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deoxythymidinetriphosphate (dTTP) is the nucleotide in DNA that is not present in RNA. The corresponding base is thymine (T). RNA contains the nucleotide uridinetriphosphate (UTP) instead. In their incorporated forms, the will be in the monophosphate state.Note: Someone previously changed this to read Deoxythymine is the nucleotide in DNA that is not present in RNA.NA contains the nucleotide uridinetriphosphate (UTP) instead. In their incorporated forms, the will be in the monophosphate state. Note: Someone previously changed this to read Deoxythymine is the nucleotide in DNA that is not present in RNA.
In Sanger sequencing, however, a special type of dummy nucleotide is included with the regular dNTPs that surround the growing DNA strand. These special nucleotides are known as dideoxynucleotide triphosphates, or ddNTPs (Figure 2), and they lack the crucial hydroxyl group that is attached to the sugar of dNTPs.