I have a '5 Way Reflector' that I want to use in my photography, which includes the following 5 options: 1  Gold reflective. 2  Silver reflective. 3  Gold/silver alternating pattern reflective. 4  White. 5  Scrim/opaque. 6  Black*.
The endoplasmic reticulum (ER) resident PKR-like kinase (PERK) is necessary for Akt activation in response to ER stress. We demonstrate that PERK harbors intrinsic lipid kinase, favoring diacylglycerol (DAG) as a substrate and generating phosphatidic acid (PA). This activity of PERK correlates with activation of mTOR and phosphorylation of Akt on Ser473. PERK lipid kinase activity is regulated in a phosphatidylinositol 3-kinase (PI3K) p85Î±-dependent manner. Moreover, PERK activity is essential during adipocyte differentiation.
In vitro kinase assays are often performed using 32 P- or 33 P- labeled ATP. In this assay, you will measure transfer of the gamma phosphate from ATP (or sometimes GTP) to a substrate. Radioactive assays can be used for kinetics or screening. The assay can be compatible with essentially any kind of substrate, including peptides, proteins, lipids, and small molecules. Radioactive biochemical kinase assays can be performed in several different formats.