Forces That Drive Tertiary Structure. Most proteins fold into their tertiary structure in an aqueous environment-a cell is, after all, 60% water. The chemical properties of the various R-groups (sidechains) of the amino acids within the protein chain will influence the way that the protein folds in its environment.
This cold sensation will last approximately three to five minutes until a burning sensation appears. The burning sensation will fade into an ache, followed by numbness. Beware that once numbing occurs, remove and discontinue the ice. Continued application following numbing may result in tissue damage.
How long to use ice. 1  Two to 3 times per day (minimum); up to once per hour. 2  Duration varies with technique; usually 20 to 30 minutes per session. 3  Ice may continue to be useful in treatment as long as there is pain, swelling, inflammation, or spasm.