Fractionation of carbon isotopes in oxygenic photosynthesis be less fractionated in C (ie, δC is relatively less negative) compared to C3 plants with low water-use efficiency. C4 plants have developed the C4 carbon fixation pathway to conserve water loss, thus are more prevalent in hot, sunny, and dry climates. These plants differ from C3 plants because the Calvin Cycle in C4 plants produces a four-carbon molecule, malate (rather than 3-phosphoglycerate), after fixation of CO. Malate, the four-carbon compound is the namesake of "C4" photosynthesis. This pathway allows C4 photosynthesis to efficiently shuttle CO to the RuBisCO enzyme and maintain high concentrations of CO within bundle sheath cells.
Photosynthesis the net photosynthetic rates ranged from 10 to 13 μmol CO·m·s, with the conclusion that all terrestrial plants having the same photosynthetic capacities that were light saturated at less than 50% of sunlight. Later in 1958-1963 at Cornell University, field grown maize was reported to have much greater leaf photosynthetic rates of 40 μmol CO·m·s and was not saturated at near full sunlight. This higher rate in maize was almost double those observed in other species such as wheat and soybean, indicating that large differences in photosynthesis exist among higher plants. At the University of Arizona, detailed gas exchange research