Peripheral blood mononuclear cell Peripheral blood mononuclear cell A peripheral blood mononuclear cell (PBMC) is any peripheral blood cell having a round nucleus. These cells consist of lymphocytes (T cells, B cells, NK cells) and monocytes, whereas erythrocytes and platelets have no nuclei, and granulocytes (neutrophils, basophils, and eosinophils) have multi-lobed nuclei. In humans, lymphocytes make up the majority of the PBMC population, followed by monocytes, and only a small percentage of dendritic cells. These cells can be extracted from whole blood using ficoll, a hydrophilic polysaccharide that separates layers of blood, and gradient centrifugation, which will separate the blood into a top layer
Blood red dark red state, but quickly oxygenates upon exposure to air, turning a brighter shade of red. This happens more quickly with smaller volumes of blood such as a pinprick and less quickly from cuts or punctures that cause greater blood flows such as a puncture in the basilic vein: all blood collected during a phlebotomy procedure is deoxygenated blood, and it does not usually have a chance to become oxygenated upon leaving the body. Arterial blood, which is already oxygenated, is also already a brighter shade of red— this is the blood see from a pulsating neck, arm, or leg