Maurice Wilkins by James Watson. During the second phase, 1951–52, Wilkins produced clear "B form" "X" shaped images from squid sperm, images he sent to James Watson and Francis Crick, causing Watson to write "Wilkins... has obtained extremely excellent X-ray diffraction photographs" [of DNA]. In 1953 Wilkins' colleague Rosalind Franklin instructed Raymond Gosling to hand over to Wilkins a high quality image of "B" form DNA ("Photo 51"), which she had made in 1952 but had “put it aside” as she was leaving King's College London. Wilkins showed it to Watson. This image, along with the knowledge that Linus Pauling had proposed
DNA supercoil and once again it is (in part at least) relieved by superhelicity, but this time in the opposite direction ("i.e.," left-handed or "positive"). Each left-handed tertiary supertwist removes a single, now "undesirable" right-handed Watson-Crick secondary twist. The titration ends at pH 13, where Form IV appears. DNA supercoil DNA supercoiling refers to the over- or under-winding of a DNA strand, and is an expression of the strain on that strand. Supercoiling is important in a number of biological processes, such as compacting DNA, and by regulating access to the genetic code, DNA supercoiling strongly affects DNA metabolism and possibly gene