Suicide in literature a crime of passion in "Othello". The play "Antony and Cleopatra" ends with five suicides, including the deaths of both Antony and Cleopatra. Ophelia commits suicide in "Hamlet" following the death of her father. The subject of suicide itself is controversial. While the act of suicide can be symbolic in literature, the act itself still possesses the ability to cause controversy in the real world. Kate Chopin's novel, "The Awakening", was extremely controversial when it was released in 1899. Some authors who have created characters that commit suicide have committed suicide themselves. Ernest Hemingway shot himself in 1961; Some of
Shotgun proteomics Shotgun proteomics Shotgun proteomics refers to the use of bottom-up proteomics techniques in identifying proteins in complex mixtures using a combination of high performance liquid chromatography combined with mass spectrometry. The name is derived from shotgun sequencing of DNA which is itself named after the rapidly expanding, quasi-random firing pattern of a shotgun. The most common method of shotgun proteomics starts with the proteins in the mixture being digested and the resulting peptides are separated by liquid chromatography. Tandem mass spectrometry is then used to identify the peptides. Targeted proteomics using SRM and data-independent acquisition methods are often considered alternatives