Single-stranded oligonucleotides (ssODNs) of up to 200-nt can be ordered from Integrated DNA Technologies and other companies. Do not have the oligonucleotide PAGE-purified, as this can introduce trace chemical contaminants that reduce embryo viability.
Smoking the brisket. Place the brisket fat side up on the top rack, cover with the lid and bring the temperature up to 225Â°F, using the vents to regulate the temperature. Knowing the temp in your grill is crucial, so if your grill doesnât have a temperature gauge, purchase a digital BBQ thermometer. 4. Low and slow.
Directions. Preheat the oven or smoker to 225 degrees F. Trim the fat evenly across the top to 1/4-inch thick. Season the brisket liberally with the seasoning rub. Cook in the oven or smoker until the internal temperature on an instant-read meat thermometer reaches 175 degrees F, about 6 to 8 hours.Remove the meat from the oven or smoker and wrap in a double layer of aluminum foil to seal in the juices.eason the brisket liberally with the seasoning rub. Cook in the oven or smoker until the internal temperature on an instant-read meat thermometer reaches 175 degrees F, about 6 to 8 hours.