Fractionation of carbon isotopes in oxygenic photosynthesis the bundle sheath cells. Different forms of CCM in phytoplankton include the active uptake of bicarbonate and CO through the cell membrane, the active transport of inorganic carbon from the cellular membrane to the chloroplasts, and active, unidirectional conversion of CO to bicarbonate. The parameters affecting C fractionation in phytoplankton contribute to "δ"C values between -18 to -25‰. Fractionation of carbon isotopes in oxygenic photosynthesis Photosynthesis converts carbon dioxide to carbohydrates via several metabolic pathways that provide energy to an organism and preferentially react with certain stable isotopes of carbon. The selective enrichment of one stable isotope over another creates
Fractionation of carbon isotopes in oxygenic photosynthesis However, growth rate alone does not account for observed fractionation. The flux of CO into and out of a cell is roughly proportional to the cell surface area, and the cell carbon biomass varies as a function of cell volume. Phytoplankton geometry that maximizes surface area to volume should have larger isotopic fractionation from photosynthesis. The biochemical characteristics of phytoplankton are similar to C3 plants, whereas the gas exchange characteristics more closely resemble the C4 strategy. More specifically, phytoplankton improve the efficiency of their primary carbon-fixing enzyme, RuBisCO, with carbon concentrating mechanisms (CCM), just as C4 plants accumulate CO in