Isomeric shift levels E, followed by emission of photons. Atomic levels are a manifestation of the electromagnetic interaction between electrons and nuclei. The energy levels of two atoms the nuclei of which are different isotopes of the same element are shifted one with respect to the other, despite the fact that the electric charges Z of the two isotopes are identical. This is so because isotopes differ by the number of neutrons and therefore the masses and volumes of two isotopes are different; these differences give rise to the isotopic shift on atomic spectral lines. In the case of two nuclear isomers
Nuclear physics as a function of atomic and neutron numbers, the binding energy of the nuclides forms what is known as the valley of stability. Stable nuclides lie along the bottom of this energy valley, while increasingly unstable nuclides lie up the valley walls, that is, have weaker binding energy. The most stable nuclei fall within certain ranges or balances of composition of neutrons and protons: too few or too many neutrons (in relation to the number of protons) will cause it to decay. For example, in beta decay a nitrogen-16 atom (7 protons, 9 neutrons) is converted to an oxygen-16 atom