Pollen tube are tolerant and even pollen damaged by X-rays and gamma rays can still grow pollen tubes. The interaction between the stigma-style and the pollen grain is a vital piece that contributes to the growth. The elongation of the tube is achieved with elongation of the cytoskeleton and it extends from the tip, which is regulated by high levels of calcium in the cytosol. The calcium levels help the synaptic vesicles in the membranes grow and extend at the tip. Polypeptides found in the style also regulate growth of tube and specific peptides that play a role in signaling for growth
Spin coating Spin coating Spin coating is a procedure used to deposit uniform thin films to flat substrates. Usually a small amount of coating material is applied on the center of the substrate, which is either spinning at low speed or not spinning at all. The substrate is then rotated at high speed in order to spread the coating material by centrifugal force. A machine used for spin coating is called a spin coater, or simply spinner. Rotation is continued while the fluid spins off the edges of the substrate, until the desired thickness of the film is achieved. The applied solvent