Kopi Luwak proteolytic enzymes which break down the beans' proteins, yielding shorter peptides and more free amino acids. The proteins also undergo non-enzymatic Maillard browning reactions in the later roasting process. Moreover, while inside a civet the beans begin to germinate by malting which also lowers their bitterness. Marcone also conducted an analysis on the volatile compounds which are responsible for the coffee's flavour and aroma, showing that there are significant differences from regular coffee. He concluded that: According to Dr. Davila Cortes, the altered protein structure degrades the effectiveness of the coffee as a diuretic. Few objective assessments of taste are
What Hurts the Most hip-swiveling chorus after a flurry of robotic drum beats is certainly a good giggle." Kurt Kirton of About.com named it one of the album's standout tracks. Chuck Taylor of "Billboard" gave "What Hurts the Most" a positive review, writing that "this track is remixed into a frothy uptempo anthem, complete with requisite percussive thump and an exulted vocal (with 12 mixes in all) that leaves the lyric's potential heartbreak in the dust." Taylor ended his review, asking "Wouldn't it be wondrous if American programmers renewed their vow in 2008 to put variety on the airwaves—or do we really need a