Our MSK physicians specialize in bone and joint imaging, spinal imaging, metabolic bone disease, and osteoporosis. Imaging techniques include plain films, CT, Ultrasound, and MRI. For diagnostic purposes, the division also performs plain films, arthrography, bone biopsies, and peripheral soft tissue lesions.
If you want to isolate plasmid DNA, you crack your cells open and carry out a miniprep, trying very hard not to get any contaminating genomic DNA in your sample.If you want genomic DNA, you crack your cells open in a different way and try to isolate as much of the stuff as possible.f you want to isolate plasmid DNA, you crack your cells open and carry out a miniprep, trying very hard not to get any contaminating genomic DNA in your sample.
the splicing 2gether of the plasmid with the gene inserted into it is catalysed by the enzyme DNA ligase. The plasmid is re-inserted into the bacteria by mixing with calcium chloride in a test tube.he recombitant plasmid, or molecular clone, is introduced into a bacterial cell by adding the DNA to a bacterial culture. Under the right condition some bacteria will take upthe plasmid from solution by the process TRANFORMATION; THIS IS THE PREPARATION OF CLONE upto this point. NOW, let's start CLONNING the gene......