Nursery rhyme rhymes became associated with the idea of political correctness. Most attempts to reform nursery rhymes on this basis appear to be either very small scale, light-hearted updating, like Felix Dennis's "When Jack Sued Jill – Nursery Rhymes for Modern Times" (2006), or satires written as if from the point of view of political correctness in order to condemn reform. The controversy in Britain in 1986 over changing the language of "Baa Baa Black Sheep" because, it was alleged in the popular press, it was seen as racially dubious, was apparently based only on a rewriting of the rhyme in one
Ponceau S easily reversed with water washes, facilitating subsequent immunological detection. The stain can be completely removed from the protein bands by continued washing. Common stain formulations include 0.1% (w/v) Ponceau S in 5% acetic acid or 2% (w/v) Ponceau S in 30% trichloroacetic acid and 30% sulfosalicylic acid. Ponceau S Ponceau S, Acid Red 112, or C.I. 27195 (systematic name: 3-hydroxy-4-(2-sulfo-4-[4-sulfophenylazo]phenylazo)-2,7-naphthalenedisulfonic acid sodium salt) is a sodium salt of a diazo dye of a light red color, that may be used to prepare a stain for rapid reversible detection of protein bands on nitrocellulose or polyvinylidene fluoride (PVDF) membranes (Western blotting),