Domingo Liotta shortly thereafter. 1962 (May): Prolonged assisted circulation during and after cardiac and aortic surgery. Prolonged partial left ventricular bypass by means of intracorporeal circulation. It was selected as Finalist for the Young Investigators´ Contest Award (Denver) of the American College of Cardiology. 1964: Annual Award, Southwestern Surgical Society, USA. Shared with C. William Hall and Michael E. DeBakey.1968: Golden Eagle Award, Council of International Nontheatrical Events, USA, shared with Denton A. Cooley, Robert Bloodwell, and Grady Hallman. 1969: Award of Merit, American Medical Association shared with Denton A. Cooley, MD. 1969: Decoration of the Institute of Spanish Culture, Madrid, Spain.
Human artificial chromosome by these vectors not only leads to different expression levels, but the inserts also disrupt the original genome. HACs differ in this regard, as they are entirely separate chromosomes. This separation from existing genetic material assumes that no insertional mutants would arise. This stability and accuracy makes HACs preferable to other methods such as viral vectors, YACs and BACs. HACs allow for delivery of more DNA (including promoters and copy-number variation) than is possible with viral vectors. Yeast artificial chromosomes and bacterial artificial chromosomes were created before human artificial chromosomes, which first appeared in 1997. HACs are useful in expression