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A Barr body is the inactivated X chromosome that is usually found in the nuclei of female somatic cells. It can also occur in males when there is an abnormality and the male is XXY instead of the normal XY, a condition known as Klinefelter's syndrome.
[The other dark bodies within the nucleus are nucleoli, which represent repetitive rDNA genes]. Barr body testing was used in the 1968 Olympic games in an effort to detect male athletes supposedly trying to pass as females to gain a competitive advantage. Teams from eastern Europe were particularly suspect.