BCG vaccine bacilli that seemed less virulent, and changed the course of their research to see if repeated subculturing would produce a strain that was attenuated enough to be considered for use as a vaccine. The BCG strain was isolated after subculturing 239 times during 13 years from virulent strain on glycerine potato medium. The research continued throughout World War I until 1919, when the now avirulent bacilli were unable to cause tuberculosis disease in research animals. Calmette and Guerin transferred to the Paris Pasteur Institute in 1919. The BCG vaccine was first used in humans in 1921. Public acceptance was slow,
Inoculation theory a Mac or a Windows-based PC computer or if one should support gay marriage. Implementing inoculation theory in studies of contemporary social issues (from mundane to controversial social issues), the variety and resurgence of studies using inoculation theory helps bolster the effectiveness and utility of the theory and provides support that it can be used to strengthen and/or predict attitudes. McGuire led a series of experiments assessing inoculation's efficacy and adding nuance to our understanding for how it works (for a review, see Compton, 2013; Compton & Pfau, 2005). Early studies (e.g., McGuire & Papageorgis, 1961) limited testing of inoculation