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Answer by Surfbanana. Confidence votes 22. Helicase and RNA polymerase separate DNA strands by breaking the hydrogen bonds between complementary bases. Helicase parts the strands of DNA during DNA replication, and RNA polymerase parts them during transcription. The enzyme that separates DNA in called DNA helicases. There are two of them that work away from the origin of replication, creating in bubble in the DNA molecule.
(It is the 3Â´ -OH group thatis required to create the next phosphodiester bond.) The RNA is subsequentlyremoved and replaced with DNA so that no gaps exist in the final product. 4. Helicases are enzymes that disrupt the hydrogen bonds that hold the two DNAstrands together in a double helix.This breakage is required for both RNA andDNA synthesis. Topoisomerases are enzymes that create and relax supercoiling inthe DNA double helix.It is the 3Â´ -OH group thatis required to create the next phosphodiester bond.) The RNA is subsequentlyremoved and replaced with DNA so that no gaps exist in the final product. 4. Helicases are enzymes that disrupt the hydrogen bonds that hold the two DNAstrands together in a double helix.