BCG vaccine bacilli that seemed less virulent, and changed the course of their research to see if repeated subculturing would produce a strain that was attenuated enough to be considered for use as a vaccine. The BCG strain was isolated after subculturing 239 times during 13 years from virulent strain on glycerine potato medium. The research continued throughout World War I until 1919, when the now avirulent bacilli were unable to cause tuberculosis disease in research animals. Calmette and Guerin transferred to the Paris Pasteur Institute in 1919. The BCG vaccine was first used in humans in 1921. Public acceptance was slow,
A2261-BCG A2261-BCG A2261-BCG (short for Abell 2261 Brightest Cluster Galaxy) is a huge elliptical galaxy in the cluster Abell 2261. One of the largest galaxies known, A2261-BCG is estimated to have a diameter of a million light-years, some 10 times larger than the Milky Way. It is the brightest and the most massive galaxy in the cluster, and has the largest galactic core ever observed, spanning more than 10,000 light-years. The cD elliptical galaxy, located at least 3 billion light-years from Earth, is also well known as a radio source. Its core is highly populated by a dense number of old