DNA RNA strands are created using DNA strands as a template in a process called transcription. Under the genetic code, these RNA strands specify the sequence of amino acids within proteins in a process called translation. Within eukaryotic cells, DNA is organized into long structures called chromosomes. Before typical cell division, these chromosomes are duplicated in the process of DNA replication, providing a complete set of chromosomes for each daughter cell. Eukaryotic organisms (animals, plants, fungi and protists) store most of their DNA inside the cell nucleus and some in organelles, such as mitochondria or chloroplasts. In contrast, prokaryotes (bacteria and
DNA polymerase epsilon RPA. PCNA and RFC function as anchor and direct DNA polymerase epsilon onto the DNA template. DNA polymerase epsilon DNA polymerase epsilon is a member of the DNA polymerase family of enzymes found in eukaryotes. It is composed of the following four subunits: POLE (central catalytic unit), POLE2 (subunit 2), POLE3 (subunit 3), and POLE4 (subunit 4). Recent evidence suggests that it plays a major role in leading strand DNA synthesis and nucleotide and base excision repair. Research had conducted to study nucleotide excision repair DNA synthesis by DNA polymerase epsilon in the presence of PCNA (proliferating cell nuclear antigen),