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In the most basic terms, genome is the genetic material of an organism. It is what tells the organism what to be and how to be. The genome is coded in DNA, or in RNA for many types of viruses. The term, genome, was coined in the 1920, as a portmanteau of gene and chromosome.
A gene is a segment of DNA that codes for a polypeptide or for a molecule of RNA, such as a transfer RNA (tRNA) or ribosomal RNA (rRNA). The genes in the nucleus are in chromosomes. A chromosome consists of a molecule of DNA (two after replication of the DNA) and associated proteins. Genes are segments of DNA molecules.