BCG vaccine produce BCG. Along with supply availability from existing manufacturers, and a "new WHO prequalified vaccine" the total supply will be "sufficient to meet both suppressed 2015 demand carried over to 2016, as well as total forecast demand through 2016-2018." In the fall of 2011, the Sanofi Pasteur plant flooded causing problems with mold. The facility, located in Toronto, Ontario, Canada, produced BCG vaccine products, made with substrain Connaught, such as a tuberculosis vaccine ImmuCYST, a BCG Immunotherapeutic – a bladder cancer drug. By April 2012 the FDA had found dozens of documented problems with sterility at the plant including mold,
Inoculation needle to the needle so that the needle tip is submerged while maintaining the needle's original position. Careful swirling of the needle can help the inoculation of the microorganism from the needle to the sterile broth. The inoculated broth culture is then removed from the needle. Aseptic technique is applied to the open end of the broth culture to prevent contaminants, and the needle is flamed for sterilization. An inoculation needle is used to inoculate a slant culture in a fish tail inoculation technique. After the microorganisms transfer from the original microbial culture to the inoculation needle the sterile slant culture