DNA RNA strands are created using DNA strands as a template in a process called transcription. Under the genetic code, these RNA strands specify the sequence of amino acids within proteins in a process called translation. Within eukaryotic cells, DNA is organized into long structures called chromosomes. Before typical cell division, these chromosomes are duplicated in the process of DNA replication, providing a complete set of chromosomes for each daughter cell. Eukaryotic organisms (animals, plants, fungi and protists) store most of their DNA inside the cell nucleus and some in organelles, such as mitochondria or chloroplasts. In contrast, prokaryotes (bacteria and
Single-cell DNA template strand sequencing Single-cell DNA template strand sequencing Single-cell DNA template strand sequencing, or strand-seq, is a technique for the selective sequencing of a daughter cell’s parental template strands. This technique has many applications, including the identification of sister chromatid exchanges in the parental cell prior to segregation, the identification of misoriented contigs during alignment of reads to the reference genome, and the assessment of non-random segregation of sister chromatids. Strand-seq (single strand sequencing) was first described in 2012 as a technique to isolate sequenced reads from parental template strands in single-cell DNA libraries. As a proof of concept study, the authors demonstrated