Brazilian gracile opossum habitats than more purely arboreal animals. It has also been found in artificial plantations. There are no recognised subspecies. Brazilian gracile opossums are arboreal and nocturnal, spending the day nesting in tree hollows. They are solitary animals, with each individual inhabiting a home range of anything from , depending on habitat. Males tend to have larger home ranges than females, presumably because, being larger, they require more food. The species is insectivorous, and is an opportunistic forager, not specialising in any particular kind of insect. Individuals have also been reported to eat some spiders, snails, and even fruit (including passionfruit).
Arboreal locomotion its descent and prevent falling. Descent can be particularly problematic for many animals, and highly arboreal species often have specialized methods for controlling their descent. Due to the height of many branches and the potentially disastrous consequences of a fall, balance is of primary importance to arboreal animals. On horizontal and gently sloped branches, the primary problem is tipping to the side due to the narrow base of support. The narrower the branch, the greater the difficulty in balancing a given animal faces. On steep and vertical branches, tipping becomes less of an issue, and pitching backwards or slipping downwards