Sap transport proteins. Recent evidence indicates that mobile proteins and RNA are part of the plant's long-distance communication signaling system. Evidence also exists for the directed transport and sorting of macromolecules as they pass through plasmodesmata." A large number of insects of the order Hemiptera (the half-wings), feed directly on phloem sap, and make it the primary component of their diet. Phloem sap is "nutrient-rich compared with many other plant products and generally lacking in toxins and feeding deterrents, [yet] it is consumed as the dominant or sole diet by a very restricted range of animals". This apparent paradox is explained
Plant tissue culture concept of totipotentency of plant cells. However this is not true for all cells or for all plants. In many species explants of various organs vary in their rates of growth and regeneration, while some do not grow at all. The choice of explant material also determines if the plantlets developed via tissue culture are haploid or diploid. Also the risk of microbial contamination is increased with inappropriate explants. The first method involving the meristems and induction of multiple shoots is the preferred method for the micropropagation industry since the risks of somaclonal variation (genetic variation induced in tissue culture)