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âA.C., New York, New YorkThere are a number of substitutes for buttermilk in baking. For each cup of buttermilk, you can use 1 tablespoon of white vinegar or lemon juice plus enough milk to measure 1 cup.
Buttermilk Adds Acid Element to Recipes. The most important thing to consider when preparing a substitute for buttermilk is the acid. The lactic acid in buttermilk is responsible for its characteristic flavor, texture, and leavening power. Regardless of which substitute you use, it must contain an acid component.