Single-stranded oligonucleotides (ssODNs) of up to 200-nt can be ordered from Integrated DNA Technologies and other companies. Do not have the oligonucleotide PAGE-purified, as this can introduce trace chemical contaminants that reduce embryo viability.
Smoking the brisket. Place the brisket fat side up on the top rack, cover with the lid and bring the temperature up to 225Â°F, using the vents to regulate the temperature. Knowing the temp in your grill is crucial, so if your grill doesnât have a temperature gauge, purchase a digital BBQ thermometer. 4. Low and slow.
Start the Traeger grill on Smoke with the lid open for 4-5 minutes to establish the fire. While waiting, season the brisket on all sides with Prime Rib Rub (reserve 1/2 tablespoon of the seasoning for later use). After the brisket has smoked for 2 hours, remove to a large sheet of plastic wrap.