Inert gas asphyxiation lacks oxygen and consists of an asphyxiant gas (one or more of argon, nitrogen or carbon dioxide), causing the animals to lose consciousness. Argon and nitrogen are important components of a gassing process which seem to cause no pain, and for this reason many consider some types of controlled atmosphere killing more humane than other methods of killing. However, "stunning" is often done using carbon dioxide. If carbon dioxide is used, controlled atmosphere killing is not the same as inert gas asphyxia, because carbon dioxide at high concentrations (above 5%) is not biologically inert, but rather is toxic and also
Bottled gas some variation due to intermolecular force and molecule size compared to an ideal gas. Normal high pressure gas cylinders or bottles will hold from 200 to 400 atmosphere (unit)s. The atmosphere units pressure held by the bottle is equivalent to the number of volumes of standard temperature and pressure of the gas held by the bottle for an ideal gas. Because the contents are under high pressure and are sometimes hazardous, there are special safety regulations for handling bottled gases. These include chaining bottles to prevent falling and breaking, proper ventilation to prevent injury or death in case of leaks