Chemistry of ascorbic acid The main use of -ascorbic acid and its salts is as food additives, mostly to combat oxidation. It is approved for this purpose in the EU with E number E300, USA, Australia, and New Zealand) Another major use of -ascorbic acid is as dietary supplement. Natural biosynthesis of vitamin C occurs in many plants, and animals, by a variety of processes. Eighty percent of the world's supply of ascorbic acid is produced in China. Ascorbic acid is prepared in industry from glucose in a method based on the historical Reichstein process. In the first of a five-step process, glucose is
Folinic acid to be metabolized in the liver before it could support growth of "L. citrovorum." The synthesis of citrovorum factor by liver cells in culture was eventually accomplished from pteroylglutamic acid in the presence of suitable concentrations of ascorbic acid. The simultaneous addition of sodium formate to such systems resulted in increased citrovorum factor activity in the cell-free supernatants (producing, as now known, the 5-formyl derivative), and from this method of preparation of large amounts of the factor, its structure as levo-folinic acid (5-formyl tetrahydrofolic acid) was eventually deduced. Folinic acid should be distinguished from folic acid (vitamin B). However, folinic