Domingo Liotta and Surgery in 1953. In 1955, he developed a technique for early diagnosis of tumors in the pancreas and ampulla of Vater (Pour le diagnostic des tumeurs du pancreas: La duodenographie hypotonique. Lyon Chirurgical, 1955). Liotta continued his medical career at the University of Lyon (France) at the "Pierre Mallet-Guy's" General Surgery Service. Then, he trained in thoracic and cardiac surgery, with Professors Paul Santy and Pierre Marion in Lyon France. His early works in the artificial heart are from 1958 in Córdoba, Argentina, where he developed an early prototype successfully used in small animals. After publishing the results of
Human artificial chromosome artificial chromosome vectors. The first is to create a small minichromosome by altering a natural human chromosome. This is accomplished by truncating the natural chromosome, followed by the introduction of unique genetic material via the Cre-Lox system of recombination. The second method involves the literal creation of a novel chromosome "de novo". Progress regarding "de novo" HAC formation has been limited, as many large genomic fragments will not successfully integrate into "de novo" vectors. Another factor limiting "de novo" vector formation is limited knowledge of what elements are required for construction, specifically centromeric sequences. A 2009 study has shown additional