Discovery and development of direct thrombin inhibitors the binding site for the globular amino-terminal domain and exosite 1 is the binding site for the acidic carboxy-terminal domain of hirudin. Native hirudin, a 65-amino-acid polypeptide, is produced in the parapharyngeal glands of medicinal leeches. Hirudins today are produced by recombinant biotechnology using yeast. These recombinant hirudins lack a sulfate group at Tyr-63 and are therefore called desulfatohirudins. They have a 10-fold lower binding affinity to thrombin compared to native hirudin, but remain a highly specific inhibitor of thrombin and have an inhibition constant for thrombin in the picomolar range. Renal clearance and degradation account for the most part
The Glands with Yo La Tengo. Ross Shapiro, singer, guitarist, and founding member of The Glands, died in March 2016 following a battle with an illness. The Glands The Glands were an American indie rock band from Athens, Georgia, United States. Their first CD, "Double Thriller", was self-released in 1996 and reissued on Bar/None Records in 1998. Their self-titled album was first released in 2000 on Capricorn Records and reissued on Velocette Records in August 2001. The Glands' unique sounds recalled a variety of influences, including the Kinks, the Who, and The Rolling Stones. The song "Livin' Was Easy" was featured in