Jana Jonášová with Bendlová and then at the Academy of Performing Arts in Prague with Teodor Šrubař. She made her professional opera debut in 1965 at the Liberec Theatre as Konstanze in Wolfgang Amadeus Mozart's "The Abduction from the Seraglio". That same year she became involved with the Chamber Opera Prague. She remained committed to the Liberec Theatre through 1970 and then joined the National Theatre in Prague where she initially sang minor roles and worked in the opera chorus. In 1973 Jonášová was promoted to principal sopranos at the National Theatre and she quickly became one of the house's most important
Lisette Oropesa Cleveland; the St Matthew Passion in Chicago; "Abduction from the Seraglio" in Bellingham, Washington; and a reprise of her 2010 performance in "Le Nozze di Figaro" at Ravinia Festival. Opera engagements included Nannetta at the Dutch National Opera; Gilda at Grand Théâtre de Genève; and Konstanze at the Bavarian State Opera. Her last performance of 2014 was her debut at the Los Angeles Opera as Rosalba in Daniel Catán's "Florencia en el Amazonas". In 2015, she started the year at the Paris Opera as Konstanze with success "Oropesa, seguita da Marten aller Arten, cantata con accenti incisivi e appassionata recitazione."