BCG vaccine produce BCG. Along with supply availability from existing manufacturers, and a "new WHO prequalified vaccine" the total supply will be "sufficient to meet both suppressed 2015 demand carried over to 2016, as well as total forecast demand through 2016-2018." In the fall of 2011, the Sanofi Pasteur plant flooded causing problems with mold. The facility, located in Toronto, Ontario, Canada, produced BCG vaccine products, made with substrain Connaught, such as a tuberculosis vaccine ImmuCYST, a BCG Immunotherapeutic – a bladder cancer drug. By April 2012 the FDA had found dozens of documented problems with sterility at the plant including mold,
Inoculation needle needle-oriented culture methods. The application of inoculation needles focuses on the inoculation and isolation of very defined regions of the cultures and the requirements of least disturbance between two closely crowded microbial colonies. It can also be used in harpooning under a low magnification microscope. Streaking on streak plates, fish tail inoculation of slant cultures and the inoculation of stab cultures can be done with the inoculation needle. Stab cultures specifically require the inoculation needle and is used to study cell motility, microbial oxygen requirements using Thioglycolate cultures, and the gelatin liquefaction of bacteria. The inoculation needle is sterilized using