Hemolymph of the animal's body and surrounds all cells. It contains hemocyanin, a copper-based protein that turns blue when oxygenated, instead of the iron-based hemoglobin in red blood cells found in vertebrates, thus giving hemolymph a blue-green color rather than the red color of vertebrate blood. When not oxygenated, hemolymph quickly loses its color and appears grey. The hemolymph of lower arthropods, including most insects, is not used for oxygen transport because these animals respirate through other means, such as tracheas, but it does contain nutrients such as proteins and sugars. Muscular movements by the animal during locomotion can facilitate hemolymph
Packed red blood cells the red blood cells using special equipment, such as the IBM 2991 cell processor in a similar manner to washing RBCs. The processing (often termed "manufacture", since the end result is deemed a biologic biopharmaceutical product) and the storage can occur at a collection center and/or a blood bank. RBCs are mixed with an anticoagulant and storage solution which provides nutrients and aims to preserve viability and functionality of the cells (limiting their so-called "storage lesion"), which are stored at refrigerated temperatures for up to 42 days (in the US), except for the rather unusual long-term storage in which case