Domingo Liotta Domingo Liotta Domingo Santo Liotta (born November 29, 1924) is a pioneer of heart surgery, creator of multiple cardiac prostheses including the first total artificial heart used in a human being. Domingo Santo Liotta son of Italian immigrants, was born in the city of Diamante, Entre Rios, Argentina on November 29, 1924. He completed his elementary school at his hometown at "Independencia School", and his high school at the "Justo Jose de Urquiza School" in Concepcion del Uruguay, Entre Rios. In 1949, he graduated as a Medical Doctor at National University of Córdoba (Argentina). He received a doctorate in Medicine
Human artificial chromosome by these vectors not only leads to different expression levels, but the inserts also disrupt the original genome. HACs differ in this regard, as they are entirely separate chromosomes. This separation from existing genetic material assumes that no insertional mutants would arise. This stability and accuracy makes HACs preferable to other methods such as viral vectors, YACs and BACs. HACs allow for delivery of more DNA (including promoters and copy-number variation) than is possible with viral vectors. Yeast artificial chromosomes and bacterial artificial chromosomes were created before human artificial chromosomes, which first appeared in 1997. HACs are useful in expression