In which case, it depends on the type of inhibitor. A competitive inhibitor has a structure similar to the substrate, hence would bind to the active site as well, competing with the substrate for the enzyme active sites, decreasing enzymatic activity. A non-competitive inhibitor binds to the allosteric site of the enzyme, causing a structural change in the enzyme active site shape.
There's a difference between the effects of cold-brewed coffee vs. hot-brewed coffee: because different chemical reactions are achieved at higher temperatures, cold-brewed coffee will have a smoother caffeine effect and overall less acidity, which some people experience as part of the kick..
Coffee Beer: How to Cold Brew Coffee. Making a quality cup of cold brew coffee is a skill every homebrewer should have in their arsenal of DIY tricks. But it isnât as simple as refrigerating a pot of hot-brewed joe. Cold brew coffee is not only great for making the best cup of iced coffee youâll ever have, itâs also the best way to add coffee to your next homebrew.