BCG vaccine bacilli that seemed less virulent, and changed the course of their research to see if repeated subculturing would produce a strain that was attenuated enough to be considered for use as a vaccine. The BCG strain was isolated after subculturing 239 times during 13 years from virulent strain on glycerine potato medium. The research continued throughout World War I until 1919, when the now avirulent bacilli were unable to cause tuberculosis disease in research animals. Calmette and Guerin transferred to the Paris Pasteur Institute in 1919. The BCG vaccine was first used in humans in 1921. Public acceptance was slow,
Inoculation theory field itself is meant to act to the public, their opinions, and their actions. It is especially useful with an audience who already has an opinion on a brand. Inoculation theory is the perfect way to convince already faithful customers that they are making the right choice in trusting your company and to keep the customer coming back in the future. For example, Apple and their "Get A Mac" campaign. This campaign did a great job of following the inoculation theory in targeting those who already preferred Mac computers. The series of ads pit out in the duration of the