Polyacrylamide gel electrophoresis the top to create the sample wells. After the gel is polymerized the comb can be removed and the gel is ready for electrophoresis. Various buffer systems are used in PAGE depending on the nature of the sample and the experimental objective. The buffers used at the anode and cathode may be the same or different. An electric field is applied across the gel, causing the negatively charged proteins or nucleic acids to migrate across the gel away from the negative electrode (which is the cathode being that this is an electrolytic rather than galvanic cell) and towards the positive
Plasma electrolytic oxidation consisting of the wall of the bath itself. Potentials of over 200 V are applied between these two electrodes. These may be continuous or pulsed direct current (DC) (in which case the part is simply an anode in DC operation), or alternating pulses (alternating current or "pulsed bi-polar" operation) where the stainless steel counter electrode might just be earthed. One of the remarkable features of plasma electrolyte coatings is the presence of micro pores and cracks on the coating surface. Plasma electrolytic oxide coatings are generally recognized for high hardness, wear resistance, and corrosion resistance. However, the coating properties are