Fractionation of carbon isotopes in oxygenic photosynthesis the C3 pathway in the daytime and the C4 pathway in the nighttime. At night, when temperature and water loss are lower, the CO diffuses through the stomata and produce malate via phosphenolpyruvate carboxylase. During the following day, stomata are closed, malate is decarboxylated, and CO is fixed by RuBisCO. This process alone is similar to that of C4 plants and yields characteristic C4 fractionation values of approximately -11‰. However, in the afternoon, CAM plants may open their stomata and perform C3 photosynthesis. In daytime alone, CAM plants have approximately -28‰ fractionation, characteristic of C3 plants. These combined effects provide
Photosynthesis At 1000 ppm CO in measuring air, both the C3 and C4 plants had similar leaf photosynthetic rates around 60 μmol CO·m·s indicating the suppression of photorespiration in C3 plants. There are three main factors affecting photosynthesis and several corollary factors. The three main are: Total photosynthesis is limited by a range of environmental factors. These include the amount of light available, the amount of leaf area a plant has to capture light (shading by other plants is a major limitation of photosynthesis), rate at which carbon dioxide can be supplied to the chloroplasts to support photosynthesis, the availability of