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I inoculated nutrient agar with bacillus subtilis and it made a dark green colour on agar plates.
Nutrient agar is a general purpose medium supporting growth of a wide range of non-fastidious organisms. It typically contains (mass/volume): 1  0.5% Peptone. 2  0.3% beef extract / yeast extract. 3  1.5% agar. 4  0.5% NaCl. 5  distilled water. 6  pH adjusted to neutral (6.8) at 25 Â°C.