Single-stranded oligonucleotides (ssODNs) of up to 200-nt can be ordered from Integrated DNA Technologies and other companies. Do not have the oligonucleotide PAGE-purified, as this can introduce trace chemical contaminants that reduce embryo viability.
Smoking the brisket. Place the brisket fat side up on the top rack, cover with the lid and bring the temperature up to 225Â°F, using the vents to regulate the temperature. Knowing the temp in your grill is crucial, so if your grill doesnât have a temperature gauge, purchase a digital BBQ thermometer. 4. Low and slow.
How to grill brisket. 1  Before cooking, trim fat to 1/4-inch thickness. Then marinate the brisket overnight (12 to 24 hours). 2  Sear brisket directly over medium coals or near a hot fire: 20 minutes per side. After searing, allow approximately 1 hour of cooking time per pound. 3  Slow cook at a low temperature of 250  ËF.