Forces That Drive Tertiary Structure. Most proteins fold into their tertiary structure in an aqueous environment-a cell is, after all, 60% water. The chemical properties of the various R-groups (sidechains) of the amino acids within the protein chain will influence the way that the protein folds in its environment.
This cold sensation will last approximately three to five minutes until a burning sensation appears. The burning sensation will fade into an ache, followed by numbness. Beware that once numbing occurs, remove and discontinue the ice. Continued application following numbing may result in tissue damage.
1 As the ice melts, it will drip, so put a small towel under the area you are icing. 2  If the ice melts down so the cup is touching your skin, peel more of the cup off.  Continue for about 7 to 10 minutes. The area will feel cold at first, then it may burn, then ache, then finally become numb.