Proliferating cell nuclear antigen originally identified as an antigen that is expressed in the nuclei of cells during the DNA synthesis phase of the cell cycle. Part of the protein was sequenced and that sequence was used to allow isolation of a cDNA clone. PCNA helps hold DNA polymerase epsilon (Pol ε) to DNA. PCNA is clamped to DNA through the action of replication factor C (RFC), which is a heteropentameric member of the AAA+ class of ATPases. Expression of PCNA is under the control of E2F transcription factor-containing complexes. Since DNA polymerase epsilon is involved in resynthesis of excised damaged DNA strands during
Single-cell DNA template strand sequencing Single-cell DNA template strand sequencing Single-cell DNA template strand sequencing, or strand-seq, is a technique for the selective sequencing of a daughter cell’s parental template strands. This technique has many applications, including the identification of sister chromatid exchanges in the parental cell prior to segregation, the identification of misoriented contigs during alignment of reads to the reference genome, and the assessment of non-random segregation of sister chromatids. Strand-seq (single strand sequencing) was first described in 2012 as a technique to isolate sequenced reads from parental template strands in single-cell DNA libraries. As a proof of concept study, the authors demonstrated