3. Stationary phase. As the bacterial population continues to grow, all the nutrients in the growth medium are used up by the microorganism for their rapid multiplication. This result in the accumulation of waste materials, toxic metabolites and inhibitory compounds such as antibiotics in the medium.he time taken by the bacteria to double in number during a specified time period is known as the generation time. The generation time tends to vary with different organisms. E.coli divides in every 20 minutes, hence its generation time is 20 minutes, and for Staphylococcus aureus it is 30 minutes.
Quick Answer. Refried beans potentially go bad if they are left in the refrigerator for more than four days. The beans retain their best quality for one to two months in the freezer. Continue Reading.
Someone had a saying  If there's a doubt toss it out . Yeah cabbage can spoil and most foods do after three days. The rule on leftovers is three days. If you were to get food poisoning you would know and you wouldn't risk it again!!! I wouldn't eat it if it's over three days old.