Brazilian gracile opossum habitats than more purely arboreal animals. It has also been found in artificial plantations. There are no recognised subspecies. Brazilian gracile opossums are arboreal and nocturnal, spending the day nesting in tree hollows. They are solitary animals, with each individual inhabiting a home range of anything from , depending on habitat. Males tend to have larger home ranges than females, presumably because, being larger, they require more food. The species is insectivorous, and is an opportunistic forager, not specialising in any particular kind of insect. Individuals have also been reported to eat some spiders, snails, and even fruit (including passionfruit).
Arboreal locomotion passive stability by hanging beneath the branch. Both pitching and tipping become irrelevant, as the only method of failure would be losing their grip. Arboreal species have behaviors specialized for moving in their habitats, most prominently in terms of posture and gait. Specifically, arboreal mammals take longer steps, extend their limbs further forwards and backwards during a step, adopt a more 'crouched' posture to lower their center of mass, and use a diagonal sequence gait. Arboreal locomotion allows animals access to different resources, depending upon their abilities. Larger species may be restricted to larger-diameter branches that can support their weight,