Ultraviolet–visible spectroscopy single-beam instrument, the cuvette containing only a solvent has to be measured first. Mettler Toledo developed a single beam array spectrophotometer that allows fast and accurate measurements over the UV/VIS range. The light source consists of a Xenon flash lamp for the ultraviolet (UV) as well as for the visible (VIS) and near-infrared wavelength regions covering a spectral range from 190 up to 1100 nm. The lamp flashes are focused on a glass fiber which drives the beam of light onto a cuvette containing the sample solution. The beam passes through the sample and specific wavelengths are absorbed by the
Ultraviolet short-wavelength light by killing bacteria was discovered. By 1903 it was known the most effective wavelengths were around 250 nm. In 1960, the effect of ultraviolet radiation on DNA was established. The discovery of the ultraviolet radiation with wavelengths below 200 nm, named "vacuum ultraviolet" because it is strongly absorbed by the air, was made in 1893 by the German physicist Victor Schumann. The electromagnetic spectrum of ultraviolet radiation (UVR), defined most broadly as 10–400 nanometers, can be subdivided into a number of ranges recommended by the ISO standard ISO-21348: A variety of solid-state and vacuum devices have been explored