Polyacrylamide gel electrophoresis the top to create the sample wells. After the gel is polymerized the comb can be removed and the gel is ready for electrophoresis. Various buffer systems are used in PAGE depending on the nature of the sample and the experimental objective. The buffers used at the anode and cathode may be the same or different. An electric field is applied across the gel, causing the negatively charged proteins or nucleic acids to migrate across the gel away from the negative electrode (which is the cathode being that this is an electrolytic rather than galvanic cell) and towards the positive
Electrolytic detector current until the noise just ceases, at which point the detector is in its most sensitive state. It was found that strong atmospheric noise would render it insensitive, requiring that the device be rebiased after each strong burst of static interference. Another form of electrolytic detector, the sealed-point electrolytic detector, which could stand considerable rough usage, was commercially known as the Radioson Detector; it had the cell sealed in a glass envelope. The operation was the same as in the bare-point electrolytic detector, the advantage being that the acid was sealed in, and consequently could not spill or evaporate. Electrolytic