Single-stranded oligonucleotides (ssODNs) of up to 200-nt can be ordered from Integrated DNA Technologies and other companies. Do not have the oligonucleotide PAGE-purified, as this can introduce trace chemical contaminants that reduce embryo viability.
Smoking the brisket. Place the brisket fat side up on the top rack, cover with the lid and bring the temperature up to 225Â°F, using the vents to regulate the temperature. Knowing the temp in your grill is crucial, so if your grill doesnât have a temperature gauge, purchase a digital BBQ thermometer. 4. Low and slow.
After that point, youâre simply getting the meat cooked through. DO AHEAD: Brisket is best shortly off the grill, but you can still get good results smoking it up to 3 days ahead. Let cool for an hour before wrapping in foil and chilling. To serve, reheat meat, still wrapped, in a 325-degree oven until warmed through.