BCG vaccine of 2004 the vaccine is given to about 100 million children per year globally. The main use of BCG is for vaccination against tuberculosis. BCG vaccine can be administered after birth intradermally. BCG vaccination can cause a false positive Mantoux test, although a very high-grade reading is usually due to active disease. The most controversial aspect of BCG is the variable efficacy found in different clinical trials, which appears to depend on geography. Trials conducted in the UK have consistently shown a protective effect of 60 to 80%, but those conducted elsewhere have shown no protective effect, and efficacy appears
Inoculation needle culture. Too much heat will kill off the inoculum during the direct contact of a flamed inoculation needle. The inoculation needle is withdrawn from the agar culture after obtaining a small colony and the agar plate lid is then replaced. Inoculation of the microorganisms will be done directly after the transfer from culture to inoculation needle. The inoculum is commonly inoculated to broth cultures, slant cultures, plate cultures, and stab cultures. An inoculation needle is used in inoculating a sterile broth culture. Flaming the open end of the broth will keep the it sterile. The broth will be moved up