Asplenium rhizophyllum one collection on sandstone in the Shawnee Hills of Illinois. A triploid hybrid between walking fern and Tutwiler's spleenwort was accidentally produced in culture. A similar plant collected from limestone in Shepherdstown, West Virginia could have originated from the same parents, from an unreduced (diploid) gametophyte of Scott's spleenwort crossed with walking fern, or from an unreduced walking fern gametophyte crossed with ebony spleenwort. In 1813, Henry Muhlenberg listed lobed spleenwort as "Asplenium rhizophyllum" var. "pinnatifidum", although he did not provide a description distinguishing the variety from the typical species. It was described as the species "A. pinnatifidum" by Thomas
Asplenium platyneuron result of long-distance dispersal, which may also have allowed it to colonize and naturalize in South Africa. While globally secure, ebony spleenwort is considered an endangered species in some of the states and provinces at the northern and western edges of its North American range. NatureServe considers it to be critically imperiled in Arizona and Colorado, imperiled in Nebraska, Maine, Rhode Island, and Quebec, and vulnerable in Minnesota. The ebony spleenwort is sometimes grown as a terrarium or garden plant. It can be cultivated in sandy peat, subacid garden soil, other gravelly, sandy or gritty soils, or potting mix under