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The cell's cytoplasm is a non-membraned bound organelle. It covers a large area and all the cell's membrane bound organelles are suspended in it. Glycolysis does not require oxygen or any membrane bound cellular organelle for it to run to completion.It requires enzymes and ATP; so where ever glucose is in the cytoplasm the process can begin.t covers a large area and all the cell's membrane bound organelles are suspended in it. Glycolysis does not require oxygen or any membrane bound cellular organelle for it to run to completion.
Best Answer: PYRUVATE Glycolysis occurs in the cytoplasm in the absence of O2and involves the following: 1. Two molecules of ATP are use to phosphorylate glucose and start glycolysis 2. The phosphorylated molecule is then broken down in a series of reactions into two three carbon molecules (lysis). 3. Two molecules of NAD+ capture and are reduced to 2 molecules of NADH + H+ 3.