Pollen tube are tolerant and even pollen damaged by X-rays and gamma rays can still grow pollen tubes. The interaction between the stigma-style and the pollen grain is a vital piece that contributes to the growth. The elongation of the tube is achieved with elongation of the cytoskeleton and it extends from the tip, which is regulated by high levels of calcium in the cytosol. The calcium levels help the synaptic vesicles in the membranes grow and extend at the tip. Polypeptides found in the style also regulate growth of tube and specific peptides that play a role in signaling for growth
Spore print print is pure white or, rather, very slightly pigmented. The mushroom is left for several hours, often overnight, in this manner. Some guides advise using a moisture-resistant enclosure, like a glass or jar, to contain the mushroom during printing. If the mushroom is to be preserved, a small hole can be made in the spore print paper rather than cutting the stipe. When the mushroom is removed, the color of the spores should be visible. Mycologists often use glass slides, which allow for quick examination of spores under a microscope. A mushroom cannot be identified from its spore print alone;