Nitrate test to 2.5 micrograms and a concentration of 1 in 25,000 parts. Devarda's alloy (Copper/Aluminium/Zinc) is a reducing agent. When reacted with nitrate in sodium hydroxide solution, ammonia is liberated. The ammonia formed may be detected by its characteristic odor, and by damp red litmus paper's turning blue, signalling that it is an alkali — very few gases other than ammonia evolved from wet chemistry are alkaline. Aluminium is the reducing agent in this reaction that will occur. Diphenylamine may be used as a wet chemical test for the presence of the nitrate ion. In this test, a solution of diphenylamine
Heberly Run load is . The acidity concentration at the headwaters of Heberly Run is 4.40 milligrams per liter and the alkalinity concentration is 5.84 milligrams per liter. The daily loads of these substances are and , respectively. Downstream of Meeker Run, the acidity concentration is 2.87 milligrams per liter and the acidity load is per day. The alkalinity concentration is 6.83 milligrams per liter and the daily load is . At the mouth of Heberly Run, the acidity concentration is 2.80 milligrams per liter and the concentration of alkalinity is 6.93 milligrams per liter. The daily loads for these substances are