BCG vaccine produce BCG. Along with supply availability from existing manufacturers, and a "new WHO prequalified vaccine" the total supply will be "sufficient to meet both suppressed 2015 demand carried over to 2016, as well as total forecast demand through 2016-2018." In the fall of 2011, the Sanofi Pasteur plant flooded causing problems with mold. The facility, located in Toronto, Ontario, Canada, produced BCG vaccine products, made with substrain Connaught, such as a tuberculosis vaccine ImmuCYST, a BCG Immunotherapeutic – a bladder cancer drug. By April 2012 the FDA had found dozens of documented problems with sterility at the plant including mold,
Inoculation needle above the sterile agar plate, the inoculation needle will be streaked across the plate in controlled directions. Microbial aerosols can be created from the hitting of the inoculation tip to the sides of the agar plate. The inoculation needle is then withdrawn from the inoculated agar plate culture and flamed. An inoculation needle is an essential tool in inoculating a stab culture. A sterile stab culture cap is removed and the open end is flamed. The needle tip and its length is then pushed into the stab media and stopped once the needle tip reaches 0.5 inches away from the