HEK 293 cells cell, all of which are abundant in kidneys. However, the original adenovirus transformation was inefficient, suggesting that the cell that finally produced the HEK 293 line may have been unusual in some fashion. Graham and coworkers provided evidence that HEK 293 cells and other human cell lines generated by adenovirus transformation of human embryonic kidney cells have many properties of immature neurons, suggesting that the adenovirus preferentially transformed a neuronal lineage cell in the original kidney culture. A comprehensive study of the genomes and transcriptomes of HEK 293 and five derivative cell lines compared the HEK 293 transcriptome with that
A Million Ways to Die in the West and dangerous it must have been to live in the Wild West." MacFarlane, a lifelong fan of westerns, began researching the topic, using Jeff Guinn's nonfiction novel, "The Last Gunfight: The Real Story of the Shootout at the O.K. Corral — And How It Changed the American West" as an "invaluable resource," and basis for many of the ways of dying in the film. Various aspects of the film were inspired by real westerns. The decision to make Albert a sheep herder was inspired by "Montana" (1950) and his average, non-confrontational demeanor by "" (1957). Other westerns that inspired MacFarlane