Acids in wine ripened grapes. While concentration varies among grape varieties, it is usually found in higher levels with red wine grapes. The acid is created as a byproduct of the metabolization of nitrogen by yeast cells during fermentation. The combination of succinic acid with one molecule of ethanol will create the ester mono-ethyl succinate responsible for a mild, fruit aroma in wines. Acidity is highest in wine grapes just before the start of "veraison", which ushers in the ripening period of the annual cycle of grape vines. As the grapes ripen, their sugar levels increase and their acidity levels decrease. Through the
Brix prism; the result is observed through an eyepiece. The critical angle (the angle beyond which light is totally reflected back into the sample) is a function of the refractive index and the operator detects this critical angle by noting where a dark-bright boundary falls on an engraved scale. The scale can be calibrated in Brix or refractive index. Often the prism mount contains a thermometer which can be used to correct to 20 °C in situations where measurement cannot be made at exactly that temperature. These instruments are available in bench and handheld versions. Digital refractometers also find the critical