Self-contained breathing apparatus pressure changes with ambient temperature. As temperature decreases, the pressure inside the cylinder decreases. The relationship between the temperature and the pressure of a gas is defined by the formula "PV" = "nRT". (See Universal gas constant.) What is particularly important to understand from the formula is that the temperature is in kelvins, not degrees Fahrenheit. Consider the freezing point of water at 32 °F (0 °C, 273.15 K) and compare it to 96 °F (35.6 °C or 308.71 K; normal human body temperature is 37 °C). While 96 is arithmetically three times 32, the difference in temperature from a
Temperature-sensitive mutant Temperature-sensitive mutant Temperature sensitive mutants are variants of genes that allow normal function of the organism at low temperatures, but altered function at higher temperatures. Most temperature sensitive mutations affect proteins, and cause loss of protein function at the non-permissive temperature. The permissive temperature is one at which the protein typically can fold properly, or remain properly folded. At higher temperatures, the protein is unstable and ceases to function properly. These mutations are usually recessive in diploid organisms. The permissive temperature is the temperature at which a temperature sensitive mutant gene product takes on a normal, functional phenotype. When a