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I inoculated nutrient agar with bacillus subtilis and it made a dark green colour on agar plates.
Sterilized the medium by autoclaving (121Â°C for 15 min) Dispense the medium in to tubes or plates. Left the agar medium to solidify and store. Determine the pH of the medium (pH 6.8 +/- 0.2) with a pH meter and adjust if necessary. Uses of Nutrient Agar/broth Un-inoculated Nutrient Agar. 1.