Acoustic microscopy acoustic microscope (CSAM), and C-mode scanning acoustic microscope (C-SAM). More recently acoustic microscopes based around picosecond ultrasonics systems have demonstrated acoustic imaging in cells using sub-optical wavelengths working with ultrasonic frequencies into the multi-GHz. Since the vast majority of acoustic microscopes in use today are C-SAM type instruments, this discussion will be limited to these instruments. Ultrasound is broadly defined as any sound having a frequency above 20 kHz, which is approximately the highest frequency that can be detected by the human ear. However, the acoustic microscopes emit ultrasound ranging from 5 MHz to beyond 400 MHz so that micrometre
Sound quality bandwidth B, can be perfectly described by more than 2B samples per second, allowing perfect reconstruction of the bandwidth-limited analog signal. For example, for human hearing bandwidth between 0 and 20 kHz, audio must be sampled at above 40 kHz. Due to the need for filtering out ultrasonic frequencies resulting from the conversion to an analog signal, in practice slightly higher sample rates are used: 44.1 kHz (CD audio) or 48 kHz (DVD). In PCM, each audio sample describes the sound pressure at an instant in time with a limited precision. The limited accuracy results in quantization error, a form