BCG vaccine nesting birds and rusted electrical conduits. The resulting closure of the plant for over two years resulting in shortages of bladder cancer and tuberculosis vaccines. On October 29, 2014 Health Canada gave the permission for Sanofi to resume production of BCG. The global supply situation was analyzed in 2017 and concluded that the global supply is sufficient to meet forecasted BCG vaccine demand but risks for shortages remain mainly due to supply concentration. A weakened strain of bovine tuberculosis bacillus, "Mycobacterium bovis" is specially subcultured in a culture medium, usually Middlebrook 7H9. Some BCG vaccines are freeze dried and become
Inoculation needle to the needle so that the needle tip is submerged while maintaining the needle's original position. Careful swirling of the needle can help the inoculation of the microorganism from the needle to the sterile broth. The inoculated broth culture is then removed from the needle. Aseptic technique is applied to the open end of the broth culture to prevent contaminants, and the needle is flamed for sterilization. An inoculation needle is used to inoculate a slant culture in a fish tail inoculation technique. After the microorganisms transfer from the original microbial culture to the inoculation needle the sterile slant culture