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The proton motive force arises from a combination of the concentration gradient of protons and a voltage gradient across the membrane (the protons are positively charged, so an imbalance of protons means that the side of the membrane with more protons has a positive charge relative to the side with fewer protons).
Furthermore, the sleeper effect did not occur when any of the four requirements were not met. According to the dissociation interpretation, a sleeper effect appears to happen when a convincing message is conferred with a discounting cue (such as a low-credible source or counterargument). A sleeper effect occurs because of an impulsive dissociation of a message and a discounting cue over time (contrasting to a simple forgetting of a source). First identified. The sleeper effect was first identified in U.S. soldiers during World War II, after attempts to change their opinions and morals.