Pollen tube the pollen tube responds to extracellular guidance signals to achieve fertilization. Pollen tubes are produced by the male gametophytes of seed plants. They act as conduits to transport the male gamete cells from the pollen grain—either from the stigma (in flowering plants) to the ovules at the base of the pistil or directly through ovule tissue in some gymnosperms. Pollen tubes were first discovered by Giovanni Battista Amici. Pollen tube formation is important for sexual reproduction to occur in seed plants. Plants have separate structures such as microsporocytes and megasporocytes and the pollen grains are transported to the female gametophyte
Spin coating Spin coating Spin coating is a procedure used to deposit uniform thin films to flat substrates. Usually a small amount of coating material is applied on the center of the substrate, which is either spinning at low speed or not spinning at all. The substrate is then rotated at high speed in order to spread the coating material by centrifugal force. A machine used for spin coating is called a spin coater, or simply spinner. Rotation is continued while the fluid spins off the edges of the substrate, until the desired thickness of the film is achieved. The applied solvent