Urtica dioica traces of zeaxanthin or between 20 – 60 mg / kg of dry matter. Nettle contains ascorbic acid (vitamin C), riboflavin (vitamin B), pantothenic acid, vitamin K and tocopherols (vitamin E). The highest vitamin contents can be found in the leaves. In laying hens, nettle can be used as an egg yolk colorant instead of artificial pigments or other natural pigments (derived from marigold for yellow). Nettle has high carotenoid contents, especially lutein, β-carotene and zeaxanthin, of which lutein and zeaxanthin act as yellow pigments. Feeding as little as 6.25 g dry nettle per kg feed is as effective as
Chemistry of ascorbic acid acid solution. Electrolyzing the solution of potassium iodide produces iodine, which reacts with ascorbic acid. The end of process is determined by potentiometric titration in a manner similar to Karl Fischer titration. The amount of ascorbic acid can be calculated by Faraday's law. Another alternative uses "N"-bromosuccinimide (NBS) as the oxidizing agent, in the presence of potassium iodide and starch. The NBS first oxidizes the ascorbic acid; when the latter is exhausted, the NBS liberates the iodine from the potassium iodide, which then forms the blue-black complex with starch. Chemistry of ascorbic acid Ascorbic acid is an organic compound with