Onion epidermal cell Onion epidermal cell The epidermal cells of onions provide a protective layer against viruses and fungi that may harm the sensitive tissues. Because of their simple structure and transparency they are often used to introduce students to plant anatomy or to demonstrate plasmolysis. The clear epidermal cells exist in a single layer and do not contain chloroplasts, because the onion fruiting body (bulb) is used for storing energy, not photosynthesis. Each plant cell has a cell wall, cell membrane, cytoplasm, nucleus, and a large vacuole. The nucleus is present at the periphery of the cytoplasm. The vacuole is prominent and
Fractionation of carbon isotopes in oxygenic photosynthesis RuBisCO-dependent cell growth rate, and the cell geometry and surface area. The use of bicarbonate and carbon concentrating mechanisms in phytoplankton distinguishes the isotopic fractionation from plant photosynthetic pathways. The difference between intracellular and extracellular CO concentrations reflects the CO demand of a phytoplankton cell, which is dependent on its growth rate. The ratio of carbon demand to supply governs the diffusion of CO into the cell, and is negatively correlated with the magnitude of the carbon fractionation by phytoplankton. Combined, these relationships allow the fractionation between CO and phytoplankton biomass to be used to estimate the phytoplankton growth rates.