Single-stranded oligonucleotides (ssODNs) of up to 200-nt can be ordered from Integrated DNA Technologies and other companies. Do not have the oligonucleotide PAGE-purified, as this can introduce trace chemical contaminants that reduce embryo viability.
Smoking the brisket. Place the brisket fat side up on the top rack, cover with the lid and bring the temperature up to 225Â°F, using the vents to regulate the temperature. Knowing the temp in your grill is crucial, so if your grill doesnât have a temperature gauge, purchase a digital BBQ thermometer. 4. Low and slow.
Typically, when oven roasting a brisket, the temperature is set to 300 degrees F., for 30 to 45 minutes per pound. When smoking a brisket, it is cooked at 225 degrees F., for 1 1/2 hours to 2 hours per pound.