The indirect Coombs' test is done on a sample of the liquid part of the blood. It detects antibodies that are present in the bloodstream and could bind to certain red blood cells and destroy them. A Coombs' test involves taking a sample of blood.
It is placed in the middle of the chest or thoracic cavity (middle mediastinum), posterior to the body of sternum and the second to sixth costal cartilages and anterior to the fifth to the eighth thoracic vertebrae.
Your heart is located under your ribcage in the center of your chest between your right and left lungs. Its muscular walls beat, or contract, pumping blood to all parts of your body. The size of your heart can vary depending on your age, size, and the condition of your heart.