Bald-faced hornet from mid-April to early October, and reproductives were produced from mid-July through the end of November. Onset of male output usually precedes that of queen output in social wasps, however here "D. maculata" is an exception to this trend. Energy investment by workers required to produce reproductives is considerable. Newly emerged males and queens are no more functional than pupae; both depend heavily on solid prey brought in by workers, thus competing with larvae for food resources. As a result, worker quantity must remain high in order to maintain reproductive output. Workers must maintain food stores and defend the nest,
Bald-faced hornet C29 backbone. The major lipids and their distributions in workers were as follows: n-heptacosane(28%), 11-13-methylheptacosane (15%) 3,13-dimethylheptacosane (11%) and 13 and 15-methylnonacosane (10%). Nonacosene comprised 34% of cuticular lipids in the queen. The average chain length of identified cuticular lipids is 28.0 in queens and 27.5 in workers. In "D. maculata", queens mate with only one male which results in a worker relatedness of 0.75, whereas the relatedness between the queen and worker is only 0.5. Gynes in "D. maculata" have the ability to discriminate between fragments of natal comb and foreign comb. Recognition does not depend upon presence of