Tabula (game) Tabula were reconstructed in the 19th century by Becq de Fouquières based upon this epigram. The game was played on a board nearly identical to a modern backgammon board with 24 points, 12 on each side. Two players had 15 pieces each, and moved them in opposing directions around the board, according to the roll of three dice. A piece resting alone in a space on the board was vulnerable to being hit. Hitting a blot, reentering a piece from the bar, and bearing off, all had the same rules as today. The only differences with modern backgammon were the
Reverse northern blot by RNAses or tissue metabolites. Prepared reverse northern blot membranes are pre-hybridized in Denhardt's solution with SSC buffer and labeled cDNA probes are denatured at 100 °C and added to the pre-hybridization solution. The membrane is incubated with the probes for at least 15 hours at 65 °C, then washed and exposed. Reverse Northern blot, much like the northern blot upon which it is based, is used to determine levels of gene expression in particular tissues. In comparison to the Northern blot, the reverse northern blot is able to probe a large number of transcripts at once with less specificity