Pollen tube by recognition and hydration of the pollen that allows for germination of the tube and ultimate growth. In the pollen grain, the generative cell gives rise to the sperm, whereas the vegetative cells have a tube cell that grows the pollen tube. There is competition in this step as many pollen grains may compete to reach the egg. The stigma plays a role in guiding the sperm to a receptive ovule, in the case of many ovules. Only compatible pollen grains are allowed to grow as determined by signaling with the stigma. Some plants have mechanisms in place to prevent
Spin coating to 80 revolutions per second for 30 to 60 seconds. Spin coating Spin coating is a procedure used to deposit uniform thin films to flat substrates. Usually a small amount of coating material is applied on the center of the substrate, which is either spinning at low speed or not spinning at all. The substrate is then rotated at high speed in order to spread the coating material by centrifugal force. A machine used for spin coating is called a spin coater, or simply spinner. Rotation is continued while the fluid spins off the edges of the substrate, until the