Pharaoh ant new nest is formed, queens are not a necessity; workers can raise new queens after finding a suitable nest site. In pharaoh ant colonies new males and queen ants can be produced when the existing fertile queen is removed. When queens are absent, the workers in the nest can do two things: either rear existing sexual larvae or transport sexual larvae from other bud nests or from the main nest to its own nest. However, when there are fertile queens still within the nest, the worker ants will cannibalize the sexual larvae and will either reject or consume sexual larvae
Leafcutter ant virulent fungus, has the potential to devastate an ant garden, as it is horizontally transmitted. "Escovopsis" was cultured, during colony foundation, in 6.6% of colonies. However, in one- to two-year-old colonies, almost 60% had "Escovopsis" growing in the fungal garden. In some parts of their range, leafcutter ants can be a serious agricultural pest, defoliating crops and damaging roads and farmland with their nest-making activities. For example, some "Atta" species are capable of defoliating an entire citrus tree in less than 24 hours. A promising approach to deterring attacks of the leafcutter ant "Acromyrmex lobicornis" on crops has been demonstrated.