A general term for any factor that increases a personâs mental or physical stress; virtually all stressors can be described as related to environment (e.g., inadequate housing) or personal (e.g., health related, sexual, social or related to work).
Bovine serum albumin (also known as BSA or Fraction V) is a serum albumin protein derived from cows.It is often used as a protein concentration standard in lab experiments.t is also used as a nutrient in cell and microbial culture. In restriction digests, BSA is used to stabilize some enzymes during digestion of DNA and to prevent adhesion of the enzyme to reaction tubes, pipet tips, and other vessels.
1. Theory. TCA is an analog of acetic acid in which the three hydrogen atoms of the methyl carbon have been replaced with chlorine atoms. TCA is a relatively weak acid so it cannot hydrolyze the peptide bonds of proteins, but it does maintain an acidic pH in water.Addition of TCA to proteins in an aqueous solution disrupts the hydrogen-bonded water molecules (hydration sphere) surrounding a protein.These protein molecules no longer remain soluble and can be recovered by centrifugation.. Theory. TCA is an analog of acetic acid in which the three hydrogen atoms of the methyl carbon have been replaced with chlorine atoms. TCA is a relatively weak acid so it cannot hydrolyze the peptide bonds of proteins, but it does maintain an acidic pH in water.