BCG vaccine fine powder. Sometimes the powder is sealed with vacuum in a glass ampoule. Such a glass ampoule has to be opened slowly to prevent the airflow from blowing out the powder. Then the powder has to be diluted with saline water before injecting. The history of BCG is tied to that of smallpox. Jean Antoine Villemin first recognized bovine tuberculosis in 1854 and transmitted it, and Robert Koch first distinguished "Mycobacterium bovis" from "Mycobacterium tuberculosis". Following the success of vaccination in preventing smallpox, established during the 18th century, scientists thought to find a corollary in tuberculosis by drawing a parallel
Inoculation theory of this study is to determine how to improve the effectiveness of the inoculation process by evaluating and generating reactance to a threatened freedom by manipulating explicit and implicit language and its intensity. While most inoculation studies focus on avoiding reactance, or at the very least, minimizing the impact of reactance on behaviors, in contrast, Miller, et al. (2013) chose to manipulate reactance by designing messages to enhance resistance and counterarguing output. They posit seven hypotheses in this study. H1 aims to discover if reactance-enhanced messages warning of a potential attitudinal threat negative affect and cognitions toward the attack message