Polyacrylamide gel electrophoresis the top to create the sample wells. After the gel is polymerized the comb can be removed and the gel is ready for electrophoresis. Various buffer systems are used in PAGE depending on the nature of the sample and the experimental objective. The buffers used at the anode and cathode may be the same or different. An electric field is applied across the gel, causing the negatively charged proteins or nucleic acids to migrate across the gel away from the negative electrode (which is the cathode being that this is an electrolytic rather than galvanic cell) and towards the positive
Plate electrode tube envelope and is cooled by radiation cooling, forced air or water. A problem in early vacuum tubes was "secondary emission"; electrons striking the plate could knock other electrons out of the metal surface. In some tubes such as tetrodes these "secondary electrons" could be absorbed by other electrods such as grids in the tube, resulting in a current out of the plate. This current could cause the plate circuit to have negative resistance, which could cause unwanted parasitic oscillations. To prevent this most plates in modern tubes are given a chemical coating which reduces secondary emission. Plate electrode A