Aquae Calidae, Bulgaria well as from Kabyle, Mesambria (now Nessebar), Odessos, Histria, Tomis, Byzantium, Abdera, Maroneia and the islands of the Aegean, the oldest from Apollonia Pontica from the 5th century BC. The first baths at the Sanctuary of the Three Nymphs originated when the Roman Empire under Lucullus conquered the region, during the Third Mithridatic War in 72 BC. Under Trajan (98-117) nearby Anchialos became the administrative centre of the region and the baths were expanded and extended with two more swimming pools. During this time the Via Pontica was built connecting Constantinople via Deultum, Anchialos, Aquae Calidae with Dorostorum and Sexaginta
Aphanizomenon flos-aquae (dietary supplement) from the air, and water to synthesize proteins, carbohydrates, and lipids. AFA is also able to directly use molecular nitrogen from the air to produce proteins and other nitrogen-containing biomolecules. This ability is widespread in prokaryotes, but unknown in eukaryotes. "Aphanizomenon flos-aquae" as a species has both toxic and non-toxic forms. While benefits have been indicated, toxicity has been shown in some strains of the species "Aphanizomenon flos-aquae", with cylindrospermopsin and saxitoxins present, and microcystins found contaminating AFA dietary supplements. The World Health Organization's "Guidelines For Drinking Water Quality" note Anatoxin-a, saxitoxins, and cylindrospermopsins are present in the Aphanizomenon genus.