Raleigh's El Dorado Expedition Portuguese possessions and shipping, and conduct illicit trading. Sir Walter Raleigh had enjoyed several years of high esteem from Queen Elizabeth I, which stemmed in part from his previous exploits at sea which included the famous Capture of the Madre de Deus. Soon after, however, Raleigh suffered a short imprisonment for secretly marrying one of the Queen's ladies-in-waiting, Elizabeth Throckmorton, and bearing her a child. In a bid to restore his influence with the Queen, Raleigh, having promised a "gold-rich empire more lucrative than Peru", had set up an expedition under John Whiddon to find the fabled city of gold
Elizabeth Knollys sister. Elizabeth Knollys was born on 15 June 1549, the second daughter and one of the 15 children of Sir Francis Knollys and Catherine Carey, the daughter of William Carey and Mary Boleyn. This made Elizabeth the grand-niece of Queen Anne Boleyn, second wife of Henry VIII. She had 11 surviving siblings, the eldest of whom, Lettice Knollys, would later be banished from court after secretly marrying Queen Elizabeth's favourite, Robert Dudley, 1st Earl of Leicester. She was brought up in a staunchly Protestant household at Greys Court at Rotherfield Greys in Oxfordshire and Abbey House at Reading in Berkshire.