Kinetic Isotope Effects of RuBisCO carbon, oxygen, and/or hydrogen, though currently only a significant isotope effect is seen for carbon isotope substitution. Isotopes are atoms that have the same number of protons but varying numbers of neutrons. "Lighter" isotopes (like the stable Carbon-12 isotope) have a smaller overall mass, and "heavier" isotopes (like the stable Carbon-13 isotope or radioactive Carbon-14 isotope) have a larger overall mass. Stable isotope geochemistry is concerned with how varying chemical and physical processes preferentially enrich or deplete stable isotopes. Enzymes like RuBisCO cause isotopic fractionation because molecules containing lighter isotopes have higher zero-point energies (ZPE), the lowest possible quantum energy
Isotope geochemistry this reason, enrichments are typically reported in "per mil" (‰, parts per thousand). These enrichments (δ) represent the ratio of heavy isotope to light isotope in the sample over the ratio of a standard. That is, Hydrogen isotope biogeochemistry Carbon has two stable isotopes, C and C, and one radioactive isotope, C. The stable carbon isotope ratio, "δ"C, is measured against Vienna Pee Dee Belemnite (VPDB). The stable carbon isotopes are fractionated primarily by photosynthesis (Faure, 2004). The C/C ratio is also an indicator of paleoclimate: a change in the ratio in the remains of plants indicates a change in