Fractionation of carbon isotopes in oxygenic photosynthesis Calvin cycle during the day, when stomata are closed to prevent water loss, and the light reactions can drive the necessary ATP and NADPH production. This pathway differs from C4 photosynthesis because CAM plants separate carbon by storing fixed CO in vesicles at night, then transporting it for use during the day. Thus, CAM plants temporally concentrate CO to improve Rubsico efficiency, whereas C4 plants spatially concentrate CO in bundle sheath cells. The distribution of plants which use CAM photosynthesis includes epiphytes (e.g., orchids, bromeliads) and xerophytes (e.g., succulents, cacti). In Crassulacean acid metabolism, isotopic fractionation combines the effects of
Ghetto Day/What I Need "What I Need" and "100% Pure Love", the album's first single, "ooze with giddy abandon—not to mention juicy grooves that seep deeper into the brain and body upon repeated spins." "What I Need" was claimed by "Spin" Bernstein to have "xeroxed" Clivillés and Cole's 1991 single "A Deeper Love". However, he predicted that it could have been a potential successor to her previous signature hits, "Gypsy Woman" and "Makin' Happy". "Ghetto Day" and "What I Need" were released as a double A-side single in June 1994. The joint release peaked at 40 on the UK Singles Chart and at No.