Egon Spengler the city, after the digging of a new subway tunnel resulted in the release of an ancient ghost, Spengler was forced to recruit his only four current students to act as the new Ghostbusters. Although willing to do his share of the legwork, Spengler overestimates his abilities and his aging becomes apparent when he is no longer able to work at the same level as in his younger days, generally working at the firehouse doing research while the team handle the actual 'Ghostbusting', though when the situation calls for it he will help. Melnitz is still carrying a torch for
Pictet–Spengler reaction imine generated from the condensation of the aldehyde and amine. This helps to explain the need for an acidic medium in some substrates, as the imine may not be electrophilic enough for ring closure to occur but the Iminium ion is capable of undergoing the reaction. The Pictet-Spengler reaction is widespread in both human industry and biosynthesis. It has remained an important reaction in the fields of alkaloid and pharmaceutical synthesis since its inception, where it has been employed in the development of many beta-carbolines. The enzyme strictosidine synthase is an example of nature utilizing the Pictet-Spengler reaction, which generates