Polyacrylamide gel electrophoresis the top to create the sample wells. After the gel is polymerized the comb can be removed and the gel is ready for electrophoresis. Various buffer systems are used in PAGE depending on the nature of the sample and the experimental objective. The buffers used at the anode and cathode may be the same or different. An electric field is applied across the gel, causing the negatively charged proteins or nucleic acids to migrate across the gel away from the negative electrode (which is the cathode being that this is an electrolytic rather than galvanic cell) and towards the positive
Concentration polarization Concentration polarization Concentration polarization is a term used in the scientific fields of electrochemistry and membrane science. In electrochemistry, concentration polarization denotes the part of the polarization of an electrolytic cell resulting from changes in the electrolyte concentration due to the passage of current through the electrode/solution interface. Here "polarization" is understood as the shift of the Electrochemical potential difference across the cell from its equilibrium value. When the term is used in this sense, it is equivalent to “concentration overpotential”. the changes in concentration (emergence of concentration gradients in the solution adjacent to the electrode surface) is the difference