Chemical process of decomposition during early decomposition, while others are degraded during later stages of decomposition. During the early stages of decomposition, soft tissue proteins are broken down. These include proteins that: During later stages of decomposition, more resistant tissue proteins are degraded by the effects of putrefaction. These include: Keratin is a protein which is found in skin, hair, and nails. It is most resistant to the enzymes involved in proteolysis and must be broken down by special keratinolytic microorganisms. This is the reason that hair and nails are commonly found with skeletal remains. In general, proteolysis breaks down proteins into: Continuing proteolysis
Protein A due to the high purity and yield which are achieved in a easily and reliably achieved. This forms the basis for a general antibody purification "platform" which simplifies manufacturing operations and reduces the time and effort required to develop purification processes. A typical mAb purification process is shown at right. Albeit the long history of protein A chromatography for the production of antibodies, the process is still being improved today. Continuous chromatography, more precisely periodic counter-current chromatography, enormously increases the productivity of the purification step. Protein A Protein A is a 42 kDa surface protein originally found in the cell