Felidae carnivores, meaning they are dependent on nutrients in animal flesh for survival, and because of the large proportion of meat in their diet they are sometimes referred to as hypercarnivores. Of the 13 terrestrial families in the order Carnivora, they are the strictest carnivores. The Felidae comprises two subfamilies, the Pantherinae and the Felinae. The former includes the "Panthera" species tiger, lion, jaguar, leopard, snow leopard, and "Neofelis" species clouded leopard and Sunda clouded leopard. All the non-pantherine cats are part of the Felinae, which includes several genera and the majority of cat species. The first cats emerged during the
Animals (Scientific Procedures) Act 1986 additional protection over other vertebrates under the Act. Revised legislation came into force on January 1, 2013. The Act has been expanded to protect - The definition of regulated procedures was expanded to - ASPA also regulates the modification of genes in protected animals if this causes the animal pain, suffering, distress or lasting harm. Other considerations in the Act include animal sources, housing conditions, identification methods and the humane killing of animals. This legislation is widely regarded as the strictest in the world protecting animals used in research. Those applying for a license must explain why such research cannot