I-motif DNA I-motif DNA i-motif DNA is a type of deoxyribonucleic acid, discovered in 2018, that is found in the nuclei of human cells. An abbreviation of intercalated motif, the four-strand i-motif is unlike the iconic double helix DNA. I-motifs are four-stranded quadruplex structures formed by cytosine-rich DNA, similar to the G-quadruplex structures that guanine-rich DNA forms. C-rich DNA regions are common in gene regulation portions of the genome. Recently, i-motifs were discovered in human cells and were shown to play a role in cell reproduction. I-motifs have potential applications in nanotechnology and nanomedicine due to their unique pH sensitivity and have
Single-cell DNA template strand sequencing the ability to acquire sequence information from the Watson and/or Crick chromosomal strands in an individual DNA library, depending on the mode of chromatid segregation; a typical DNA library will always contain DNA from both strands. The authors were specifically interested in showing the utility of strand-seq in detecting sister chromatid exchanges (SCEs) at high-resolution. They successfully identified eight putative SCEs in the murine (mouse) embryonic stem (meS) cell line with resolution up to 23 bp. This methodology has also been shown to hold great utility in discerning patterns of non-random chromatid segregation, especially in stem cell lineages. Furthermore, SCEs