Radioactive tracer such as a cell or tissue, or as a flow tracer to track fluid flow. Radioactive tracers are also used to determine the location of fractures created by hydraulic fracturing in natural gas production. Radioactive tracers form the basis of a variety of imaging systems, such as, PET scans, SPECT scans and technetium scans. Radiocarbon dating uses the naturally occurring carbon-14 isotope as an isotopic label. Isotopes of a chemical element differ only in the mass number. For example, the isotopes of hydrogen can be written as H, H and H, with the mass number at top left. When the
Stable isotope labeling by amino acids in cell culture the stable isotopes. These small mass differences however need to be resolved on high resolution mass spectrometers. Stable isotope labeling by amino acids in cell culture Stable Isotope Labeling by/with Amino acids in Cell culture (SILAC) is a technique based on mass spectrometry that detects differences in protein abundance among samples using non-radioactive isotopic labeling. It is a popular method for quantitative proteomics. Two populations of cells are cultivated in cell culture. One of the cell populations is fed with growth medium containing normal amino acids. In contrast, the second population is fed with growth medium containing amino acids labeled