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Intrinsic factor. Intrinsic factor (IF), also known as gastric intrinsic factor (GIF), is a glycoprotein produced by the parietal cells of the stomach. It is necessary for the absorption of vitamin B12 (cobalamin) later on in the small intestine. In humans, the gastric intrinsic factor protein is encoded by the GIF gene.
A decreased vitamin B12 level and/or increased methylmalonic acid and homocysteine levels as well as a positive test result for intrinsic factor antibodies and/or parietal cell antibodies means that it is likely that the person tested has pernicious anemia. A negative test result does not necessarily mean that a person does not have pernicious anemia. At least 10% of those affected will not have parietal cell antibodies. Parietal cell antibodies are not as specific as intrinsic factor antibodies. About 90% of those with pernicious anemia will have parietal cell antibodies, but they may also be found in: