Bald-faced hornet hydrocarbons serve as a barrier to moisture diffusion and thus prevent dehydration in wasps. Cuticular hydrocarbon profiles vary over species and nests, and so cuticular hydrocarbons are a proposed mechanism for nest-mate recognition. Worker and queen cuticular lipids have similar components, but their distributions differ dramatically, implying that cuticular hydrocarbons also play a role in caste differentiation. The following lipid profiles were determined from specimens collected in the summer and early fall of northeastern Georgia in 1989. The dominant hydrocarbons recovered from the cuticles of workers were n-alkanes and methyl branched and dimethyl branched alkanes with a C27 or a
Bald-faced hornet C29 backbone. The major lipids and their distributions in workers were as follows: n-heptacosane(28%), 11-13-methylheptacosane (15%) 3,13-dimethylheptacosane (11%) and 13 and 15-methylnonacosane (10%). Nonacosene comprised 34% of cuticular lipids in the queen. The average chain length of identified cuticular lipids is 28.0 in queens and 27.5 in workers. In "D. maculata", queens mate with only one male which results in a worker relatedness of 0.75, whereas the relatedness between the queen and worker is only 0.5. Gynes in "D. maculata" have the ability to discriminate between fragments of natal comb and foreign comb. Recognition does not depend upon presence of