The Great White Hope (film) over their marriage and Duryea's death by suicide in 1912. Set between 1910 and 1915, the story follows Jack Jefferson, patterned after real-life boxer, Jack Johnson, going on a hot streak of victories in the boxing ring as he defeats every white boxer around. Soon the press and racists announce the search for a "great white hope", a boxer who will defeat Jefferson for the heavyweight title. Meanwhile, Jefferson prepares for a few more matches, but he lets his guard down by courting the beautiful, and very white, Eleanor Bachman, and when everyone, including Jack's black "wife", discover this, the
Expression vector "lac" or "lac"-derived promoters are based on the "lac"UV5 mutant which is insensitive to catabolite repression. This mutant allows for expression of protein under the control of the "lac" promoter when the growth medium contains glucose since glucose would inhibit gene expression if wild-type "lac" promoter is used. Presence of glucose nevertheless may still be used to reduce background expression through residual inhibition in some systems. Examples of "E. coli" expression vectors are the pGEX series of vectors where glutathione S-transferase is used as a fusion partner and gene expression is under the control of the tac promoter, and the