BCG vaccine nesting birds and rusted electrical conduits. The resulting closure of the plant for over two years resulting in shortages of bladder cancer and tuberculosis vaccines. On October 29, 2014 Health Canada gave the permission for Sanofi to resume production of BCG. The global supply situation was analyzed in 2017 and concluded that the global supply is sufficient to meet forecasted BCG vaccine demand but risks for shortages remain mainly due to supply concentration. A weakened strain of bovine tuberculosis bacillus, "Mycobacterium bovis" is specially subcultured in a culture medium, usually Middlebrook 7H9. Some BCG vaccines are freeze dried and become
Inoculation needle above the sterile agar plate, the inoculation needle will be streaked across the plate in controlled directions. Microbial aerosols can be created from the hitting of the inoculation tip to the sides of the agar plate. The inoculation needle is then withdrawn from the inoculated agar plate culture and flamed. An inoculation needle is an essential tool in inoculating a stab culture. A sterile stab culture cap is removed and the open end is flamed. The needle tip and its length is then pushed into the stab media and stopped once the needle tip reaches 0.5 inches away from the