BCG vaccine and one disaster, in particular, did much to harm public acceptance of the vaccine. In the summer of 1930 in Lübeck, 240 infants were vaccinated in the first 10 days of life; almost all developed tuberculosis and 72 infants died. It was subsequently discovered that the BCG administered there had been contaminated with a virulent strain that was being stored in the same incubator, which led to legal action against the manufacturers of the vaccine. Dr. R.G. Ferguson, working at the Fort Qu'Appelle Sanatorium in Saskatchewan, was among the pioneers in developing the practice of vaccination against tuberculosis. In 1928,
Inoculation needle culture. Too much heat will kill off the inoculum during the direct contact of a flamed inoculation needle. The inoculation needle is withdrawn from the agar culture after obtaining a small colony and the agar plate lid is then replaced. Inoculation of the microorganisms will be done directly after the transfer from culture to inoculation needle. The inoculum is commonly inoculated to broth cultures, slant cultures, plate cultures, and stab cultures. An inoculation needle is used in inoculating a sterile broth culture. Flaming the open end of the broth will keep the it sterile. The broth will be moved up