Frederick Banting in the blood which was then excreted in urine. Attempts to extract insulin from ground-up pancreas cells were unsuccessful, likely because of the destruction of the insulin by the proteolysis enzyme of the pancreas. The challenge was to find a way to extract insulin from the pancreas prior to its being destroyed. Moses Barron published an article in 1920 which described experimental closure of the pancreatic duct by ligature; this further influenced Banting's thinking. The procedure caused deterioration of the cells of the pancreas that secrete trypsin which breaks down insulin, but it left the islets of Langerhans intact. Banting
Highest and best use both "as improved" and "as if vacant". Vacant properties are generally only given the "as vacant" test. The Highest and Best Use as vacant may be the same or different as the Highest and Best use as improved. For improved properties, the first analysis applied is the use of the property based on the assumption that the parcel is vacant. Then, the analysis focuses on the use that should be made of the property as it is currently improved. The extent to which the existing improvements differ from the highest and best use as-if vacant, reveals the extent of any