Western painting critic Robert Coates. Abstract expressionism, Action painting, and Color Field painting are synonymous with the New York School. Technically Surrealism was an important predecessor for Abstract expressionism with its emphasis on spontaneous, automatic or subconscious creation. Jackson Pollock's dripping paint onto a canvas laid on the floor is a technique that has its roots in the work of André Masson. Another important ear<nowiki>ly m</nowiki>anifestation of what came to be abstract expressionism is the work of American Northwest artist Mark Tobey, especially his "white writing" canvases, which, though generally not large in scale, anticipate the "all over" look of Pollock's drip
DRIP-seq other methods, such as column-based methods. gDNA is treated with S1 nuclease to remove undesired ssDNA and RNA, followed by ethanol precipitation to remove the S1 nuclease. Then, gDNA is fragmented with restriction endonuclease, yielding double-stranded DNA (dsDNA) fragments of different sizes. Alternatively, gDNA fragments can be generated by sonication. Fragmented gDNA is incubated with the DNA-RNA structure-specific S9.6 mAb. This step is unique for the DRIP-seq protocol, since it entirely relies on the high specificity and affinity of the S9.6 mAb for DNA-RNA hybrids. The antibody will recognize and bind these regions dispersed across the genome and will be