Jana Jonášová with Bendlová and then at the Academy of Performing Arts in Prague with Teodor Šrubař. She made her professional opera debut in 1965 at the Liberec Theatre as Konstanze in Wolfgang Amadeus Mozart's "The Abduction from the Seraglio". That same year she became involved with the Chamber Opera Prague. She remained committed to the Liberec Theatre through 1970 and then joined the National Theatre in Prague where she initially sang minor roles and worked in the opera chorus. In 1973 Jonášová was promoted to principal sopranos at the National Theatre and she quickly became one of the house's most important
Lisette Oropesa at the Ravinia Festival as Susanna in "Le nozze di Figaro", conducted by James Conlon; for Opera New Jersey as Lucia in "Lucia di Lammermoor"; at Arizona Opera as Gilda in "Rigoletto", again as Lucia; and at the New Orleans Opera as Gilda and as Leïla in "Les pêcheurs de perles". In April and May 2012, Oropesa appeared with the Pittsburgh Opera as Konstanze in a production of "The Abduction from the Seraglio" set on the Orient Express in the Pasha's private train car. In October 2012, she again performed the role of Lucia with the Arizona Opera to success.