Science and technology in Argentina Argentina have enjoyed an international respect since the turn of the 1900s, when Dr. Luis Agote devised the first safe and effective means of blood transfusion as well as René Favaloro, who was a pioneer in the improvement of the bypass surgery . Argentina is the Latin American country with the most Nobel Prize laureates; and has three Nobel Prize winners in the sciences: Bernardo Houssay in Physiology or Medicine in 1947, Luis Federico Leloir in Chemistry in 1970, and César Milstein in Physiology or Medicine in 1984. Dr. Domingo Liotta created the first artificial heart in 1969, revolutionizing the
Human artificial chromosome artificial chromosome vectors. The first is to create a small minichromosome by altering a natural human chromosome. This is accomplished by truncating the natural chromosome, followed by the introduction of unique genetic material via the Cre-Lox system of recombination. The second method involves the literal creation of a novel chromosome "de novo". Progress regarding "de novo" HAC formation has been limited, as many large genomic fragments will not successfully integrate into "de novo" vectors. Another factor limiting "de novo" vector formation is limited knowledge of what elements are required for construction, specifically centromeric sequences. A 2009 study has shown additional