Polonium subjects were chosen because experimenters wanted persons who had not been exposed to polonium either through work or accident. All subjects had incurable diseases. Excretion of polonium was followed, and an autopsy was conducted at that time on the deceased patient to determine which organs absorbed the polonium. Patients' ages ranged from 'early thirties' to 'early forties'. The experiments were described in Chapter 3 of Biological Studies with Polonium, Radium, and Plutonium, National Nuclear Energy Series, Volume VI-3, McGraw-Hill, New York, 1950. Not specified is the isotope under study, but at the time polonium-210 was the most readily available polonium
Polonium methylated in living things to create organometallic compounds. Studies investigating the metabolism of polonium-210 in rats have shown that only 0.002 to 0.009% of polonium-210 ingested is excreted as volatile polonium-210. The median lethal dose (LD) for acute radiation exposure is about 4.5 Sv. The committed effective dose equivalent Po is 0.51 µSv/Bq if ingested, and 2.5 µSv/Bq if inhaled. So a fatal 4.5 Sv dose can be caused by ingesting , about 50 nanograms (ng), or inhaling , about 10 ng. One gram of Po could thus in theory poison 20 million people of whom 10 million would die.