Single-stranded oligonucleotides (ssODNs) of up to 200-nt can be ordered from Integrated DNA Technologies and other companies. Do not have the oligonucleotide PAGE-purified, as this can introduce trace chemical contaminants that reduce embryo viability.
Smoking the brisket. Place the brisket fat side up on the top rack, cover with the lid and bring the temperature up to 225Â°F, using the vents to regulate the temperature. Knowing the temp in your grill is crucial, so if your grill doesnât have a temperature gauge, purchase a digital BBQ thermometer. 4. Low and slow.
(When cooking, place the fat cap up so the juices are absorbed into the meat.) Create a dry rub using one part salt and nine parts coarse-ground pepper, then liberally coat the meat with the seasoning and massage it in. Now youâre ready to smoke a brisket thatâs a cut above.