BCG vaccine bacilli that seemed less virulent, and changed the course of their research to see if repeated subculturing would produce a strain that was attenuated enough to be considered for use as a vaccine. The BCG strain was isolated after subculturing 239 times during 13 years from virulent strain on glycerine potato medium. The research continued throughout World War I until 1919, when the now avirulent bacilli were unable to cause tuberculosis disease in research animals. Calmette and Guerin transferred to the Paris Pasteur Institute in 1919. The BCG vaccine was first used in humans in 1921. Public acceptance was slow,
Inoculation theory regarding the impact of gender on inoculation effectiveness in issue/advocacy campaigns. The conclusion was made that females are more likely to be inoculated with these types of campaigns. An additional observation made by Burgoon et. al (1995) was that the type of content used in these campaigns contributed to the campaigns success. The further the advertisement was from "direct self-benefit" the greater the inoculation effect was on the audience (Burgoon, et al., 1995). It took quite some time for inoculation theory to be applied to marketing, because of the many limitations (Lessne and Didow, 1987). Lessne and Didow (1987) reviewed