The indirect Coombs' test is done on a sample of the liquid part of the blood. It detects antibodies that are present in the bloodstream and could bind to certain red blood cells and destroy them. A Coombs' test involves taking a sample of blood.
It is placed in the middle of the chest or thoracic cavity (middle mediastinum), posterior to the body of sternum and the second to sixth costal cartilages and anterior to the fifth to the eighth thoracic vertebrae.
The heart is a muscular organ about the size of a fist, located just behind and slightly left of the breastbone. The heart pumps blood through the network of arteries and veins called the cardiovascular system. The heart has four chambers: 1  The right atrium receives blood from the veins and pumps it to the right ventricle.  The right ventricle receives blood from the right atrium and pumps it to the lungs, where it is loaded with oxygen.