Harold Ramis Dan Aykroyd on the screenplay for "Ghostbusters", which became one of the biggest comedy hits of all time, in which he also starred as Dr. Egon Spengler, a role he reprised for the 1989 sequel, "Ghostbusters II" (which he also co-wrote with Aykroyd). His later film "Groundhog Day" has been called his "masterpiece". His films have been noted for attacking "the smugness of institutional life ... with an impish good [will] that is unmistakably American". They are also noted for "Ramis's signature tongue-in-cheek pep talks". Sloppiness and improv were also important aspects of his work. Ramis frequently depicted the qualities
Pictet–Spengler reaction imine generated from the condensation of the aldehyde and amine. This helps to explain the need for an acidic medium in some substrates, as the imine may not be electrophilic enough for ring closure to occur but the Iminium ion is capable of undergoing the reaction. The Pictet-Spengler reaction is widespread in both human industry and biosynthesis. It has remained an important reaction in the fields of alkaloid and pharmaceutical synthesis since its inception, where it has been employed in the development of many beta-carbolines. The enzyme strictosidine synthase is an example of nature utilizing the Pictet-Spengler reaction, which generates