A referee makes recommendations to the judge or court that appoints the referee but generally does not issue enforceable orders. A referee generally cannot render judgment in a case. The referee's general duty is to provide a report to the appointing judge on the issues of fact or law that prompted the referee's appointment.
Autoclave Overview. Learn the purpose and limitations of autoclaves, types of cycles, and procedures for safe and effective autoclaving. Purpose. Autoclaving, sometimes called steam sterilization, is the use of pressurized steam to kill infectious agents and denature proteins. This kind of wet heat is considered the most dependable method of sterilizing laboratory equipment and decontaminating biohazardous waste.
The following are basic instructions for autoclave use but do not replace the manufacturer's operating instructions and hands-on training. Before using any autoclave for the first time, read and thoroughly understand the owner's manual because many makes and models have unique characteristics. Place the items to be autoclaved in the chamber. Check the drain screen to make sure that it is not plugged or obstructed. For bench top units that do not have inline steam, check and fill the reservoir with deionized water to the fill line (see manufacturer's instructions). Close and seal the autoclave door. On the autoclave keypad, or dials, select: