Malachite green is retained in catfish muscle longer (HL = 10 days) than is the parent molecule (HL = 2.8 days). The (oral, mouse) is 80 mg/kg. Rats fed malachite green experience "a dose-related increase in liver DNA adducts" along with lung adenomas. Leucomalachite green causes an "increase in the number and severity of changes". As leucomalachite green is the primary metabolite of malachite green and is retained in fish muscle much longer, most human dietary intake of malachite green from eating fish would be in the leuco form. During the experiment, rats were fed up to 543 ppm of leucomalachite green,
Italian submarine Malachite in Cagliari, when already in view of the Sardinian coast she was spotted by the Dutch submarine HNLMS Dolfijn in the morning of February 9, 1943. At approximately 11:00, HNMS Dolfijn launched a salvo of 4 torpedoes at "Malachite". The Italian submarine managed to avoid three of them by deftly maneuvering, but the fourth one hit her on the stern, and she sank within a minute taking down 35 crew with her. Commander Cinti, three other officers, and nine NCOs and sailors managed to escape. The wreck of "Malachite" was located in September 1999 at a depth of 117 to