DNA mediate the function of the genome. Genomic DNA is tightly and orderly packed in the process called DNA condensation, to fit the small available volumes of the cell. In eukaryotes, DNA is located in the cell nucleus, with small amounts in mitochondria and chloroplasts. In prokaryotes, the DNA is held within an irregularly shaped body in the cytoplasm called the nucleoid. The genetic information in a genome is held within genes, and the complete set of this information in an organism is called its genotype. A gene is a unit of heredity and is a region of DNA that influences
Pfu DNA polymerase and (1991)Patent 5,545,552 over "Pfu" itself was granted in August 1996. Pfu DNA polymerase "Pfu" DNA polymerase is an enzyme found in the hyperthermophilic archaeon "Pyrococcus furiosus", where it functions to copy the organism's DNA during cell division. In the laboratory setting, "Pfu" is used to amplify DNA in the polymerase chain reaction (PCR), where the enzyme serves the central function of copying a new strand of DNA during each extension step. "Pfu" DNA polymerase has superior thermostability and proofreading properties compared to "Taq" DNA polymerase. Unlike "Taq" DNA polymerase, "Pfu" DNA polymerase possesses 3' to 5' exonuclease proofreading activity,