Single-stranded oligonucleotides (ssODNs) of up to 200-nt can be ordered from Integrated DNA Technologies and other companies. Do not have the oligonucleotide PAGE-purified, as this can introduce trace chemical contaminants that reduce embryo viability.
Smoking the brisket. Place the brisket fat side up on the top rack, cover with the lid and bring the temperature up to 225Â°F, using the vents to regulate the temperature. Knowing the temp in your grill is crucial, so if your grill doesnât have a temperature gauge, purchase a digital BBQ thermometer. 4. Low and slow.
Learn from the Pit Master at Wholy Smoked Briskets how to carve a whole brisket. Basic Steps: 1) Remove most of the fat by pushing it off the top plate. 2) Separate the top and bottom plates. 3) Remove as much fat from both plates as desired. 4) Align the grain on the two plates, and slice across both at once.