Holoaden Holoaden Holoaden (common name: highland frogs) is a small genus of craugastorid frogs endemic to southeastern Brazil. "Holoaden" is the type genus of subfamily Holoadeninae that was erected in 2008 and placed in family Strabomantidae, erected at the same time. Before that, it was in Brachycephalidae. However, later research has led the whole Strabomantidae, Holoadeninae included, to become included in Craugastoridae. This arrangement is not followed by all, and the AmphibiaWeb still keeps Holoadeninae, and consequently "Holoaden", in Strabomantidae. "Holoaden" grow to snout–vent length at most. Head is narrower than the body. Differentiated tympanic membrane and tympanic annulus are absent.
Eggshell membrane membrane is derived commercially from the eggshells of industrial egg processors. In the United States, egg-breaking facilities generate more than 24 billion broken eggshells every year. There are various ways in which the membrane of an eggshell is separated from the shell, including chemical, mechanical, steam, and vacuum processes: see Eggshell and protein membrane separation. It may be prepared for use as a dietary supplement. The isolated membrane is partially hydrolyzed and dried to produce a powder, that is distributed as eggshell membrane (EM) for use as a dietary supplement. Eggshell membrane Eggshell membrane or shell membrane is the clear