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A lack of appetite is usually one of the earliest signs that your dog might be pregnant. Not all females go through this doggie version of morning sickness, but a small percentage of dogs will eat less during the first few weeks of gestation and will usually make up for it later in the pregnancy.
There is no blood or urine test commonly used to diagnose pregnancy in dogs, but from 3 to 4 weeks into the gestation period, a vet should be able to tell if your dog is pregnant by feeling her abdomen. A more accurate yet more costly way of checking whether a dog is pregnant is by ordering an ultrasound.