Our MSK physicians specialize in bone and joint imaging, spinal imaging, metabolic bone disease, and osteoporosis. Imaging techniques include plain films, CT, Ultrasound, and MRI. For diagnostic purposes, the division also performs plain films, arthrography, bone biopsies, and peripheral soft tissue lesions.
If you want to isolate plasmid DNA, you crack your cells open and carry out a miniprep, trying very hard not to get any contaminating genomic DNA in your sample.If you want genomic DNA, you crack your cells open in a different way and try to isolate as much of the stuff as possible.f you want to isolate plasmid DNA, you crack your cells open and carry out a miniprep, trying very hard not to get any contaminating genomic DNA in your sample.
Just kidding. For our purposes, we mainly use plasmids as cloning vectors. And as such, a good plasmid has the following: 1  oriC, an origin of replication. 2  Gotta start making new plasmid somewhere. a multiple cloning site (MCS) inside a scorable marker. 2  The MCS allows us to cut the plasmid, insert new DNA, and re-ligate; the scorable marker allows us to see if the plasmid does indeed have an insert, because the insert will disrupt expression of the marker.