Sap by the fact that phloem sap is physiologically extreme in terms of animal digestion, and it is hypothesized that few animals take direct advantage of this because they lack two adaptations that are necessary to enable direct use by animals. These include the existence of a very high ratio of non-essential/essential amino acids in phloem sap for which these adapted Hemiptera insects contain symbiotic microorganisms which can then provide them with essential amino acids; and also insect "tolerance of the very high sugar content and osmotic pressure of phloem sap is promoted by their possession in the gut of sucrase-transglucosidase
Plant tissue culture important in judging which pieces to culture and which to discard. As shoots emerge from a culture, they may be sliced off and rooted with auxin to produce plantlets which, when mature, can be transferred to potting soil for further growth in the greenhouse as normal plants. The specific differences in the regeneration potential of different organs and explants have various explanations. The significant factors include differences in the stage of the cells in the cell cycle, the availability of or ability to transport endogenous growth regulators, and the metabolic capabilities of the cells. The most commonly used tissue explants