Barbie: Life in the Dreamhouse of the show's gags rely on their doll-like nature. The series centers on the life of Barbie, her friends, siblings, her boyfriend, Ken and a number of pets. The series is stylized as a mock reality show featuring confessionals of the characters in between scenes. The show heavily relies on slapstick humor, and makes a lot of satirical and self-parody references to the Barbie doll line. Barbie: Dreamhouse Party is a party video game published by Little Orbit and developed by Torus Games based on the show. Barbie: Life in the Dreamhouse Barbie: Life in the Dreamhouse is an online
S-tag S-tag S-tag is the name of an oligopeptide derived from pancreatic ribonuclease A (RNase A). If RNase A is digested with subtilisin, a single peptide bond is cleaved, but the resulting two products remain weakly bound to each other and the protein, called ribonuclease S, remains active although each of the two products alone shows no enzymatic activity. The N-terminus of the original RNase A, also called S-peptide, consists of 20 amino acid residues, of which only the first 15 are required for ribonuclease activity. This 15 amino acids long peptide is called S15 or S-tag. The amino acid sequence