Single-stranded oligonucleotides (ssODNs) of up to 200-nt can be ordered from Integrated DNA Technologies and other companies. Do not have the oligonucleotide PAGE-purified, as this can introduce trace chemical contaminants that reduce embryo viability.
Smoking the brisket. Place the brisket fat side up on the top rack, cover with the lid and bring the temperature up to 225Â°F, using the vents to regulate the temperature. Knowing the temp in your grill is crucial, so if your grill doesnât have a temperature gauge, purchase a digital BBQ thermometer. 4. Low and slow.
I use a crisscrossed layer of aluminum foil to wrap the smoked brisket. Center the flat on the foil and bring the edges up and around the brisket. Before closing the foil, pour in 1 cup of beef broth for additional moisture. Seal the foil tight and place the smoked brisket back on the smoker. At this point time is out the window.