Photosynthesis algae, photosynthesis takes place in organelles called chloroplasts. A typical plant cell contains about 10 to 100 chloroplasts. The chloroplast is enclosed by a membrane. This membrane is composed of a phospholipid inner membrane, a phospholipid outer membrane, and an intermembrane space. Enclosed by the membrane is an aqueous fluid called the stroma. Embedded within the stroma are stacks of thylakoids (grana), which are the site of photosynthesis. The thylakoids appear as flattened disks. The thylakoid itself is enclosed by the thylakoid membrane, and within the enclosed volume is a lumen or thylakoid space. Embedded in the thylakoid membrane are
Fractionation of carbon isotopes in oxygenic photosynthesis be less fractionated in C (ie, δC is relatively less negative) compared to C3 plants with low water-use efficiency. C4 plants have developed the C4 carbon fixation pathway to conserve water loss, thus are more prevalent in hot, sunny, and dry climates. These plants differ from C3 plants because the Calvin Cycle in C4 plants produces a four-carbon molecule, malate (rather than 3-phosphoglycerate), after fixation of CO. Malate, the four-carbon compound is the namesake of "C4" photosynthesis. This pathway allows C4 photosynthesis to efficiently shuttle CO to the RuBisCO enzyme and maintain high concentrations of CO within bundle sheath cells.