Jack and Jill (nursery rhyme) these stanzas were viewed as quatrains with internal rhymes, this would be an example of ballad form, a common form for nursery rhymes. The melody commonly associated with the rhyme was first recorded by the composer and nursery rhyme collector James William Elliott in his "National Nursery Rhymes and Nursery Songs" (1870). The Roud Folk Song Index, which catalogues folk songs and their variations by number, classifies the song as 10266. The phrase "Jack and Jill" was in use in England as early as the 16th century to indicate a boy and a girl. A comedy with the title "Jack
Ponceau S easily reversed with water washes, facilitating subsequent immunological detection. The stain can be completely removed from the protein bands by continued washing. Common stain formulations include 0.1% (w/v) Ponceau S in 5% acetic acid or 2% (w/v) Ponceau S in 30% trichloroacetic acid and 30% sulfosalicylic acid. Ponceau S Ponceau S, Acid Red 112, or C.I. 27195 (systematic name: 3-hydroxy-4-(2-sulfo-4-[4-sulfophenylazo]phenylazo)-2,7-naphthalenedisulfonic acid sodium salt) is a sodium salt of a diazo dye of a light red color, that may be used to prepare a stain for rapid reversible detection of protein bands on nitrocellulose or polyvinylidene fluoride (PVDF) membranes (Western blotting),