Tooth whitening enamel layer may also be gradually thinned or even perforated by the various forms of tooth wear. Teeth may be darkened by a buildup of surface stains (extrinsic staining), which hides the natural tooth color; or the tooth itself may discolor (intrinsic staining). Extrinsic stains can become internalised through enamel defects or cracks or as a result of dentine becoming exposed but most extrinsic stains appear to be deposited on or in the dental pellicle. Causes of extrinsic staining include: Changes in the thickness of the dental hard tissues would result in intrinsic discolouration. There are a few causal factors
S-layer pores with 2–8 nm in diameter. The terminology “S-layer” was used the first time in 1976. The general use was accepted at the "First International Workshop on Crystalline Bacterial Cell Surface Layers, Vienna (Austria)" in 1984, and in the year 1987 S-layers were defined at the European Molecular Biology Organization Workshop on “Crystalline Bacterial Cell Surface Layers”, Vienna as “Two-dimensional arrays of proteinaceous subunits forming surface layers on prokaryotic cells” (see "Preface", page VI in Sleytr "et al. 1988"). For a brief summary on the history of S-layer research see references For many bacteria, the S-layer represents the outermost interaction