FinePix IS Pro filters are required to photograph in IR or UV wavelengths. Infrared filters are readily available at low cost from many sources, but ultraviolet filters – and suitable lenses – can be expensive. Photography in infrared and ultraviolet poses its own special challenges regarding exposure and – above all – focusing a SLR camera. By definition, these forms of light are invisible to the human eye. The IS Pro includes a Live View mode that relays what the sensor sees as live video to the LCD screen (and, optionally, via USB to a computer running appropriate software). Some lenses have separate
Ultraviolet–visible spectroscopy non-bonding electrons (n-electrons) can absorb energy in the form of ultraviolet or visible light to excite these electrons to higher anti-bonding molecular orbitals. The more easily excited the electrons (i.e. lower energy gap between the HOMO and the LUMO), the longer the wavelength of light it can absorb. There are four possible types of transitions (π–π*, n–π*, σ–σ*, and n–σ*), and they can be ordered as follows: σ–σ* > n–σ* > π–π* > n–π*. UV/Vis spectroscopy is routinely used in analytical chemistry for the quantitative determination of different analytes, such as transition metal ions, highly conjugated organic compounds, and biological