BCG vaccine extensively used even in countries where efficacy against pulmonary tuberculosis is negligible. A number of possible reasons for the variable efficacy of BCG in different countries have been proposed. None have been proven, some have been disproved, and none can explain the lack of efficacy in both low-TB burden countries (US) and high-TB burden countries (India). The reasons for variable efficacy have been discussed at length in a WHO document on BCG. BCG has protective effects against some non-tuberculosis mycobacteria. BCG has been one of the most successful immunotherapies. BCG vaccine has been the "standard of care for patients with
Inoculation needle culture. Too much heat will kill off the inoculum during the direct contact of a flamed inoculation needle. The inoculation needle is withdrawn from the agar culture after obtaining a small colony and the agar plate lid is then replaced. Inoculation of the microorganisms will be done directly after the transfer from culture to inoculation needle. The inoculum is commonly inoculated to broth cultures, slant cultures, plate cultures, and stab cultures. An inoculation needle is used in inoculating a sterile broth culture. Flaming the open end of the broth will keep the it sterile. The broth will be moved up