Polyacrylamide gel electrophoresis the top to create the sample wells. After the gel is polymerized the comb can be removed and the gel is ready for electrophoresis. Various buffer systems are used in PAGE depending on the nature of the sample and the experimental objective. The buffers used at the anode and cathode may be the same or different. An electric field is applied across the gel, causing the negatively charged proteins or nucleic acids to migrate across the gel away from the negative electrode (which is the cathode being that this is an electrolytic rather than galvanic cell) and towards the positive
John (given name) masculine name in the United Kingdom, but by 2004 it had fallen out of the top 50 names for newborn boys in England and Wales. By contrast Jack, which was a nickname for John but is now established as a name in its own right, was the most popular name given to newborn boys in England and Wales every year from 1995 to 2005. However, "John" has not been a popular one for members of the royal family. The memory of King John is tainted by negative depictions of his turbulent reign and troublesome personality and by his role as