Evolution of human colour vision opsins was increasing from 400 to 430. During this time, Ancestral Boreotherian had two long wave length opsins created by gene duplication, with one retaining the absolute max value of 560, equal to the ancestral value. This led to the creation of the modern human long wavelength sensitive, or red opsin. The other short wavelength sensitive opsin decreased its absolute max value to 530 and became the middle wavelength sensitive, or green-sensitive opsin. This occurred through mutations involving S180A, Y277F and T285A. The order that these mutations took place in the ancestral Boreotheria is currently not fully known. It is
What Hurts the Most memorial on the side of the road, to kneel down and say that she saw him, answering his question asked earlier in the video "What do you see?" when they were talking about the future. The music video was directed by Shaun Silva in early 2006. Rascal Flatts' version of the song is set in the key of F minor, with a vocal range of E♭3-C 5. The main chord pattern is Fm-D♭-A♭-E♭. From "Me and My Gang" liner notes. In 2007, German Eurodance trio Cascada recorded a cover of "What Hurts the Most" for their second studio album, "Perfect