BCG vaccine bacilli that seemed less virulent, and changed the course of their research to see if repeated subculturing would produce a strain that was attenuated enough to be considered for use as a vaccine. The BCG strain was isolated after subculturing 239 times during 13 years from virulent strain on glycerine potato medium. The research continued throughout World War I until 1919, when the now avirulent bacilli were unable to cause tuberculosis disease in research animals. Calmette and Guerin transferred to the Paris Pasteur Institute in 1919. The BCG vaccine was first used in humans in 1921. Public acceptance was slow,
Inoculation theory motivation to maintain current attitudes and beliefs, but weak enough that the receiver will refute the counterargument (Compton, 2013; McGuire, 1964). Inoculation has been proven successful through trials and research. This article highlights vital aspects of inoculation theory, however, it is not exhaustive–there is a large volume of quality research on the theory not mentioned. Finally, while numerous studies have tested and verified the efficacy of the inoculation process, there continues to be a need for refinement and elaboration. Inoculation theory is used today as part of the suite of tools used by those engaged in shaping or manipulating public