Vapor-compression refrigeration replaced, such as sulfur dioxide. Haloalkanes are also an order(s) of magnitude more expensive than petroleum derived flammable alkanes of similar or better cooling performance. Unfortunately, chlorine- and fluorine-bearing refrigerants reach the upper atmosphere when they escape. In the stratosphere, CFCs break up due to UV radiation, releasing their chlorine free radicals. These chlorine free radicals act as catalysts in the breakdown of ozone through chain reactions. One CFC molecule can cause thousands of ozone molecules to break down. This causes severe damage to the ozone layer that shields the Earth's surface from the Sun's strong UV radiation, and has
Exposing Microorganisms in the Stratosphere stratosphere (31 km above sea level), 99.9% of the entire population was destroyed. According to the researchers, "most terrestrial bacteria would be inactivated within the first [day] on Mars if contaminated spacecraft surfaces receive direct sunlight." Of the 40 million spores exposed to the stratosphere, only 267 spores (or 0.0007%) remained viable. Extrapolating this, no viable spores would remain if flight samples had an additional 150 minutes of Sun exposure in the stratosphere (630 min total time). The survivors showed three single nucleotide (base pair) substitutions compared to unexposed controls kept on the ground. These three coding regions are associated