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I inoculated nutrient agar with bacillus subtilis and it made a dark green colour on agar plates.
Characteristics of the components used in nutrient agar/broth. 1  Beef extract is an aqueous extract of lean beef tissues. 2  Peptone is made by digesting proteinaceous materials e.g., meat, casein, gelatin, using acids or enzymes. 3  Agar is a complex carbohydrate obtained from certain marine algae. It is used as a solidifying agent for media and does not have any nutritive value. Agar gels when the temperature of media reaches 45Â°C and melts when the temperature reaches 95 Â°C. Preparation of Nutrient Agar