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Inside the mitochondria is where most of your ATP are created, but some ATP can be made in the cytoplasm through a process called substrate-level phosphorylation. This is a process of forming ATP by the physical addition of a phosphate group to ADP.
Although activation of many protein kinases is mediated by phosphorylation at a critical site on the activation loop by a heterologous protein kinase , phosphorylation at this site in the catalytic subunit of PKA until now has been poorly understood both in terms of the specific kinase that mediates this phosphorylation and in terms of the overall location and regulation of this process in cells.