Single-stranded oligonucleotides (ssODNs) of up to 200-nt can be ordered from Integrated DNA Technologies and other companies. Do not have the oligonucleotide PAGE-purified, as this can introduce trace chemical contaminants that reduce embryo viability.
Smoking the brisket. Place the brisket fat side up on the top rack, cover with the lid and bring the temperature up to 225Â°F, using the vents to regulate the temperature. Knowing the temp in your grill is crucial, so if your grill doesnât have a temperature gauge, purchase a digital BBQ thermometer. 4. Low and slow.
1 Barbecue brisket on a grill with charcoal requires you control the temperature with the bottom/side vents on your grill. Open the vent for more oxygen (heat). Adjust the top vent to at least half open, and leave it alone.  Put the smoker box/pouch over the coals.