Campolina Holsteiner, and American Saddle Horse. Bloodlines from the Mangalarga Marchador, were also added to refine the Campolina. The herdbook was closed in 1934 and the breed standard first defined. In 1938, the Professional Consortium of Campolina Horse Breeders was formed to formally organize the breed, and in 1951 the organization was renamed to the Campolina Breeders Association, the breed standards were formally adopted, with the organization based in Belo Horizonte. There were further updates to the breed standard in 1975 and 1993. There are currently around 85,000 registered Campolina horses, with slightly over 7,300 registered breeders. Around 4,300 mares were
Cryoconservation of animal genetic resources area, making the process of collecting germplasms easier and more efficient. The species and breeds of animals housed should be considered while planning the facility; facilities should be big enough to meet animal welfare standards, yet small enough to reduce human contact and increase ease of handling while reducing stress of the animal. As the process of collecting germplasm may take several days, the animal may become stressed causing a lower quality of genetic material to be obtained. Thus, training the animal to become familiar with the process is key. Holding facilities for animals may also serve as a quarantine.