Single-stranded oligonucleotides (ssODNs) of up to 200-nt can be ordered from Integrated DNA Technologies and other companies. Do not have the oligonucleotide PAGE-purified, as this can introduce trace chemical contaminants that reduce embryo viability.
Smoking the brisket. Place the brisket fat side up on the top rack, cover with the lid and bring the temperature up to 225Â°F, using the vents to regulate the temperature. Knowing the temp in your grill is crucial, so if your grill doesnât have a temperature gauge, purchase a digital BBQ thermometer. 4. Low and slow.
Once your smoker is maintaining 200 degrees, you are ready to place the brisket in the smoker. Smoke the Brisket. Place the brisket directly on the smoker grate with the fat side down. Maintain 200 degrees F throughout and keep the smoke going for at least 6 hours if you are not using a stick burner.nce your smoker is maintaining 200 degrees, you are ready to place the brisket in the smoker. Smoke the Brisket. Place the brisket directly on the smoker grate with the fat side down. Maintain 200 degrees F throughout and keep the smoke going for at least 6 hours if you are not using a stick burner.