BCG vaccine fine powder. Sometimes the powder is sealed with vacuum in a glass ampoule. Such a glass ampoule has to be opened slowly to prevent the airflow from blowing out the powder. Then the powder has to be diluted with saline water before injecting. The history of BCG is tied to that of smallpox. Jean Antoine Villemin first recognized bovine tuberculosis in 1854 and transmitted it, and Robert Koch first distinguished "Mycobacterium bovis" from "Mycobacterium tuberculosis". Following the success of vaccination in preventing smallpox, established during the 18th century, scientists thought to find a corollary in tuberculosis by drawing a parallel
Inoculation needle is uncapped. The open end of the uncapped slant culture is then flamed. The slant will be positioned to move up the needle until the inoculation needle tip lightly come into contact with the base surface of the sterile media. The inoculation needle inoculates the sterile agar by the manipulation of the media so that the needle tip grazes the agar surface in a zigzag pattern. Aseptic technique is then applied to the withdrawn inoculation needle. Inoculation of a plate culture is done through the streaking technique to make a streak plate. After lifting the lid so that it hovers