Blood gas tension constant 0.0031 represents the amount of oxygen dissolved in plasma. The dissolved-oxygen term is generally small relative to the term for hemoglobin-bound oxygen, but becomes significant at very high PO (as in a hyperbaric chamber) or in severe anemia. This is an estimation and does not account for differences in temperature, pH and concentrations of 2,3 DPG. Blood gas tension Blood gas tension refers to the partial pressure of gases in blood. There are several significant purposes for measuring gas tension; the most common gas tensions measured are oxygen tension (PO), the carbon dioxide tension (PCO) and carbon monoxide tension
Hemoglobin at low partial pressures. Animals other than humans use different molecules to bind to hemoglobin and change its O affinity under unfavorable conditions. Fish use both ATP and GTP. These bind to a phosphate "pocket" on the fish hemoglobin molecule, which stabilizes the tense state and therefore decreases oxygen affinity. GTP reduces hemoglobin oxygen affinity much more than ATP, which is thought to be due to an extra hydrogen bond formed that further stabilizes the tense state. Under hypoxic conditions, the concentration of both ATP and GTP is reduced in fish red blood cells to increase oxygen affinity. A variant