Polyacrylamide gel electrophoresis the top to create the sample wells. After the gel is polymerized the comb can be removed and the gel is ready for electrophoresis. Various buffer systems are used in PAGE depending on the nature of the sample and the experimental objective. The buffers used at the anode and cathode may be the same or different. An electric field is applied across the gel, causing the negatively charged proteins or nucleic acids to migrate across the gel away from the negative electrode (which is the cathode being that this is an electrolytic rather than galvanic cell) and towards the positive
Electrode for advanced purposes in research and investigation. Electrode An electrode is an electrical conductor used to make contact with a nonmetallic part of a circuit (e.g. a semiconductor, an electrolyte, a vacuum or air). The word was coined by William Whewell at the request of the scientist Michael Faraday from two Greek words: "elektron", meaning amber (from which the word electricity is derived), and "hodos", a way. The electrophore, invented by Johan Wilcke, was an early version of an electrode used to study static electricity. An electrode in an electrochemical cell is referred to as either an "anode" or a