Jack London character is based on Hawaiian leper Kaluaikoolau, who in 1893 revolted and resisted capture from forces of the Provisional Government of Hawaii in the Kalalau Valley. An amateur boxer and avid boxing fan, London reported on the 1910 Johnson–Jeffries fight, in which the black boxer Jack Johnson vanquished Jim Jeffries, known as the "Great White Hope". In 1908, London had reported on an earlier fight of Johnson's, contrasting the black boxer's coolness and intellectual style, with the apelike appearance and fighting style of his Canadian opponent, Tommy Burns, 'what . . . [won] on Saturday was bigness, coolness, quickness, cleverness,
Expression vector "lac" or "lac"-derived promoters are based on the "lac"UV5 mutant which is insensitive to catabolite repression. This mutant allows for expression of protein under the control of the "lac" promoter when the growth medium contains glucose since glucose would inhibit gene expression if wild-type "lac" promoter is used. Presence of glucose nevertheless may still be used to reduce background expression through residual inhibition in some systems. Examples of "E. coli" expression vectors are the pGEX series of vectors where glutathione S-transferase is used as a fusion partner and gene expression is under the control of the tac promoter, and the