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Vial: Once the vial is punctured, it is OPEN. Once you stick a needle in the vial, it is OPEN. OPEN vials can be stored in the fridge or at CONTROLLED room temperature. Regardless of where it is stored, OPEN insulin will only last 28 days before it must be thrown in the trash.
My pharmacist advised that the bottle was good for up to 60 days after opening as long as its kept cool. I find no drop off of effectiveness going to 60 days. I believe it is a waste to through it out after 28 days because I still have over 80% of the bottle left after 28/30 days. As stated, and from what I was told. all insulin should be descarded after 30 days of first use.