Francois Fiedler Foundation circle gathered and was joined by a new generation of artists. Fiedler created a unique style in lyrical abstraction. The only European artist, Francois Fiedler was represented in the exhibition "The Persistence of Pollock” celebrating the 100th anniversary of the birth of Jackson Pollock, the titan of Abstract Expressionism. The exhibition was held at the Pollock-Krasner House and Studio, which is the house where Jackson Pollock lived and created the 20th-century abstract expressionism masterpieces, so-called drip painting. “I entered the painting... I felt the dramatic force. The pictorial energy of canvas enchanted me. I felt the full freedom of the
DRIP-seq other methods, such as column-based methods. gDNA is treated with S1 nuclease to remove undesired ssDNA and RNA, followed by ethanol precipitation to remove the S1 nuclease. Then, gDNA is fragmented with restriction endonuclease, yielding double-stranded DNA (dsDNA) fragments of different sizes. Alternatively, gDNA fragments can be generated by sonication. Fragmented gDNA is incubated with the DNA-RNA structure-specific S9.6 mAb. This step is unique for the DRIP-seq protocol, since it entirely relies on the high specificity and affinity of the S9.6 mAb for DNA-RNA hybrids. The antibody will recognize and bind these regions dispersed across the genome and will be