Antibiotic-Antimycotic Antibiotic-Antimycotic Antibiotic-Antimycotic is a solution that is commonly added to cell culture media to prevent contamination by various bacteria and fungi. It generally contains 10,000 units per milliliter penicillin, 10,000 micrograms per milliliter streptomycin, and 25 micrograms per milliliter amphotericin B. Penicillin is a Beta-lactam antibiotic that is effective in inhibiting Gram-positive bacteria. Streptomycin is an aminoglycoside antibiotic which is effective against most Gram-negative bacteria. Amphotericin B is effective against multi-cellular fungi and yeasts. In human cell culture Antibiotic-Antimycotic solution was used to wash follicular aspirate and granulosa lutein cells to avoid microbial contamination during human oocyte retrieval for assisted
Antibiotic sensitivity growth. Other methods to test antimicrobial susceptibility include the Stokes method, Etest (also based on antibiotic diffusion), Agar and Broth dilution methods for minimum inhibitory concentration (MIC) determination. The results of the test are reported on the antibiogram. Ideal antibiotic therapy is based on determination of the aetiological agent and its relevant antibiotic sensitivity. Empiric treatment is often started before laboratory microbiological reports are available when treatment should not be delayed due to the seriousness of the disease. The effectiveness of individual antibiotics varies with the location of the infection, the ability of the antibiotic to reach the site of