Polyacrylamide gel electrophoresis the top to create the sample wells. After the gel is polymerized the comb can be removed and the gel is ready for electrophoresis. Various buffer systems are used in PAGE depending on the nature of the sample and the experimental objective. The buffers used at the anode and cathode may be the same or different. An electric field is applied across the gel, causing the negatively charged proteins or nucleic acids to migrate across the gel away from the negative electrode (which is the cathode being that this is an electrolytic rather than galvanic cell) and towards the positive
Fluoride selective electrode Fluoride selective electrode A fluoride selective electrode is a type of ion selective electrode sensitive to the concentration of the fluoride ion. A common example is the lanthanum fluoride electrode. In the lanthanum fluoride electrode, the sensing element is a crystal of lanthanum fluoride LaF, doped with europium fluoride EuF to create lattice vacancies. Such a crystal is an ionic conductor by virtue of the mobility of fluoride ions which jump between lattice vacancies. An electrochemical cell may be constructed using such a crystal as a membrane separating two fluoride solutions. This cell acts as a concentration cell with transference