Photosynthesis that leads to the ultimate reduction of NADP to NADPH. In addition, this creates a proton gradient (energy gradient) across the chloroplast membrane, which is used by ATP synthase in the synthesis of ATP. The chlorophyll molecule ultimately regains the electron it lost when a water molecule is split in a process called photolysis, which releases a dioxygen (O) molecule as a waste product. The overall equation for the light-dependent reactions under the conditions of non-cyclic electron flow in green plants is: Not all wavelengths of light can support photosynthesis. The photosynthetic action spectrum depends on the type of accessory
Fractionation of carbon isotopes in oxygenic photosynthesis product 3-phosphoglycerate is depleted in C due to the kinetic isotope effect of the above reaction. The overall C fractionation for C3 photosynthesis ranges between -20 to -37‰. The wide range of variation in delta values expressed in C3 plants is modulated by the stomatal conductance, or the rate of CO entering, or water vapor exiting, the small pores in the epidermis of a leaf. The δC of C3 plants depends on the relationship between stomatal conductance and photosynthetic rate, which is a good proxy of water use efficiency in the leaf. C3 plants with high water-use efficiency tend to