Single-stranded oligonucleotides (ssODNs) of up to 200-nt can be ordered from Integrated DNA Technologies and other companies. Do not have the oligonucleotide PAGE-purified, as this can introduce trace chemical contaminants that reduce embryo viability.
Smoking the brisket. Place the brisket fat side up on the top rack, cover with the lid and bring the temperature up to 225Â°F, using the vents to regulate the temperature. Knowing the temp in your grill is crucial, so if your grill doesnât have a temperature gauge, purchase a digital BBQ thermometer. 4. Low and slow.
Smoking the Beef Brisket. Take the brisket out of the refrigerator one to two hours before you want to put it on the smoker. Place the brisket fat side up on the smoker. The fat will render while cooking-it basically melts and releases oils into the brisket to help keep it moist while cooking.