Single-stranded oligonucleotides (ssODNs) of up to 200-nt can be ordered from Integrated DNA Technologies and other companies. Do not have the oligonucleotide PAGE-purified, as this can introduce trace chemical contaminants that reduce embryo viability.
Smoking the brisket. Place the brisket fat side up on the top rack, cover with the lid and bring the temperature up to 225Â°F, using the vents to regulate the temperature. Knowing the temp in your grill is crucial, so if your grill doesnât have a temperature gauge, purchase a digital BBQ thermometer. 4. Low and slow.
Smoking the Brisket: Preheat Smoker Grill to 170 degrees F. Cooking at a low temperature will make the wood smoke more. Place the brisket (in roasting pan) in the pre-heated smoker and close the smoker lid. Smoke the brisket for 1 hour for every pound of meat or smoke overnight.inish cooking the brisket: Turn up the temperature of the smoker grill to 180 degrees F. Pour 1 1/2 to 2 cups of apple cider or apple juice into the roasting pan until entire bottom of roasting pan is covered with the liquid. Insert the meat thermometer probe into the center of the brisket.