BCG vaccine fine powder. Sometimes the powder is sealed with vacuum in a glass ampoule. Such a glass ampoule has to be opened slowly to prevent the airflow from blowing out the powder. Then the powder has to be diluted with saline water before injecting. The history of BCG is tied to that of smallpox. Jean Antoine Villemin first recognized bovine tuberculosis in 1854 and transmitted it, and Robert Koch first distinguished "Mycobacterium bovis" from "Mycobacterium tuberculosis". Following the success of vaccination in preventing smallpox, established during the 18th century, scientists thought to find a corollary in tuberculosis by drawing a parallel
Inoculation theory in South Dakota and had the students watch a video warning them of future pressures to smoke. In the first year, resistance was highest among those with low self-esteem (Pfau et al., 1992). At the end of the second year, students in the group showed more attitudinal resistance to smoking than they did previously (Pfau & Van Bockern 1994). Importantly, the study and its follow-up demonstrate the long-lasting effects of inoculation treatments. Other recent areas of study include vaccination safety. Wong and Harrison (2014) studied 212 female students and their attitudes about the HPV vaccine. They found that inoculation messages