John Jacob Abel to successfully isolate the neutral base of epinephrine by adding ammonium to highly concentrated extract, a method Abel himself tried but failed due to the use of low concentration of extract. After Takamine's success, Abel himself remarked, with great candor, "The efforts of years on my part in this once mysterious field of suprarenal, medullary biochemistry, marred by blunders as they were, eventuated, then, in the isolation of the hormone not in the form of the free base but in that of its monobenzoyl derivative." Together with L.G. Rowntree and B.B. Turner, Abel devised what they called a "vividiffusion" apparatus,
John Jacob, Marquess of Montferrat war expenses, John Jacob rebelled; but, after having besieged in Chivasso, he was forced to cease. His state and his prestige were shattered, and he had again to declare himself vassal of Savoy. John Jacob died at Casale Monferrato in 1445, being succeeded by his son John IV. John Jacob married on April 26, 1411 with Joanna of Savoy (1395–1460), daughter of Amadeus VII, Count of Savoy. They had : John Jacob, Marquess of Montferrat John Jacob Palaeologus (Italian: "Giovanni Giacomo Paleologo") (March 23, 1395 – March 12, 1445) was the Margrave of Montferrat from 1418 to 1445. He was