Malachite green is retained in catfish muscle longer (HL = 10 days) than is the parent molecule (HL = 2.8 days). The (oral, mouse) is 80 mg/kg. Rats fed malachite green experience "a dose-related increase in liver DNA adducts" along with lung adenomas. Leucomalachite green causes an "increase in the number and severity of changes". As leucomalachite green is the primary metabolite of malachite green and is retained in fish muscle much longer, most human dietary intake of malachite green from eating fish would be in the leuco form. During the experiment, rats were fed up to 543 ppm of leucomalachite green,
The Malachite Box underdeveloped, at that time there were no specific criteria of distinguishing literature from folklore. The question of authorship arose every time a new story was released, and is still discussed nowadays. Before the stories were released as a single volume on 28 January 1939, the issue became urgent. The publisher was concerned with the title of the book and the author's name. It was suggested to release the book as "The Tales from Grandpa Slyshko" (), as if Vasily Hmelinin was the author. It was eventually published as "The Malachite Box". Bazhov was not certain of the authorship himself. He