Compulsory voting , 22 countries were recorded as having compulsory voting. Of these, only 10 countries (additionally one Swiss canton and one Indian state) enforce it. Of the 30 member states of the Organisation for Economic Co-operation and Development, 10 had forms of compulsory voting. These are the countries and sub-national entities that enforce compulsory voting: Countries that have compulsory voting by law but do not enforce it: Although voting in a country may be compulsory, penalties for failing to vote are not always strictly enforced. In Australia and Brazil, providing a legitimate reason for not voting (such as illness) is accepted.
Catalytic triad activity was strongly reduced, but was able to be restored by directed evolution. Non-catalytic proteins have been used as scaffolds, having catalytic triads inserted into them which were then improved by directed evolution. The Ser-His-Asp triad has been inserted into an antibody, as well as a range of other proteins. Similarly, catalytic triad mimics have been created in small organic molecules like diaryl diselenide, and displayed on larger polymers like Merrifield resins, and self-assembling short peptide nanostructures. The sophistication of the active site network causes residues involved in catalysis (and residues in contact with these) to be highly evolutionarily conserved.