Vitamin C been observed that while serum or blood plasma concentrations follow a circadian rhythm or reflect short-term dietary impact, content within tissues is more stable and can give a better view of the availability of ascorbate within the entire organism. However, very few hospital laboratories are adequately equipped and trained to carry out such detailed analyses. The vast majority of animals and plants are able to synthesize vitamin C, through a sequence of enzyme-driven steps, which convert monosaccharides to vitamin C. Yeasts do not make -ascorbic acid but rather its stereoisomer, erythorbic acid In plants, this is accomplished through the conversion
Chemistry of ascorbic acid acid. American and Chinese researchers are competing to engineer a mutant that can carry out a one-pot fermentation directly from glucose to 2-KGA, bypassing both the need for a second fermentation and the need to reduce glucose to sorbitol. There exists a -ascorbic acid, which does not occur in nature but can be synthesized artificially. To be specific, -ascorbate is known to participate in many specific enzyme reactions that require the correct enantiomer (-ascorbate and not -ascorbate). -Ascorbic acid has a specific rotation of [α] = +23°. The traditional way to analyze the ascorbic acid content is the process of