The Great White Hope (film) here. Jones later contributed commentary to a documentary about Jack Johnson that would sum up this film, saying: "To know the story of Jack Johnson is to know that it is a study in hubris." However, critical opinion of the film has declined in recent years. "The Great White Hope" currently maintains a 43% "Rotten" approval rating on Rotten Tomatoes from 7 reviews, indicating mixed-to-negative reviews. Critic Vincent Canby referred to the film as "One of those liberal, well-meaning, fervently uncontroversial works that pretend to tackle contemporary problems by finding analogies at a safe remove in history" while critic Emanuel
Expression vector "lac" or "lac"-derived promoters are based on the "lac"UV5 mutant which is insensitive to catabolite repression. This mutant allows for expression of protein under the control of the "lac" promoter when the growth medium contains glucose since glucose would inhibit gene expression if wild-type "lac" promoter is used. Presence of glucose nevertheless may still be used to reduce background expression through residual inhibition in some systems. Examples of "E. coli" expression vectors are the pGEX series of vectors where glutathione S-transferase is used as a fusion partner and gene expression is under the control of the tac promoter, and the