BCG vaccine produce BCG. Along with supply availability from existing manufacturers, and a "new WHO prequalified vaccine" the total supply will be "sufficient to meet both suppressed 2015 demand carried over to 2016, as well as total forecast demand through 2016-2018." In the fall of 2011, the Sanofi Pasteur plant flooded causing problems with mold. The facility, located in Toronto, Ontario, Canada, produced BCG vaccine products, made with substrain Connaught, such as a tuberculosis vaccine ImmuCYST, a BCG Immunotherapeutic – a bladder cancer drug. By April 2012 the FDA had found dozens of documented problems with sterility at the plant including mold,
Inoculation needle forms to ensure no contamination of the culture, the sterile mediums, and the surrounding environment. In inoculation the inoculation needle is first employed to transfer microbial life forms from a culture to the needle to be used in further inoculating procedures. Sources of inoculum include broth cultures, slant cultures, and plate cultures. An inoculation needle is used in the transfer of microorganisms from a broth culture by first employing a vortex mixer to ensure a uniform suspension of the microorganisms within the broth. Aseptic technique is then applied to the needle. Once the needle is flamed and sterilized the culture