Ultraviolet photography Silicon (from which DSLR sensors are made) can respond to wavelengths between 1100-190 nm. Photography based on visible fluorescence induced by UV radiation uses the same ultraviolet illumination as in reflected UV photography. However, the glass barrier filter used on the lens must now absorb or block all ultraviolet and infrared light and must permit only the "visible" radiation to pass. Visible fluorescence is produced in a suitable subject when the shorter, higher energy ultraviolet wavelengths are absorbed, lose some energy and are emitted as longer, lower energy visible wavelengths. UV induced visible fluorescence photography must take place in a
Ultraviolet germicidal irradiation fluence and it is assumed that the UV fluence will be equal to the UV dose. The UV dose is the amount of germicidal UV energy absorbed by a microbial population over a period of time. If the microorganisms are planktonic (free floating) the UV fluence will be equal the UV dose. However, if the microorganisms are protected by mechanical particles, such as dust and dirt, or have formed biofilm a much higher UV fluence will be needed for an effective UV dose to be introduced to the microbial population. The degree of inactivation by ultraviolet radiation is directly related