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Meatloaf is done when a meat thermometer inserted in its center reads 160 degrees Fahrenheit. Ground beef used for food items like hamburgers or meatloaf should be at this temperature after it is cooked.
Best thing to do is to get a thermometer, once the meatloaf hits 160-165F internally then it should be moist and juicy and cooked.   See my blog for the best turkey meatloaf recipe ever and for amazing and original turkey recipes.  http://www.loveturkeymeat.info.eef should be cooked 145 (med. rare) to 165 (med) for things like meatloaf, you wanna pull the loaf out when it's about 5 degrees below the desired temp.