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Answer by Surfbanana. Confidence votes 22. Helicase and RNA polymerase separate DNA strands by breaking the hydrogen bonds between complementary bases. Helicase parts the strands of DNA during DNA replication, and RNA polymerase parts them during transcription. The enzyme that separates DNA in called DNA helicases. There are two of them that work away from the origin of replication, creating in bubble in the DNA molecule.
Original conversation. User: Describe what DNA replication is, and how it relates to the DNA polymerase enzymes. In your own wordsWeegy: The double helix of the DNA unwinds. Then, the enzyme helicase 'unzips' the DNA by breaking the hydrogen bonds.hen, the enzyme DNA Polymerase pairs each of the two strands with the correct nitrogenous bases. [ The top strand is called the 'leading strand' and the bottom strand is called the 'lagging strand'. Then the enzyme ligase glues together the different fragments of the lagging strand of DNA.