During this handshake, the client and server agree on various parameters used to establish the connection's security: 1  The handshake begins when a client connects to a TLS-enabled server requesting a secure connection and the client presents a list of supported cipher suites (ciphers and hash functions).
Allele frequency. Allele frequency is a term of population genetics that is used in characterizing the genetic diversity of a species population, or equivalently the richness of its gene pool.
For the following discussion: Variant allele frequency = read count supporting mutant base / total read count at that position For heterozygous somatic variants we can expect a tumor variant allele frequency of 50% and a normal variant allele frequency of 0%.Of course there will always be some variability due to some of the technical artifacts already discussed (random sequencing errors, etc.).n this case somatic variants that exist only in the sub-clone will have a substantially reduced tumor variant allele frequency. When both purity and heterogeneity are in play, interpreting tumor variant allele frequencies just gets more and more complicated ...