Percutaneous umbilical cord blood sampling prepared with an anticoagulant, which helps to reduce the risk of clot formation. During the procedure, the first step is to locate a relatively stable segment of the umbilical cord. A typical sampling site would be where the segment of the umbilical cord is closest to the placenta. However, there is a risk of maternal blood contamination at this site. Blood sampling may be achieved with more ease if the placenta is in the anterior position. However, if the placenta is in the posterior position, the fetus might block direct access to the umbilical cord. Once the umbilical cord is
Cord blood method most commonly used in clinical practice is the "closed technique", which is similar to standard blood collection techniques. With this method, the technician cannulates the vein of the severed umbilical cord using a needle that is connected to a blood bag, and cord blood flows through the needle into the bag. On average, the closed technique enables collection of about 75 ml of cord blood. Collected cord blood is cryopreserved and then stored in a cord blood bank for future transplantation. Cord blood collection is typically depleted of red blood cells before cryopreservation to ensure high rates of stem