Single-stranded oligonucleotides (ssODNs) of up to 200-nt can be ordered from Integrated DNA Technologies and other companies. Do not have the oligonucleotide PAGE-purified, as this can introduce trace chemical contaminants that reduce embryo viability.
Smoking the brisket. Place the brisket fat side up on the top rack, cover with the lid and bring the temperature up to 225Â°F, using the vents to regulate the temperature. Knowing the temp in your grill is crucial, so if your grill doesnât have a temperature gauge, purchase a digital BBQ thermometer. 4. Low and slow.
When the brisket reaches an internal temperature of 200 degrees, remove it from the smoker and immediately wrap it it a couple of clean towels. Leave the brisket wrapped up for at least an hour, preferably 2 hours. This allows the juices to soak back into the meat. This is referred to as resting the brisket.