Bald-faced hornet viable brood in the comb fragment. The physical nature of cues mediating natal comb recognition is unknown, though some researchers propose distinct cuticular hydrocarbon profiles allow wasps to recognize nest mates. "D. maculata" is characterized by low paternity, worker reproduction and queen-worker conflict. Divergent genetic interests between workers and their queen cause intra-nest struggle for control, which disrupts social organization. Because of haplodiploidy, workers are unable to mate. However, their unfertilized eggs become males. Workers and their queens are most related to their own sons. Natural selection will therefore favor those workers who produce their own sons rather than rearing
Bald-faced hornet acts as a selective pressure on the workers, so they are more likely to exercise reproductive restraint. It has been suggested that workers in reproductive nests may kill their queen so they can reproduce. When researchers at the University of Sheffield examined a collection of nineteen "D. maculata" nests during the reproductive phase of their life cycle, they found that 14/19 nests did not have a queen. Matricide might occur after sufficient workers have been raised and queen-destined eggs have been laid. However, matricide has not been directly observed and other causes of death are possible. Diet in "D. maculata"