For Best Results. Apply the Upper Brush against the top lashes and sweep from root to tip until a clean, voluminous look is achieved, followed by the lower lash line using the Micro Brush. Do not let dry between coats. Removes with soap and water or with MaybellineÂ® Clean Express!Â® Eye Makeup Remover.
Sonication can be used to enhance solubility. 1  First, assign a value of â1 to each acidic residue (Asp [D], Glu [E], and the C-terminal âCOOH). 2  If the overall charge of the peptide is positive, the peptide is basic. 3  If the overall charge of the peptide is negative, the peptide is acidic. Acidic peptides might be soluble in PBS (pH 7.4). If this fails, add a small amount of basic solvent such as 0.1 M ammonium bicarbonate to dissolve the peptide, and then add water to the desired concentration.
Gel electrophoresis can also be used for separation of nanoparticles. Gel electrophoresis uses a gel as an anticonvective medium and/or sieving medium during electrophoresis, the movement of a charged particle in an electrical field.olyacrylamide gel electrophoresis (PAGE) is used for separating proteins ranging in size from 5 to 2,000 kDa due to the uniform pore size provided by the polyacrylamide gel. Pore size is controlled by modulating the concentrations of acrylamide and bis-acrylamide powder used in creating a gel.