Jackson Pollock Jackson Pollock Paul Jackson Pollock (; January 28, 1912 – August 11, 1956) was an American painter and a major figure in the abstract expressionist movement. He was well known for his unique style of drip painting. During his lifetime, Pollock enjoyed considerable fame and notoriety; he was a major artist of his generation. Regarded as reclusive, he had a volatile personality, and struggled with alcoholism for most of his life. In 1945, he married the artist Lee Krasner, who became an important influence on his career and on his legacy. Pollock died at the age of 44 in an
DRIP-seq other methods, such as column-based methods. gDNA is treated with S1 nuclease to remove undesired ssDNA and RNA, followed by ethanol precipitation to remove the S1 nuclease. Then, gDNA is fragmented with restriction endonuclease, yielding double-stranded DNA (dsDNA) fragments of different sizes. Alternatively, gDNA fragments can be generated by sonication. Fragmented gDNA is incubated with the DNA-RNA structure-specific S9.6 mAb. This step is unique for the DRIP-seq protocol, since it entirely relies on the high specificity and affinity of the S9.6 mAb for DNA-RNA hybrids. The antibody will recognize and bind these regions dispersed across the genome and will be