Harvest (wine) of wine, winemakers would use the sugar and acid levels of the grape as a guide in determining ripeness. Early winemakers tasted the grapes to gauge ripeness. Modern winemakers use a refractometer to measure high sugar levels and °Brix or titration tests (using an indicator such as phenolphthalein) to determine the titratable acidity within the grape. In recent times there has been more of an emphasis on the "physiological" ripeness of the grape, usually in the form of tannins and other phenolics. Currently, tasting is the only way to measure tannin ripeness, which can take experience and skill to do
Phenolic content in wine into wine, most notably vanillin which adds vanilla aroma to wines. Most wine phenols are classified as secondary metabolites and were not thought to be active in the primary metabolism and function of the grapevine. However, there is evidence that in some plants flavonoids play a role as endogenous regulators of auxin transport. They are water-soluble and are usually secreted into the vacuole of the grapevine as glycosides. "Vitis vinifera" produces many phenolic compounds. There is a varietal effect on the relative composition. In red wine, up to 90% of the wine's phenolic content falls under the classification of flavonoids.