A referee makes recommendations to the judge or court that appoints the referee but generally does not issue enforceable orders. A referee generally cannot render judgment in a case. The referee's general duty is to provide a report to the appointing judge on the issues of fact or law that prompted the referee's appointment.
Autoclave Overview. Learn the purpose and limitations of autoclaves, types of cycles, and procedures for safe and effective autoclaving. Purpose. Autoclaving, sometimes called steam sterilization, is the use of pressurized steam to kill infectious agents and denature proteins. This kind of wet heat is considered the most dependable method of sterilizing laboratory equipment and decontaminating biohazardous waste.
4. Run the autoclave. The autoclave machine should run for a certain period of time at a specific temperature and pressure. Wrapped instruments should be in the autoclave at 250 degrees for 30 minutes at 15 PSI or 273 degrees for 15 minutes at 30 PSI.Once the machines has run, you need to open the door a small bit to let the steam out.. Run the autoclave. The autoclave machine should run for a certain period of time at a specific temperature and pressure. Wrapped instruments should be in the autoclave at 250 degrees for 30 minutes at 15 PSI or 273 degrees for 15 minutes at 30 PSI.