Nicholas Throckmorton parish church, Leadenhall Street, London. Throckmorton married Anne Carew, daughter of Sir Nicholas Carew, a Knight of the Garter and his wife Elizabeth Bryan, and they had 10 sons and three daughters. Their daughter Elizabeth became the wife of Sir Walter Raleigh. After his death, Anne married Adrian Stokes, the second husband, and former Master of Horse of, Frances Brandon, Duchess of Suffolk. Contemporary political figures regarded Throckmorton with respect. One of these was Sir Francis Walsingham who had worked with Throckmorton in France. In 1560 William Cecil said he would be prepared to resign if Throckmorton would take his
Elizabeth I of England for a smooth succession. Since Elizabeth would never name her successor, Cecil was obliged to proceed in secret. He therefore entered into a coded negotiation with James VI of Scotland, who had a strong but unrecognised claim. Cecil coached the impatient James to humour Elizabeth and "secure the heart of the highest, to whose sex and quality nothing is so improper as either needless expostulations or over much curiosity in her own actions". The advice worked. James's tone delighted Elizabeth, who responded: "So trust I that you will not doubt but that your last letters are so acceptably taken as