Versican other inflammatory cells) can migrate more easily through extracellular matrix that has a higher versican content. Deposits of versican are not present in normal skin but are found in the reticular dermis during keloid scarring, a condition where scar formation becomes uncontrolled and overgrowth of skin tissue occurs at the site of the wound. Versican has been shown to interact with hyaluronan and a link protein (hyaluronan and proteoglycan link protein 1; HAPLN1). Versican Versican is a large extracellular matrix proteoglycan that is present in a variety of human tissues. It is encoded by the "VCAN" gene. Versican is a
Tissue culture the tissue in a well defined environment which can be easily manipulated and analysed. In animal tissue culture, cells may be grown as two-dimensional monolayers (conventional culture) or within fibrous scaffolds or gels to attain more naturalistic three-dimensional tissue-like structures (3D culture). Eric Simon, in a 1988 NIH SBIR grant report, showed that electrospinning could be used to produced nano- and submicron-scale polymeric fibrous scaffolds specifically intended for use as "in vitro" cell and tissue substrates. This early use of electrospun fibrous lattices for cell culture and tissue engineering showed that various cell types would adhere to and proliferate upon