Forces That Drive Tertiary Structure. Most proteins fold into their tertiary structure in an aqueous environment-a cell is, after all, 60% water. The chemical properties of the various R-groups (sidechains) of the amino acids within the protein chain will influence the way that the protein folds in its environment.
This cold sensation will last approximately three to five minutes until a burning sensation appears. The burning sensation will fade into an ache, followed by numbness. Beware that once numbing occurs, remove and discontinue the ice. Continued application following numbing may result in tissue damage.
1 Make sure you wrap the ice in a clean, dry cloth. Applying the ice directly to your skin could cause a cold burn.  Apply the ice pack to your eye for 20 minutes each hour until you go to bed. So, you will alternate 20 minutes on, 40 minutes off, for at least the first day.