Single-stranded oligonucleotides (ssODNs) of up to 200-nt can be ordered from Integrated DNA Technologies and other companies. Do not have the oligonucleotide PAGE-purified, as this can introduce trace chemical contaminants that reduce embryo viability.
Smoking the brisket. Place the brisket fat side up on the top rack, cover with the lid and bring the temperature up to 225Â°F, using the vents to regulate the temperature. Knowing the temp in your grill is crucial, so if your grill doesnât have a temperature gauge, purchase a digital BBQ thermometer. 4. Low and slow.
Then, tightly wrap the brisket in a couple of layers of aluminum foil, crimping the edges to make a tight seal. Return the brisket to the smoker or grill and continue cooking until the brisket is very tenderâthe internal temperature should be 190 to 195 degrees F, about 3 hours longer.