Domingo Liotta Domingo Liotta Domingo Santo Liotta (born November 29, 1924) is a pioneer of heart surgery, creator of multiple cardiac prostheses including the first total artificial heart used in a human being. Domingo Santo Liotta son of Italian immigrants, was born in the city of Diamante, Entre Rios, Argentina on November 29, 1924. He completed his elementary school at his hometown at "Independencia School", and his high school at the "Justo Jose de Urquiza School" in Concepcion del Uruguay, Entre Rios. In 1949, he graduated as a Medical Doctor at National University of Córdoba (Argentina). He received a doctorate in Medicine
Human artificial chromosome artificial chromosome vectors. The first is to create a small minichromosome by altering a natural human chromosome. This is accomplished by truncating the natural chromosome, followed by the introduction of unique genetic material via the Cre-Lox system of recombination. The second method involves the literal creation of a novel chromosome "de novo". Progress regarding "de novo" HAC formation has been limited, as many large genomic fragments will not successfully integrate into "de novo" vectors. Another factor limiting "de novo" vector formation is limited knowledge of what elements are required for construction, specifically centromeric sequences. A 2009 study has shown additional