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Vial: Once the vial is punctured, it is OPEN. Once you stick a needle in the vial, it is OPEN. OPEN vials can be stored in the fridge or at CONTROLLED room temperature. Regardless of where it is stored, OPEN insulin will only last 28 days before it must be thrown in the trash.
I use NovoLog. Unopened vials or pens should be refrigerated but I was advised that I could keep the NovoLog Flexpen I was using at room temp and then to discard it after 28 days. Now, I'm using NovoLog vials to refill my new insulin pump every 3 days. I keep the opened vial in the fridge and take it out so it warms to room temp before refilling my pump reservoir. My pump trainer said that insulin at room temp is less likely to have bubbles in it and you don't want bubbles in your pump tubing.