Felidae carnivores, meaning they are dependent on nutrients in animal flesh for survival, and because of the large proportion of meat in their diet they are sometimes referred to as hypercarnivores. Of the 13 terrestrial families in the order Carnivora, they are the strictest carnivores. The Felidae comprises two subfamilies, the Pantherinae and the Felinae. The former includes the "Panthera" species tiger, lion, jaguar, leopard, snow leopard, and "Neofelis" species clouded leopard and Sunda clouded leopard. All the non-pantherine cats are part of the Felinae, which includes several genera and the majority of cat species. The first cats emerged during the
Animals (Scientific Procedures) Act 1986 be done through non-animal or in-vitro methods. The project must also pass an ethical review panel which aims to decide if the potential benefits outweigh any suffering for the animals involved. ASPA involves three levels of regulation — person, project, and place. The 'person' level is achieved by the granting of a "personal licence" (PIL, procedure personal licence) to a researcher wishing to carry out regulated procedures on a protected animal. Having undergone a defined sequence of training, a researcher can apply for a PIL permitting specified techniques to be carried out on named species of animals. The 'project' level