BCG vaccine bacilli that seemed less virulent, and changed the course of their research to see if repeated subculturing would produce a strain that was attenuated enough to be considered for use as a vaccine. The BCG strain was isolated after subculturing 239 times during 13 years from virulent strain on glycerine potato medium. The research continued throughout World War I until 1919, when the now avirulent bacilli were unable to cause tuberculosis disease in research animals. Calmette and Guerin transferred to the Paris Pasteur Institute in 1919. The BCG vaccine was first used in humans in 1921. Public acceptance was slow,
Inoculation theory an individual. Future studies in the area of health can be extremely beneficial to communities. Some areas that are currently being researched can possibly help with present-day issues (e.g., inoculation-based strategies for addiction intervention to assist sober individuals from relapsing). Other areas for future studies include, promoting healthy eating habits, exercising, breastfeeding and creating positive attitude towards mammograms (Ivanov, 2017). An area that has been underdeveloped is mental health awareness. With the number of young adults and teens committing suicide due to bullying this can be an area where inoculation messages could be effective. McGuire (1961) used a medical analogy