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Inside the mitochondria is where most of your ATP are created, but some ATP can be made in the cytoplasm through a process called substrate-level phosphorylation. This is a process of forming ATP by the physical addition of a phosphate group to ADP.
Enzymes operate by binding to a substrate or substrates temporarily, lowering the activation energy necessary for a reaction to take place. The catalytic (active) site is the portion of the enzyme where substrate binding occurs. Each enzyme is specific for one or more substrate types. Two different theories about how enzyme-substrate binding exist. In the induced-fit (key and lock) model (a), the active site of an enzyme has a shape specific to the substrate.