Sackbut used as a clash until the Renaissance, when it became consonant in compositions, which went hand-in-hand with the widespread use of meantone temperament. During the 17th century, Well temperament began to become more and more popular as the range of keys increased. Temperament affects the colour of a composition, and therefore modern performances, typically employing equal temperament, may not be true representations of the composers' intentions. These old tunings are the result of the natural harmonic series of a brass instrument such as the sackbut. As the bell is smaller than a modern trombone, the harmonic series is closer to
Musical instrument been used to play four notes of a diatonic scale. Researchers estimate the flute's age at between 43,400 and 67,000 years, making it the oldest known musical instrument and the only musical instrument associated with the Neanderthal culture. However, some archaeologists and ethnomusicologists dispute the flute's status as a musical instrument. German archaeologists have found mammoth bone and swan bone flutes dating back to 30,000 to 37,000 years old in the Swabian Alps. The flutes were made in the Upper Paleolithic age, and are more commonly accepted as being the oldest known musical instruments. Archaeological evidence of musical instruments was