Cell growth briefly at M phase when the nucleus and then the cell divide in two. The process of cell division, called cell cycle, has four major parts called phases. The first part, called G phase is marked by synthesis of various enzymes that are required for DNA replication. The second part of the cell cycle is the S phase, where DNA replication produces two identical sets of chromosomes. The third part is the G phase in which a significant protein synthesis occurs, mainly involving the production of microtubules that are required during the process of division, called mitosis. The fourth phase,
Single-cell DNA template strand sequencing Single-cell DNA template strand sequencing Single-cell DNA template strand sequencing, or strand-seq, is a technique for the selective sequencing of a daughter cell’s parental template strands. This technique has many applications, including the identification of sister chromatid exchanges in the parental cell prior to segregation, the identification of misoriented contigs during alignment of reads to the reference genome, and the assessment of non-random segregation of sister chromatids. Strand-seq (single strand sequencing) was first described in 2012 as a technique to isolate sequenced reads from parental template strands in single-cell DNA libraries. As a proof of concept study, the authors demonstrated