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In this Article. In this Article. Cortisol is a hormone your body releases when youâre under stress. Your doctor may want you to be tested to see if you have the right level of cortisol in your blood. Cortisol is made by your adrenal glands -- two small glands that sit on top of your kidneys.
Cortisol: Stress Hormone. Cortisol is a glucocorticoid hormone synthesized from cholesterol by enzymes of the cytochrome P450 family in the zona fasciculata, the middle area of the adrenal cortex (9). Regulated via the HPA axis, cortisol is the primary hormone responsible for the stress response. Expressed at the highest levels in the early morning, cortisolâs main function is to restore homeostasis following exposure to stress (10).