The indirect Coombs' test is done on a sample of the liquid part of the blood. It detects antibodies that are present in the bloodstream and could bind to certain red blood cells and destroy them. A Coombs' test involves taking a sample of blood.
It is placed in the middle of the chest or thoracic cavity (middle mediastinum), posterior to the body of sternum and the second to sixth costal cartilages and anterior to the fifth to the eighth thoracic vertebrae.
Best Answer: the heart is actually located in the center of your chest right behind the breast bone (sternum). The left side of the heart is slightly larger and therefore the heart tilts to the left side. If you look at an exray you can see the outline of the heart as it sits in your chest.