Therefore, whenever a ddNTP is added to a growing DNA strand, it is unable to chemically bind with the next nucleotide in the chain, and the DNA strand stops growing. Figure 3: By adding together information about all of the truncated strands, researchers can determine the nucleotide sequence of the DNA target.
Functions of the Bladder. Temporary store of urine â The bladder is a hollow organ. The walls are very distensible, with a folded internal lining (known as rugae), this allows it to hold up to 600ml. Assists in the expulsion of urine â During voiding, the musculature of the bladder contracts, and the sphincters relax.
Structure. The bladder wall is made up of 3 layers. 1  The urothelium lines the inside of the bladder, ureters and urethra. 2  It is made up of urothelial cells, or transitional cells. 3  It is also called the transitional epithelium. 4  The lamina propria is a layer of connective tissue. The lamina propria is also called suburothelial connective tissue or subepithelial tissue. 2  The muscularis is the outer layer of the bladder. 3  It is made up of 3 layers of smooth muscle tissue (bladder muscle) called the inner longitudinal, middle circular and outer longitudinal muscles.