Discovery and development of direct thrombin inhibitors the binding site for the globular amino-terminal domain and exosite 1 is the binding site for the acidic carboxy-terminal domain of hirudin. Native hirudin, a 65-amino-acid polypeptide, is produced in the parapharyngeal glands of medicinal leeches. Hirudins today are produced by recombinant biotechnology using yeast. These recombinant hirudins lack a sulfate group at Tyr-63 and are therefore called desulfatohirudins. They have a 10-fold lower binding affinity to thrombin compared to native hirudin, but remain a highly specific inhibitor of thrombin and have an inhibition constant for thrombin in the picomolar range. Renal clearance and degradation account for the most part
Salivary diagnostics Salivary diagnostics Salivary diagnostics or Salivaomics is a procedure for testing and maintaining the data for diagnostic purposes. Saliva based hand-held sensors are used to detect and measure proteins and nucleic acids that are present in saliva for diagnosis. Salivary diagnostic technology is showing promise in the detection and monitoring malignancies at early stage and other various health concerns. The advantages of salivary diagnostics includes ease of collection, elimination of fear of needles, inexpensive testing and reduce risk of disease transmission between healthcare workers and patients. In addition, salivary diagnostic is fast, highly sensitive and specific, portable, user-friendly and can