Nitrate test to 2.5 micrograms and a concentration of 1 in 25,000 parts. Devarda's alloy (Copper/Aluminium/Zinc) is a reducing agent. When reacted with nitrate in sodium hydroxide solution, ammonia is liberated. The ammonia formed may be detected by its characteristic odor, and by damp red litmus paper's turning blue, signalling that it is an alkali — very few gases other than ammonia evolved from wet chemistry are alkaline. Aluminium is the reducing agent in this reaction that will occur. Diphenylamine may be used as a wet chemical test for the presence of the nitrate ion. In this test, a solution of diphenylamine
Olive oil acidity attacked by flies and parasites), bruised olives, delayed harvesting and storage before processing. The lipolysis reaction is greatly enhanced by the presence of an aqueous phase, so when oil is separated from water during processing, lipolysis slows down and stops. Free acidity is a defect of olive oil that is tasteless and odorless, thus can not be detected by sensory analysis. Since vegetable oils are not aqueous fluids, a pH-meter can not be used for this measure. Various approaches exist that can measure oil acidity with good accuracy. The official technique to measure free acidity in olive oil (as defined