BCG vaccine bacilli that seemed less virulent, and changed the course of their research to see if repeated subculturing would produce a strain that was attenuated enough to be considered for use as a vaccine. The BCG strain was isolated after subculturing 239 times during 13 years from virulent strain on glycerine potato medium. The research continued throughout World War I until 1919, when the now avirulent bacilli were unable to cause tuberculosis disease in research animals. Calmette and Guerin transferred to the Paris Pasteur Institute in 1919. The BCG vaccine was first used in humans in 1921. Public acceptance was slow,
Inoculation theory "legalization of marijuana, gun control legislation, legalization of gambling, and restriction of television violence" (Miller et al., 2013, p. 135). Phase 2 presented an inoculation message relative to each participant's measured attitudes from Phase 1. The participants were divided into two groups, including the inoculation group and the control group. The questionnaire the participants received also gauged both their perceived threat and affect toward the attack messages. In Phase 3, each group was also given an attack message using either high-controlling or low-controlling language, and again, to evaluate the levels of perceived threat and affect. The study concluded that support