Cultured pearl is calcium carbonate and a fibrous protein called conchiolin. As the nacre builds up in layers of minute aragonite tablets, it fills the growing pearl sac and eventually forms a pearl. Natural pearls are formed by nature, more or less by chance. On the other hand, cultured pearls are human creations formed by inserting a tissue graft from a donor oyster, upon which a pearl sac forms, and the inner side precipitates calcium carbonate, in the form of nacre or "mother-of-pearl". The most popular and effective method for creating cultured pearls are made from the shells of freshwater river mussels
Pearl are also commonly seen in necklaces. Circled pearls are characterized by concentric ridges, or rings, around the body of the pearl. In general, cultured pearls are less valuable than natural pearls, whereas imitation pearls have almost no value. One way that jewelers can determine whether a pearl is cultured or natural is to have a gemlab perform an X-ray examination of the pearl. If X-rays reveals a nucleus, the pearl is likely a bead-nucleated saltwater pearl. If no nucleus is present, but irregular and small dark inner spots indicating a cavity are visible, combined with concentric rings of organic substance,