For Best Results. Apply the Upper Brush against the top lashes and sweep from root to tip until a clean, voluminous look is achieved, followed by the lower lash line using the Micro Brush. Do not let dry between coats. Removes with soap and water or with MaybellineÂ® Clean Express!Â® Eye Makeup Remover.
Neutral peptides usually dissolve in organic solvents. First, try to add a small amount of acetonitrile, methanol, or isopropanol. For very hydrophobic peptides, try to dissolve the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
Trichloroacetic acid (TCA) precipitation of proteins is commonly used to concentrate protein samples or remove contaminants, including salts and detergents, prior to downstream applications such as SDS-PAGE or 2D-gels.. Theory. TCA is an analog of acetic acid in which the three hydrogen atoms of the methyl carbon have been replaced with chlorine atoms. TCA is a relatively weak acid so it cannot hydrolyze the peptide bonds of proteins, but it does maintain an acidic pH in water.