Biomolecular structure chemical probing, hydroxyl radical probing, nucleotide analog interference mapping (NAIM), and in-line probing. Protein and nucleic acid structures can be determined using either nuclear magnetic resonance spectroscopy (NMR) or X-ray crystallography or single-particle cryo electron microscopy (cryoEM). The first published reports for DNA (by Rosalind Franklin and Raymond Gosling in 1953) of A-DNA X-ray diffraction patterns—and also B-DNA—used analyses based on Patterson function transforms that provided only a limited amount of structural information for oriented fibers of DNA isolated from calf thymus. An alternate analysis was then proposed by Wilkins et al. in 1953 for B-DNA X-ray diffraction and scattering
Non-helical models of DNA structure image with Raymond Gosling, Maurice Wilkins, Alexander Stokes, and Herbert Wilson, as well as base-pairing chemical and biochemical information by Erwin Chargaff. Wilkins and colleagues also reported on X-ray patterns of the state of "in vivo" B-DNA in partially oriented salmon sperm heads. Crick, Wilkins, and Watson each received one-third of the 1962 Nobel Prize in Physiology or Medicine for their contributions to this discovery. Franklin, whose breakthrough X-ray diffraction data was used to formulate the DNA structure, died in 1958, and thus was ineligible to be nominated for a Nobel Prize. In 1963, John Cairns published autoradiographs of the