Ultraviolet photography sensor will pick up reflected infrared radiation as well as ultraviolet, which may obscure the details that would be resolved by ultraviolet alone. Most types of glass will allow longwave UV to pass, but absorb all the other UV wavelengths, usually from about 350 nm and below. For UV photography it is necessary to use specially developed lenses having elements made from fused quartz or quartz and fluorite. Lenses based purely on quartz show a distinct focus shift between visible and UV light, whereas the fluorite/quartz lenses can be fully corrected between visible and ultraviolet light without focus shift. Examples
Ultraviolet–visible spectroscopy non-bonding electrons (n-electrons) can absorb energy in the form of ultraviolet or visible light to excite these electrons to higher anti-bonding molecular orbitals. The more easily excited the electrons (i.e. lower energy gap between the HOMO and the LUMO), the longer the wavelength of light it can absorb. There are four possible types of transitions (π–π*, n–π*, σ–σ*, and n–σ*), and they can be ordered as follows: σ–σ* > n–σ* > π–π* > n–π*. UV/Vis spectroscopy is routinely used in analytical chemistry for the quantitative determination of different analytes, such as transition metal ions, highly conjugated organic compounds, and biological