Little Jack Horner in the Tudor period. The song’s most common lyrics are: <poem style="margin-left:24px> Little Jack Horner Sat in the corner, Eating his Christmas pie; He put in his thumb, And pulled out a plum, And said, "What a good boy am I!"</poem> It was first documented in full in the nursery rhyme collection "Mother Goose's melody, or, Sonnets for the cradle", which may date from 1765, although the earliest surviving English edition is from 1791. The melody commonly associated with the rhyme was first recorded by the composer and nursery rhyme collector James William Elliott in his "National Nursery Rhymes and
Ponceau S easily reversed with water washes, facilitating subsequent immunological detection. The stain can be completely removed from the protein bands by continued washing. Common stain formulations include 0.1% (w/v) Ponceau S in 5% acetic acid or 2% (w/v) Ponceau S in 30% trichloroacetic acid and 30% sulfosalicylic acid. Ponceau S Ponceau S, Acid Red 112, or C.I. 27195 (systematic name: 3-hydroxy-4-(2-sulfo-4-[4-sulfophenylazo]phenylazo)-2,7-naphthalenedisulfonic acid sodium salt) is a sodium salt of a diazo dye of a light red color, that may be used to prepare a stain for rapid reversible detection of protein bands on nitrocellulose or polyvinylidene fluoride (PVDF) membranes (Western blotting),