BCG vaccine screened for active tuberculosis. BCG is also contraindicated in certain people who have IL-12 receptor pathway defects. BCG is given as a single intradermal injection at the insertion of the deltoid. If BCG is accidentally given subcutaneously, then a local abscess may form (a "BCG-oma") that can sometimes ulcerate, and may require treatment with antibiotics immediately, otherwise without treatment it could spread the infection causing severe damage to vital organs. However, it is important to note an abscess is not always associated with incorrect administration, and it is one of the more common complications that can occur with the vaccination.
Inoculation needle culture. Too much heat will kill off the inoculum during the direct contact of a flamed inoculation needle. The inoculation needle is withdrawn from the agar culture after obtaining a small colony and the agar plate lid is then replaced. Inoculation of the microorganisms will be done directly after the transfer from culture to inoculation needle. The inoculum is commonly inoculated to broth cultures, slant cultures, plate cultures, and stab cultures. An inoculation needle is used in inoculating a sterile broth culture. Flaming the open end of the broth will keep the it sterile. The broth will be moved up