Sonic the Hedgehog platforming of the main levels. The 16-bit "Sonic the Hedgehog " consisted of a giant rotating maze, which many considered a major technical achievement. The most common special stages, however, were segments with the character running through a long tunnel to collect certain items. 3D "collect item" levels, as in "Sonic 3" and "Sonic & Knuckles", used the same perspective but had Sonic collecting all the blue-colored orbs on the surface of a giant sphere. Finally, "Sonic Advance 2" employed a 3D ring-collecting stage, and "Sonic Chaos" ("Sonic and Tails" in Japan) used a variety of gimmicks for its levels.
Sonic hedgehog are thought to be successively patterned throughout the neural tube by the SHH concentration gradient. Mutual inhibition between pairs of transcription factors expressed in neighboring domains contributes to the development of sharp boundaries, however, in some cases, inhibitory relationship has been found even between pairs of transcription factors from more distant domains. Particularly, NKX2-2 expressed in the V3 domain is reported to inhibit IRX3 expressed in V2 and more dorsal domains, although V3 and V2 are separated by a further domain termed MN. Sonic hedgehog (SHH) is a signaling molecule that is encoded by the same gene sonic hedgehog. SHH