Single-stranded oligonucleotides (ssODNs) of up to 200-nt can be ordered from Integrated DNA Technologies and other companies. Do not have the oligonucleotide PAGE-purified, as this can introduce trace chemical contaminants that reduce embryo viability.
Smoking the brisket. Place the brisket fat side up on the top rack, cover with the lid and bring the temperature up to 225Â°F, using the vents to regulate the temperature. Knowing the temp in your grill is crucial, so if your grill doesnât have a temperature gauge, purchase a digital BBQ thermometer. 4. Low and slow.
Of course, knowing your smoker well is the secret to success. Adjust the smoker to hold a temperature around 225 degrees F (110 degrees C), for...MORE the entire cooking time. Remember that the brisket needs to cook anywhere between 1 1/2 to 1 hour per pound.