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Inside the mitochondria is where most of your ATP are created, but some ATP can be made in the cytoplasm through a process called substrate-level phosphorylation. This is a process of forming ATP by the physical addition of a phosphate group to ADP.
Oxidative phosphorylation occurs in the mitochondria, and the two reactions (oxidation of NADH or FADH 2 and phosphorylation to generate ATP) are coupled by a proton gradient across the inner membrane of the mitochondria (Figure 9).he oxidation reaction for NADH has a larger, but negative, D G than the positive D G required for the formation of ATP from ADP and phosphate. This set of coupled reactions is so important that it has been given a special name: oxidative phosphorylation.