Nitrate test to 2.5 micrograms and a concentration of 1 in 25,000 parts. Devarda's alloy (Copper/Aluminium/Zinc) is a reducing agent. When reacted with nitrate in sodium hydroxide solution, ammonia is liberated. The ammonia formed may be detected by its characteristic odor, and by damp red litmus paper's turning blue, signalling that it is an alkali — very few gases other than ammonia evolved from wet chemistry are alkaline. Aluminium is the reducing agent in this reaction that will occur. Diphenylamine may be used as a wet chemical test for the presence of the nitrate ion. In this test, a solution of diphenylamine
Acidity function is uncommon. Hammett-type acidity functions are defined in terms of a buffered medium containing a weak base B and its conjugate acid BH: where p"K" is the dissociation constant of BH. They were originally measured by using nitroanilines as weak bases or acid-base indicators and by measuring the concentrations of the protonated and unprotonated forms with UV-visible spectroscopy. Other spectroscopic methods, such as NMR, may also be used. The function "H" is defined similarly for strong bases: Here BH is a weak acid used as an acid-base indicator, and B is its conjugate base. In dilute aqueous solution, the predominant