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Pseudomonas aeruginosa reduces sulfur and nitrate, and has a characteristic green color. The API 20E Identification System is a test strip that can be used to identify Proteus vulgaris versus other bacteria. An even easier way to test this is the gelatinase test.Or, slant the bacteria on a Triple Sugar Iron slant or place them in a SIM agar deep. P. vulgaris tests positive for Hydrogen Sulfide production, changing the media to a black color around the organism, while P. aeruginosa does not produce Hydrogen Sulfide.seudomonas aeruginosa reduces sulfur and nitrate, and has a characteristic green color. The API 20E Identification System is a test strip that can be used to identify Proteus vulgaris versus other bacteria. An even easier way to test this is the gelatinase test.
Pseudomonas aeruginosa is widespread in nature, inhabiting soil, water, plants and animals (including humans). Identification of P. aeruginosa often includes identifying the production of both pyocyanin and fluorescein. Its optimum temperature for growth is 37Â°C, and it is able to grow at temperatures as high as 42Â°C.