To get the whole picture and have a framework on which to build your business you also need a strategic plan. First, lets look at the difference between a business and strategic plan. In the simplest terms: A business plan covers the âwhoâ and âwhatâ of the business.
The standard temperature lapse rate is the rate at which we expect air to be colder, the higher we go. The rate is about 2 Fahrenheit degrees per 1000 feet of altitude, which coincidentally happens to be about 1 Celsius degree per 100 meters.
Notes. 1  In the actual PCR run use an annealing temperature 5Â°C lower than the lower Tm given by the calculator. 2  If high DMSO concentration is used, the annealing temperature determined by the guidelines above must be lowered, as DMSO decreases the melting point of the primers. If high DMSO concentration is used, the annealing temperature determined by the guidelines above must be lowered, as DMSO decreases the melting point of the primers. 2  It has been reported that 10% DMSO decreases the melting temperature by 5.5â6.0Â°C. 2.