Fractionation of carbon isotopes in oxygenic photosynthesis product 3-phosphoglycerate is depleted in C due to the kinetic isotope effect of the above reaction. The overall C fractionation for C3 photosynthesis ranges between -20 to -37‰. The wide range of variation in delta values expressed in C3 plants is modulated by the stomatal conductance, or the rate of CO entering, or water vapor exiting, the small pores in the epidermis of a leaf. The δC of C3 plants depends on the relationship between stomatal conductance and photosynthetic rate, which is a good proxy of water use efficiency in the leaf. C3 plants with high water-use efficiency tend to
Photosynthesis allowing carbon fixation to 3-phosphoglycerate by RuBisCO. Sixteen thousand species of plants use CAM. Cyanobacteria possess carboxysomes, which increase the concentration of around RuBisCO to increase the rate of photosynthesis. An enzyme, carbonic anhydrase, located within the carboxysome releases CO from the dissolved hydrocarbonate ions (HCO). Before the CO diffuses out it is quickly sponged up by RuBisCO, which is concentrated within the carboxysomes. HCO ions are made from CO outside the cell by another carbonic anhydrase and are actively pumped into the cell by a membrane protein. They cannot cross the membrane as they are charged, and within the