Legionnaires' disease showed that Legionnaires' disease was responsible and the immediate epidemiological investigation traced the source of the infection to the air-conditioning cooling tower on the roof of Stafford District Hospital. In March 1999, a large outbreak in the Netherlands occurred during the Westfriese Flora flower exhibition in Bovenkarspel; 318 people became ill and at least 32 people died. This was the second-deadliest outbreak since the 1976 outbreak and possibly the deadliest, as several people were buried before Legionnaires' disease had been diagnosed. The world's largest outbreak of Legionnaires' disease happened in July 2001 with people appearing at the hospital on July
1976 Philadelphia Legionnaires' disease outbreak testing specimens. While the Center for Disease Control responded rapidly, as did the Pennsylvania Health Department, it took six months for the CDC microbiologist Joseph McDade to discover the cause of the outbreak. He initially thought the cause was viral because blood and tissues from the victims failed to grow bacteria when incubated in culture medium. His subsequent attempts to isolate the viral agent in eggs included antibiotics to kill off "contaminating" bacteria. It was only when he omitted the antibiotics that the bacteria grew in eggs. Attempts to reproduce the disease in mice, the standard laboratory animal model, also