3. Stationary phase. As the bacterial population continues to grow, all the nutrients in the growth medium are used up by the microorganism for their rapid multiplication. This result in the accumulation of waste materials, toxic metabolites and inhibitory compounds such as antibiotics in the medium.he time taken by the bacteria to double in number during a specified time period is known as the generation time. The generation time tends to vary with different organisms. E.coli divides in every 20 minutes, hence its generation time is 20 minutes, and for Staphylococcus aureus it is 30 minutes.
Quick Answer. Refried beans potentially go bad if they are left in the refrigerator for more than four days. The beans retain their best quality for one to two months in the freezer. Continue Reading.
This is because canned green beans have a much longer shelf life than do frozen green beans. Frozen green beans stay good between 12 and 18 months in the freezer. Experts say that low acid canned foods such as green beans are probably OK to eat up to 5 years.