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Answer by Surfbanana. Confidence votes 22. Helicase and RNA polymerase separate DNA strands by breaking the hydrogen bonds between complementary bases. Helicase parts the strands of DNA during DNA replication, and RNA polymerase parts them during transcription. The enzyme that separates DNA in called DNA helicases. There are two of them that work away from the origin of replication, creating in bubble in the DNA molecule.
Daughter strand-Refers to the newly synthesized strand of DNA that is copied via the addition of complementary nucleotides from one strand of pre-existing DNA during DNA replication. DNA Helicase-The enzyme responsible for separating the two strands of DNA in a helix so that they can be copied during DNA replication.rimase-The enzyme responsible for initiating synthesis of RNA primers on the lagging strand during DNA replication. Holoenzyme-A term used to describe a collection of different enzymes that work together in a given process such as DNA replication.