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Answer by Surfbanana. Confidence votes 22. Helicase and RNA polymerase separate DNA strands by breaking the hydrogen bonds between complementary bases. Helicase parts the strands of DNA during DNA replication, and RNA polymerase parts them during transcription. The enzyme that separates DNA in called DNA helicases. There are two of them that work away from the origin of replication, creating in bubble in the DNA molecule.
For DNA replication, first it needs to unwind the double helix. There are two types of bondings in the strand-double bonds between Adenine and Thymine nitrogenous bases and triple bonds between Guanine and Cytosine bases.These bonds are broken during replication. After thus separating the two strands of the DNA, replication begins.Considering one strand, - it will have now a series of nitrogenous bases-complimentary bases are attached to these existing bases, i.e. if G existing on our separated strand, C is attached to this G and this is continued for all the bases.fter thus separating the two strands of the DNA, replication begins. Considering one strand, - it will have now a series of nitrogenous bases-complimentary bases are attached to these existing bases, i.e. if G existing on our separated strand, C is attached to this G and this is continued for all the bases.