Tabula (game) Tabula were reconstructed in the 19th century by Becq de Fouquières based upon this epigram. The game was played on a board nearly identical to a modern backgammon board with 24 points, 12 on each side. Two players had 15 pieces each, and moved them in opposing directions around the board, according to the roll of three dice. A piece resting alone in a space on the board was vulnerable to being hit. Hitting a blot, reentering a piece from the bar, and bearing off, all had the same rules as today. The only differences with modern backgammon were the
Western blot normalization the blot evenly; more stain might collect towards the edges of the blot than in the center. Non-uniformity in the image can result in inaccurate normalization. Anionic dyes such as Ponceau S and Coomassie Brilliant Blue, and fluorescent dyes like Sypro Ruby and Deep Purple, are used before antibodies are added because they do not affect downstream imumunodetection. Ponceau S is a negatively charged reversible dye that stains proteins a reddish pink color and is removed easily by washing in water. The intensity of Ponceau S staining decreases quickly over time, so documentation should be conducted rapidly. A linear range