Polyacrylamide gel electrophoresis the top to create the sample wells. After the gel is polymerized the comb can be removed and the gel is ready for electrophoresis. Various buffer systems are used in PAGE depending on the nature of the sample and the experimental objective. The buffers used at the anode and cathode may be the same or different. An electric field is applied across the gel, causing the negatively charged proteins or nucleic acids to migrate across the gel away from the negative electrode (which is the cathode being that this is an electrolytic rather than galvanic cell) and towards the positive
Electrolytic capacitor are therefore smaller than aluminium electrolytic capacitors. In practice different safety margins to reach reliable components makes a comparison difficult. The anodically generated insulating oxide layer is destroyed if the polarity of the applied voltage changes. Electrolytic capacitors are based on the principle of a "plate capacitor" whose capacitance increases with larger electrode area A, higher dielectric permittivity ε, and thinner dielectric (d). The dielectric thickness of electrolytic capacitors is very small, in the range of nanometers per volt. On the other hand, the voltage strengths of these oxide layers are quite high. With this very thin dielectric oxide layer