Fractionation of carbon isotopes in oxygenic photosynthesis product 3-phosphoglycerate is depleted in C due to the kinetic isotope effect of the above reaction. The overall C fractionation for C3 photosynthesis ranges between -20 to -37‰. The wide range of variation in delta values expressed in C3 plants is modulated by the stomatal conductance, or the rate of CO entering, or water vapor exiting, the small pores in the epidermis of a leaf. The δC of C3 plants depends on the relationship between stomatal conductance and photosynthetic rate, which is a good proxy of water use efficiency in the leaf. C3 plants with high water-use efficiency tend to
Photosynthesis cytosol they turn back into CO very slowly without the help of carbonic anhydrase. This causes the HCO ions to accumulate within the cell from where they diffuse into the carboxysomes. Pyrenoids in algae and hornworts also act to concentrate around rubisco. The overall process of photosynthesis takes place in four stages: Plants usually convert light into chemical energy with a photosynthetic efficiency of 3–6%. Absorbed light that is unconverted is dissipated primarily as heat, with a small fraction (1–2%) re-emitted as chlorophyll fluorescence at longer (redder) wavelengths. This fact allows measurement of the light reaction of photosynthesis by using