Fractionation of carbon isotopes in oxygenic photosynthesis be less fractionated in C (ie, δC is relatively less negative) compared to C3 plants with low water-use efficiency. C4 plants have developed the C4 carbon fixation pathway to conserve water loss, thus are more prevalent in hot, sunny, and dry climates. These plants differ from C3 plants because the Calvin Cycle in C4 plants produces a four-carbon molecule, malate (rather than 3-phosphoglycerate), after fixation of CO. Malate, the four-carbon compound is the namesake of "C4" photosynthesis. This pathway allows C4 photosynthesis to efficiently shuttle CO to the RuBisCO enzyme and maintain high concentrations of CO within bundle sheath cells.
Ghetto Day/What I Need "What I Need" and "100% Pure Love", the album's first single, "ooze with giddy abandon—not to mention juicy grooves that seep deeper into the brain and body upon repeated spins." "What I Need" was claimed by "Spin" Bernstein to have "xeroxed" Clivillés and Cole's 1991 single "A Deeper Love". However, he predicted that it could have been a potential successor to her previous signature hits, "Gypsy Woman" and "Makin' Happy". "Ghetto Day" and "What I Need" were released as a double A-side single in June 1994. The joint release peaked at 40 on the UK Singles Chart and at No.