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Answer by Surfbanana. Confidence votes 22. Helicase and RNA polymerase separate DNA strands by breaking the hydrogen bonds between complementary bases. Helicase parts the strands of DNA during DNA replication, and RNA polymerase parts them during transcription. The enzyme that separates DNA in called DNA helicases. There are two of them that work away from the origin of replication, creating in bubble in the DNA molecule.
The enzyme helicase catalyzes the unwinding and the breaking of H-bonds to create a separated section of DNA. The open section is known as a replication bubble and in contains two replication forks.Many different replication bubbles are formed along the DNA structure.he enzyme helicase catalyzes the unwinding and the breaking of H-bonds to create a separated section of DNA. The open section is known as a replication bubble and in contains two replication forks.