Nuclear DNA the bases are held together by hydrogen bonds. Nuclear DNA and mitochondrial DNA differ in many ways, starting with location and structure. Nuclear DNA is located within the nucleus of eukaryote cells and usually has two copies per cell while mitochondrial DNA is located in the mitochondria and contains 100-1,000 copies per cell. The structure of nuclear DNA chromosomes is linear with open ends and includes 46 chromosomes containing 3 billion nucleotides. Mitochondrial DNA chromosomes usually have closed, circular structures, and contain for example 16,569 nucleotides in human. Nuclear DNA is diploid, ordinarily inheriting the DNA from two parents, while
Single-cell DNA template strand sequencing Single-cell DNA template strand sequencing Single-cell DNA template strand sequencing, or strand-seq, is a technique for the selective sequencing of a daughter cell’s parental template strands. This technique has many applications, including the identification of sister chromatid exchanges in the parental cell prior to segregation, the identification of misoriented contigs during alignment of reads to the reference genome, and the assessment of non-random segregation of sister chromatids. Strand-seq (single strand sequencing) was first described in 2012 as a technique to isolate sequenced reads from parental template strands in single-cell DNA libraries. As a proof of concept study, the authors demonstrated