Ultraviolet–visible spectroscopy single-beam instrument, the cuvette containing only a solvent has to be measured first. Mettler Toledo developed a single beam array spectrophotometer that allows fast and accurate measurements over the UV/VIS range. The light source consists of a Xenon flash lamp for the ultraviolet (UV) as well as for the visible (VIS) and near-infrared wavelength regions covering a spectral range from 190 up to 1100 nm. The lamp flashes are focused on a glass fiber which drives the beam of light onto a cuvette containing the sample solution. The beam passes through the sample and specific wavelengths are absorbed by the
UV marker emits visible light and make the message readable for human eyes. Fluoresce is caused by a conversion of energy; when the invisible ultraviolet falls on the fluorescent surface, it is absorbed and re-emitted as visible light radiation. The absorption of energy by the electrons in the UV-visible ink enables this to happen. When an extra amount of energy is absorbed, the electrons jump into higher energy orbital patterns surrounding the nucleus. Eventually, the energy is released as visible light when they fall back to their normal orbital shell. The difference between the original electron orbital and the new orbital pattern