In which case, it depends on the type of inhibitor. A competitive inhibitor has a structure similar to the substrate, hence would bind to the active site as well, competing with the substrate for the enzyme active sites, decreasing enzymatic activity. A non-competitive inhibitor binds to the allosteric site of the enzyme, causing a structural change in the enzyme active site shape.
There's a difference between the effects of cold-brewed coffee vs. hot-brewed coffee: because different chemical reactions are achieved at higher temperatures, cold-brewed coffee will have a smoother caffeine effect and overall less acidity, which some people experience as part of the kick..
Research has also investigated the effects of coffee on neurodegenerative disorders such as Alzheimerâs and Parkinsonâs disease. 1  Regular coffee drinking throughout life may slow down age-related cognitive decline, especially in women and this protective effect increases with age 20,21,22,23.