Vascular tissue is not surprising that their form should be similar to pipes. The individual cells of phloem are connected end-to-end, just as the sections of a pipe might be. As the plant grows, new vascular tissue differentiates in the growing tips of the plant. The new tissue is aligned with existing vascular tissue, maintaining its connection throughout the plant. The vascular tissue in plants is arranged in long, discrete strands called vascular bundles. These bundles include both xylem and phloem, as well as supporting and protective cells. In stems and roots, the xylem typically lies closer to the interior of the
Plant tissue culture concept of totipotentency of plant cells. However this is not true for all cells or for all plants. In many species explants of various organs vary in their rates of growth and regeneration, while some do not grow at all. The choice of explant material also determines if the plantlets developed via tissue culture are haploid or diploid. Also the risk of microbial contamination is increased with inappropriate explants. The first method involving the meristems and induction of multiple shoots is the preferred method for the micropropagation industry since the risks of somaclonal variation (genetic variation induced in tissue culture)