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The most. significant pathogens are Streptococcus pyogenes (Lancefield Group A), Streptococcus. agalactiae (Lancefield Group B) a nd Streptococcus pneumoniae. Some streptococci (fo rmerly. group D) n ow placed in the genus Enterococcus are of medical significance because they are.
One way to differentiate beta-hemolytic group A Streptococcus from other beta-hemolytic streptococci is by determination of their sensitivity to bacitracin. Streptococcus pyogenes (group A beta-hemolytic) is sensitive to bacitracin and will not grow around the antibiotic-containing disc.