Fractionation of carbon isotopes in oxygenic photosynthesis the C3 pathway in the daytime and the C4 pathway in the nighttime. At night, when temperature and water loss are lower, the CO diffuses through the stomata and produce malate via phosphenolpyruvate carboxylase. During the following day, stomata are closed, malate is decarboxylated, and CO is fixed by RuBisCO. This process alone is similar to that of C4 plants and yields characteristic C4 fractionation values of approximately -11‰. However, in the afternoon, CAM plants may open their stomata and perform C3 photosynthesis. In daytime alone, CAM plants have approximately -28‰ fractionation, characteristic of C3 plants. These combined effects provide
Photosynthesis pigments present. For example, in green plants, the action spectrum resembles the absorption spectrum for chlorophylls and carotenoids with absorption peaks in violet-blue and red light. In red algae, the action spectrum is blue-green light, which allows these algae to use the blue end of the spectrum to grow in the deeper waters that filter out the longer wavelengths (red light) used by above ground green plants. The non-absorbed part of the light spectrum is what gives photosynthetic organisms their color (e.g., green plants, red algae, purple bacteria) and is the least effective for photosynthesis in the respective organisms. In