Animal testing on non-human primates between the monkeys natural behaviors versus the behaviors expressed by the diazepam induced monkeys in thirteen different locations inside the maze. Scientist Barros and his colleagues created this model to allow the monkeys to roam a less confined environment and slightly eliminate outside factors that may induce stress. Many of the best-known allegations of abuse made by animal protection or animal rights groups against animal-testing facilities involve NHPs. The so-called "pit of despair" was used in experiments conducted on rhesus macaque monkeys during the 1970s by American comparative psychologist Harry Harlow at the University of Wisconsin–Madison.<ref name="Blum1994/95">Blum, Deborah. "The Monkey
Rhesus macaque in space and return alive. On 25 October 1999, the rhesus macaque became the first cloned primate with the birth of Tetra. January 2001 had the birth of ANDi, the first transgenic primate; ANDi carries foreign genes originally from a jellyfish. Though most studies of the rhesus macaque are from various locations in northern India, some knowledge of the natural behavior of the species comes from studies carried out on a colony established by the Caribbean Primate Research Center of the University of Puerto Rico on the island of Cayo Santiago, off Puerto Rico. No predators are on the island,