Tuberculosis diagnosis by BCG inoculation tuberculin tests can be affected. In general the US approach is likely to result in more false positives and more unnecessary treatment with potentially toxic drugs; the UK approach is as sensitive in theory and should also be more specific, because of the use of interferon-γ tests. Under the US recommendations, diagnosis and treatment of latent tuberculosis infection (LTBI) is considered for any BCG-vaccinated person whose skin test is 10 mm or greater, if any of these circumstances are present: These have been reviewed in detail. In 2007, a systematic review of adenosine deaminase by the NHS
Inoculation needle the aseptic technique. An open flame from an incinerator, a bunsen burner, or an alcohol burner is used to flame along the tip and the length of the needle that is to be in contact with the inoculum. For ease of manipulation it is common practice to hold the needle with the dominant hand as if handling a pencil. The needle will be flamed at a downward angle through the flame’s inner cone until it is red-hot. The downward angle will minimize the amount of microbial aerosols created. Inoculation needles must be sterilized prior and following contact with microbial life