Single-stranded oligonucleotides (ssODNs) of up to 200-nt can be ordered from Integrated DNA Technologies and other companies. Do not have the oligonucleotide PAGE-purified, as this can introduce trace chemical contaminants that reduce embryo viability.
Smoking the brisket. Place the brisket fat side up on the top rack, cover with the lid and bring the temperature up to 225Â°F, using the vents to regulate the temperature. Knowing the temp in your grill is crucial, so if your grill doesnât have a temperature gauge, purchase a digital BBQ thermometer. 4. Low and slow.
Before we get into how to smoke a brisket, let's buy one. For tender, juicy, flavorful brisket, choose one that's untrimmed. It'll have a layer of fat on it that may be up to one inch thick. Look for one that has a quarter-inch to half-inch thick layer of fat.If it's thicker, you can trim some off later.efore we get into how to smoke a brisket, let's buy one. For tender, juicy, flavorful brisket, choose one that's untrimmed. It'll have a layer of fat on it that may be up to one inch thick. Look for one that has a quarter-inch to half-inch thick layer of fat. If it's thicker, you can trim some off later.