BCG vaccine and one disaster, in particular, did much to harm public acceptance of the vaccine. In the summer of 1930 in Lübeck, 240 infants were vaccinated in the first 10 days of life; almost all developed tuberculosis and 72 infants died. It was subsequently discovered that the BCG administered there had been contaminated with a virulent strain that was being stored in the same incubator, which led to legal action against the manufacturers of the vaccine. Dr. R.G. Ferguson, working at the Fort Qu'Appelle Sanatorium in Saskatchewan, was among the pioneers in developing the practice of vaccination against tuberculosis. In 1928,
Inoculation needle is uncapped. The open end of the uncapped slant culture is then flamed. The slant will be positioned to move up the needle until the inoculation needle tip lightly come into contact with the base surface of the sterile media. The inoculation needle inoculates the sterile agar by the manipulation of the media so that the needle tip grazes the agar surface in a zigzag pattern. Aseptic technique is then applied to the withdrawn inoculation needle. Inoculation of a plate culture is done through the streaking technique to make a streak plate. After lifting the lid so that it hovers