Sap activity, which transforms excess ingested sugar into long-chain oligosaccharides." A much larger set of animals do however consume phloem sap by proxy, either "through feeding on the honeydew of phloem-feeding hemipterans. Honeydew is physiologically less extreme than phloem sap, with a higher essential:non-essential amino acid ratio and lower osmotic pressure," or by feeding on the biomass of insects that have grown on more direct ingestion of phloem sap. Maple syrup is made from reduced sugar maple xylem sap. The sap often is harvested from the Sugar Maple, "Acer saccharum". In some countries (e.g., Lithuania, Latvia, Estonia, Finland, Belarus, Russia) harvesting
Tissue culture Tissue culture Tissue culture is the growth of tissues or cells separate from the organism. This is typically facilitated via use of a liquid, semi-solid, or solid growth medium, such as broth or agar. Tissue culture commonly refers to the culture of animal cells and tissues, with the more specific term plant tissue culture being used for plants. The term "tissue culture" was coined by American pathologist Montrose Thomas Burrows. In 1885 Wilhelm Roux removed a section of the medullary plate of an embryonic chicken and maintained it in a warm saline solution for several days, establishing the basic principle