The indirect Coombs' test is done on a sample of the liquid part of the blood. It detects antibodies that are present in the bloodstream and could bind to certain red blood cells and destroy them. A Coombs' test involves taking a sample of blood.
It is placed in the middle of the chest or thoracic cavity (middle mediastinum), posterior to the body of sternum and the second to sixth costal cartilages and anterior to the fifth to the eighth thoracic vertebrae.
Where Is Your Heart Located? It might be a shocker for some of you, but hearts isnât really located on the left side of your chest. It lies between the right and left lungs, in the middle of the chest and slightly towards the left of the breastbone. The heart is enclosed in the pericardium which is a double layer. This pericardium is attached to the diaphragm, spinal column and other parts via strong ligaments.