HEK 293 cells HEK 293 cells Human embryonic kidney 293 cells, also often referred to as HEK 293, HEK-293, 293 cells, or less precisely as HEK cells, are a specific cell line originally derived from human embryonic kidney cells grown in tissue culture. HEK 293 cells have been widely used in cell biology research for many years, because of their reliable growth and propensity for transfection. They are also used by the biotechnology industry to produce therapeutic proteins and viruses for gene therapy. HEK 293 cells were generated in 1973 by transfection of cultures of normal human embryonic kidney cells with sheared adenovirus
A Million Ways to Die in the West and dangerous it must have been to live in the Wild West." MacFarlane, a lifelong fan of westerns, began researching the topic, using Jeff Guinn's nonfiction novel, "The Last Gunfight: The Real Story of the Shootout at the O.K. Corral — And How It Changed the American West" as an "invaluable resource," and basis for many of the ways of dying in the film. Various aspects of the film were inspired by real westerns. The decision to make Albert a sheep herder was inspired by "Montana" (1950) and his average, non-confrontational demeanor by "" (1957). Other westerns that inspired MacFarlane