Kinetic Isotope Effects of RuBisCO carbon, oxygen, and/or hydrogen, though currently only a significant isotope effect is seen for carbon isotope substitution. Isotopes are atoms that have the same number of protons but varying numbers of neutrons. "Lighter" isotopes (like the stable Carbon-12 isotope) have a smaller overall mass, and "heavier" isotopes (like the stable Carbon-13 isotope or radioactive Carbon-14 isotope) have a larger overall mass. Stable isotope geochemistry is concerned with how varying chemical and physical processes preferentially enrich or deplete stable isotopes. Enzymes like RuBisCO cause isotopic fractionation because molecules containing lighter isotopes have higher zero-point energies (ZPE), the lowest possible quantum energy
Isotope separation isotopically purified elements are used in smaller quantities for specialist applications, especially in the semiconductor industry, where purified silicon is used to improve crystal structure and thermal conductivity, and carbon with greater isotopic purity to make diamonds with greater thermal conductivity. Isotope separation is an important process for both peaceful and military nuclear technology, and therefore the capability that a nation has for isotope separation is of extreme interest to the intelligence community. The only alternative to isotope separation is to manufacture the required isotope in its pure form. This may be done by irradiation of a suitable target, but