BCG vaccine extensively used even in countries where efficacy against pulmonary tuberculosis is negligible. A number of possible reasons for the variable efficacy of BCG in different countries have been proposed. None have been proven, some have been disproved, and none can explain the lack of efficacy in both low-TB burden countries (US) and high-TB burden countries (India). The reasons for variable efficacy have been discussed at length in a WHO document on BCG. BCG has protective effects against some non-tuberculosis mycobacteria. BCG has been one of the most successful immunotherapies. BCG vaccine has been the "standard of care for patients with
Inoculation needle hand immobile. In slant culture transfer, the inoculation needle will be first treated with the aseptic technique by flaming. The slant culture cap is then removed and secured using the needle hand. Flaming the open end of the slant culture will prevent contamination and the formation of aerosols. Transfer happens once the tip of the inoculation needle comes into contact with the agar surface of the slant culture. The inoculation needle should not move in the process. The agar slant culture will be moved up to the needle to prevent jostling of the needle. Once the transfer is complete from