Photosynthesis that leads to the ultimate reduction of NADP to NADPH. In addition, this creates a proton gradient (energy gradient) across the chloroplast membrane, which is used by ATP synthase in the synthesis of ATP. The chlorophyll molecule ultimately regains the electron it lost when a water molecule is split in a process called photolysis, which releases a dioxygen (O) molecule as a waste product. The overall equation for the light-dependent reactions under the conditions of non-cyclic electron flow in green plants is: Not all wavelengths of light can support photosynthesis. The photosynthetic action spectrum depends on the type of accessory
Photosynthesis water, and the availability of suitable temperatures for carrying out photosynthesis. The process of photosynthesis provides the main input of free energy into the biosphere, and is one of four main ways in which radiation is important for plant life. The radiation climate within plant communities is extremely variable, with both time and space. In the early 20th century, Frederick Blackman and Gabrielle Matthaei investigated the effects of light intensity (irradiance) and temperature on the rate of carbon assimilation. These two experiments illustrate several important points: First, it is known that, in general, photochemical reactions are not affected by temperature.