Frederick Banting realized that this procedure would destroy the trypsin-secreting cells but not the insulin. Once the trypsin-secreting cells had died, insulin could be extracted from the islets of Langerhans. Banting discussed this approach with J. J. R. Macleod, Professor of Physiology at the University of Toronto. Macleod provided experimental facilities and the assistance of one of his students, Charles Best. Banting and Best, with the assistance of biochemist James Collip, began the production of insulin by this means. As the experiments proceeded, the required quantities could no longer be obtained by performing surgery on living dogs. In November 1921, Banting hit
Highest and best use higher values for their non-timber amenities such as for recreation and conservation.” Organizations such as the American Society of Farm Managers and Rural Appraisers (ASFMRA), the American Society of Appraisers (ASA) and the Appraisal Institute have issued guidance to their members stating that the use of non-economic valuation concepts such as the above must not be used in connection with concepts such as Market Value and Highest and Best Use when engaging in typical appraisal assignments. However, proponents of alternate valuation metrics point out that valuing land through a purely economic perspective often fails to capture important benefits to the