The Great White Hope (film) The Great White Hope (film) The Great White Hope is a 1970 American biographical romantic drama film written and adapted from the Howard Sackler play of the same name. The film was directed by Martin Ritt, starring James Earl Jones, Jane Alexander, Chester Morris, Hal Holbrook, Beah Richards and Moses Gunn. Jones and Alexander, who also appeared in the same roles in the stage versions, both received Best Actor and Actress Academy Award nominations for their performances. The film and play is based on the true story of Jack Johnson and his first wife, Etta Terry Duryea, and the controversy
Expression vector "lac" or "lac"-derived promoters are based on the "lac"UV5 mutant which is insensitive to catabolite repression. This mutant allows for expression of protein under the control of the "lac" promoter when the growth medium contains glucose since glucose would inhibit gene expression if wild-type "lac" promoter is used. Presence of glucose nevertheless may still be used to reduce background expression through residual inhibition in some systems. Examples of "E. coli" expression vectors are the pGEX series of vectors where glutathione S-transferase is used as a fusion partner and gene expression is under the control of the tac promoter, and the