Cell cycle Cell cycle The cell cycle, or cell-division cycle, is the series of events that take place in a cell leading to its division and duplication of its DNA (DNA replication) to produce two daughter cells. In bacteria, which lack a cell nucleus, the cell cycle is divided into the B, C, and D periods. The B period extends from the end of cell division to the beginning of DNA replication. DNA replication occurs during the C period. The D period refers to the stage between the end of DNA replication and the splitting of the bacterial cell into two daughter
Single-cell DNA template strand sequencing cellular processes. Briefly, pulse-chase assays allow researchers to track radioactively labelled molecules in the cell. In experiments used to study non-random chromosome assortment, stem cells are labeled or "pulsed" with a nucleotide analog that is incorporated in the replicated DNA strands. This allows the nascent stands to be tracked through many rounds of replication. Unfortunately, this method is found to have poor resolution as it can only be observed at the chromatid level. CO-FISH, or strand-specific fluorescence "in situ" hybridization, facilitates strand-specific targeting of DNA with fluorescently-tagged probes. It exploits the uniform orientation of major satellites relative to the direction