The indirect Coombs' test is done on a sample of the liquid part of the blood. It detects antibodies that are present in the bloodstream and could bind to certain red blood cells and destroy them. A Coombs' test involves taking a sample of blood.
It is placed in the middle of the chest or thoracic cavity (middle mediastinum), posterior to the body of sternum and the second to sixth costal cartilages and anterior to the fifth to the eighth thoracic vertebrae.
On a normal person, the heart is located on the left side of the chest underneath a woman's breast or where a breast would be on a woman. There are rare birth defects, howeverâ¦, where the heart is located on the right side of the chest.