BCG vaccine bacilli that seemed less virulent, and changed the course of their research to see if repeated subculturing would produce a strain that was attenuated enough to be considered for use as a vaccine. The BCG strain was isolated after subculturing 239 times during 13 years from virulent strain on glycerine potato medium. The research continued throughout World War I until 1919, when the now avirulent bacilli were unable to cause tuberculosis disease in research animals. Calmette and Guerin transferred to the Paris Pasteur Institute in 1919. The BCG vaccine was first used in humans in 1921. Public acceptance was slow,
Abell 1201 BCG than expected from the usual scaling relations between black holes and host galaxies. However, alternative methods of modelling the stellar velocity dispersion maps reveals an ambiguity between the UMBH mass and the dark halo profile. In solutions where the UMBH is more massive, the dark matter is more cuspy. In solutions where the UMBH is smaller or absent, the dark matter is more cored. The dark halo's ellipticity and the mass-to-light ratio of stars also enter the ambiguity. Thus, if the standard computational models are robust, then Abell 1201 BCG presents a dilemma and a challenge to either the conventional