BCG vaccine produce BCG. Along with supply availability from existing manufacturers, and a "new WHO prequalified vaccine" the total supply will be "sufficient to meet both suppressed 2015 demand carried over to 2016, as well as total forecast demand through 2016-2018." In the fall of 2011, the Sanofi Pasteur plant flooded causing problems with mold. The facility, located in Toronto, Ontario, Canada, produced BCG vaccine products, made with substrain Connaught, such as a tuberculosis vaccine ImmuCYST, a BCG Immunotherapeutic – a bladder cancer drug. By April 2012 the FDA had found dozens of documented problems with sterility at the plant including mold,
Inoculation needle the aseptic technique. An open flame from an incinerator, a bunsen burner, or an alcohol burner is used to flame along the tip and the length of the needle that is to be in contact with the inoculum. For ease of manipulation it is common practice to hold the needle with the dominant hand as if handling a pencil. The needle will be flamed at a downward angle through the flame’s inner cone until it is red-hot. The downward angle will minimize the amount of microbial aerosols created. Inoculation needles must be sterilized prior and following contact with microbial life