BCG vaccine bacilli that seemed less virulent, and changed the course of their research to see if repeated subculturing would produce a strain that was attenuated enough to be considered for use as a vaccine. The BCG strain was isolated after subculturing 239 times during 13 years from virulent strain on glycerine potato medium. The research continued throughout World War I until 1919, when the now avirulent bacilli were unable to cause tuberculosis disease in research animals. Calmette and Guerin transferred to the Paris Pasteur Institute in 1919. The BCG vaccine was first used in humans in 1921. Public acceptance was slow,
Belarusian Railway BCG-1 Belarusian Railway BCG-1 The Belarusian Railways BCG-1, originally Belarusian Railways BKG-1 (БКГ : "Белорусско-Китайский Грузовой"), is a type of twin-unit, eight axle, (Bo'Bo')-(Bo'Bo'), high power mainline electric freight locomotive manufactured by CNR Datong Electric Locomotive for the Belarusian Railway. The locomotives are derived from the China Railways HXD2 locomotive type developed from the Alstom Prima locomotive platform. In April 2010 CNR Datong announced that it had obtained a 700million Yuan contract to supply 12 twin section electric locomotives to Belarus; the locomotives were to be used to increase haulage capacity from the 4700t to 5500t capacity of the 6.52MW VL80S