BCG vaccine BCG vaccine Bacillus Calmette–Guérin (BCG) vaccine is a vaccine primarily used against tuberculosis (TB). In countries where tuberculosis or leprosy is common, one dose is recommended in healthy babies as close to the time of birth as possible. In areas where tuberculosis is not common, only children at high risk are typically immunized, while suspected cases of tuberculosis are individually tested for and treated. Adults who do not have tuberculosis and have not been previously immunized but are frequently exposed may be immunized as well. BCG also has some effectiveness against Buruli ulcer infection and other nontuberculous mycobacteria infections. Additionally
Inoculation needle to the needle so that the needle tip is submerged while maintaining the needle's original position. Careful swirling of the needle can help the inoculation of the microorganism from the needle to the sterile broth. The inoculated broth culture is then removed from the needle. Aseptic technique is applied to the open end of the broth culture to prevent contaminants, and the needle is flamed for sterilization. An inoculation needle is used to inoculate a slant culture in a fish tail inoculation technique. After the microorganisms transfer from the original microbial culture to the inoculation needle the sterile slant culture