BCG vaccine screened for active tuberculosis. BCG is also contraindicated in certain people who have IL-12 receptor pathway defects. BCG is given as a single intradermal injection at the insertion of the deltoid. If BCG is accidentally given subcutaneously, then a local abscess may form (a "BCG-oma") that can sometimes ulcerate, and may require treatment with antibiotics immediately, otherwise without treatment it could spread the infection causing severe damage to vital organs. However, it is important to note an abscess is not always associated with incorrect administration, and it is one of the more common complications that can occur with the vaccination.
Inoculation needle forms to ensure no contamination of the culture, the sterile mediums, and the surrounding environment. In inoculation the inoculation needle is first employed to transfer microbial life forms from a culture to the needle to be used in further inoculating procedures. Sources of inoculum include broth cultures, slant cultures, and plate cultures. An inoculation needle is used in the transfer of microorganisms from a broth culture by first employing a vortex mixer to ensure a uniform suspension of the microorganisms within the broth. Aseptic technique is then applied to the needle. Once the needle is flamed and sterilized the culture