Compulsory voting papers. Compulsory voting is increasingly resented by citizens in some countries such as Brazil, the largest country where compulsory voting is enforced: at the last presidential election in 2014, some 30 million voters, about 21% of registered voters, did not vote, despite the fact that Brazil has some of the most severe penalties enforced against non voters. A study of a Swiss canton where compulsory voting was enforced found that compulsory voting significantly increased electoral support for leftist policy positions in referenda by up to 20 percentage points. Another study found that the effects of universal turnout in the United
Catalytic triad activity was strongly reduced, but was able to be restored by directed evolution. Non-catalytic proteins have been used as scaffolds, having catalytic triads inserted into them which were then improved by directed evolution. The Ser-His-Asp triad has been inserted into an antibody, as well as a range of other proteins. Similarly, catalytic triad mimics have been created in small organic molecules like diaryl diselenide, and displayed on larger polymers like Merrifield resins, and self-assembling short peptide nanostructures. The sophistication of the active site network causes residues involved in catalysis (and residues in contact with these) to be highly evolutionarily conserved.