Vitamin C megadosage Vitamin C megadosage Vitamin C megadosage is a term describing the consumption or injection of vitamin C (ascorbic acid) in doses well beyond the current Recommended Dietary Allowance of 90 mg/day, and often well beyond the Tolerable upper intake level of 2,000 mg/day. There is no scientific evidence that megadosage helps to cure or prevent cancer, the common cold, or a variety of other medical conditions. Historic advocates of vitamin C megadosage include Linus Pauling, who won the Nobel Prize in Chemistry in 1954. Pauling argued that, due to a non-functional form of L-gulonolactone oxidase, an enzyme required to make
Nucleic acid structure determination short lived in solution they need to be generated. This can be done using HO, ascorbic acid, and Fe(II)-EDTA complex which is attached to the backbone through EDTA. The Fe(II) along with ascorbic acid generates hydroxyl radicals which then can react with the nucleic acid molecules. Hydroxyl radical probing is often used in conjunction with chemical probing of nucleic acid molecules that are thought to associate with proteins, this is due to the modification done by hydroxyl radicals. Hydroxyl radicals attack the ribose/deoxyribose ring and this results in breaking of the phosphate backbone, which is independent of secondary structure, as