Nicolaes Tulp depicted in the painting can be dated to 16 January 1632; the Amsterdam Guild of Surgeons, of which Tulp was official City Anatomist, permitted only one public dissection a year and the body would have to be that of an executed criminal. The criminal is identified as the robber Aris Kindt. Rembrandt would later make a painting of Tulp's successor in 1656 "The Anatomy Lesson of Dr Jan Deijman". Since the painting of Tulp's predecessor in 1619, "The Osteology Lesson of Dr Sebastiaen Egbertsz" was a group portrait around a skeleton, it is clear that the subject of a dead
The Anatomy Lesson of Dr. Nicolaes Tulp copy of "The Anatomy Lesson of Dr Nicolaes Tulp" by an unknown artist hangs in Edinburgh as part of The University of Edinburgh Fine Art Collection. "The Gross Clinic" of 1875 and "The Agnew Clinic" of 1889 are paintings by the American artist Thomas Eakins which treat a similar subject, operations on live patients in the presence of medical students. In 2010, Yiull Damaso created a parody of the painting depicting prominent South Africans. Nelson Mandela was the cadaver, Nkosi Johnson was the instructor, and the students were Desmond Tutu, F. W. de Klerk, Thabo Mbeki, Jacob Zuma, Cyril Ramaphosa,