Jack and Jill (nursery rhyme) and brown paper were a home cure used as a method to draw out bruises on the body. Jack is the most common name used in English-language nursery rhymes and represented an archetypal Everyman hero by the 18th century, while Jill or Gill had come to mean a young girl or a sweetheart by the end of the Middle Ages. However, the woodcut that accompanied the first recorded version of the rhyme showed two boys (not a boy and a girl) and used the spelling Gill not Jill. The true origin of the rhyme is unknown, but there are several
Ponceau S easily reversed with water washes, facilitating subsequent immunological detection. The stain can be completely removed from the protein bands by continued washing. Common stain formulations include 0.1% (w/v) Ponceau S in 5% acetic acid or 2% (w/v) Ponceau S in 30% trichloroacetic acid and 30% sulfosalicylic acid. Ponceau S Ponceau S, Acid Red 112, or C.I. 27195 (systematic name: 3-hydroxy-4-(2-sulfo-4-[4-sulfophenylazo]phenylazo)-2,7-naphthalenedisulfonic acid sodium salt) is a sodium salt of a diazo dye of a light red color, that may be used to prepare a stain for rapid reversible detection of protein bands on nitrocellulose or polyvinylidene fluoride (PVDF) membranes (Western blotting),