Kinetic Isotope Effects of RuBisCO carbon, oxygen, and/or hydrogen, though currently only a significant isotope effect is seen for carbon isotope substitution. Isotopes are atoms that have the same number of protons but varying numbers of neutrons. "Lighter" isotopes (like the stable Carbon-12 isotope) have a smaller overall mass, and "heavier" isotopes (like the stable Carbon-13 isotope or radioactive Carbon-14 isotope) have a larger overall mass. Stable isotope geochemistry is concerned with how varying chemical and physical processes preferentially enrich or deplete stable isotopes. Enzymes like RuBisCO cause isotopic fractionation because molecules containing lighter isotopes have higher zero-point energies (ZPE), the lowest possible quantum energy
Isotope analysis time, both are subject to post-depositional diagenesis. As such, isotopic analysis makes use of the more resistant phosphate groups, rather than the less abundant hydroxyl group or the more likely diagenetic carbonate groups present. Isotope analysis has widespread applicability in the natural sciences. These include numerous applications in the biological, earth and environmental sciences. Archaeological materials, such as bone, organic residues, hair, or sea shells, can serve as substrates for isotopic analysis. Carbon, nitrogen and zinc isotope ratios are used to investigate the diets of past people; These isotopic systems can be used with others, such as strontium or oxygen,