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DNA is a polar molecule because it has a negatively (-) charged phosphate group attached to the 5' end of the molecule and a negatively charge hydroxl group (OH) attached to t â¦ he 3' prime end of the molecule. Thus, it is a POLAR molecule which allows it to interact in an aqueous environment.NA is a polar molecule because it has a negatively (-) charged phosphate group attached to the 5' end of the molecule and a negatively charge hydroxl group (OH) attached to t â¦ he 3' prime end of the molecule.
It makes it easier to load the samples and visually track the migration of DNA through the gel. 4. Explain why DNA has an overall negative charge. Phosphate groups in the DNA backbone carry negatively-charged oxygen molecules giving the. phosphate-sugar backbone of DNA an overall negative charge. 5.