Single-stranded oligonucleotides (ssODNs) of up to 200-nt can be ordered from Integrated DNA Technologies and other companies. Do not have the oligonucleotide PAGE-purified, as this can introduce trace chemical contaminants that reduce embryo viability.
Smoking the brisket. Place the brisket fat side up on the top rack, cover with the lid and bring the temperature up to 225Â°F, using the vents to regulate the temperature. Knowing the temp in your grill is crucial, so if your grill doesnât have a temperature gauge, purchase a digital BBQ thermometer. 4. Low and slow.
1 After you have a good smoke in your smoker add the brisket. 2  General rule of thumb is to smoke for about 1.5 hours per pound of brisket. 3  Your time may be a little less or more. Throughout the day, this liquid evaporates to help keep your brisket moist. 2  After you have a good smoke in your smoker add the brisket. 3  General rule of thumb is to smoke for about 1.5 hours per pound of brisket.