Pollen tube subsequent pollination (transfer of pollen grains to the pistil, the female reproductive organ). Transfer of pollen is usually carried out by wind, water, or animals . The ovaries hold the ovules that produce the female gamete: the egg cell, which waits in place for fertilization. Once a pollen grain settles on a compatible pistil, it may germinate in response to a sugary fluid secreted by the mature stigma. Lipids at the surface of the stigma may also stimulate pollen tube growth for compatible pollen. Plants that are self-sterile often inhibit the pollen grains from their own flowers from growing pollen
Spin coating to 80 revolutions per second for 30 to 60 seconds. Spin coating Spin coating is a procedure used to deposit uniform thin films to flat substrates. Usually a small amount of coating material is applied on the center of the substrate, which is either spinning at low speed or not spinning at all. The substrate is then rotated at high speed in order to spread the coating material by centrifugal force. A machine used for spin coating is called a spin coater, or simply spinner. Rotation is continued while the fluid spins off the edges of the substrate, until the