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A lack of appetite is usually one of the earliest signs that your dog might be pregnant. Not all females go through this doggie version of morning sickness, but a small percentage of dogs will eat less during the first few weeks of gestation and will usually make up for it later in the pregnancy.
The quickest way to tell if the dog is pregnant is to take the dog to a vet and have blood drawn. It is VERY important to do this as sometimes what you may think is a pregnancy is a closed Pyometria, which can of cousre kill the bitch and is an automatic spay.