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Royal New France. In 1604, the first European settlement north of Florida was established by French explorers Pierre de Monts and Samuel de Champlain, first on St. Croix Island (in present-day Maine), then at Port-Royal, in Acadia (present-day Nova Scotia). In 1608 Champlain built a fortress at what is now Quebec QuÃ©bec. city
Since all areas of the world had people living on the continents, it is unlikely any could be counted as the first. Of course, the Europeans thought theirs was the only land that mattered so when Columbus discovered North America (or at least he landed on islands in N.A.) he thought he had found a new world.