Spider told by the Roman poet Ovid in his "Metamorphoses", Arachne was a Lydian girl who challenged the goddess Athena to a weaving contest. Arachne won, but Athena destroyed her tapestry out of jealousy, causing Arachne to hang herself. In an act of mercy, Athena brought Arachne back to life as the first spider. Stories about the trickster-spider Anansi are prominent in the folk traditions of the Asante people of Ghana. In some cultures, spiders have symbolized patience due to their hunting technique of setting webs and waiting for prey, as well as mischief and malice due to their venomous bites.
Spider-Man: Into the Spider-Verse revealed to be voicing the character Spider-Man Noir, and John Mulaney and Kimiko Glenn were announced as voicing Spider-Ham and Peni Parker, respectively. The casting of Chris Pine as the Peter Parker of Miles' universe and Oscar Isaac as Spider-Man 2099 were revealed in November 2018. Lord and Miller explained that the alternate Spider-Man characters were chosen based on the comics they had read as well as research done on the Marvel Wiki, with the intention of including actual character from the comics who "were as diverse as possible". Lord and Miller wanted the film to feel like "you walked