Proliferating cell nuclear antigen originally identified as an antigen that is expressed in the nuclei of cells during the DNA synthesis phase of the cell cycle. Part of the protein was sequenced and that sequence was used to allow isolation of a cDNA clone. PCNA helps hold DNA polymerase epsilon (Pol ε) to DNA. PCNA is clamped to DNA through the action of replication factor C (RFC), which is a heteropentameric member of the AAA+ class of ATPases. Expression of PCNA is under the control of E2F transcription factor-containing complexes. Since DNA polymerase epsilon is involved in resynthesis of excised damaged DNA strands during
Transfer DNA Transfer DNA The transfer DNA (abbreviated T-DNA) is the transferred DNA of the tumor-inducing (Ti) plasmid of some species of bacteria such as "Agrobacterium tumefaciens" and "[[Agrobacterium rhizogenes](actually an Ri plasmid)]". The T-DNA is transferred from bacterium into the host plant's [[cell nucleus|nuclear]] DNA [[genome]]. The capability of this specialized tumor-inducing (Ti) plasmid is attributed to two essential regions required for DNA transfer to the host cell. As the T-DNA is bordered by 25-base-pair repeats on each end. Transfer is initiated at the right border and terminated at the left border and requires the "vir" genes of the Ti plasmid.