Nicholas Throckmorton parish church, Leadenhall Street, London. Throckmorton married Anne Carew, daughter of Sir Nicholas Carew, a Knight of the Garter and his wife Elizabeth Bryan, and they had 10 sons and three daughters. Their daughter Elizabeth became the wife of Sir Walter Raleigh. After his death, Anne married Adrian Stokes, the second husband, and former Master of Horse of, Frances Brandon, Duchess of Suffolk. Contemporary political figures regarded Throckmorton with respect. One of these was Sir Francis Walsingham who had worked with Throckmorton in France. In 1560 William Cecil said he would be prepared to resign if Throckmorton would take his
Nicholas Throckmorton successor to Elizabeth, so he was an unwelcome guest. Some of Elizabeth's messages also offended the lords. Throckmorton was recalled in August after he offended Elizabeth by showing his instructions to the Scottish lords. In 1569, Throckmorton was suspected of involvement in the Duke of Norfolk's conspiracy in favour of Mary, and was imprisoned for a time at Windsor. Throckmorton may have erroneously believed Norfolk's idea would suit the wishes of the Queen. He was not put to trial, but did not regain the Queen's confidence afterwards. Throckmorton died on 12 February 1571. He is buried in St Katharine Cree