastic stories that he relates. An example; Jan de Doot, a blacksmith in Amsterdam, was in such pain from a bladder stone, that he sharpened a knife and removed it himself, because he refused to be the victim of the 'stone cutters'. These were the barber-surgeons who performed such procedures but had a high death rate. To everyone's surprise, Jan de Doot survived this operation which was said to produce a stone the size of an egg. A painting illustrating this story is in the collection of the Anatomy Museum of Leiden. Tulp minutely described