HEK 293 cells cell, all of which are abundant in kidneys. However, the original adenovirus transformation was inefficient, suggesting that the cell that finally produced the HEK 293 line may have been unusual in some fashion. Graham and coworkers provided evidence that HEK 293 cells and other human cell lines generated by adenovirus transformation of human embryonic kidney cells have many properties of immature neurons, suggesting that the adenovirus preferentially transformed a neuronal lineage cell in the original kidney culture. A comprehensive study of the genomes and transcriptomes of HEK 293 and five derivative cell lines compared the HEK 293 transcriptome with that
How Many More Years Midnight", described by Phillips as "the most different record I ever heard", was probably recorded at a different session. Phillips had not yet set up Sun Records and regularly leased his recordings to the Chess label in Chicago. The record was issued as Chess 1479 on 15 September 1951, with "Moanin' at Midnight" as the A-side and "How Many More Years" as the B-side. "Moanin' at Midnight" entered the R&B chart on 10 November 1951, and was followed four weeks later by "How Many More Years", which became the more popular side and rose to no.4 on the chart. The