Hanks, who has won two Oscars but hasnât been nominated in 13 years, was overlooked for both âCaptain Phillipsâ and âSaving Mr. Banks.â. SNUB: âInside Llewyn Davis.â Even with nine best picture nominees, the Coen brothers, a perennial Academy favorite, lost out this time in the top category with their musical dramedy.
Anti-parallel strands. The DNA molecule is composed of two strands held together by hydrogen bonds. A single strand is different at its two ends. One end is called 5' (5 prime), the other is called 3' (3 prime). The names come from the notation for the two sugar carbon atoms which participate in the phosphodiester bonds. The different strands in the helix run in opposite (anti-parallel) directions. At each end of the double helix, one strand is 5' and the other is 3'. In the illustration, the 3' end is shown with a red arrowhead and the 5' end with the yellow connector.
DNA gyrase, or simply gyrase, is an enzyme within the class of topoisomerase (Type II topoisomerase) that relieves strain while double-stranded DNA is being unwound by helicase. The enzyme causes negative supercoiling of the DNA or relaxes positive supercoils. It does so by looping the template so as to form a crossing, then cutting one of the double helices and passing the other through it before releasing the break, changing the linking number by two in each enzymatic step.