Robert Jarvik animal in the lab for 10 days. Kolff assigned Jarvik to design a new heart that would overcome the problems of the Kwan-Gett heart, eventually culminating with the Jarvik-7 device. In 1982, the team carried out an artificial heart implant - the second ever, 13 years after Domingo Liotta and Denton Cooley's first in 1969. William DeVries first implanted the Jarvik-7 into retired dentist Barney Clark at the University of Utah on December 2, 1982. Clark required frequent visits to the hospital for the next 112 days, after which he died. During frequent press conferences to update the patient's condition,
Human artificial chromosome artificial chromosome vectors. The first is to create a small minichromosome by altering a natural human chromosome. This is accomplished by truncating the natural chromosome, followed by the introduction of unique genetic material via the Cre-Lox system of recombination. The second method involves the literal creation of a novel chromosome "de novo". Progress regarding "de novo" HAC formation has been limited, as many large genomic fragments will not successfully integrate into "de novo" vectors. Another factor limiting "de novo" vector formation is limited knowledge of what elements are required for construction, specifically centromeric sequences. A 2009 study has shown additional