Patronages of the Immaculate Conception the Immaculate Conception is also one of the nine national and public holidays and a holy day of obligation. The patronage settles the dispute between a heated religious debate during the mid 1600s, when followers of the mythical figure Saint James Matamoros wished to impose him as the national patron saint versus the followers of Saint Teresa of Avila. On 11 September 1759, the elected members of the Spanish Cortes petitioned King Charles III of Spain to request from the pope the "universal patronage of Our Lady of the Immaculate Conception in all the Kingdoms of Spain and the Indies".
The Vision of Saint Eustace the fact that Pisanello painted Saint Eustace in Sant'Anastasia. Saint Hubert’s depiction in Italian art during this period is also unlikely. Saint Eustace is portrayed as a huntsman dressed in the height of court fashion, wearing a golden tunic and blue headdress. The patron was therefore given the opportunity to appreciate the work as a devotional piece in addition to identifying with the aristocratic pastime of hunting and chivalric ideology. The patron for whom the work was painted is, however, unknown. Suggested patrons include the Gonzaga and Filippo Maria Visconti. Leonello or Borso d’Este, keen huntsmen, have also been proposed.